Claim Missing Document
Check
Articles

Found 27 Documents
Search

Antibacterial Activity of Endophytic Bacteria of Seagrass Symbiont Enhalus acoroides from Tiwoho Waters, North Minahasa Purniasih, Ni Komang Pitri; Ginting, Elvy Like; Wullur, Stenly; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Pratasik, Silvester Benny
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42485

Abstract

Nutrient-poor marine bacteria are often found to form a living mechanism by associating with other marine organisms such as seagrass Enhalus acoroides. Seagrass serves as a habitat for marine biota and is known to produce bioactive compounds. Endophytic and epiphytic microbes that are symbiotic in seagrass have the ability to produce bioactive compounds similar to the bioactive compounds produced by their host. Therefore, this study aimed to obtain isolates of symbiont bacteria and to test their antibacterial activity.  The symbiotic bacteria isolates were tested for their antibacterial activity using gram-negative bacteria such as E. coli and S. typhi, and gram-positive bacteria such as S. aureus and S. mutans. Antibacterial activity test was carried out using the paper disc method. Observation of antibacterial activity was carried out for 3x24 hours by observing the growth of the resulting inhibition zone. The zone of inhibition was measured to determine the diameter and strength of the bioactive compounds produced by symbiotic bacteria.A total of six isolates of symbiotic bacteria were obtained from the seagrass Enhalus acoroides with varied morphological characteristics. The six isolates of symbiont bacteria showed antibacterial activity and could inhibit and kill pathogenic bacteria. Two of the six isolates of symbiotic bacteria that produced the largest diameter of the inhibition zone were isolates E (Ep1) 3,5 mm and F (En3) 4 mm in S. mutans test bacteria.Keywords: Antibacterial, Isolation, Enhalus acoroides, Symbionts, Tiwoho AbstrakBakteri laut yang miskin nutrisi banyak dijumpai membentuk mekanisme hidup dengan cara berasosiasi dengan organisme laut lainnya seperti lamun Enhalus acoroides. Lamun berfungsi sebagai habitat biota laut dan diketahui dapat menghasilkan senyawa bioaktif. Mikroba endofit dan epifit yang bersimbiosis pada lamun mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif yang serupa dengan senyawa bioaktif yang diproduksi inangnya. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri simbion serta menguji aktivitas antibakterinya.  Isolat bakteri simbion diuji aktivitas antibakterinya menggunakan bakteri uji gram negatif seperti E. coli dan S. typhi, dan bakteri uji gram positif seperti S. aureus dan S. mutans. Uji aktivitas antibakteri dilakukan menggunakan metode kertas cakram. Pengamatan aktivitas antibakteri dilakukan selama 3x24 jam dengan mengamati pertumbuhan zona hambat yang dihasilkan. Zona hambat diukur untuk mengetahui diameter dan kekuatan senyawa bioaktif yang dihasilkan oleh bakteri simbion.  Sebanyak enam isolat bakteri simbion yang didapatkan dari lamun Enhalus acoroides dengan karakteristik morfologi yang bevariasi. Keenam isolat bakteri simbion menunjukkan adanya aktivitas antibakteri dan dapat menghambat serta membunuh bakteri pathogen. Dua dari enam isolat bakteri simbion menghasilkan diameter zona hambat terbesar adalah isolat E (Ep1) 3,5 mm dan F (En3) 4 mm pada pada bakteri uji S. mutans.Kata kunci: Antibakteri, Isolasi, Enhalus acoroides, Simbion, Tiwoho
Cardinal Fish Otolith Biometrics Banggai Pterapogon kauderni Koumans, 1933 In the Front Waters of Dudepo TPI Dudepo, South Bolaang Mongondow Regency and in the Lembeh Strait, Bitung City Melani, Hellen; Rondonuwu, Ari B.; Sangari, Joudy R.R; Manginsela, Fransine B.; Pratasik, Silvester B.; Undap, Suzanne L.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.44002

Abstract

This study aims to determine the biometric comparison of otoliths of Banggai cardinal fish based on gender and to determine the comparison of otolith biometrics at two locations in the Waters Front of TPI Dudepo Kab. South Bolaang Mongondow and in the Lembeh Strait, Bitung City. Data collection in the field using the roaming survey method. Fishing is done by snorkelling, catching a minimum of 50 individuals representing each size class using Chang net/Sibu fishing gear. The differences in otoliths in male and female sexes have seven different otolith characters in otolith length (OL), otolith width (OW), otolith area (OA) and otolith perimeter (OP), roundness (Rnd), ellipticity (Ell) and aspect ratio (Ar), and three characters that do not show significant differences, namely form factor (Ff), circularity (Cir), rectangularity (Rec). The differences in otoliths based on location in front of the Dudepo TPI and in the Lembeh Strait have seven different otolith characters in otolith length (OL), otolith width (OW), otolith area (OA) and otolith perimeter (OP), roundness (Rnd), ellipticity (Ell). and aspect ratio (Ar), and three characters that do not show significant differences, namely form factor (Ff), circularity (Cir), rectangularity (Rec). Keywords: Biometrics, cardinal proud fish, comparison Abstrak Penelitian ini bertujuan untuk mengetahui perbandingkan biometrik otolit ikan kardinal banggai berdasarkan jenis kelamin dan mengetahui perbandingan biometrik otolit pada dua lokasi di Perairan Depan TPI Dudepo Kab. Bolaang Mongondow Selatan dan di Selat Lembeh Kota Bitung. Pengambilan data di lapangan menggunakan metode survei jelajah. Penangkapan ikan dilakukan dengan penyelaman snorkling, penangkapan minimal 50 individu yang mewakili setiap kelas ukuran dengan alat tangkap Chang net/Sibu. Perbedaan otolit pada jenis kelamin jantan dan jenis kelamin betina memiliki tujuh perbedaan karakter otolit pada panjang otolit (OL), lebar otolit (OW), area otolit (OA) dan perimeter otolit (OP), roundness (Rnd), ellipticity (Ell) dan aspect ratio (Ar), dan tiga karakter yang tidak menunjukkan perbedaan yang signifikan, yaitu form factor (Ff), circularity (Cir), rectangularity (Rec). Perbedaan otolit berdasarkan lokasi di depan TPI Dudepo dan di Selat Lembeh memiliki tujuh perbedaan karakter otolit pada panjang otolit (OL), lebar otolit (OW), area otolit (OA) dan perimeter otolit (OP), roundness (Rnd), ellipticity (Ell) dan aspect ratio (Ar), dan tiga karakter yang tidak menunjukkan perbedaan yang signifikan, yaitu form factor (Ff), circularity (Cir), rectangularity (Rec). Kata kunci: Biometrik, ikan banggai kardinal, perbandingan
Morphological characteristics of dolphinfish Coryphaena hippurus Linnaeus 1758 landed in Kalinaun Village, East of Likupang District, North Sulawesi Mantiri, Nicola R. K.; Tilaar, Ferdinand F.; Pratasik, Silvester B.; Sinjal, Henky; Lohoo, Anneke V.; Rondonuwu, Ari B.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.44916

Abstract

This study aimed to determine the size group of fish caught, compare the morphometric characteristics between sexes, and describe the meristic characteristics of the dolphinfish Coryphaena hippurus. This study used a descriptive method. The morphometric characteristics were measured using a ruler and tape at the accuracy of 1 mm and 5 m, respectively, while the meristic characteristics were obtained by counting the number of spines. This study found a total of 57 individuals of the dolphinfish that had a size range of 48–89 cm, the male from 48 to 95 cm, and the females from 49 to 84 cm, respectively. Four morphometric parameters of male and 2 parameters of female dolphinfish could be used to predict the total length. The dolphinfish also had 59 dorsal soft rays, 25 soft pelvic rays, 22 soft pectoral rays, 26 soft anal rays, and 48 hard caudal rays. Keywords: descriptive, morphometric, meristic, size. Abstrak Penelitian ini bertujuan untuk mengetahui kelompok ukuran ikan yang tertangkap, membandingkan karakteristik morfometrik antar jenis kelamin, dan mendeskripsikan karakteristik meristik ikan Lemadang Coryphaena hippurus. Penelitian ini menggunakan metode deskriptif. Karakteristik morfometrik diukur menggunakan penggaris dan meteran dengan ketelitian masing-masing 1 mm dan 5 m, sedangkan perhitungan meristik dilakukan dengan cara menghitung jari-jari sirip. Penelitian ini menemukan sebanyak 57 individu ikan Lemadang yang memiliki kisaran ukuran 48-89 cm, jantan 48-95 cm, dan betina berkisar 49-84 cm. Empat parameter morfometrik ikan Lemadang jantan dan 2 parameter ikan lemadang betina dapat digunakan untuk menduga panjang total. Ikan Lemadang juga memiliki 59 jari-jari lemah sirip punggung, 25 jari-jari lemah sirip perut, 22 jari-jari lemah sirip dada, 26 jari-jari lemah sirip dubur, dan 48 jari-jari keras sirip ekor. Kata kunci: deskriptif, morfometrik, meristik, ukuran.
Length-Weight Relationship and Reproduction of Spotcheek emperor Lethrinus rubrioperculatus Sato, 1978 at Napo Nain Likupang North Sulawesi Laura, Azhar; Kambey, Alex D.; Bataragoa, M.Sc, Dr. Ir. Nego E.; Pratasik, Silvester B.; Lohoo, Anneke V.; Kusen, Janny D.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.46348

Abstract

Spotcheek emperor L. rubrioperculatus lives in association with coral reefs, at a depth of 10-168 m. This species lives on sandy bottoms and along the outer reef slopes. The lemur fish L.rubriopercelatus is a protogynous hermaphrodite fish, there is a sex change from the female phase to the male phase. Research related to sea urchins in Likupang waters has never been carried out, even in general it is very limited in Indonesia. This study aims to analyze the length-weight relationship and reproductive aspects of spotcheek emperor at Napo Nain Likupang waters. Length-weight relationships are growth patterns and condition factors. Reproductive aspects are the level of gonadal maturity, gonadal maturity index, and fecundity. Fish samples were caught using two types of hand lines, hook number 10 with monofilament line number 90 and hook number 12 with monofilament line number 100. Sampling was carried out three times in May, June, and July 2022. The total samples were 80 individuals. consisting of 44 male individuals with a fork length range of 14.7-22.7 cm, 42 female individuals with a fork length range of 13.0-24.0 cm, and 2 individuals of transitional elephant fish, respectively 19.5 and 20.0 cm. Male length-weight relationship W= 0.2332L2.1642 (R2=0.7881) negative allometric growth pattern. Females W = 0.0868L2.505 (R2=0.9575) negative allometric growth pattern. The condition factor for male fish is 1.03 ± 0.22 and 0.99 ± 0.12 for females. The gonadal maturity was found in May for both males and females. Gonad Maturity Index for males ranged from 0.03-0.18 and for females 0.02-1.49. Fecundity ranged from 21.035-3.9497 with an average of 31.530 ± 5.929. The relationship between fecundity and length F=3152L0.7721 (R2=0.24340) Keywords: Growth pattern, Allometric, Gonad, Fecundity. Abstrak Ikan lencam L. rubrioperculatus hidup berasosiasi dengan terumbu karang, pada kedalaman 10-168 m. Spesies ini hidup di dasar berpasir dan di sepanjang lereng terumbu bagian luar. Ikan lencam L.rubriopercelatus adalah ikan hermaprodit protogini, terjadi perubahan kelamin dari fase betina ke fase jantan. Penelitian berkaitan ikan lencam di perairan Likupang belum pernah dilakukan, bahkan secara umum di Indonesia sangat terbatas. Penelitian ini bertujuan untuk menganalisis hubungan panjang-berat dan aspek reproduksi. Hubungan Panjang- berat meliputi pola pertumbuhan dan faktor kondisi di parairan Napo Nain Perairan Likupang. Aspek reproduksi meliputi tingkat kematangan gonad, indeks kematangan gonad dan fekunditas. Sampel ikan ditangkap dengan menggunakan dua jenis pancing ulur (hand line) mata pancing nomor 10 dengan tali monofilament nomor 90 dan mata pancing nomor 12 dengan tali monofilament nomor 100. Sampling dilakukan tiga kali pada bulan Mei, Juni dan Juli 2022. Jumlah sampel 80 individu terdiri atas terdiri dari 44 individu jantan dengan kisaran panjang garpu 14,7-22,7 cm, 42 individu betina dengan kisaran panjang garpu 13,0-24,0 cm, 2 individu ikan lencam transisi kelamin masing 19,5 dan 20,0 cm. Hubungan panjang-berat jantan W= 0,2332L2,1642 (R2=0,7881)pola pertumbuhan alometrik negatif. Betina W = 0,0868L2,505 (R2=0,9575) pola pertumbuhan alometrik negatif. Faktor kondisi jantan bernilai 1,03 ± 0,22 dan 0,99 ± 0,12 ikan betina. Tingkat kematangan gonad ikan yang siap memijah (TKG IV) ditemukan pad bulan Mei baik jantan maupun betina. Indeks Kematangan Gonad jantan berkisar antara 0,03-0,18 dan betina 0,02-1,49. Fekunditas berkisar antara 21.035-3.9497 dengan rata-rata 31.530 ± 5.929. Hubungan fekunditas dengan panjang F=3152L0,7721 (R2=0,24340) Kata kunci : Pola pertumbuhan, Allometrik, Gonad, Fekunditas
Structure of the Seagrass Community, In Poopoh Beach Waters, Tombariri District, Minahasa Regency, North Sulawesi Province Mamonto, Riswanto; Kondoy, Khiristin Ivone Fisye; Wantasen, Adnan S.; Kepel, Rene Charles; Pratasik, Silvester Beny; Menajang, Febry Susana Ivone
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.49599

Abstract

Seagrass are flowering plants (Angiosperms) that are fully adapted to life immersed in the sea. This plant consists of rhizomes, leaves and roots, (Rhizomes are stems that sink and creep horizontally, leave and flower, and grow roots). It is these rhizomes and roots that hold back the waves and currents on seagrass plants. The purpose of this study was to determine seagrass species, species density, relative density, species frequency, relative frequency, species closure, relative closure, important value index, diversity index, domination index and aquatic environmental conditions. There are 4 species of seagrass found in the waters of Poopoh Beach, namely, Thalassia hemprichii, Enhalus acoroides, Halodule pinifolia, Halophila ovalis. The number of stands of seagrass species in the study area ranged from 53-965 individuals, species density (17.67-321.67) individuals/m2, relative density (3.98-72.61%), frequency (0.045-0.90), relative frequency (3.57-71.42%), species closure (0.41-7.31), relative coverage (1.04-78.12%), seagrass importance value index in Poopoh Beach waters shows that Thalassia hemprchii has the highest importance value index among the other 3 seagrass species namely (212.85%), diversity index (0.97), species dominance index (0.11-0.44%/m²). Environmental conditions in the waters of Poopoh Beach, which has a temperature range of 38°C, a salinity of 35‰, has a substrate of sand, sand mixed with mud, muddy and coral fragments. Keywords: Poopoh Beach, Seagrass, Community Structure. Abstrak Lamun adalah tumbuhan berbunga (Angiospermae) yang sepenuhnya menyesuaikan diri dengan hidup terbenam dalam laut. Tumbuhan ini terdiri dari rhizoma, daun dan akar, (Rhizoma adalah batang yang terbenam dan merayap secara mendatar, berdaun dan berbunga, serta tumbuh akar). Rhizoma dan akar inilah yang menahan hempasan ombak dan arus pada tumbuhan lamun. Tujuan penelitian ini adalah untuk mengetahui jenis-jenis lamun,  kerapatan spesies, kerapatan relatif, frekuensi jenis, frekuensi relatif, penutupan jenis, penutupan relatif, indeks nilai penting, indeks keanekaragaman, indeks dominasi dan kondisi lingkungan perairan. Spesies lamun yang ditemukan diperairan Pantai Poopoh berjumlah 4 spesies yaitu, Thalassia hemprichii, Enhalus acoroides, Halodule pinifolia, Halophila ovalis. Jumlah tegakan spesies lamun dilokasi penelitian berkisar dari 53-965 individu, kerapatan spesies (17,67-321,67) individu/m2, kerapatan relatif (3,98-72,61%), frekuensi (0,045-0,90), frekuensi relatif (3,57-71,42%), penutupan jenis (0,41-7,31), penutupan relatif (1,04-78,12%), indeks nilai penting lamun diperairan Pantai Poopoh menunjukkan bahwa Thalassia hemprchii memiliki indeks nilai penting paling tinggi diantara ke 3 spesies lamun lainnya yakni (212,85%), indeks keanekaragaman (0,97), indeks dominasi spesies (0,11-0,44%/m²). Kondisi Lingkungan diperairan Pantai Poopoh yakni mamiliki kisaran suhu 28°C, salinitas 30‰, memiliki substrat pasir, pasir campur lumpur, berlumpur dan patahan karang. Kata Kunci: Pantai Poopoh, Lamun, Struktur Komunitas.
A Preliminary Studies of Fish Richness in the Tondano River Estuary Manado Bay Bataragoa, Nego E.; Pratasik, Silvester Benny; Menajang, Febry S. I.; Manginsela, Fransine B.; Dauhan, Dulce Maria; Tombi, Indra
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.50025

Abstract

The purpose of this study was to analyze the richness of fish species in the Tondano River estuary. Sampling was done for two months, in July and August 2022, new moon phase, during the low tide at 13.00-16.00 pm. Sampling used a 20-m beach seine with a cod-end part of 0.2-cm mesh and wings of 2-m height and 1.5-cm mesh. The beach seine was operated 10 times starting from the lowest tide at 13.00 pm to the tide condition at 16.00 pm. As a whole, 736 individuals were caught, consisting of 24 species and 17 families, 14 migrant species, and 10 resident species The number of fish varied from 1 to 445 individuals, in which Terapon jarbua (445 ind) was the most abundant, followed by Neovespicula depressifrons (70 ind). The dominant index (C) was 0.38 and the diversity index (H) was 1.65. The resident species were 120 individuals varying from 2 to 70 ind., and the most abundant was Neovespicula depressifrons (70 ind.). The dominant index (C) was 0.37 and the diversity index was 1.47.
Fish Community Structure Of The Bahu River Estuary Manado City: Sampe, Ayumi Angraini; Manu, Gaspar D.; Kambey, Alex D.; Pratasik, Silvester Benny; Rondonuwu, Arie B.; Rangan, Jety K.
Jurnal Ilmiah Platax Vol. 12 No. 2 (2024): ISSUE JULY-DECEMBER 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i2.55275

Abstract

Community structure is an ecological term to indicate what organisms are present in a given environment, in what numbers, and how they are interconnected, through ecological indices that reflect dominance, evenness, and richness traits. The estuary is the most downstream part of the river and is connected to the sea. The Bahu River estuary is a habitat for a variety of aquatic life, especially fish, where water quality conditions greatly affect the abundance, diversity, and dominance. The methods used in this study were general field survey methods and fishing methods using the "swept area" method (Sparre & Venema, 1998). Data collection was carried out based on the time of collection using beach seine gear. This research was conducted with several stages, namely fishing in the field which will become data, data analysis and discussion. The results of this study indicate the composition of fish species in the Bahu River Estuary there are 11 families, 11 genus, and 14 species with a total of 86 individuals. The results of data analysis of the highest relative abundance in the Bahu River Estuary were in June, namely the Ambassis urotaenia species with a value of 45.90%. The results showed the community structure in the Bahu River Estuary in June and July (H' = 1.53 - 1.64, J' = 0.64 - 0.79, D = 0.31 - 0.26). Keywords: Diversity; Evenness; Dominance; River Estuary.
Co-Authors Adnan Wantasen Agustiana Agustiana Alex D. Kambey Ali Djamhuri Ali, Fajri Nurul Anang Najamuddin Anneke V. Lohoo Antonius Rumengan Ari B. Rondonuwu Bataragoa, M.Sc, Dr. Ir. Nego E. Billy Th. Wagey Calvyn F. A. Sondak, Calvyn F. A. Celcius Waranmaselembun Choirul Anwar Darus S. Paransa Dauhan, Dulce Maria Deiske A. Sumilat Deslie RH Kumampung Elvy L. Ginting Erly Y Kaligis Erly Y. Kaligis, Erly Y. Firlianty Firlianty Firlianty Firlianty Firlianty, Firlianty Firlianty, Firlianty Fitje Losung Frans F. Tilaar Fransine B. Manginsela Gaspar D. Manu, Gaspar D. Gaspar Manu Ginting, Elvy Like Gustaf F. Mamangkey Hengky J. Sinjal, Hengky J. Inggrid M. F Akerina Janny D. Kusen John L. Tombokan Joshian NW Schaduw Joudy R.R. Sangari Juliaan Watung Kalesaran, Ockstan Y. Kondoy, Khiristin Ivone Fisye Lalamentik, Laurentius Th. X. Laura, Azhar Lawrence J. L. Lumingas Leleran, Andreas J. P. L. Lexy K Rarung Malurung, Yulianti - Mamonto, Riswanto Mangindaan, Remy Mantiri, Nicola R. K. Marselo R. Manzanaris Medy Ompi Medy Ompi Meiske S. Salaki Melani, Hellen Menajang, Febry S. I. Menajang, Febry Susana Ivone Moningkey, Ruddy D. Natalie D Rumampuk Nego E. Bataragoa Nego E. Bataragoa, Nego E. Novia A Jambo Pelle, Wilmy E. Purniasih, Ni Komang Pitri Ramadan, Febrian Rangan, Jety K. Rembet, Unstain Remy E. P Mangindaan Rene Charles Kepel, Rene Charles Robert A. Bara Rondonuwu, Arie B. Rondonuwu, Yonatan Y. Runtuwene, Heard C.C. Rustikasari, Irna Salaki, Meiske S. Sambali, Hariyani Sampe, Ayumi Angraini Sampouw, Mouren V. Samuel L. Opa Sangari, Joudy R.R Silvia A. Bangun Sinjal, Henky Stenly Wullur Suria Darwisito Suzanne L Undap Tidore, Fadli Tilaar, Ferdinand F. Tombi, Indra Unstain N. W. J. Rembet Veibe Warouw Wewengkang, Fabiola Wilmy Pelle Yuliana Anastasia Ngamel