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All Journal ACTA VETERINARIA INDONESIANA Jurnal Bios Logos JSME MIPA UNIMA Jurnal Sains dan Teknologi Jurnal Produksi Tanaman JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Penelitian Pendidikan IPA (JPPIPA) Dinamisia: Jurnal Pengabdian Kepada Masyarakat MATAPPA: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jambura Edu Biosfer Journal Fullerene Journal of Chemistry SOSCIED Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Vivabio : Jurnal Pengabdian Multidisiplin Prima Abdika: Jurnal Pengabdian Masyarakat BIOCHEPHY: Journal of Science Education Seminar Nasional Pengabdian Kepada Masyarakat Makara Journal of Science Indonesian Biodiversity Journal Jurnal Ilmiah Sains Indonesian Biodiversity Journal Servitium Smart Journal Jurnal Pengabdian Masyarakat Tekno J-CEKI PESHUM Advances in Tropical Biodiversity and Environmental Sciences
Emma M Moko
) Biology Department, Faculty Of Mathematics And Natural Sciences, Manado State University, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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MORPHOLOGY OF Patchouli OIL MICROCACULES (Pogostemon cablin Benth) WHICH IS ENKAPSULATED USING CHITOSAN Kleriyani Mayan; Arrijani Arrijani; Emma Moko
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v1i2.796

Abstract

Patchouli oil (Pogostemon calbin Benth) has economic benefits which are widely used as raw material for the pharmaceutical, perfume and food industries. Although widely used in various fields, patchouli oil or essential oils are generally young to evaporate, are susceptible to high temperatures and humidity, so we need a way / solution to overcome this, one way is encapsulation. Encapsulation is a technique to protect the core material which was originally liquid from becoming a solid / powder form and can protect the core material from losing its taste and aroma. Coating is a material that can mix with the core material, is not reactive to the core material and can form a layer around the core material. Chitosan is a good coating for essential oil encapsulation. This study aims to determine the particle size and microcapsule characteristics of patchouli oil (P.calbin Benth) using chitosan coating. The microcapsules were made using a freeze dryer with the freeze drying method. The dried samples were analyzed for particle size, morphological shape and content using SEM (Scanning Electron Microscopy). The results showed that the samples with a coating material composition of 1:2 were completely dry freeze drying. The average particle size <3.5 µm with a magnification of 10,000x with round morphology and various shapes. Analysis of the chemical content in the sample showed the similarity of the main compounds in patchouli essential oil, namely α-guaiene, azulene, α/β/ γ-patchoulene, seychellene, and α-himachalene and there was the addition of carbon (C) 76.07% and oxygen. (O) 16.46% originating from coating material
Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen Rahardiyan, Dino; Moko, Emma Mauren
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.44969

Abstract

 The abundance of lignocellulosic waste makes it a potential source for advanced biomaterials through various transformation processes. Lignocellulosic biomass transformation to advanced biomaterials involves enzymes from extracellular metabolites of microorganisms capable of hydrolyzing lignocellulose. This research was to molecularly screen fungi found in symbiotes of lichen endemic to trees growing in North Minahasa (North Sulawesi) with lignocellulolytic transformation enzymes. Molecular screening was conducted from identified fungi isolates based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Fungi isolates screening identified Trichoderma koningiopsis (isolate HZA8 and isolate HZA6), Penicillium sumatraense (strain CBS 127365 and strain CBS 130380), Trichoderma hamatum (isolate PAN12-45 and isolate PAN12-05), Aspergillus aculeatus (strain A1.9 18S),  Aspergillus aculeatus (isolate XSD-74), Trichoderma reesei (strain S2606 and isolate 5A14).  Molecular identification and BLAST homology of potentially lignocellulolytic fungi isolates rDNA indicated that isolate KB2 had close relationship with Trichoderma reesei at 100% degree of closeness and an index of cellulolytic activity of 1.19. While isolate KB3 appeared closely related to Aspergillus aculeatus at 99.83% degree of closeness and an index of cellulolytic activity of 1.57. Therefore, the potential of developing bioprocess industries in general and in particular is most probable.
KARAKTER KIMIA DAN PASTING PROPERTIES PATI JAGUNG (Zea mays) HASIL EKSTRAKSI MENGGUNAKAN NATRIUM METABISULFIT Moko, Emma Mauren; Rey, Feyne Jelita; Lawalata, Helen Joan; Rawung, Livana Dethris; Rahardiyan, Dino
Jurnal Sains dan Teknologi Pangan Vol 9, No 3 (2024):
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v9i3.48082

Abstract

Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh ekstraksi dengan perlakuan perendaman menggunakan natrium metabisulfit terhadap karakter pati jagung (Zea mays). Parameter yang diamati meliputi :kadar proksimat yaitu kadar abu, kadar protein, kadar karbohidrat, kadar lemak, dan kadar air), uji warna yaitu nilai L*, a* dan b*, karakter pasting properties yaitu peak viscosity, through viscosity, breakdoen viscosity, final viscosity, setback viscosity, peak time dan pasting temperature. Hasil penelitian menunjukan bahwa natrium metabisulfit memberikan pengaruh yang berbeda nyata terhadap kadar abu, protein, karbohidrat, lemak, air, warna, rendemen dan pasting properties.
DISEMINASI INOVASI DAN ALIH TEKNOLOGI PENGOLAHAN PATI JAGUNG BAGI MITRA SENTRUM AGRARIS LOTTA Moko, Emma Mauren; Rawung, Livana Dethris; Sitanayah, Lanny; Rampengan, Mercy Maggy Franky; Rahardiyan, Dino
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 30, No 2 (2024): APRIL-JUNI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v30i2.53191

Abstract

Sentrum Agraris Lotta (SAL) yang terletak di Desa Lotta Jaga II, Kecamatan Pineleng, Kabupaten Minahasa, Sulawesi Utara merupakan organisasi dengan sasaran strategis sebagai percontohan pertanian organik terpadu dengan tugas utamanya sebagai wadah edukasi bagi seluruh mitra kelompok tani dengan wilayah binaan Sulawesi Utara hingga Sulawesi Tengah, selain itu SAL merupakan organisasi yang turut membantu penanganan dan mitigasi bencana. Permasalahan utama SAL yaitu permasalahan pasca panen dan permasalahan pengembangan produk pertanian menjadi produk pasca panen bernilai jual tinggi serta permasalahan diversifikasi dan peningkatan added value berbagai komoditas SAL terutama komoditas jagung yang selama ini hanya dijual dalam bentuk pipilan biji jagung sebagai bahan baku pakan ternak tanpa adanya pengolahan  lanjut yang dapat meningkatkan added value dan diversifikasi produk.  Kegiatan diseminasi dan alih teknologi pengolahan pati jagung dilakukan bersama dengan mitra sasaran SAL dengan metode sosialisasi, diseminasi dan alih teknologi pengolahan pati menggunakan teknologi tepat guna mesin penggiling dan mesin press hidrolik yang melibatkan peran aktif dari peserta dan kelompok mitra dimana produk dari kegiatan ini berupa pati jagung serta dengan adanya kegiatan ini dapat meningkatkan pengetahuan dan ketrampilan mitra dalam pengolahan pati jagung dalam upaya diversifikasi dan peningkatan nilai tambah komoditas jagung.
Diseminasi dan Pemberdayaan Pengolahan Kecap Berbahan Baku Blondo Minyak Kelapa di Dusun Harapan, Desa Ilomata, Kecamatan Atinggola, Gorontalo Utara Arifin, Arifin; Fitriani, A. Nur; Angelia, Ika Okhtora; Mege, Revolson Alexius; Tanor, Meity Neltje; Moko, Emma Mauren; Mewengkang, Alfrina
Jurnal Aplikasi dan Inovasi Iptek Vol 5 No 1 (2023): Jurnal Aplikasi dan Inovasi Iptek No. 5 Vol. 1 Oktober, 2023
Publisher : Denpasar Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52232/jasintek.v5i1.122

Abstract

Kecamatan Atinggola merupakan salah satu kecamatan dalam wilayah Kabupaten Gorontalo Utara yang memiliki potensi penghasil kelapa dengan luas areal perkebunan 4,563 ha dan jumlah produksi kelapa 9,080 ton/ha/ tahun. Produk samping dari pengolahan minyak kelapa berupa ampas minyak kelapa atau blondo sekitar 60% dari total bahan baku santan yang digunakan dan hanya dimanfaatkan sebagai campuran pada bahan makanan tertentu seperti tambahan pada tumisan sayur atau sambal padahal blondo mengandung protein 24,22% yang dapat dimanfaatkan sebagai bahan baku pembuatan kecap manis. Kegiatan diseminasi dan pengolahan kecap manis berbahan baku blondo dilakukan bersama dengan mitra sasaran Kelompok Tani IKM F2-1 Dusun Harapan, Kecamatan Atinggola dengan metode sosialisasi, diseminasi dan pengolahan kecap yang melibatkan peran aktif dari peserta dan kelompok mitra dimana produk kegiatan berupa produk kecap manis berbahan baku blondo serta kegiatan ini dapat meningkatkan pengetahuan dan ketrampilan mitra dalam pengolahan kecap berbahan baku blondo
Pelatihan Pengolahan Kelapa dalam Menghasilkan Minyak Kelapa di Desa Imana, Kecamatan Atinggola, Gorontalo Utara Fitriani, A. Nur; Angelia, Ika Okhtora; Arifin, Arifin; Mege, Revolson Alexius; Moko, Emma Mauren; Mewengkang, Alfrina
Jurnal Aplikasi dan Inovasi Iptek Vol 5 No 2 (2024): Jurnal Aplikasi dan Inovasi Iptek No. 5 Vol. 2 April, 2024
Publisher : Denpasar Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52232/jasintek.v5i2.138

Abstract

Desa Imana terletak di Kab.Gorontalo Utara memiliki tanaman kelapa yang sangat melimpah dan menjadi salah satu sumber penghasilan masyarakat disekitarnya. Tujuan dari kegiatan ini memberikan pelatihan kepada warga dalam pengolahan kelapa guna menghasilkan minyak kelapa secara tradisional dan berkualitas pada desa Imana kecematan Atinggola, Kab Gorontalo Utara. IKM Mikas melakukan proses pembuatan minyak kelapa secara tradisional dengan metode cara basah berbeda dari pembuatan minyak pada umumnya, adapun tahapan proses pembuatan melalui sortasi bahan baku, pengepresan santan, pemeraman santan selama 24 jam, ekstraksi santan melalui pemanasan hingga diperoleh minyak murni kelapa. Berdasarkan studi literatur minyak kelapa tradisional mengandung 5 jenis asam lemak yaitu asam laurat (44,83%), asam miristat (22,83%), asam palmitat (12,82%), asam oleat (11,68%), dan asam stearat (3,90%). Minyak kelapa yang dihasilkan IKM Mikas dari segi aroma dari sisi aroma yang jauh lebih harum dibanding dengan produk-produk pabrikan. Menurut Bapak Supanto Patingki yang merupakan Ketua IKM Mikas, setiap bulannya IKM Mikas memproduksi minyak kelapa kampung sebanyak 4 kali (sepekan sekali) Setiap produksi menghasilkan 100 botol
SUCCESSION OF LACTIC ACID BACTERIA ON CANDIED PAKOBA FRUIT (Syzygium luzonense) Kembuan, Patricia Pericilia; Mokosuli, Yeremia Samuel; Lawalata, Helen J.; Moko, Emma; Roring, Verawati I. Y
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.878

Abstract

Pakoba fruit is often made into sweets, salad, pickles because it has a very sour taste and is a habitat for lactic acid bacteria. The purpose of this study was to determine the characteristics of lactic acid bacteria succession during the fermentation process of candied pakoba fruit. This research is an explorative research. The number of microbes was observed starting from day 0, 1, 3 and 7. Based on the results of identification data, 12 bacterial isolates were obtained in pakoba fruit fermentation. The isolates obtained were then macroscopically identified and microscopically identified. 12 isolates were round, 12 isolates had enpitire edges, 12 isolates had raised elevations, 12 isolates had covex colony surfaces, 10 isolates were white and 2 isolates were cream-colored. Of the 12 isolates, it was found that the bacteria that grew in the fermentation of candied pakoba fruit after profile matching was suspected of the genus Lactobacilus sp. and Leucunostoc.
APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

Abstract

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.
APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

Abstract

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.
Observations on the Biodegration by Solid State Fermentation of the Giant Swamp Taro (Cyrtosperma merkusii) Biomass by two endolichenic fungi isolates Helena, Maria; Lawalata, Helen Joan; Rahardiyan, Dino; Rawung, Livana Dethris; Moko, Emma Mauren
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Giant swamp taro (GST/Cyrtosperma merkusii), a local North Sulawesi coastal plant, is underutilized but has high po-tential for cultivation as a paludiculture plant on rewetted peatland restorations. GST has high starch content and is currently being explored for potential use. This study explored the potential of using the biomass pulp yielded as resi-dues of the starch extraction process. Endolichenic fungi (Trichoderma reesei and Aspergillus aculeatus) isolated from local lichens, were inoculated on the GST biomass in a solid state fermentation (SSF). Findings of this study indicate that both isolates were effective in degrading the lignocellulosic biomass of GST over an incubation period of 28 days, focusing on cellulose as the targeted polysaccharide. Although T. reesei requires a more adjusted carbon source than A. aculeatus to effectively process the biomass, the effectiveness of the bioprocess after adjustment matched that of A. aculeatus. When hemicellulose is targeted, other strategies must be used to prevent destruction of hemicellulose during the process. This phenomenon should be explored in further studies. These findings indicate the potential of industrial-izing cellulose-based products from GST using local North Sulawesi fungal species abundantly occurring in local eco-systems.
Co-Authors A. Nurfitriani Alberto Y T Allo Alfonds Andrew Maramis Alfrina Mewengkang Anatje Lihiang Anatje Lihiang Anita Costanci Christine Tengker Arifin Arifin Arista, Melti Arrijani - Aser Yalindua Christny Ferdina Evie Rompas Crescentia Mishi Juniorita Ngangi Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Dino Rahardiyan Dino Rahardiyan Dino Rahardiyan Djarang, Rievo H Esrah Anna Adeleid Agimat Fanny Nella Nanlohy Ferencia Esananda Rattu Ferni Margo Tumbel Ferry Wantouw Ferry Wantouw Fitriani, A. Nur Gedoan, Sukmarayu Pieter Gedoan, Sukmarayu Piter Grace Jenny Soputan Helen Joan Lawalata Helena, Maria Herry Maurits Sumampouw Ika Okhtora Angelia Iren Natalia Simanjuntak Iriani Setyawati Jabes Wolter Kanter Jacson Saruan Jantje Ngangi Jumiaty Christianity Manoppo Junaidy Budi Sanger Kabenaran, Gethria F. Karuntu, Rilya Patricia Esther Kawatak, Steven Y. Kawengian, Priska Rosalina Kembuan, Patricia Pericilia Kleriyani Mayan Lanny Sitanayah Lensehe, Febby Anggini Leu, Paulus L. Livana Dethris Rawung Lohonauman, Charlie A. P. Maliangkay, Hendra Pratama Manampiring, Nonny Mario Poluakan Meity Nelltje Tanor Melany Piri Michelle Chantika Umboh Mokosuli Yermia Samuel Mokosuli, Yeremia Samuel Ngangi, Crescentia M.J Nonny Manampiring Ogi, Nova LIM Orbanus Naharia Orbanus Naharia Patandean, Selvrining Paulus Boli Paulus L. Leu Pinaria, Yeanly Wuena Posumah, Danny Christian Purba, Gandi YS Rahardiyan, Dino Rampengan, Johan A. Rampengan, Mercy Maggy Franky Rayer, Debby J J Regina R. Butarbutar Revelson A. Mege Revolson Mege Rey, Feyne Jelita Rorimpandey, Vianney Indah Roring, Verawati I. Y Rudi Alexander Repi Sakul, Ernest Hanny Saruan, Jacson Sukmarayu P. Gedoan Tedang, Bybyani Teresa Maria Wangkar Timbuleng, Nobel Tuelah, Syela Nathasya Veiren Wangkanusa Verawati I Y Roring wa, Johanna Zusye Wantania, Johanna Zusye Zulviana Y. Sidabariba Zusje Warouw