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Journal : Jurnal Kedokteran Hewan

KORELASI FOLIKEL DOMINAN AKIBAT PENYUNTIKAN HORMON PREGNANT MARE SERUM GONADOTROPIN (PMSG) DENGAN PENINGKATAN RESPONS BERAHI PADA KAMBING KACANG Andriyanto A; Amrozi A; Min Rahminiwati; Arief Boediono; Wasmen Manalu
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.65 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2779

Abstract

Penelitian ini bertujuan mengetahui pengaruh penyuntikan pregnant mare serum gonadotropin (PMSG) terhadap peningkatan respons berahi kambing kacang. Sebanyak 20 ekor kambing kacang betina dewasa kelamin dibagi secara acak ke dalam 5 perlakuan dosis penyuntikan PMSG, yaitu 0,0 (K1); 1,0 (K2); 7,5 (K3); 15,0 (K4); dan 20,0 (K5) IU/kg bobot badan dan diulang 4 kali. Hasil penelitian menunjukkan bahwa pada K3, K4, dan K5 terjadi peningkatan jumlah folikel dominan, total diameter folikel dominan, total volume folikel dominan, dan konsentrasi estrogen dalam serum darah (P0,05), serta menunjukkan gejala berahi yang lebih jelas dibandingkan K1. Konsentrasi estrogen dalam serum darah memiliki korelasi positif dengan jumlah folikel dominan, total diameter folikel dominan, total volume folikel dominan, onset berahi, durasi berahi, perubahan vulva menjadi merah, kebengkakan vulva, peningkatan respons betina terhadap pejantan, dan pergerakan ekor pada saat berahiatau fleging berturut-turut sebesar 53,10; 82,60; 48,80; 53,0; 45,50; 39.40; 32,60; 32,60; dan 80,20% dengan pola kuadratik. Dari hasil penelitian disimpulkan bahwa penyuntikan PMSG pada dosis 7,5; 15,0; dan 20,0 IU/kg bobot badan mampu meningkatkan konsentrasi estrogen dan respons berahi kambing kacang.
DINAMIKA OVARIUM SAPI ENDOMETRITIS YANG DITERAPI DENGAN GENTAMICINE, FLUMEQUINE DAN ANALOG PROSTAGLANDIN F2 ALPHA (PGF2α) SECARA INTRA UTERUS Juli Melia; Amrozi A; Ligaya ITA TumbelakaLigaya
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.083 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2628

Abstract

Penelitian ini bertujuan mengetahui efektivitas pengobatan endometritis dengan menggunakan kombinasi antibiotik (gentamicine, flumequine) dan analog prostaglandin F2 alfa (PGF2α) berdasarkan pengamatan dinamika ovarium dengan metode ultrasonografi (USG). Enam ekor sapi endometritis dibagi dalam 2 kelompok perlakuan. Kelompok I (K1, n= 3) diterapi dengan 250 mg Gentamicine, 250 mg Flumequine, dan 12,5 mg PGF2α secara intra-uterus. Kelompok II (K2, n= 3) diterapi menggunakan antibiotik dengan dosis dan cara pemberian yang sama seperti pada Kelompok I. Hasil pengamatan terhadap sapi-sapi endometritis K1 dan K2 memperlihatkan rataan panjang siklus estrus selama 18 hari. Hasil analisis statistik menunjukkan tidak ada perbedaan yang nyata (P>0,05) terhadap kelas folikel pada kedua kelompok perlakuan. Folikel besar (DF) dan folikel besar kedua (SF) pada ke-3 gelombang folikel yang muncul tidak memperlihatkan perbedaan yang nyata antara K1 dan K2 (P>0,05), tetapi terdapat perbedaan yang nyata (P
PERSENTASE KEBUNTINGAN KEMBAR DAN ENUKLEASI VESIKEL EMBRIO DENGAN PANDUAN ULTRASOUND PADA KUDA Amrozi A; Ligaya ITA Tumbelaka
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.785 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1258

Abstract

Penelitian ini bertujuan mengetahui persentase kebuntingan kembar pada kuda di Pulau Jawa dan Madura dan menguji tingkat keberhasilan enukleasi vesikel embrio dengan panduan ultrasound. Sebanyak 354 induk kuda dikawinkan secara alami dengan kebuntingan mencapai 57,6% (204 ekor) dan ditemukan 11 kebuntingan kembar (5,4%). Kebuntingan kembar dengan 2 vesikel embrio pada 10 induk dan 3 vesikel embrio pada satu induk. Pengurangan embrio dengan memberikan tekanan probe terhadap vesikel embrio berpanduan gambaran ultrasound terhadap 6 ekor kuda bunting kembar berhasil pada satu ekor induk kuda (16,6%) dan melahirkan satu anak. Semua kontrol kebuntingan kembar pada 5 induk kuda mengalami abortus pada usia kebuntingan 7-9 bulan. _____________________________________________________________________________________________________________________
SINKRONISASI ESTRUS DAN PENGAMATAN ULTRASONOGRAFI PEMERIKSAAN KEBUNTINGAN DINI PADA DOMBA GARUT (Ovis aries) SEBAGAI STANDAR PENENTUAN UMUR KEBUNTINGAN Amrozi a; Bagus Setiawan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.786 KB) | DOI: 10.21157/j.ked.hewan.v5i2.364

Abstract

Penelitian ini dilakukan untuk mengamati sinkronisasi estrus dan menentukan diagnosis kebuntingan dini pada domba garut (n=3) menggunakan ultrasonografi transrektal. Domba disinkronisasi dengan menggunakan PGF pada fase luteal. Onset dan durasi estrus diamati dengan menggunakan jantan pengusik. Kebuntingan 2a ditentukan dengan tampilan isoechogenic yang dikelilingi oleh tampilan hypoechogenic. Rata-rata onset estrus adalah 35±28,7 jam dan rata-rata lama estrus adalah 33±13,6 jam. Kebuntingan dini terdeteksi pada hari ke-22 (22,3±0,6 hari). Perkembangan fetus diikuti dengan peningkatan diameter dan ketebalan uterus. Diameter uterus meningkat dari hari ke-22 (1,8±0,7 cm) hingga hari ke-42 (5,6±1,1 cm), dan tebal uterus meningkat dari hari ke-22 (0,8±0,1 cm) hingga hari ke-42 (2,1±0,5 cm). Plasentom muncul pada kebuntingan hari ke-34 (0,8±0,2 cm) dan menunjukkan pola perkembangan yang terus meningkat secara signifikan sampai hari ke-56 (2,7±0,5 cm; P0,05). Diameter kotiledon hari ke-34 sekitar 0,8±0,2 cm hingga hari ke-56 (2,7±0,5 cm) dan hari ke-77 (3,3±0,4 cm). Dapat disimpulkan bahwa diagnosis positif dari kebuntingan pada domba garut terlihat pada hari ke-12 dan fetus dapat diamati pada hari ke-22.
THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN Ariantie, Oriza Savitri; Amrozi, Amrozi; Yusuf, Tuty Laswardi; Rochman, Nurul Taufiqu; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.19669

Abstract

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.000.11 and -9.220.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90168.86 nm with PI value 0.340.04 and ZP-28.71.24 mV. The particle size of freeze-dried egg yolk powder was 1296.0086.73 nm, with PI value 0.700.04 and ZP-34.50.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.116.06% for 168 hours, compared to FEY-Tris (51.256.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.476.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.
PREVALENCE OF MARES GRANULOSA THECA CELL TUMOR (GTCT) IN INDONESIA Amrozi, Amrozi; Melia, Juli; Parwati, Luci; Tumbelaka, Ligaya I.T.A.
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.12797

Abstract

This study was conducted to determine the prevalence rate of mares GTCT in Indonesia. Ultrasound examination of ovaries were performed on mares in Java and Madura Island of Indonesia. Thirteen mares had been affected by GTCT from 2913 number of examinations. The prevalence rate of GTCT during year 2006 and 2007 were 4.71% and 5.56%, respectively followed by a decrease in prevalence rate of GTCT during 2008-2014. The reproductive cycle in a mare with GTCT had returned to normal 6 months after ovariectomy.
ULTRASOUND IMAGING OF POSTPARTUM UTERINE INVOLUTION AND OVARIUM DYNAMIC IN ONGOLE CROSSBREED COWS Sukareksi, Hazar; Amrozi, Amrozi; Tumbelaka, Ligaya ITA
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.13697

Abstract

This study aimed to determine ultrasound of uterine involution and postpartum ovarian dynamics on Ongole Crossbreed Heifer (PO) associated with postpartum estrus signs. This study used 6 PO cows which were divided into primiparous group and pluripara group. The observation of uterine involution and ovarian dynamics was started from the first day postpartum using ultrasonography (USG) with a linear probe rectally, while the reproductive organs images were recorded every 2 days. The results showed that the time needed for all reproductive organs of 3 primiparous groups to complete the uterine involution after parturition was 37.331.15 days when the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine were 3.140.00 cm, 2.860.00 cm, 3.200.06 cm, 4.660.01 cm, and 4.660.01 cm, respectively. The time needed for all reproductive organs of 3 pluripara groups to complete uterine involution postpartum was 38.671.15 days with the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine 3.180.00 cm, 2.700.02 cm, 3.080.02 cm, 4.420.01 cm, and 4.420.01 cm, respectively. The average times of the first and second ovulation of primiparous cattle were 27.671.15 and 47.671.15 days postpartum, whereas in pluripara group was 28.331.15 and 48.331.15 days postpartum. At first ovulation all cows were not accompanied by signs of estrus, while at the second ovulation 2 primiparous cows and 1 pluripara cow showed less obvious signs of estrus, 1 primiparous cow and 1 pluripara cow showed signs of medium estrus, and 1 pluripara cow showed clear estrus signs.
APPLICATION OF ESTRUS SYNCHRONIZATION USING PGF2 AND OVULATION SYNCHRONIZATION USING hCG FOR ARTIFICIAL INSEMINATION OPTIMIZATION ON ONGOLE (PO) BREED CATTLE Mutmainnah, Mutmainnah; Amrozi, Amrozi; Tumbelaka, Ligaya ITA
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.5940

Abstract

This study aimed to determine the pregnancy percentage of Ongole (PO) breed cattle by estrus synchronization and ovulation synchronization. This study used 22 cattle that were divided into three groups: Estrus synchronized cattle (K1, n= 5); ovulation synchronized heifers using ovsynch (K2, n= 6); and ovulation synchronized cow using ovsynch (K3, n= 11). Parameters measured were diameter of corpus luteum (CL) in estrus synchronization, follicular diameter upon synchronization and artificial insemination (AI), and percentage of pregnant cattle. Data obtained were statistically analyzed using analysis of variance followed by Duncan test. Results showed no significant differences (P0.05) of CL diameter at the time of estrus synchronization in all groups of cattle with an average of 16.633.79 mm. The CL diameter at the time of estrus synchronization was not significantly different among groups, with an average of 8.80 2.07 mm. Diameter of follicles during ovulation synchronization was also not significantly different among groups. The average diameter of follicles was 9.012.05 mm. Diameter of follicles at the time of estrus and ovulation synchronization was not significantly different among groups with an average diameter of follicles of 10.942.10 mm. The pregnancy percentage of K1, K2, and K3 were 60%, 16%, and 36%, respectively. There was no correlation between the diameters of follicles during estrus with the pregnancy percentage. Estrus synchronization produced higher pregnancy rate than ovulation synchronization in cow or heifers.
ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle) Melia, Juli; Agil, Muhammad; Supriatna, Iman; Amrozi, Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5026

Abstract

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Massons trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mares reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mares reproductive tract from labia to apex cornua was 48.001.00 cm. Weight of Gayo mares left ovary was 19.077.70 g and the right was 24.430.83 g. Histologically, there was no difference between Gayo mares structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.430.10 cm in horses with estrous cycle of 21 days and 6.300.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.
GAMBARAN ULTRASONOGRAFI OVARIUM KAMBING KACANG YANG DISINKRONISASI DENGAN HORMON PROSTAGLANDIN F2 ALFA (PGF2) DOSIS TUNGGAL S, Santoso; A, Amrozi; Purwatara, Bambang; H, Herdis
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.3077

Abstract

Penelitian ini bertujuan mempelajari gambaran ultrasonografi (USG) ovarium kambing kacang setelah penyuntikan hormon prostaglandin. Penelitian ini menggunakan enam ekor kambing kacang berumur 2-3 tahun, pernah melahirkan, dan bersiklus reproduksi normal. Pengamatan ovarium dilakukan dengan menggunakan USG selama 7 hari sebelum penyuntikan hormon prostaglandin sampai dengan ovulasi. Pengamatan ovarium dilakukan setiap hari dan diintensifkan setiap 12 jam menjelang ovulasi. Pengamatan ovarium terdiri atas folikel dan korpus luteum (CL). Pengamatan visualisasi respons berahi dilakukan sebelum dan setelah penyuntikan hormon prostaglandin. Hasil penelitian menunjukkan bahwa ovarium memperlihatkan dinamika folikel dan CL. Dinamika folikel dikarakteristikkan dengan gelombang folikel. Interval antara penyuntikan hormon prostaglandin sampai dengan ovulasi ialah 52,86,6 jam dengan nilai rataan diameter folikel ovulasi 5,70,7 mm. Visualisasi respons berahi pada kambing kacang tidak bermakna baik sebelum dan setelah penyuntikan hormon prostaglandin.
Co-Authors . Aryogi A. Boediono A. Winarto Ade Ocktaviani Ade Ocktaviani, Ade Adi Winarto Afiqah binti Abd Latif Agik Suprayogi Agus Setiyono Akbar Wijaya Putra Purnama Andriani Andriani Andriyanto . Andriyanto A Andriyanto Andriyanto Anita Esfandiari Arief Boediono Arifianto, Dinar Arman Sayuti Arman Sayuti Asrori Asrori Aulia Andi Mustika Azery bin Kamiring B Purwantara B Purwantara B. Purwantara Bagus Setiawan Bagus Setiawan Bambang Purwantara Bambang Purwatara Bambang Purwatara, Bambang Bambang Sigit Widodo Bayu Febram Prasetyo Bondan Achmadi Bondan Achmadi Bondan Achmadi Bondan Achmadi, Bondan Budianto Panjaitan D Sajuthi Darsono Darsono Dedi Rahmat Setiadi Diah Nugrahani Pristihadi Dian Masyitha E. Y. Yessa Edelina Sinaga Elma Yuliani Yessa Erly R Adistya Erly Rizka Adistya Erly Rizka Adistya Fiqhi Alfiansyah Hamdika Yendri Hazar Sukareksi Hazar Sukareksi, Hazar Herdis . Herdis H Herdis H Herdis Herdis Hidayati Mukarromah Hidayati Mukarromah Ho Kin Wai I Supriatna I wayan Teguh Wibawan I. Wientarsih IETJE WIENTARSIH Ietje Wientarsih Iga Mahardi Iga Mahardi Iman Supriatna Juli Melia Juli Melia K. B. Putro Kamiliyah, Kamiliyah Kiki Amalia Rama koekoeh santoso Krido Brahmo Putro Kudang Boro Seminar Langgeng Priyanto Leo Sapelani Soinbala Ligaya I. T. A. Tumbelaka Ligaya ITA Tumbelaka Luci Parwati M Agus Setiadi M Ibnu Satria M. F. Ulum M. Ibnu Satria Maharani Maharani, Maharani Mokhamad Fakhrul Ulum, Mokhamad Muchidin Noordin MUHAMMAD AGIL Muhammad Danang Eko Yulianto Muhammad Imron Muhammad Imron Muhammad Imron Mutmainnah Mutmainnah Mutmainnah Mutmainnah Ni Wayan Kurniani Karja nisfu bayu kurniadi Nugroho Sampurno Nur Anisa BS Nurul Taufiqu Rochman Nurul Taufiqu Rochman Oriza Savitri Ariantie Oriza Savitri Ariantie P Situmorang Parwati, Luci R I Afiriantini Rachmat Herman Rahminiwati, Min Rastina Rastina Riasari Gail Sianturi Ridi Arif Rivangga Yuda Hendika Roslizawaty r Roslizawaty r Roslizawaty Roslizawaty Rusdin - Santoso . Santoso S Santoso S Santoso Santoso Sari Yanti Hayanti Satya Gunawan Setiadi, Hirawan Siti Dwi Rahmah Ayumi Solly Aryza Srihadi Agungpriyono Sriyanto - Sugganya a/p Ravi Syafruddin S Syafruddin s T L Yusuf Tukiran TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf W. Manalu Wasmen Manalu Yessa, Elma Yuliani Yudha Fahrimal Yudha Fahrimal Yudi Eka Satria