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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Pengolahan Hasil Perikanan Indonesia Media Veteriner Jurnal Sain Veteriner Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Majalah Kedokteran Bandung Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Indonesian Journal of Pharmaceutical Science and Technology agriTECH Warta IHP (Warta Industri Hasil Pertanian) INDONESIAN JOURNAL OF PHARMACY Indonesian Food Science and TechnologyJournal JURNAL PANGAN Agroteknika Jurnal Mutu Pangan : Indonesian Journal of Food Quality Canrea Journal: Food Technology, Nutritions, and Culinary Journal Narra J Jurnal Kedokteran Hewan Molekul: Jurnal Ilmiah Kimia Jurnal Kefarmasian Indonesia Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)

Tutik Wresdiyati

IPB University

Author-ID : 209018

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; I Ketut Mudite Adnyane; Renny Candra Prasetyawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2848

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.

Deteksi Secara Imunohistokimia Oxygen-Free Radical Scavenger-Copper, Zinc-Superoxide Dismutase (Cu,Zn-SOD) pada Hati Tikus di Bawah Kondisi Stress Tutik Wresdiyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.2853

Copper,zinc-superoxide dismutase (Cu,Zn-SOD) yang merupakan salah satu oxygen-free radical scavenger telah dideteksi secara imunohistokimia pada hati tikus di bawah kondisi stres. Sejumlah empat puluh lima ekor tikus jantan galur Wistar telah digunakan pada penelitian ini. Hewan percobaan tersebut dikelompokkan menjadi tiga kelompok, yaitu : (1) kelompok kontrol, (2) kelompok dengan perlakuan stres selama 3 hari, dan (3) kelompok dengan perlakuan stres selama 5 hari. Stres yang diberikan adalah puasa, dengan pemberian air minum secara ad libitum. Stres yang diberikan menimbulkan keadaan histopatologis, peradangan dan nekrosis pada jaringan hati kelompok perlakuan tersebut. Secara imunohistokimia menunjukkan adanya penurunan kandungan Cu,Zn-SOD pada jaringan hati kelompok perlakuan dibandingkan pada kelompok kontrol. Penurunan kandungan Cu,Zn-SOD tersebut lebih hebat pada kelompok perlakuan selama 5 hari dibandingkan dengan kelompok perlakuan selama 3 hari. Hasil penelitian ini menunjukkan bahwa kondisi stres kemungkinan dapat meningkatkan terbentuknya oxygen-free radical yang kemudian merusak jaringan hati dan menurunkan kandungan Cu,Zn-SOD.

Profil Imunohistokimia Antioksidan Copper, Zinc-Superoxide Dismutase (Cu, Zn, -SOD) pada Ginjal Tikus Perinatal dan Neonatal Tutik Wresdiyati; I Ketut Mudite Adnyane; Silvia Arin Prabandari; Sofiawati Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2913

The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.

ANALISIS KANDUNGAN NUTRISI DAGING DAN TEPUNG TERIPANG PASIR (Holothuria scabra J.) SEGAR Rahman Karnila; Made Astawan; Sukarno Sukarno; Tutik Wresdiyati
Berkala Perikanan Terubuk Vol 39, No 2 (2011): Juli 2011
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Sea cucumber (Holothuria scabra J.) is one of fishery commodities whichcontain high nutritional and economic value. This study aims were 1) thephysical characteristics of sea cucumber, 2) nutrient content (proximate) seacucumbers fresh meat, and 3) the yield and nutrient content (proximate) seacucumber powdery fresh meat. The study was conducted in four stages of theexperiment: 1) Preparation of meat of sea cucumber, 2) analysis of nutrientcontent (proximate) sea cucumber fresh meat, 3) The making of powder of seacucumber meat , and 4) analysis of yield and nutrient content (proximate) seacucumber meat powder. Results showed the proportion between the parts of thebody meat: offal and gonads: the skin: water and dirt were 4:3:2:1 (w / w). Freshsea cucumber meat has a high nutrient content that is 87.03% water, 9.94%protein, 0.54% fat, 1.86% ash, and 0.64%. carbohydrate. The average yieldproduced approximately 10.16%. While the nutrient content (proximate) powderysea cucumber meat that is 9.13% water, 61.31% protein, 3.68% fat, 12.52% ash,and 13.36% carbohydrate.

The Effect of VCO Processing Method on Blood Glucose, Cholesterol and Pancreatic Profile of Diabetic Mellitus Rats (Sprague Dawley) Dadang Supriatna; Amilia Dayatri Uray; Made Astawan; Deddy Muchtadi; Tutik Wresdiyati
Warta Industri Hasil Pertanian Vol 35, No 2 (2018)
Publisher : Balai Besar Industri Agro

Virgin Coconut Oil (VCO) can be produced by different methods related to health function. The objective was to study the efficacy of VCO processing method in reducing blood glucose level, cholesterol profile and pancreatic tissue profile of diabetic mellitus (DM) Sprague dawley rats. Four rat groups from five rat groups were injected using alloxan to induce the diabetic condition, while the rest was negative control. VCO-A produced without heat and VCO-B produced with controlled-heat treatment and Coconut Cooking Oil (RBD-CNO) produced with the application of severe heating and chemical (MG) were fed respectively to three rat groups, while the other two rat groups were fed by water and used as negative control (healthy rats) and positive control (DM rats). During 28 days observation, all of the rats blood glucose level were assessed, and at the end of the observation day the rats were terminated to assess the cholesterol and pancreatic tissues profile. VCO-A significantly positive reduced blood glucose level than the others. VCO did not influence DM rats cholesterol profile and all VCO inhibits the rate of pancreatic β cells damage. On 28 days observation all VCO treatments suppressed blood glucose level of DM rats.

HYPOGLYCEMIC ACTIVITY OF SOME INDONESIAN RICE VARIETIES AND THEIR PHYSICOCHEMICAL PROPERTIES Sri Widowati; Made Astawan; Deddy Muchtadi; Tutik Wresdiyati
Indonesian Journal of Agricultural Science Vol 7, No 2 (2006): October 2006
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v7n2.2006.p57-66

Diabetes mellitus is a spectrum of inherited and acquired disorders characterized by elevating blood glucose levels. Diabetes is an abnormal carbohydrate metabolism, therefore, diet therapy for diabetics plays a key role in the management of the disease. Most Indonesian people consume rice as source of energy and protein. Generally, diabetics consume very limited rice because they believe that rice is the one of hyperglycemic food, even though different rice varieties have large range of glycemic index. The study aimed to evaluate hypoglycemic properties by using rat assay and chemical characteristics of 10 Indonesian rice varieties, i.e. Pandan Wangi, Rojolele, Bengawan Solo, Cenana Bali, Memberamo, Celebes, Ciherang, Batang Piaman, Cisokan, and Lusi. Taj Mahal, an herbal ponni imported rice, was used as a comparison. Male Sprague Dawley rats (150- 200 g body weight) were used for hypoglycemic assay. The rats were fasted overnight before the blood glucose was measured in the morning. The rats were then feed with 4.5 g rice per kg body weight by oral administration, followed by 1 ml of 10% glucose solution in the next 30 minutes. The blood glucose was measured for the next 30, 60, 90, and 120 minutes. Changes in blood glucose concentrations (mg dl-1) before and after the oral administrations were calculated for each rice variety tested. Results showed that Cisokan and Batang Piaman were categorized as low glycemic responses and Ciherang as high glycemic response, while the other varieties (Memberamo, Cenana Bali, Lusi, Bengawan Solo, Pandan Wangi, Celebes, and Rojo Lele) showed moderate glycemic responses. As the best hypoglycemic activity, Cisokan contained high amylose (27.6%), fat (0.87%), total dietary fiber (6.24%), resistant starch (2.02%), and lowest starch digestibility (52.2%), which are ideal for diabetic’s consumption. Ciherang as the worst hypoglycemic activity had low resistant starch (1.78%), low total dietary fiber (4.52%), and medium amylose (23.0%). This study implies that Cisokan variety is suitable for diabetic’s consumption.

Effects of brown algae extract to function of aorta endothelium cell in diabetic rats Muhamad Firdaus; Made Astawan; Deddy Muchtadi; Tutik Wresdiyati; Sarwono Waspadji; Setyawati S. K.
Indonesian Journal of Pharmacy Vol 21 No 3, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Macro and microvascular disease are currently the principal causes of morbidity and mortality in diabetes mellitus patients. Loss of the modulatory role of the endothelium may be a critical and initiating factor in the development of diabetic vascular disease. Polyphenol is known behave protective active compound to function endothelium cell. Polyphenol that contained in brown algae was phlorotannin. The aim of the present study was to investigate the effects of S. echinocarpum extract on endothelial dysfunction in aorta of streptozotocin diabetic rats. This experiment was conducted by complete randomized design in 2 months old of male albino Sprague Dawley strain of rats, 125-150 grams body weight. The rats divided in fivegroups, each group consist of 6 rats. First group: normal which is given 1 mL of extract vehicle orally; second group: diabetic rats which is given 1 mL of extract vehicle orally; third group: diabetic rats which is treated extract 150 mg/kg BW dose orally; fourth group: diabetic rats which is treated extract 300 mg/kg BW dose orally; and fifth group: diabetic rats which is treated extract 450 mg/kg BW orally. Diabetic rats prepared by injection of 45 mg/kg streptozotocin intraperitoneally. Extract was given to diabetic rats during 90 days. The result showed that brown algae extract rearrange endothelium cell function of diabetic rats by increasing relaxation, releasing of EDRF and decreasing of MDA. Based on these observations, it was concluded that S. echinocarpum extract recovers the endothelial dysfunction associated with diabetes. This effect appears to be due to its antioxidant properties.Key words: diabetes, EDRF, endothelial dysfunction, MDA, S. Echinocarpum

Evaluasi Mutu Protein Tepung Tempe dan Tepung Kedelai Rebus pada Tikus Percobaan Made Astawan; Tutik Wresdiyati; Armando M Saragih
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 2 No. 1 (2015): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar

Soybean is a strategic commodity in Indonesia and it is the main raw material for food processing such as tempe, tofu,and soy sauce. Tempe is a traditional food that has been known in Indonesia, which is made by fermentation process using Rhizopus sp. The main weakness of fresh tempe is the short shelf- life at room temperature, only last two days. Processing tempe into tempe our is one of efforts to extend the shelf life. This research aimes to evaluate protein nutritional quality of tempe our and soybean our, compared to casein as a standard by using rats as animal model. Weight gain of each group of rats was increased during 28 days of experiment. The protein nutritional quality of tempe our was signi cantly higher (p<0.05) than that of boiled soy our in the value of feed convertion efficiency and true protein digestibility; highly significantly higher (p<0.01) in the value of protein efficiency ratio and net protein ratio; and not significantly different (p>0.05) in the value of biological value and net protein utulization. From the results of protein nutritional quality evaluation, it could be concluded that tempe our had better nutritional quality than boliled soy our, and even better than casein.

Evaluasi Nilai Gizi Protein Tepung Tempe yang Terbuat dari Varietas Kedelai Impor dan Lokal Evaluation on Protein Nutritional Value of Tempe Flour Made from Imported and Local Soybean Varieties Made Astawan; Mursyid Mursyid; Deddy Muchtadi; Tutik Wresdiyati; Siti Harnina Bintari; Maryani Suwarno
JURNAL PANGAN Vol. 23 No. 1 (2014): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v23i1.48

Tempe merupakan produk olahan fermentasi kedelai asli Indonesia. Telah diketahui bahwa kandungan gizi tempe lebih baik dibandingkan kedelai yang tidak difermentasi. Masalah utama tempe adalah umur simpan yang relatif rendah. Salah satu alternatif pengolahan tempe adalah tepung tempe. Tujuan penelitian ini adalah untuk mengevaluasi kualitas protein tepung tempe yang terbuat dari kedelai impor dan lokal. Penelitian ini menggunakan tikus putih Sprague-Dawley sebagai hewan model. Tikus dibagi 4 kelompok berdasarkan sumber protein dalam ransum, yaitu tepung tempe kedelai impor Genetically Modified Organism (GMO)/hasil rekayasa genetika, tepung tempe kedelai impor non-GMO, tepung tempe kedelai lokal Grobogan, dan kasein sebagai kontrol. Parameter kualitas protein diukur berdasarkan metode pertumbuhan dan metode keseimbangan nitrogen. Hasil analisis menunjukkan tidak terdapat perbedaan yang nyata pada nilai food convertion efficiency (FCE), protein efficiency ratio (PER), dan net protein ratio (NPR) dari semua jenis tepung tempe. Nilai true protein digestibility (TPD) tepung tempe kedelai grobogan dan non-GMO tidak berbeda nyata, namun nyata lebih tinggi dari tepung tempe kedelai GMO, dan lebih rendah dari kasein. Tidak terdapat perbedaan yang nyata pada nilai biological value (BV) dan net protein utilization (NPU) semua sampel. Dari penelitian ini dapat disimpulkan bahwa secara umum nilai gizi protein tepung tempe kedelai lokal Grobogan tidak berbeda dengan tepung tempe kedelai impor non-GMO.Tempe is a fermented soybean product from Indonesia. It has been known that nutritional values of tempe are better than unfermented soybean. The main problem of tempe product is its short shelf life. An alternative way to solve this problem is tempe flour. The objective of this research was to evaluate the protein nutritional quality of tempe flours made from imported and local soybeans. This research used albino Sprague-Dawley rats as an animal model. The rats were divided into 4 groups based on protein source of the diet, namely: tempe flour of imported Genetically Modified Organism (GMO) soybean, tempe flour of imported non-GMO soybean, tempe flour of local Grobogan soybean, and casein as a control. Protein nutritional parameters were observed based on growth of rats and nitrogen balance methods. The results showed that there were no significant different of food conversion efficiency (FCE), protein efficiency ratio (PER), and net protein ratio (NPR) values from all of tempe flours. True protein digestibility (TPD) value of Grobogan and non-GMO tempe flours was not different, but higher than GMO soybean tempe flour, and lower than casein. There were no significant different of biological value (BV) and net protein utilization (NPU) values from all samples. This research concluded that generally the nutritional quality of protein of tempe flour from local Grobogan soybean tempe flour was not different from the protein quality of import non-GMOZT.

Karakteristik Fisikokimia dan Sifat Fungsional Tempe yang Dihasilkan dari Berbagai Varietas Kedelai (Phsyco-chemical Characteristics and Functional Properties of Tempe Made from Different Soybeans Varieties) Made Astawan; Tutik Wresdiyati; Sri Widowati; Siti Harnina Bintari; Nadya Ichsani
JURNAL PANGAN Vol. 22 No. 3 (2013): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v22i3.102

Tempe merupakan makanan tradisional Indonesia yang diproduksi melalui fermentasi kedelai dengan kapang Rhizopus sp. Tujuan dari penelitian ini adalah untuk membandingkan karakteristik fisik dan kimia kedelai impor (GMO, Non-GMO) dan kedelai lokal (Grobogan, Anjasmara, Argomulyo). Sebelum difermentasi, kelima jenis kedelai dibandingkan satu sama lain dalam hal ukuran, berat per 100 biji, volume, densitas kamba, impuritas, dan derajat pengembangan setelah dimasak dan direndam satu malam. Kadar air, abu, dan proteinnya juga dibandingkan. Untuk produksi tempe, kedelai disortasi, direbus, direndam, dikupas kulitnya, dan difermentasi. Tempe yang dihasilkan kemudian dianalisis kadar air, abu, protein, kapasitas antioksidan, rendemen, biaya paling efektif, dan karakteristik sensorinya. Hasil analisis menunjukkan kedelai Grobogan memiliki ukuran terbesar (19,53 g/100 biji kedelai) dan efektivitas biaya tertinggi (0,73), tetapi tidak berpengaruh nyata terhadap rendemen tempe yang dihasilkan (p > 0,05). Tempe yang dihasilkan dari kedelai Grobogan memiliki kadar air, protein, dan lemak yang sama dengan tempe dari kedelai impor. Tempe yang dihasilkan dari kedelai Argomulyo memiliki kadar protein tertinggi (52,70 persen). Kapasitas antioksidan tempe dari kedelai impor dan lokal berkisar antara 186-191 mg AEAC/kg tempe dan tidak berbeda nyata (p > 0,05) satu sama lain. Berdasarkan analisis sensori pada tempe mentah dan tempe goreng, secara keseluruhan tempe dari kedelai lokal memperoleh tingkat kesukaan yang sama dengan tempe dari kedelai impor.Tempe is Indonesian traditional food made by fermentation of soybean by the fungus Rhizopus sp. The objective of this research was to compare physical and chemical properties of import soybeans (GMO, Non-GMO) and local soybeans (Grobogan, Anjasmara, Argomulyo). Before being fermented, these import and local soybeans were compared on size, weight/100 grains, volume, bulk density, impurities, and puffing degree after being cooked and overnight soaked. The moisture, ash, and protein contents were also compared. For producing tempe, soybeans were sorted, cooked, soaked, dehulled, and fermented. The tempe moisture, ash, protein, antioxidant capacity, yield, cost effectiveness, and sensory characteristic were then evaluated. The result showed that Grobogan variety had the biggest size (19.53 g/100 soybean grains) and the highest cost effectiveness (0.73), but the yields of all tempe were not significantly different (p > 0.05). Tempe made from Grobogan soybean had moisture, protein, and fat content as high as tempe made from imported soybeans. Tempe made from Argomulyo soybean had the highest protein content (52.70 percent). The antioxidant capacity of tempe made from imported and local soybeans was about 186–191 mg AEAC/g, but was not significantly different (p > 0.05). Based on sensory evaluation of raw and fried tempe, overall tempe made from local soybeans had the same preference with tempe made from imported soybeans.

Co-Authors . Komari A.A. Ketut Agung Cahyawan W Abidah, Puri Adzrok Adi Winarto Adurrasyid, Zaid Afifah, Diana N. Akmal, Muhammad Ichlasul Alamsah Firdaus Alfarisi, Hamzah Amilia Dayatri Uray Anak Agung Istri Sri Wiadnyani Ananda Putri Cahyani Andi Early Febrinda Andi Mu’nisa Ani Karmila Ans Budi Hartanta ANZS BUDY HARTANTA Arief Boediono Armando M Saragih Aziz, Sandra Azizatul Ulfa Bambang Pontjo Priosoeryanto Bambang Purwantara Bella Dinar Fauqii Cahyani Berry Juliandi Chitisankul, Wanida T. Citra Noviana Dadang Supriatna Dadi Hidayat Maskar Dadi Hidayat Maskar, Dadi Hidayat Damayanti, Aprilia F. Damayanti, Aprilia Firdha Damiana Rita Ekastuti Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi DEDDY MUCHTADI Deddy Muchtadi Deddy Muchtadi Diini Fithriani Dimas Ahmad Rizaldi Dwi Febiyanti - Dwi Kesuma Sari Dwi Utami, Septi Eiichiro Fukusaki Eka Prasetiawan Eka Prasetiawan Eka Prasetiawan Elmeizy Arafah Erna Suzanna Evi Damayanti Evy Damayanthi Fithriani, Diini Fitrah Asma Ulhusna Fransiska R Zakaria Hadiningtias, Primanisa Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hamzah Alfarisi Hardinsyah Hidayati, Mustika I Komang Gede Wiryawan I Nyoman Suarsana Ichsani, Nadya Inas Suci Rahmawati Indah Fajarwati, Indah Indira Saputra Irma Isnafia Arief Isnafia Arief , Irma Ita Djuwita Jefriaman Sirait Joko Hermanianto Karnila, Rahman Karnila Ketut Adnyane Mudite koekoeh santoso Komang G Wiryawan Komari Komari Komari Komari Lasmiati, Ni Nengah LUSIA YUNI HASTANTI MADE ASTAWAN Made Astawan Made Astawan Made Darawati Manalu, Johanes Marojahan MARIA BINTANG Maryani Suwarno Maryani Suwarno Maryani Suwarno Muhamad Firdaus Muhamad Firdaus Muhammad Agus Muljanto Muhammad Ichlasul Akmal Muhammad Ichsan Mursyid . Mursyid Mursyid Mustika Hidayati Nadya Ichsani Nancy Dewi Yuliana NASTITI KUSUMORINI Nelis Imanningsih Ni Nengah Lasmiati Novita, Rias R. Palupi, Nurheni Sri Prabandari, Silvia Arin Prasetyawati, Renny Candra Prayudani, Ayu P G Prayudani, Ayu Putri Gitanjali Prima Yaumil Fajri Prima Yaumil Fajri Putri, Anisya Saeila Putri, Sastia P. Putri, Sastia Prama Putty Anggi Lestari Rafidha Irdiani Rahmawati, Siti Irma Ramdhani, Rizal Pauzan Ratnaningsih Eko S. Renny Candra Prasetyawati Ria Ceriana Rini Kesenja Rita Khairina Sadiah, Siti Saithong, Pramuan Sandra Arifin Aziz Saputra, Indira Saragih, Armando M SARASWATI SARASWATI Sarwono Waspadji Sarwono Waspadji Sarwono Waspadji Sastia Prama Putri Savitri Novelina Septi Dwi Utami Setyawati S Karyono Setyawati S. K. Setyawati S. Karyono Sidik . Silvia Arin Prabandari Siti Harnina Bintari Siti Sa'diah Siti Sa'diah Siti Sa'diah Siti Sadiah Siti Sa’diah SITI SA’DIAH Soewarno S Soekarto Soewarno Soekarto Sofiawati Sofiawati Sofiawati, Sofiawati Sri Rahmatul Laila Sri Widowati Sri Widowati Srihadi Agungpriyono Subangkit, Mawar Sukarno Sukarno Suliantari . Sussi Astuti Taopik Ridwan TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TEGUH SURANTA SINULINGGA Tessa Winandita Trioso Purnawan Tryas, Anisha Ayuning Ulhusna, Fitrah Asma VENNY FEBRIYANI Vera Di Nurwati Wasmen Manalu Yalmaida, Nabila Az Zahra Yana Nurdiana Yeni Setiorini Yeni Setiorini Yenni MS Nababan YOLI ZULFANEDI Yuspihana Fitrial

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