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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Farmasi Udayana Media Komunikasi FPIPS Majalah Kedokteran Bandung Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology JURNAL PENDIDIKAN KEPERAWATAN INDONESIA MPI (Media Pharmaceutica Indonesiana) INDONESIAN JOURNAL OF PHARMACY Acta Pharmaceutica Indonesia Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Jurnal Keperawatan Padjadjaran JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Indonesian Journal of Rheumatology JSKK (Jurnal Sains Keolahragaan dan Kesehatan) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal SPORTIF : Jurnal Penelitian Pembelajaran Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASI GALENIKA Jurnal Kesehatan Bakti Tunas Husada Borneo Journal of Pharmacy Kartika : Jurnal Ilmiah Farmasi Journal of Tropical Pharmacy and Chemistry Jurnal Farmasi Galenika Jurnal Analogi Hukum
I Ketut Adnyana
Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Journal : HAYATI Journal of Biosciences

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Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated From Tangkuban Perahu Mountain by Fermentation Desak Gede Sri Andayani; Ukan Sukandar; Elin Y. Sukandar; I. Ketut Adnyana
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.913 KB) | DOI: 10.4308/hjb.22.4.186

Abstract

Microorganisms were isolated from soil taken from Tangkuban Perahu mountain. Five strains were investigated in this study, designated TP1, TP2, TP3, TP4, and TP5, respectively. Morphological, biochemical and molecular identifications were conducted for all five strains. These isolates were shown to be closely related to Nocardia sp. YIM 65630 (90%), Streptomyces galbus (99%), Aspergillus unguis (86%), Paecilomyces marquandii (100%) and Nocardia niigatensis (95%), respectively. Production of antibacterial, antifungal and anticancer metabolites was done by fermentation. Screening for bioactivity of five isolates was done by testing the fermentation broth against resistant and pathogenic bacteria, fungi and T47D breast cancer cell line. TP2 strain showed the best bioactivity; the metabolite was purified by extraction with ethyl acetate. Antibacterial, antifungal and anticancer activities from the ethyl acetate extract of TP2 strain were tested by agar diffusion, microdilution and MTT. The extract was shown to be active against methicillin resistant Staphylococcus, methicillin sensitive Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, Escherichia coli, Microsforum gypseum with the minimum inhibitory concentration (μg/mL) and diameter of inhibition (mm): 150, 35; 150, 30; 300, 35; 300, 35; 300, 29; 4.7, 36, respectively. The IC50 value of the T47D cell line was 457 μg/mL.
Co-Authors - Liandhajani - Sukrasno . Patonah A Nawawi Afifa, Alia Tri Afifa, Alia Tri Agung Dwi Juniarsyah Alia Tri Afifa Ame Suciati Setiawan Andayani, Desak Gede Sri Andreanus A. Soemardji Andri Reza Rahmadi Apriantono, Tommy Armina, Tjokorde Istri Cempaka Kuning Christanti, Yohanna Dadan Resmana Dangin, I Gede Rama Andika Yuda Daryono Hadi Tjahjono Desak Gede Sri Andayani Desak Gede Sri Andayani DEWI SAFITRI Dewi Safitri Dewi Safitri Dewi, Sumartini Dwi Hadi Setya Palupi Eka Fitri Susiani Elin Y. Sukandar Elin Yulinah Elin Yulinah Sukandar Elin Yulinah Sukandar Elin Yulinah Sukandar Elin Yulinah Sukandar Elin Yulinah Sukandar Elin Yulinah Sukandar Elis Susilawati Elis Susilawati Finna Setiawan Fisheri, Neng Gunawan Pamudji Widodo Gusdinar, Tutus Hannan, Hannan Hasan, Muhamad Fahmi HENI RACHMAWATI Hikmat Permana I Gusti Agung Ayu Kartika I Made Agus Gelgel Wirasuta I NYOMAN SUTAMA Irda Fidrianny Irianti Bahana Maulida Reyaan Irvani Rakhmawati Iwo, Maria Immaculata Juniarsyah, Agung Dwi Juniarsyah, Agung Dwi Kartasasmita, R. E. Kogilavani Ramasamy Kuning, Cempaka Kusnaedi Kusnaedi Kusnandar Anggadiredja Leni Herliani Afrianti Leonny Yulita Hartiadi Lia Amalia Liandhajani, - Linda Amalia Lisna Anisa Fitriana, Lisna Anisa Maria Immaculata Iwo Mariani, Ria Marlina Indriastuti Mukti Priastomo Mulyawan, Rizki Nawawi, As’ari Nazhifa Ufamy Neng Fisheri Ni Kadek Warditiani Niken Indriyanti Niken Indriyanti Nisrina Dita Arlinda Nisrina Dita Arlinda, Nisrina Dita Nugrahani, Retta Poety Prawestyi Pratiwi Wikaningtyas Prawestyi, Poety Primal Sudjana Purwanti, Sandra Jati Rachmat Gunadi Wachjudi Rahmana Emran Kartasasmita Rahmatullah, Satrio Wibowo Rahmawati, Siti Farah Rakhmawati, Irvani Ramasamy, Kogilavani Ray, Hamidie Ronald Daniel Readi, Septa Reyaan, Irianti Bahana Maulida Rika Hartati Rini Syafriani Rini Syafriani Rosmadi, Arief Samsul Bahri Sandi, Dita Ayulia Dwi Sansan Candra Setiawan . Setiawan Setiawan Setiawan, Finna Sigit, Joseph Iskendiarso Slamet Ibrahim - Soemardji, Andreanus A. Sukandar, Elin Yulinah Sukandar, Elin Yulinah Sukandar, Ukan Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno, Sukrasno Suryani Suryani Susiani, Eka Fitri Susilawati, Elis Suwendar Suwendar Tommy Apriantono Tutus Gusdinar Kartawinata Ufamy, Nazhifa Ukan Sukandar Ukan Sukandar Widiati, Ida Ayu Putu Wikaningtyas, Pratiwi Wirasutisna, Komar Ruslan Wiyasa, Komang Ngurah Yani Mulyani Yani Mulyani Yedy Purwandi Sukmawan Yohanna Christanti Yuliet Yuliet Yuniarto, Ari Zazuli, Zulfan Zulfan Zazuli