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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ANNALES BOGORIENSES Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner Wahana Peternakan Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia

M Agus Setiadi

Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agathis, Kampus Dramaga IPB, Bogor, Jawa Barat, Indonesia 16880

Author-ID : 221524

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Efektivitas Ekstrak Biji Kapas (Gossypium hirsutum L.) terhadap Jumlah dan Viabilitas Embrio Mencit (Mus musculus L.) Nofri Zayani; Iman Supriatna; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The cottonseed (Gossypium hirsutum L.) contained gossypol as antifertility agent. The effect of cottonseed extract treatment could be decrease and impaired follicles development were accompanied by oocytesdamage. Damage of oocytes resulted reduction of number and viability of embryos. The objective of this study was to evaluate the effectiveness of cottonseed extract (Gossypium hirsutum L.) on the number and viability ofmice embryo (Mus musculus L.). Doses of the cottonseed extract were used consists of 0 (control), 1.5; 2.1; and 2.7 g/kg of body weight (BW) for 24 days via the oral route. This research used 24 animals healthy of femaleDDY mice 14-15 weeks old and 30-35 g BW. Embryos were collected at day 4 of pregnancy by flushing the utery cornua. The collected embryos were cultured in vitro for 48 hours in modified phosphate buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) culture medium according to the stage of its development to observe the viability of embryos. The result showed that the cottonseed extract with doses 1.5; 2.1; and 2.7 g/kg of body weight (BW) made the number of embryos which collected in D4 of pregnancy significantly lowerthan control (P<0.05). Data from embryos culture in vitro for 48 hours decreased embryos number (P<0.05) that developed in to the expanded and hatched blastocysts. At 2.7 g/kg BW, embryos only can develop to theblastocysts stage. Retardation (4-8 cells) and degeneration embryos did not develop in culture

Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan Sri Gustina; Hasbi Hasbi; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries.

Efektifitas Penambahan Insulin dalam Media Maturasi dan atau Media Kultur pada Tingkat Maturasi Oosit dan Perkembangan Awal Embrio Sapi secara In Vitro Ni Wayan Kurniani Karja; Syafri Nanda; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was aimed to determine the effect of insulin supplementation in maturation and/or culture medium on nuclear maturation rate and the early bovine embryo development in vitro. Oocytes were collected and matured in maturation media without (IVM I-) or with (IVM I+) 10 ug/µL insulin at incubator 5% CO2 and the temperature of 39 °C, for early embryonic development evaluation, oocytes were divided into 4 groups, without the supplementation of insulin to the maturation medium and culture (IVM I-/IVC I-), insulin supplementation only the maturation medium (IVM I+/IVC I-), insulin supplementation only in the culture medium (IVM I-/IVC I+), and the combination of insulin supplementation to the maturation medium and culture (IVM I+/IVC I+). The result showed that supplementation of insulin to the maturation medium increased number nuclear maturation was higher 87.7% (P<0.05) compared to treatment without supplementation of insulin (70.1%). Cleavage rate in treatment IVM was higher IVM I-/IVC I- (55.8%), IVM I+/IVC I- (64.1%), IVM I-/IVC I+ (59.9%) (P<0.05). Result of the other were showed that early bovine embryo on day-4 cultured (IVC) reached 16 cell on treatment IVM I-/IVC I+(31,9%) and IVM I+/IVC I+ (27,1%) were higher compared to treatment IVM I-/IVC I-(2.9%) and IVM I+/IVC I- (2.5%) (P<0.05). In conclusion, supplementation of insulin to maturation medium and culture medium can increase nuclear maturation rate and improved early embryo cleavage rate.

Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Ita Djuwita; Tuty Laswardi Yusuf; Mohamad Agus Setiadi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

The Effect of Vitamin E Supplement in the Diet on Gonad Maturation of Nilem (Osteochilus hasselti, CV) Nurbety Tarigan; Iman Supriatna; M. Agus Setiadi; Ridwan Affandi
Jurnal Perikanan Universitas Gadjah Mada Vol 19, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.26509

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving gonad maturation in nilem fish. In this study used a flockof fishbrood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg/kg. The results showed that fortifiedof vitamin E which were in feed significantly affected to the percentage of gonad development, gonadosomatic index (GSI), hepatosomatic index (IHS), fecundity, the diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity was 375 mg kg-1 with six weeks maintenance. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameters of the eggs were 0.31 mm, and the percentage of mature eggs was 64.44%. These results supported the potential of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in nilem fish thereby providing methods to improve fish reproduction

Potensi Alfa Enolase (ENO1) Membran Plasma Spermatozoa Sapi Bali Sebagai protein Antigenik Teguh Sumarsono; Bambang Purwantara; Iman Supriatna; Mohamad Agus Setiadi; Muhammad Agil
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.262-269

Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik.

Nuclear Maturation Competence and Pronuclear Formation in Ovine Oocytes Supplemented with L-ergothioneine in Maturation Medium Rimas Prathita Agustin; Mohamad Agus Setiadi; Iman Supriatna
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3120

This study aimed to determine the efficacy of supplementing maturation medium with L-ergothioneine on nuclear maturation competence and pronuclei formation of ovine oocytes in vitro. In the first study, oocytes were divided into four groups and matured for 24 hours in a maturation medium supplemented with 0 mM (control), 10 mM, 15 mM, and 20 mM LE. In the second study, oocytes were divided into two groups and matured for 24 hours in a maturation medium supplemented with 0 mM LE (control) and 10 mM LE (optimal dose from the first study), then co-incubated with sperm in a fertilization medium for 12 hours. The results of the first study showed that the maturation rate of oocytes enriched with LE 10 mM (88.74±1.79%) and 15 mM (87.52±2.30%) in maturation medium was significantly different (P0.05) compared to the control (78.55±1.78%) and LE 20 mM (77.37±1.21%). Furthermore, the results of the second study revealed that the fertilization rate of oocytes with 10 mM LE (75.55±3.47%) supplementation in maturation medium had a significantly higher percentage of two pronuclei formation than control (63.78±3.49%). In conclusion, supplementing 10 mM LE in a maturation medium improved oocyte nuclear maturation competence and fertilization rates.

Influence of Sperm Number and Antioxidant Melatonin in Extender on the Quality of Post-Thawing Sheep Spermatozoa Frilianty Putri; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Muyawati Kaiin
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3069

This study aimed to examine the effect of spermatozoa concentration and the effectiveness of melatonin supplementation in diluent on the quality of post-thawing semen. Ejaculated semen was collected using the artificial vaginal method (MVB). The study was carried out in two stages, firstly semen was frozen in andromed diluent with different concentrations in one straw (50, 100, 200 million per straw), and the second was frozen semen in diluent supplemented with melatonin with different doses (0, 0,5, 1, 0 and 1.5 mM melatonin). Parameters observed were the movement of spermatozoa using Computer Assisted Sperm Analysis, membrane integrity, and acrosome integrity. Data were analyzed using ANOVA and further tested using Duncan's test. The results showed no significant difference in the quality and movement pattern of sheep semen when frozen at concentrations of 50, 100, or 200 million per straw (P 0.05). Furthermore, adding melatonin to the diluent in this study affected spermatozoa's total motility and progressive motility at a concentration of 1.0 mM (P0.05) but did not significantly affect the percentage of spermatozoa motility pattern characteristics. From the results, it can be concluded that the concentration of per straw spermatozoa does not affect the quality of sheep semen, and melatonin supplementation in diluent can reduce the effects of the frozen-thawed process on motility, acrosome cap, and plasma membrane integrity in sheep semen. Melatonin supplementation with a concentration of 1 mM in the extender was the highest quality concentration in this study.

TINGKAT FERTILISASI OOSIT DOMBA DARI OVARIUM YANG DISIMPAN PADA SUHU DAN WAKTU YANG BERBEDA SECARA IN VITRO Arie Febretrisiana; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh suhu dan waktu penyimpanan ovarium terhadap tingkat fertilisasi oosit secara in vitro pada domba. Ovarium dibawa dari rumah potong hewan (RPH) dalam medium NaCl fisiologis pada suhu yang berbeda yaitu 27-28° C, 36-37° C, dan 4° C. Oosit kemudian dikoleksi dari setiap kelompok berdasarkan waktu penyimpanan yang berbeda yaitu 2-4, 5-7, dan 8-10 jam setelah domba dipotong. Oosit dikoleksi dan dimaturasi secara in vitro dalam inkubator 5% CO2, 38,5°C selama 28 jam. Oosit kemudian difertilisasi ke dalam drop spermatozoa selama 14 jam dalam inkubator CO2 5%, 38,5 C. Tingkat fertilisasi dievaluasi berdasarkan jumlah pronukleus yang terbentuk. Tingkat fertilisasi oosit yang dikoleksi dari ovarium yang disimpan pada suhu 27-28° C tidak berbeda dengan tingkat fertilisasi oosit yang disimpan pada suhu 36-37° C dan pada suhu 4° C, 2-4 jam setelah kematian hewan (masing-masing 53; 66,66; dan 63%) (P0,05). Tingkat fertilisasi oosit mulai mengalami penurunan pada tiga kelompok perlakuan setelah ovarium disimpan selama 8-10 jam, tingkat fertilisasi oosit yang disimpan pada suhu 27-28° C; 36-37° C; dan suhu 4° C masing-masing berturut-turut sebesar 9,8; 22,22; dan 12,24%. Dari hasil penelitian disimpulkan bahwa penyimpanan ovarium pada suhu 27-28° C dan 36-37° C selama 5-7 jam dapat mempertahankan kompetensi oosit dibandingkan dengan penyimpanan pada suhu 4° C.

KONSENTRASI, KEMURNIAN, DAN VIABILITAS SEL LEYDIG HASIL PURIFIKASI DENGAN GRADIEN NYCODENZ DAN KULTUR IN VITRO Ekayanti M. Kaiin; Ita Djuwita; Tuty L. Yusuf; M. Agus Setiadi
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan isolasi dan purifikasi sel Leydig dengan menggunakan gradien Nycodenz untuk meningkatkan jumlah perolehan sel Leydig yang hidup setelah purifikasi dan kultur. Isolasi dan purifikasi sel Leydig dilakukan dengan perlakuan gradien Nycodenz 3 kolom (5, 10, dan 15% sebagai Nycodenz I) dan 5 kolom (4, 8, 10, 12, dan 15% sebagai Nycodenz II) serta gradien Percoll 5 kolom (21, 26, 34, 40 dan 60%) sebagai kontrol. Parameter yang diamati adalah konsentrasi, kemurnian, dan viabilitas sel Leydig setelah isolasi dan purifikasi serta kultur in vitro. Hasil yang diperoleh menunjukkan bahwa isolasi dan purifikasi sel Leydig dengan gradien Nycodenz (I dan II) secara nyata (P0,01) menghasilkan konsentrasi sel yang lebih rendah dibandingkan dengan gradien Percoll. Namun penggunaan gradien Nycodenz II c enderung menghasilkan kemurnian sel yang lebih tinggi (91,40%) dibandingkan dengan Nycodenz I (85,53%). Hasil tersebut tidak berbeda nyata dibandingkan dengan gradien Percoll (92,20%). Viabilitas sel Leydig pada semua perlakuan hampir sama yaitu 98%. Namun demikia n, setelah dikultur selama 3 hari, konsentrasi sel Leydig pada perlakuan Percoll (2,44x106 sel/ml) dan Nycodenz I (3,21x106 sel/ml) secara statistik (P0,05) lebih rendah dan dibandingkan dengan Nycodenz II (3,88x10 6 sel/ml), sedangkan viabilitas sel Leydig setelah dikultur pada gradien Nycodenz I (90,00%) dan Nycodenz II (91,17%) secara sangat nyata (P0,01) menghasilkan viabilitas yang lebih tinggi dibandingkan dengan gradien Percoll (82,30%). Berdasarkan hasil tersebut dapat disimpulkan bahwa penggunaan gradien Nycodenz II efektif untuk mempurifikasi sel Leydig dan setelah dikultur menghasilkan konsentrasi dan viabilitas sel yang lebih tinggi dibandingkan dengan gradien Percoll.

Co-Authors . Hasbi Achmad Setiyono Adnin Adnan Agus Setiadi Agus Setiyono Alvien Nur Aini Amrozi Anak Agung Istri Sri Wiadnyani Ananda Ananda Anita Hafid Aras Prasetiyo Nugroho Arie Febretrisiana Arie Febretrisiana, Arie Arief Boediono Aries Boediono Ario Damar Asep Kurnia Asnath Maria Fuah, Asnath Maria Bambang Purwantara Bayu Rosadi Boenjamin Setiawan Chaniago, Rizky Amrullah Cristovao, Adelaide Jose Pereira Dadang Jaenudin Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti M. Kaiin Ekayanti M. Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Evy Damayanthi Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gustina, Sri Hadi Sumarno Hafizuddin Hafizuddin Harry Murti Hasbi . Hasbi Hasbi Hasim Heri Sujoko I Ketut Mudite Adnyana Idqan Fahmi Iman Supriatna IPB, DGB Ita Djuwita Ita Djuwita ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Kurnia, Asep Kusdiantoro Mohamad La Ode Muhammad Aswad Salam Lala M Kolopaking Lisa Dwi Fannessia Lisa Praharani Luki Abdullah M Agil M Noordin M. Elmanaviean M. Haviz M. Khoeron . Ma'mun Sarma Masir, Ummul Masturi Muhajir Mitha Kurnia Sari Mohamad Fahrudin Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron MULYOTO PANGESTU Musthamin Balumbi Nahrowi Neta Fitria Yasa Ni Wayan Kurniani Karja Nofri Zayani Novi Suprihatin Nurbety Tarigan Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oktariza, Wawan Praharani, Lisa Purwiyatno Hariyadi Rachmat Herman Rahmatullah Rahmatullah Rahmatullah Rahmatullah Reski Adelia Ridwan Affandi Rimas Prathita Agustin Rimayanti - Rizal Gusdinar Ronny Rachman Noor Sari, Mitha Kurnia Satya Gunawan Somanjaya, Rachmat Soni Sopiyana Sri Gustina Sri Purwaningsih, Sri SRI RAHAYU Sri Rahayu Srihadi Agungpriyono Sudradjat Sumiati Suria Darma Tarigan Suwandi, Syifa Damaianti Syafri Nanda Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Teguh Sumarsono Tuty L. Yusuf Tuty L. Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ulfah Juniarti Siregar Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina YULNAWATI YULNAWATI Yusmala, Mitha Zultinur Muttaqin

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