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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ANNALES BOGORIENSES Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner Wahana Peternakan Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika

M Agus Setiadi

Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agathis, Kampus Dramaga IPB, Bogor, Jawa Barat, Indonesia 16880

Author-ID : 221524

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Efektivitas Insulin-Like Growth Factor-I (IGF-I) dalam Media Maturasi In Vitro Pada Pematangan Inti dan Fertilisasi Oosit Sapi Bali . Hasbi; Sri Gustina; Ni Wayan Kurniani Karja; Iman Supriatna; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Penelitian ini bertujuan mengetahui efektivitas insulin-like growth factor–I (IGF-I) pada tingkat pematangan inti dan fertilisasi oosit sapi bali. Penelitian ini dibagi dalam dua tahap. Tahap I, oosit dimatangkan secara in vitro dalam media 199 dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I. Tahap II, oosit dimatangkan dalam media seperti pada penelitian tahap I, kemudian difertilisasi secara in vitro untuk mengamati pembentukan pronukleus. Hasil penelitian tahap I menunjukkan bahwa tidak terdapat perbedaan jumLah oosit yang mencapai tahap metaphase II (MII) dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I dalam media maturasi. Berturut-turut adalah 80,6±7,6%; 81,5±8,6%; 87,5±6,9%; dan 84,1±12,4%. Penelitian tahap II menunjukkan bahwa tingkat fertilisasi pada penambahan 100 ng/mL IGF-I dalam media maturasi nyata lebih tinggi (P<0,05) dibandingkan dengan kontrol dan penambahan 50 ng/mL IGF-I, yaitu berturut-turut 78,3±6,6%, 67,1±8,9%, dan 64,6±6,0% untuk dosis 100, 0, dan 50 ng/mL. Akan tetapi, peningkatan dosis pemberian IGF-I menjadi 150 ng/mL tidak meningkatkan tingkat fertilisasi yaitu 73,5±9,3%. Dari hasil penelitian disimpulkan bahwapenambahan IGF-I dalam media maturasi tidak mampu meningkatkan jumLah oosit yang mencapai tahap MII, namun penambahan 100 ng/mL IGF-I dapat meningkatkan jumLah oosit yang terfertilisasi.

Kompetensi Maturasi Oosit in vitro dan Kajian Histologi Folikel dari Ovarium Domba Pascapenyimpanan pada Suhu 4°C Masturi Muhajir; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; I Ketut Mudite Adnyane
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Penelitian ini bertujuan untuk mengkaji kompetensi maturasi oosit secara in vitro dan gambaran histologi ovarium pascapenyimpanan ovarium pada suhu 4°C selama empat hari. Ovarium dari rumah potong hewan dibagi menjadi 4 kelompok dan disimpan pada suhu 4°C selama 0 jam (kelompok H-0/kontrol), 24 jam (Kelompok H-1), 48 jam (Kelompok H-2), 72 jam (Kelompok H-3) dan 96 jam (Kelompok H4). Pada setiap akhir periode penyimpanan, oosit dikoleksi dan diseleksi berdasarkan keadaaan kekompakan sel-sel kumulus, kehomogenan dari sitoplasma (Grade A sampai C). Oosit dengan grade A dan B dimaturasi secara in vitro selama 24 jam. Gambaran folikel dalam ovarium pascapenyimpanan dikaji dengan pewarnaan Hematoksilin-Eosin. Terjadi penurunan yang signifikan (P<0.05) pada jumlah oosit dengan grade A setelah hari kedua penyimpanan. Kemampuan oosit untuk mencapai MII menurun setelah penyimpanan hari kedua (P<0.05). Seiring dengan penurunan jumlah oosit yang mencapai MII, terjadi peningkatan jumlah oosit yang mengalami degenerasi pada hari ketiga dan keempat pascapenyimpanan ovarium (P<0.05). Dari gambaran histologi, ditemukan adanya folikel yang mengalami piknotik setelah penyimpanan 24 jam. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas oosit persentase oosit mencapai MII menurun setelah penyimpanan 24 jam. Terjadi perubahan struktur sel dan degenerasi dari oosit pada gambaran histologi folikel dalam ovarium.

The use enzyme immuno assay methode for measurement of milk progresterone without extraction for early pregnancy diagnosis in cow Adnin Adnan; M. Agus Setiadi; M. Khoeron .
Hemera Zoa Vol. 77 No. 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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An Enzyme Immuno Assay for Progesterone, using horse radish peroxidase as the label, was adapted for direct measurement of progesterone in milk. This was carriedout to detect early pregnancy in cows. The experiment used sixty milking cows divided into three groups, one as a controland two others as treatment groups. In group I (control) milk samples were collected on the day of insemination to 24 days afterward by quartan collection. In group II, milk samples were collected on days 18, 21 and 24 after insemination. In group III, milk samples were collected on days 21 and 24 after insemination. Milk samples were collected from lactating cows at noon from four quarters and preserved with Potassium dichromate (Merck 4858), stored at - 20 degree C until analysed.Pregnancy diagnosis by EIA was confirmed by rectal palpation at 60 to 90 days after insemination. The early pregnancy diagnosis in Group II compared with Group III showed that prolonged the time of sampling indeclinable improved the acuracy.

Perbandingan Hasil Penyerentakan Berahi pada Sapi Perah Setelah Pemberian Progesteron-CIDR dan Prostaglandin M Agus Setiadi; M Noordin; M Agil
Hemera Zoa Vol. 75 No. 2 (1992): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Penyerentakan berahi telah dilakukan pada 3 kelompok (30 ekor) sapi perah laktasi, 2-3 bulan setelah melahirkan.Kelompok 1 menggunakan Control Internal Drug Releasing device (CIDR) yang mengandung Progesteron, kelompok 2 mnggunakan Prostaglandin dosis tunggal dan kelompok 3 sebagai kontrol menggunakan NaCl fisiologis.Gejala berahi diamati 24 jam setelah perlakuan masing-masing pada kelompok 2 dan 3.Secara statistik, berahi lebih serentak terjadi pada kelompok 1 dan 2 dibandingkan dengan kelompok 3 dan tidak ada perbedaan nyata antara kelompok 1 dan 2.

Status of ram spermatozoa DNA after freeze-drying process Takdir Saili; wahono Esthi Prasetyaningtyas; Mohamad Agus Setiadi; Srihadi AgungPriyono; Arief Boediono
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK) Soni Sopiyana; Iman Supriatna; M. Agus Setiadi; Mohamad Fahrudin
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In poultry embryos, primordial germ cells (PGCs) are progenitor cells for gametes, which have unique migration pathway. Primordial germ cells arise from epiblast in germinal crescent and circulate through the bloodstream for a short period of time, then leave blood vessel to migrate toward gonads. The aim of this study was to determine the potential production capacity of circulated-PGCs of KUB chicken at different developmental stages of embryo using a rapid and simple method. Seventy five KUB chicken fertile eggs were divided into five groups and incubated at 38.5 0C with a humidity of 60%. Hatching was set to the embryonic development stage of 14-18. The blood was collected through dorsal aorta using micropipette under microscope. The collected blood was placed in a 1.5 ml eppendorf tube which was previously filled with 100 µl phosphate buffered saline without Ca2+ and Mg2+ (PBS-) mixed with fetal bovine serum (FBS) with a ratio of 90%:10%. The PGCs were purified using lysis buffer ammonium chloride potassium method. The results showed that average production of circulated-PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P <0.05). The average production of circulated-PGCs at stage 14, 15, 16, 17, and 18 were 37.9; 53.5; 49.8; 38.3; and 33.5 respectively. The number of circulated-PGCs was not different among stages 14, 17 nor 18. The highest number of circulated-PGCs of KUB chicken was obtained at stage 15, so that the isolation and collection of PGCs through the blood circulation was recommended in stage 15.Key Words: KUB Chicken, PGCs, Embryonic Development Stage, Ammonium Chloride Potassium

Follicular dynamic and repeatability of follicular wave development in Peranakan Ongole (PO) cattle Muhammad Imron; Iman Supriatna; . Amrozi; Mohamad Agus Setiadi
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Superovulation treatment on PO cattle (Bos indicus) was less responsive compared to Bos taurus breed. It might due to the difference of their follicular dynamic. This study was conducted to investigate the follicular dynamics and its repeatability in PO cattle. Follicular dynamics observations conducted on 9 cows trough ultrasound scanning every day. Observations of wave patterns repeatability were performed in 6 cows which its wave pattern already known on the next consecutive IOI. Research result indicated that PO cattle had 3 (66%) and 4-waves (34%) pattern. The first wave of 3 and 4-waves pattern emerged on day -0.4+0.9 and 1.4+1.1 respectively. The second wave of 3 and 4-wave pattern emerged on day 9.8+1.5 and 7.4+1.9 respectively. The pattern of 3 waves has a longer follicle dominant duration (11.6+1.5 day) in the first wave of estrous cycle, compared with 4 waves pattern (10+2.92 and 7+1.00 day respectively). The growth rate of dominant follicle was not different significantly between the 3 and 4-waves pattern (0.87+0.23 and 0.94+0.25 mm/day respectively). Similarly, ovulatory follicle diameter between 3 and 4-waves pattern was also not different significantly (12.24+12.34 and 12.30+12.23 mm respectively). Observation of wave patterns repeatability in 6 PO cows indicated that PO cattle had high repeatability in follicular wave pattern (0.88) and the number of growing follicle was 0.91. This study resulted data for dynamic of follicular development, wave pattern, its repeatability which be expected to design the protocol of superovulation treatment or other reproduction technologies based on follicular dynamic to improve its result in PO cattle.

Adiponectin: Potential Protein Hormone as a Candidate Biomarker for Male Fertility H Hafizuddin; Ni Wayan Kurniani Karja; Lisa Praharani; Mohamad Agus Setiadi
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 29, No 4 (2019): December 2019
Publisher : Indonesian Center for Animal Research and Development

Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarker, by intense research on fertility-associated proteins contained in seminal plasma. This paper describes the role of adiponectin as a biomarker candidate of male fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.

Intracytoplasmic Sperm Injection (Icsi) Sebagai Teknik Reproduksi Bantuan Unggulan Takdir Syahruddin Said; Syahruddin Said; Mohamad Agus Setiadi; Srihadi Agungpriyono; Mozes R. Toelihere; Aries Boediono
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Beberapa teknik fertilisasi mikro telah diterapkan untuk mengatasi masalah infertilitas pria, namun hanya intracytoplasmic sperm injection (ICSI) yang mampu meningkatkan secara nyata keberhasilan fertilisasi, implantasi dan kelahiran. Pada perkembangannya teknik ICSI juga telah diterapkan pada beberapa jenis hewan sebagai suatu model untuk mempelajari kemampuan fertilisasi berbagai jenis spermatozoa yang secara alami atau melalui fertilisasi in vitro tidal( bisa membuahi sel telur. Walaupun keberhasilan teknik ICSI telah dapat mengatasi masalah infertilitas pada pria, teknik ICSI masih mempunyai potensi negatif yang mungkin muncul pada anak hasil ICSI. Namun demikian, hal ini perlu penelitian lebih lanjut untuk membuktikannya.

Kemampuan Fertilisasi Spermatozoa Sexing dan Perkembangan Awal Embrio Secara In Vitro pada Sapi Alvien Nur Aini; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of the present study was to investigate the fertilization ability of bovine oocytes and early bovine embryonic development in vitro, fertilized by frozen X and Y sperm separated by bovine serum albumin (BSA) gradient column. Oocytes were collected from slaughter house ovarian by flushing and slicing technique. Oocytes were than maturated in tissue culture medium (TCM) 199 supplemented with 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG) and 10% fetal bovine serum (FBS) for 24 h in 5% CO2 incubator 39oC. Oocytes then fertilized with three kind of different frozen spermatozoa (X,Y and unsexing spermatozoa as control) for 14 h with final concentration 2x106 spermatozoa/mL. Embryos were cultured insynthetic oviductal fluid (SOF) supplemented with essential and non essential amino acid and 0.3% bovine serum albumin (BSA) for 96 h. Results of the experiments revealed that there was no significant difference (P>0.05) in thefertilization ability (49.17%; 51.40%; 53.42%) for X, Y and control group, respectively. No significant difference (P>0.05) in the number of embryos development (47.77%; 48.25%; 54.43%) for X, Y and control group, respectively. Furthermore, only small number of embryos could pass development blockade (23.80%; 26.08%; 23.61%) for X, Y and control spermatozoa with statistically no significant difference (P>0.05). It is concluded that sexed spermatozoa separated by BSA gradient column had comparable fertilization ability with unsexing spermatozoa and had ability to supported early embryonic development.

Co-Authors . Hasbi Achmad Setiyono Adnin Adnan Agus Setiadi Agus Setiyono Alvien Nur Aini Amrozi Anak Agung Istri Sri Wiadnyani Ananda Ananda Anita Hafid Aras Prasetiyo Nugroho Arie Febretrisiana Arief Boediono Aries Boediono Ario Damar Asep Kurnia Asnath Maria Fuah, Asnath Maria Bambang Purwantara Bayu Rosadi Boenjamin Setiawan Dadang Jaenudin Dondin Sajuthi Ekayanti M. Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Evy Damayanthi Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gustina, Sri Hadi Sumarno Hafizuddin Hafizuddin Harry Murti Hasbi . Hasbi Hasbi Hasim Heri Sujoko I Ketut Mudite Adnyana Idqan Fahmi Iman Supriatna IPB, DGB Ita Djuwita Ita Djuwita Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Kusdiantoro Mohamad La Ode Muhammad Aswad Salam Lala M Kolopaking Lisa Dwi Fannessia Lisa Praharani Luki Abdullah M Agil M Noordin M. Haviz M. Khoeron . Ma'mun Sarma Masir, Ummul Masturi Muhajir Mitha Kurnia Sari Mohamad Fahrudin Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron MULYOTO PANGESTU Musthamin Balumbi Nahrowi Neta Fitria Yasa Ni Wayan Kurniani Karja Nofri Zayani Novi Suprihatin Nurbety Tarigan Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oktariza, Wawan Praharani, Lisa Purwiyatno Hariyadi Rachmat Herman Rahmatullah Rahmatullah Reski Adelia Ridwan Affandi Rimas Prathita Agustin Rimayanti - Rizky Amrullah Chaniago Ronny Rachman Noor Satya Gunawan Somanjaya, Rachmat Soni Sopiyana Sri Gustina Sri Purwaningsih, Sri SRI RAHAYU Sri Rahayu Srihadi Agungpriyono Sudradjat Sumiati Suria Darma Tarigan Syafri Nanda Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Teguh Sumarsono Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Ulfah Juniarti Siregar Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina YULNAWATI YULNAWATI Zultinur Muttaqin

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