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All Journal International Journal of Evaluation and Research in Education (IJERE) Prosiding Seminar Biologi EDUSAINS QUALITY WARTA Jurnal Varidika Bioeksperimen: Jurnal Penelitian Biologi Forum Geografi Manajemen Pendidikan QALAMUNA: Jurnal Pendidikan, Sosial, dan Agama Jurnal Penelitian Pendidikan IPA (JPPIPA) Aqlam: Journal of Islam and Plurality Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan JMKSP (Jurnal Manajemen, Kepemimpinan, dan Supervisi Pendidikan) NUSANTARA : Jurnal Ilmu Pengetahuan Sosial Jurnal Basicedu Indonesian Journal on Learning and Advanced Education (IJOLAE) Jurnal EDUCATIO: Jurnal Pendidikan Indonesia Jurnal Gentala Pendidikan Dasar utile: Jurnal Kependidikan Buletin KKN Pendidikan Jurnal Riset Biologi dan Aplikasinya Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Didaktika: Jurnal Kependidikan Jurnal Kewarganegaraan Jurnal Riset dan Inovasi Pembelajaran Jurnal Ilmiah Kampus Mengajar Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Jurnal Basicedu Urecol Journal. Part A: Education and Training Prosiding University Research Colloquium Warta LPM Jurnal Elementaria Edukasia J-CEKI
Sofyan Anif
Universitas Muhammadiyah Surakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : Jurnal Riset Biologi dan Aplikasinya

 1 
Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Co-Authors A.A. Ketut Agung Cahyawan W Abdurrahman, Shemsu Gupta Achmad Fathoni Achmad Fathoni, Achmad Afif Iqbal Alviandi Ahmad Muhibbin Ahmad Muhibbin Alanindra Saputra Alanindra Saputra Alfian Chrisna Aji Almas Al Jinan Almuntaqo Zain Almuntaqo Zain, Almuntaqo Alqudsi, Zainab Alvia Quronita Ayunisai Sholihah Anam Sutopo Anatri Desstya Andre Eksaputra Nugraha Ardheila Setya Arif Hidayat Arifah, Ika Nur Ariyanti, Olivia Puja Aryati Prasetyarini Aviandri Cahya Nugroho Bambang Sumardjoko Budi Murtiyasa Che Nidzam Che Ahmad Desi Yulihapsari Destama Einstein Shodiq DIAN LESTARI Dian patriana, Wendy Djalal Fuadi Dwi Rizaldi Hatmoko Dwi Setyo Astuti Efi Miftah Faridli, Efi Miftah Eko Wijiyono Endang Fauziati Endang Setyaningsih Farita Lina Rodiyah Fathul Zannah Fatkhurohmah Fitri Rahma Octaviani Fitriyatno Fitriyatno Gamal Abdul Nasir Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hariyatmi Harsono, H Harun Joko Prayitno Heri Widiyantoro Heri Widiyantoro, Heri Hernawan Sulistyanto Heshinta, Linda Hidayat, Ma’ruf Husni Mubarok Idawati Idawati Ilham Mujahidin Indah Kusuma Wardani Iyan Sofyan Jamil, Nazia Jumadi Jumadi Kusuma Budi, Anggun Laila Nur Izzati Lestari, Ririn Purwani Mardi Ma’ruf Hidayat Mandiri, Listy Hasti Markamah, Markamah Maryadi Maryadi Mauly Halwat Hikmat Mazlini Adnan Mazwar, Mazwar Ismiyanto Meggy Novitasari Milan Sabekti Minsih Mohammad Rudiyanto Mohammad Rudiyanto Muhammad Izzul Haq Muhammad Luthfi Hidayat Muhammad Syahriandi Adhantoro Muhibbin, Muhibbin Muhtadi Muhtadi Muhtadi Muhtadi Mujahidin Mujahidin Muliasari, Kharina Cahya Murdiani, Janti Mustofa, Rosit Novia Citra Paringsih Nurgiyatna Patriana, Wendy Dian Prabawati, Ratna Probowati, Annisa Purnawanto, Ahmad Teguh Putri Agustina Rayana, Anis Retno Susiyani Ritantiyo, Toni Riza Rifai rohmah, rohmah Rosit Mustofa S. Sukartono Sabar Narimo Sania Rahayu Sastra, Anita Anugerah Seno, Hernandito Aryo Septinawati, Septinawati Setyaningrum, Fadtma Alfita Sigit Haryanto Siti Hadiyati Nur Hafida Siti Nuraini Siti Nurfaida Sopianti, Herlin SRI RAHAYU Sukartono Sukartono Sumardi . Sumardi Sumardi Sunanih Sunariati, Rintik Suparti - - Suryawati, Andari Tri Sutama Suyatmini Suyatmini Tahang, Heriyanti Teguh Waluyo Triastuti Rahayu Tuti Rahmawati Ulya, Walidatul Umardhani, Niluh Sekar Zulfa Wahyuningsih, Wiwin Wan Nasriha Wan Mohamed Salleh Weni Hastuti Weni Hastuti Wijayanti, Ratih Kumala Wijiyono, Eko Wiwin Wahyuningsih Yasir Sidiq Yustin, Yustina Neny Hastuti Zainab Alqudsi Zulperi, Dzarifah