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Laboratorium Patologi Klinik, Fakultas Kedokteran Hewan, Univesitas Udayana, Jl. Raya Sesetan, Gg. Markisa No. 6. Denpasar Bali
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Pemberian Tambahan Tepung Belatung atau Maggot Lalat Hermetia illucens Dalam Pakan Broiler Meningkatkan Aspartate Aminotransferase dan Menurunkan Alanine Aminotransferase Qutrotu'ain, Salsabila; Ardana, Ida Bagus Komang; Siswanto, Siswanto
Indonesia Medicus Veterinus Vol 11 (1) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.1.76

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung maggot BSF terhadap AST dan ALT broiler. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 24 ekor ayam pedaging (broiler) jantan, dengan empat perlakuan dan enam ulangan. Perlakuan yang diterapkan adalah (P0) 100% pakan komersial, (P1) pakan komersial ditambahkan 1% tepung belatung/maggot BSF, (P2) pakan komersial ditambahkan 2% tepung belatung/maggot BSF, dan (P3) pakan komersial ditambahkan 3% tepung belatung/maggot BSF. Pengambilan sampel darah melalui vena brachialis. Data yang diperoleh dari penelitian ini dianalisis dengan uji sidik Ragam. Pada penelitian ini nilai AST P0, P1, P2 dan P3 masing-masing adalah 249,17 U/L; 341,17 U/L; 307,83 U/L; 340,17 U/L. Sedangkan nilai ALT P0, P1, P2 dan P3 masing-masing adalah 70,83 U/L; 46,17 U/L; 43,67 U/L; 44,33 U/L. Hasil penelitian menunjukkan bahwa pemberian tepung maggot BSF berpengaruh secara signifikan terhadap AST dan ALT broiler. Kesimpulan yang diperoleh yaitu pemberian tepung maggot BSF dapat meningkatkan kadar AST dan menurunkan kadar ALT namun masih dalam kadar yang normal.
Total Leukosit dan Diferensial Leukosit Ayam Pedaging yang diberi Tepung Belatung sebagai Pakan Tambahan Ransum Lubis, Barata Sultan; Ardana, Ida Bagus Komang; Siswanto, Siswanto
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.877

Abstract

Tepung belatung Hermetia illucens memiliki kandungan protein 37,31% dan lemak 39,05%, sehingga dapat digunakan sebagai pakan tambahan dalam ransum. Sebagai bahan yang kaya dengan protein dan lemak, belatung black soldier fly (BSF) baik pula hubungannya dengan pembentukan sel darah putih (leukosit). Penelitian ini bertujuan untuk mengetahui total leukosit dan diferensial leukosit ayam pedaging yang diberi tepung belatung sebagai pakan tambahan dalam ransum. Sebanyak 24 ekor ayam pedaging jantan digunakan dalam penelitian ini. Ayam pedaging dikelompokkan menjadi empat sesuai dengan perlakuan: perlakuan P0 (kontrol), perlakuan P1 (1% dari pakan komersial), perlakuan P2 (2% dari pakan komersial), dan perlakuan P3 (3% dari pakan komersial). Perlakuan diberikan mulai hari ke-14 sampai hari ke-35. Sampel darah diambil dari vena pectoralis sebanyak 3 mL pada hari ke-36. Jumlah leukosit dan diferensial leukosit dihitung menggunakan auto hematology analyzer. Data yang telah diperoleh dianalisis menggunakan sidik ragam satu arah. Hasil penelitian menunjukkan total leukosit 81,92-97,98×103/µL, persentase heterofil 28,50-37,50%, persentase eosinofil 0%, persentase basofil 0%, persentase limfosit 51,67-64,17%, dan persentase monosit 7,33-10,83%. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan tepung belatung ke dalam pakan komersial meningkatkan total leukosit, persentase limfosit, dan menekan persentase heterofil ayam pedaging.
Total Eritrosit, Kadar Hemoglobin, dan Nilai Packed Cell Volume Broiler Setelah Penambahan Acidifier Asam Organik dan Anorganik dalam Pakan Sidabutar, Febyana; Ardana, Ida Bagus Komang; Suada, I Ketut
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.187

Abstract

Penggunaan asam organik sebagai pengganti antibiotik sangat efektif. Tujuan penelitian adalah untuk mengetahui efek pemberian kombinasi asam organik dan anorganik terhadap total eritrosit, kadar hemoglobin dan nilai hematokrit broiler. Perlakuan yang diberikan adalah P0 adalah broiler yang mendapat pakan tanpa kombinasi asam organik dan anorganik, P1 adalah broiler yang diberi pakan ditambah asam organik dan anorganik 1 g/kg pakan, P2 adalah broiler yang diberi pakan ditambah asam organik dan anorganik 2 g/kg pakan dan P3 adalah broiler yang diberi pakan ditambah asam organik dan anorganik 3 g/kg pakan. Hasil pengamatan dianalisis menggunakan hematology analyser, pemeriksaan sampel dilakukan di Balai Besar Veteriner Denpasar. Sampel yang digunakan adalah sampel darah dari 24 ekor broiler yang diberi perlakuan berbeda. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Data yang diperoleh dari penelitian ini dianalisis dengan menggunakan uji sidik ragam. Pada penelitian ini didapatkan hasil bahwa penambahan kombinasi asam organik dan anorganik asam organik dan anorganik tidak berpengaruh nyata terhadap total eritrosit, kadar hemoglobin, dan nilai packed cell volume atau hematokrit (P>0,05). Dapat disimpulkan bahwa pemberian asam organik dan anorganik tidak memengaruhi (mempertahankan) total eritrosit, kadar hemoglobin, dan nilai packed cell volume broiler. Dapat disimpulkan bahwa pemberian tidak memengaruhi (mempertahankan) total eritrosit, kadar hemoglobin, dan nilai packed cell volume broiler.
PELAYANAN KESEHATAN DAN PEMBERANTASAN PENYAKIT TERNAK SAPI BALI DALAM MENDUKUNG PROGRAM SWASEMBADA DAGING SAPI DI DESA BURUAN BLAHBATUH GIANYAR A.A. G. O. Dharmayudha; Made Suma Anthara; I M. Sukada; I B. Komang Ardana
Buletin Udayana Mengabdi Vol 18 No 1 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.042 KB) | DOI: 10.24843/BUM.2019.v18.i01.p19

Abstract

Pengabdian kepada masyarakat yang dilakukan di desa Buruan, Kecamatan Blahbatuh, Kabupaten Gianyar ini pada intinya bertujuan untuk menekan angka kerugian ekonomi yang disebabkan oleh masalah kesehatan sapi bali. Metode yang digunakan dalam pengabdian masyarakat ini yaitu dalam bentuk pelayanan kesehatan dari rumah ke rumah berupa pemberian vitamin, obat cacing terhadap ternak yang sehat dan melakukan pengobatan terhadap ternak yang sakit, serta diskusi dengan peternak dalam hal ini manjemen pemeliharaan ternak (khususnya sapi bali), serta masalah lainnya seperti penyakit yang umum pada sapi bali dan masalah reproduksi sapi bali. Hasil yang didapat yaitu 22 ekor sapi (20 betina, 2 jantan). Diharapkan melalui pengabdian ini, pada ternak sapi yang sakit akan terjadi peningkatan kesehatan sapi dan menekan angka kejadian penyakit, serta meningkatkan produksi dan kualitas daging sapi secara optimal sehingga program pemerintah yaitu swasembada daging sapi dapat terus terlaksana. Kata kunci : obat cacing, sapi bali, vitamin.
SOSIALISASI PENYAKIT RABIES PADA SISWA SLTA DI DESA BEBANDEM KARANGASEM Dharmawan. N. S.; I M. Damriyasa; I. B. K. Ardana; A. A. S. Kendran; K. K. Agustina
Buletin Udayana Mengabdi Vol 10 No 2 (2011): Volume 10 No.2 – September 2011
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

This Community Service was conducted on October 23rd. 2010 in Bebandem village, Karangasem regency in the form of socialization of rabies on senior high school students. The aim of this program was to give knowledge, clinical symptoms, transmission, and prevention of the rabies diseases. There were 50 participants who came from representatives of each class. Twenty-eight (56%) participants reported that at their house, they have dogs and cats, mostly (50%) have not been vaccinated. The activity runs smoothly and as planned. Socialization of rabies diseases has improved the understanding of all participants. At the end of the event, most of them (90%) reported that they were satisfied and there are 48 (96%) participants are interested in becoming a volunteer to join to inform rabies to other school children.
Profil Kimia Klinik Darah Sapi Bali (BLOOD CLINICAL CHEMISTRY PROFILES OF THE BALI CATTLE) Anak Agung Sagung Kendran; I Made Damriyasa; Nyoman Sadra Dharmawan; Ida Bagus Komang Ardana; Luh Dewi Anggreni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

For the evaluation of clinical tests in veterinary laboratories a reference basis of normal values ofclinically healthy farm animals is essential. It is well known that variables such as breed, age and sex havean influence on many blood parameters. The reference value of blood chemistry of bali cattle is scanty. Theaim of the current study was to evaluate blood chemistry of bali cattle includes alanine aminotransferase(ALT), aspartate aminotransferase (AST), urea, creatinine and glucose, measured by an auto analyzer.These data were used to establish reference intervals in bali cattle of different ages and sex. Blood wascollected from 195 (21 young males, 54 adult males, 60 young females, and 60 adult females) clinicallyhealthy bali cattle by means of jugular venipuncture. Values from different age and sex were comparedstatistically. The results of the study was obtained that ALT and blood glucose significantly higher inmales Bali cattle (P <0.05) then in females. ALT, AST and urea were significantly higher in adults Balicattle (P<0.05) then in young Bali cattle. However, glucose of young Bali cattle was higher when comparedwith the adult. There was no significant difference in the creatinine levels between adults and the youngones. The results of the current study provide reference intervals for clinical chemistry of Bali cattle
Vaksin Gumboro Menyebabkan Imunosupresif pada Respons Primer Vaksin Penyakit Tetelo Ayam Pedaging Gusti Ayu Yuniati Kencana; Anak Agung Ayu Mirah Adi; Ida Bagus Komang Ardana; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.777 KB)

Abstract

The variety of Gumboro live vaccine strains (hot, intermediate, and mild) are available inIndonesia. The immunosuppresive effect of these vaccines under field conditions is not known.This research was conducted to determine this devastating effect of such vaccine strains on theimmune response of chickens vaccinated againts Newcastle disease (ND). Sixty chickens werekept separately in five groups (i.e. V1, V2, V3, V4, and K). At the age of seven days, group V1, V2,and V3 were given hot, intermediate, and mild strains of Gumboro live vaccine respectively whilethe other two groups recieved no Gumboro vaccine (V4 and K). At the age of 14 days, all groups,except group K which were kept as a negative control, were vaccinated against ND. The level ofantibody produced in response to ND vaccination was measured in sera collected at day 0, 7, 14,and 21 post ND vaccination using a standard micro-haemaglutination inhibition test. Data of theantibody titers were analyzed using analysis of variance followed by Duncan’s multiple range test.The results showed that all Gumboro vaccine strains still retain its immunosuppressive nature onhumoral immune response in chickens that later vaccinated against ND. The geometric meantiter (GMT) of anti-NDV antibody of group V4 (unvaccinated againts Gumboro) was significantlyhigher than that of group V1, V2, and V3, i.e. groups of chickens that had been given varietystrains of Gumboro vaccines, at the first and second week after ND vaccination (p<0.05). Thedifference of this immunosuppressivenes among variety of Gumboro vaccine strains need furtherclarification.
Pemakaian Herbal Serbuk Biji Pepaya Matang dalam Pengendalian Infeksi Ascaris suum pada Babi Ida Bagus Komang Ardana; I Made Bakta; I Made Damriyasa
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study on vermisidal effect of ripe papaya seed powder against egg and worm of A.suum in landracepig was done. Twenty four pigs age 8-12 weeks were used in this study using pre-test and post-test groupexperimental design. The pigs were randomly divided into four groups : a group of pigs with no treatmentas a control (P0) and other three groups were given 1 g/kg body weigh (P1), 3 g/kg bw (P2) during threeconsecutive days and treated with 12.5% zodalben oral 0.5 mg of albendazole (P3). The number of A.suumand its eggs per gram (EPG) were identified by using international harmonization and anthelminticefficacy guideline. Then follow by observation and calculation of EPG, fecal egg count reduction (FECR),and the efficacy of the powder for seven days. The result showed there was a significant lower number ofA.suum infestation in the treated group of pigs compared to the control group as it was shown by theincrease of the efficacy against worms and the FECR value. However, there were different effects on theefficacy and FECR between the group that treated with 1 g/kg bw of the ripe papaya seed powder (efficacy75% and FECR 92.4%) compared to the groups that treated with 3 g/kg bw of the seed powder, and treatedwith zodalben 12.5% (both efficacy and FECR were 100%). It can be concluded that herbal powder of ripepapaya seed has a potential effect to be vermisidal, especially for A.suum.
Gambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali (CYTOLOGIC FIGURES OF PERITONEAL AND SYNOVIAL FLUIDS IN BALI DUCKS) Iwan Harjono Utama; Sri Kayati Widyastuti; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; Ida Bagus Oka Winaya; Ida Bagus Komang Ardana; Achoiro Wati Rasid; Tyas Pandieka Yoga
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to observe cellular composition and morphology in peritoneal and synovial fluids ofbali ducks. This study used 50 ducks, which were composed of 21 males and 29 females aged 8 to 12 weeks.After being declared clinically healthy, the sample ducks were slaughtered and peritoneal fluid specimenfrom the abdominal cavity and the synovial fluid of joints tibiotarsal were collected. Then, in preparationfor the specimen, each of fluid was smeared and stained with Giemsa. Observations were made under amicroscope with a magnification of 1000 times and observations expressed in the average number of cellsper field of view observation. The results showed (duck population that most of the observed sample): inthe peritoneal fluid there was 36% eosinophils observed and 38% had heterophile cells with 0-3 cells perfield of magnification 1000x view. Similarly, 28% ducks had lymphocytes with 13-16 cells, and as much as28% of ducks had macrophage cells with 5- 8 cells in observation . In the synovial fluid, however, 64%sample ducks had monocytes, while 72% having lymphocytes with 1-18 cells each of view field withmagnification 1000x. In addition, in the synovial fluid, as much as 62% of ducks also observed had 1 to 3cells heterophiles and 60% ducks had eosinophils (polimorphonuclear cells) per view field observation.Macrophage cells that were found in the peritoneal fluid were mainly hyper granulated. It can be concludedthat the amount of mononuclear cells in both types of body fluids is likely more dominant compared to thenumber of polymorphonuclear cells.
The Role of Cowmilk’s Colostrum as the Source of Passive Immunity and Growth Factor for Improving the Haematological Status and Titre of Antibody against Hog Cholera in Pre-Weaning Piglets Ida Bagus Komang Ardana
Journal of Global Pharma Technology Volume 11 Issue 08 (2019) Aug. 2019
Publisher : Journal of Global Pharma Technology

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Abstract

Background: pre-weaning piglets often suffer from anemia, and infected with the bacteria so as growth slows even death. Aim: The purpose of this study was to determine the effect of cow colostrum administration on hematology profile and anti-body titers as an immunomodulatory effect to prevent germ infection. Methods: Seventy two piglets were divided in 4 groups in this research. Group who were not given bovine colostrum as a control, group who were given cow colostrum dose of 3 ml per animal, groups of piglet given bovine colostrum dose of 6 ml per animal, and a group of bovine colostrum piglet given a dose of 9 ml per animal. At the age of 7 days vaccinated against Hog Cholera and repeated at 14 days. Besides, at the period of 20 days all the piglets to have blood drawn and then accommodated in a vacuum tube which already contains anticoagulant (EDTA) for examination hematological profile is checked with Automated Manual VET ABC Blood Counter, made in Germany. Finally at the age of 30 days all piglet blood drawn and then accommodated in the tube for examination Hog Cholera antibody titer by ELISA. The data were analyzed with statistical test. Result: The results showed bovine colostrum supplementation at a dose of 1-5 days of age piglet 3-9 ml/piglet can raise the profile hematology and Hog cholera antibody titer. Conclusion: Addition of cowmilk’s colostrum in pre-weaning for the first five days of life could improve both the hematological profile and immunomodulatory set.Keywords: Piglet, Bovine colostrum, Hematology.
Co-Authors . Suriansyah Achoiro Wati Rasid Adiari, Komang Sri Akbar, Saiful Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Ngurah Subawa Anak Agung Sagung Kendran Andi Azhary Azmy, Andi Azhary Anjuman Zukhri Apsari, Ni Wayan Diah Aryitahlia, Ninis Astawa, I Ketut Astuti, Komang Regi Kusuma Astuti, Komang Tri Cahyanti, Putu Yunika Damara, Doni Djara, Devita Vanessa Sukmawati Domingas Pereira Dr. Iyus Akhmad Haris,M.Pd . DWI SURYANTO Gede Ari Krisna Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Handayani, Ida Ayu Lidya HILDA PRASETYO I Dewa Ketut Harya Putra I Gede Gilang Ikra Raditya I Gede Made Sunu Satwika Nur Agung I Gede Soma I Gusti Agung Gede Putra Pemayun I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Ketut Berata I Ketut Suada I Made Bakta I Made Damriyasa I Made Dwinata I Made Kerta Pratama I Made Merdana I Made Sukada I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Cahyadi Putra, I Putu Cahyadi I Putu Sampurna I Wayan Masa Tenaya, I Wayan Masa I Wayan Sudira Ida Ayu Pasti Apsari Ida Bagus Dimas Kusumadarma Ida Bagus Kade Suardana Ida Bagus Oka Winaya Irmawan, Adi Ita Octarina Purba Iwan Harjono Utama Jamhari Jamhari Jayanata, I Made Jayantara, I Kadek Eka K. K. Agustina Kadek Karang Agustina Ketut Budiasa Ketut ELI Supartika KETUT SUADA Ketut Tono Pasek Gelgel Kiki Lulu Maduma Simanungkalit Kusamadarma, Ida Bagus Agung Dimas Lubis, Barata Sultan Luh Dewi Anggreni Luh Made Sudimartini LULUK WULANDARI Made Damriyasa, Made MADE KOTA BUDIASA Made Suma Anthara Made Suma Anthara Mahendra, I Putu Gede Marshanindya, Andra Meidianthi, Kadek Putri Mufa, Romy Muhammad Dary Muhammad Ainun Najib, Muhammad Ainun Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Kartini Ni Made Dwi Adnyana Pertiwi NUR KOMALASARI Nyoman Suartha Pio, Paulus Oktavianus Pradnyani, Gusti Ayu Putu Indira PRATIWI DEVI GM Pratiwi, Ni Made Diah Kusuma Putu Diah Puspa Adhi Putu Suastika Qutrotu'ain, Salsabila Robby Deddy Septianto Roby Rohmandhani Rosni Lumban Gaol S Siswanto Santoso, Sri Wahyuningsih Heri SARI PUTRIANI Septiyan, Fayyadh Syafiq Sidabutar, Febyana Sri Kayati Widyastuti Sri Rezeki Tampubolon Suharsono, Hamong Sukada, Made Sunusi, Sulham TRI KOMALA SARI Tyas Pandieka Yoga Wibawa, I Gede Pratama Candra WIDHI YANUNGRAH Widihantoro Gunawan Putra Wirata, Ketut Yoga Pratama Nuradi Yustika, I Made Aris