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PERTUMBUHAN ISOLAT JAMUR PASCAPANEN PENYEBAB BUSUK BUAH PISANG AMBON (Musa paradisiaca L.) SECARA IN VIVO rahmawati ibrahim saleh rahmawati; Rina agus setiawati; Elvi Rusmiyanto Pancaningwardoyo
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.11083

Abstract

Abstrak Permukaan kulit buah pisang ambon yang terluka dapat menyebabkan infeksi oleh jamur busuk buah. Penelitian ini bertujuan untuk mengetahui pertumbuhan jamur penyebab busuk buah pisang ambon. Penelitian dilaksanakan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura Pontianak. Metode yang digunakan meliputi isolasi jamur dari buah pisang ambon yang busuk dan inokulasi jamur pada buah pisang ambon yang sehat metode tanam langsung (Direct Plating Method). Parameter pengamatan meliputi pengukuran diameter pertumbuhan jamur dan pengukuran ketebalan kerusakan daging buah. Isolat jamur yang diperoleh ada 9 isolat dengan kode isolat PA1, PA2, PA3, PA4, PA5, PA6, PA7, PA8 dan PA9.Berdasarkan hasil pengamatan,diameter pertumbuhan terbesar berturut-turut adalah isolat PA 8, isolat PA 1,PA 4, PA 2, PA 7, PA 9, PA 5 dan PA 6 masing-masing 34,55 mm, 30,61 mm, 27,86 mm, 26,8 mm, 26,45 mm, 24,12 mm, 21,86 mm dan 21,34 mm, dengan kerusakan daging buah masing-masing 100%, sedangkan isolatPA 3 10,32 mm dengan kerusakan daging buah terendah yaitu 15%. Hal ini menunjukkan bahwa pisang ambon dapat terkontaminasi oleh berbagai koloni jamur dengan pertumbuhan berbeda-beda. Kata kunci : Diameter Pertumbuhan, Jamur, Kerusakan, Pisang Ambon
THE EFFECT OF MUNDU FRUIT (Garcinia dulcis (ROXB.) KURZ) METHANOL EXTRACT ON LYPHOPROTEIN PROFILE AND TRYGLISERIDE WHITE RAT (Rattus norvegicus L) Arum Setiawan; Laila Hanum; Elvi Rusmiyanto Pancaning Wardoyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.291 KB) | DOI: 10.23869/124

Abstract

Garcinia dulcis (Roxb.) Kurz) (Guttiferae) is a tropical fruit tree native to Indonesia and its known as an Asian medicinal plant used in folk medicines. In ethno pharmacology, Garcinia dulcis has been used in traditional medicine, such as anti-inflammatory, anti-tumor, anti-malaria and anti-bacterial. The scientific studies of biological activity of Garcinia dulcis on lipide profile and trigliseride are relatively measly. Therefore, the objectives of this research were to know the effect of methanol extract of Garcinia dulcis fruit on total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride rat hyperlipidemic. These studies using Garcinia dulcis fruit from Banyuasin, South Sumatra. In this observation, 30 male rat from Laboratory and Research Institute for Integrated Testing, Gadjah Mada University, 2 months old were used and divided into five groups randomly. The first group was given high cholesterol and lipid diet, as a normal group. The second group was given high cholesterol, lipid diet and simvastatin 3,6 mg/kg bow/day dosage, as a simvastatin group. The third, fourth and fifth groups was given high cholesterol, lipid diet and methanol extract 1.8; 2.7 and 3.6 mg/kg bow/day dosage. Total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride contains using spectrophotometry method, were observed 1 and 30 days. The differences among groups were analyzed using Anova followed by LSD to locate the differences. LSD analysis showed that Garcinia dulcis methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers total cholesterol levels highest in the amount of 32.17 mg/dL and 24.1 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw. Garcinia dulcis fruit methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers triglyceride levels by 7.22 mg/dL and 24.58 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw and controls. Garcinia dulcis fruit extract dose of 3.6 mg/dL lower low density lipoprotein-cholesterol highest of 16.45 mg/dL. Garcinia dulcis fruit methanol extract dose of 3.6 mg/kg bw was also able to increase the levels of high density lipoprotein-cholesterol by 5.62 mg/dL. The results showed that the fruit of Garcinia dulcis contain compounds that have potential as agents antihiperlipidemia and presumably through inhibition of the enzyme HMG-CoA reductase.
Anti-inflammatory Activity of Bajakah Stem (Spatholobus littoralis Hassk.) Ethanolic Extract in Carrageenan-Induced Paw Edema Mice Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo; Siti Ifadatin
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14126

Abstract

Bajakah tampala plant (Spatholobus littoralis Hassk.) has been used for traditional medicine by the Kalimantan Dayak tribe in the treatment of cancer. This study aimed to find out the potential of ethanol extract of the stem of Bajakah tampala (Spatholobus littoralis Hassk.) as an anti-inflammatory agent in carrageenan-induced mice. The method used was a Completely Randomized Design with five treatments and five replications. The treatments consisted of a water-treated mouse as a negative control, sodium diclofenac 30,8 mg kg-1 as a positive control, and an extract of bajakah dose 2.5; 250; 1250 mg kg-1 BW. The dose of bajakah extract was given orally one hour before carrageenan induction. The inflammation induction used the Winter method by injecting 0.15 ml of 2% carrageenan suspension into the soles of mice. The diameter of edema was measured for 210 minutes. The results showed that the diameter of the mice soles showed a significant increase in all groups after the carrageenan injection. The value of AUC (Area Under the Curve) of bajakah extract dose (2.5; 250; 1250 mg kg-1) from the 150th minute to the 210th minute did not show significant differences from one another. The sodium diclofenac as positive control gave the lowest total AUC value of 236 mm.minute, followed by bajakah extract at a dose of 2.5 mg kg-1 BW of 239.22 mm.minute. The calculation results of the percentage of the inflammation inhibition showed that the administration of ethanol extract of the stem of Bajakah, Spatholobus littoralis, at the lowest dose of 2.5 mg kg-1 BW provide the best inflammation inhibition with 19.21% inhibitory value, nearly approaching the positive control of diclofenac sodium with an inhibitory value of 21.53%.
Isolasi dan Karakterisasi Senyawa Kimia dari Kulit Batang Manggis (Garcinia mangostana Linn) Isolation and Characterization of Chemical Compound from Bark of Mangosteen (Garcinia mangostana Linn) Lia Destiarti; Ari Widiyantoro; Elvi Rusmiyanto; Maryati Maryati; Harlia Harlia; Ratu Safitri; Unang Supratman
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 7, No 2 (2009): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.098 KB) | DOI: 10.51850/jitkt.v7i2.231

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The purpose of this research is to isolate and characterize chemical compound from bark of mangosteen. Bark of mangosteen was macerated with methanol. The extract from that process was fractionated with n-hexane, methylen chloride, and ethyl acetate. Ethyl acetate fraction was separated and purified by vacuum column chromatography, gravitation column chromatography, and preparative thin layer chromatography. The relative pure compound was afforded from ethyl acetate fraction of mangosteen bark (8.5 mg) resulting from 1.5 kg of mangosteen bark. The yellow amorphous powder of compound melts at 114 ~ 116ºC (uncorrected). The purity of compounds was tested by 1 and 2 dimension thin layer chromatography which gave one spot on TLC plate. The ultraviolet-visible (in CH3OH solvent) spectrum showed absorption maximum at 318 nm (sinamoyl group/band I), 258 (shoulder), and 243 nm (benzoyl group/band II). Addition of NaOH caused batochromic shift of band I and band II predicted as two hydroxyl at C-4’ and C-7, respectively. The infrared spectrum displayed absorption bands of OH stretching at  3436 cm-1, C-H stretching at 2920 cm-1, C=O stretching at 1631 cm-1, C-O-C stretching at 1094 cm-1, and C-H aromatics bending at  970-800 cm-1. A molecular ion in the FABMS at m/z 271.36 [M+H] + was consistent for the molecular formula C15H10O5. The 1H NMR spectrum showed characteristic resonances of a flavone. Based on the result of phytochemical test and analysis of the spectrum, it is predicted that the compound belongs to flavone, a kind of flavonoids which has hydroxyl at C-5, C-7, and C-4.
KEANEKARAGAMAN GENETIK PADI LOKAL KALIMANTAN BARAT BERDASARKAN PENANDA ISOZIM Siti Ifadatin; Elvi Rusmiyanto PW
Floribunda Vol. 3 No. 1-2 (2006)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4354.137 KB) | DOI: 10.32556/floribunda.v3i1-2.2006.70

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Siti Ifadatin& Elvi Rusmiyanto PW. 2006. Genetic Diversity of Local Rice from West KalimantanBased on Isozyme Markers. Floribunda 3(2): 38-43.- A study on the genetic diversity has been conducted on eight cultivars of the local rice from West Kalimantan, namely Pulut Hitam, Pulut Melor, Pulut Sangai, Ringkak Serandah, Kak Cundong, Belait, Remunai and Payah Temakan. Isozyme analysis by using three Esterase Enzyme System (EST), malic dehydrogenase (MDH) and peroxidase (PER) resulted in 13 loci where 9 loci (69%) of them are polymorphic loci. Of the Esterase Enzyme System (EST), there were 2 band patterns (EST1 and EST2), in malic dehydrogenase enzyme system (MDH) there were 4 band patterns, while in the system of peroxidase (PER) there were 8 enzyme band patterns. In the analysis with three enzyme systems, there was no band pattern which is specific for a certain cultivar, so that they can be distinguished one and the other. The result of the clustering analysis on 8 local rice cultivars fromWest Kalimantan showed that all of the 8 cultivars were grouped into one group with similarity level 66%. The genetic similarity and the phylogeny of the rice could not be separated based on the origin. The result of this cluster analysis showed  a tendency that Padi Pulut became one group which is different from non Padi Pulut.
Deteksi dan Identifikasi Genera Bakteri Coliform Hasil Isolasi dari Minuman Lidah Buaya Dian Purnama Sari; Rahmawati Rahmawati; Elvi Rusmiyanto PW
Jurnal Labora Medika Vol 3, No 1 (2019): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.864 KB) | DOI: 10.26714/jlabmed.3.1.2019.29-35

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Coliform merupakan salah satu bakteri yang dapat menyebabkan penyakit pada manusia dan dijadikan sebagai bakteri indikator keberadaan bakteri patogen. Penelitian ini bertujuan untuk mendeteksi dan mengidentifikasi genera bakteri golongan Coliform dari sampel air minuman lidah buaya. Pengambilan sampel menggunakan metode random sampling.  Deteksi genera bakteri menggunakan media selektif dan identifikasi berdasarkan karakter morfologis dan biokimia. Hasil identifikasi diperoleh tiga genera bakteri golongan Coliform yakni anggota genus Escherichia, Klebsiella dan Citrobacter.
Optimization and Characterization of Wood Vinegar Produced by Shorea laevis Ridl Wood Pyrolysis Hasan Ashari Oramahi; Tsuyoshi Yoshimura; Elvi Rusmiyanto; Kustiati Kustiati
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.921 KB) | DOI: 10.22146/ijc.45783

Abstract

In this study, the Box-Behnken Design (BBD) was employed to investigate the effects of wood particle size, pyrolysis temperature, and pyrolysis time on the production of wood vinegar from Indonesia “bengkirai” wood (Shorea laevis Ridl). Characterization of wood vinegar was conducted by gas chromatography-mass spectrometry (GC-MS). Three variable designs consisting of wood particle size (2.00, 2.38, and 3.36 mm), pyrolysis temperature (350, 400, and 450 °C), and pyrolysis time (105, 120, and 135 min) were employed in a BBD response surface methodology (RSM-BBD). RSM-BBD results suggested that maximum wood vinegar yield would be obtained with a wood particle size of 3.85 mm, pyrolysis temperature of 400 °C, and pyrolysis time of 93 min. In addition, the mathematical model indicated the maximum wood vinegar yield would be 30.31%. The main compounds in wood vinegar were acetic acid, 1-hydroxy-2-propanone, furfural, 2,3-pentanedione, phenol, 2-methoxy phenol, 2-methoxy-4-methyl phenol, 2,6-dimethoxy phenol, and 1,2,4-trimethoxybenzene.
Aktivitas Hepatoprotektif Ekstrak Metanol Buah Lakum [Cayratia trifolia (L.) Domin] terhadap Hepatosit Tikus Putih (Rattus novergicus L.) yang Diinduksi Parasetamol Rizki Perdana Putri; Diah Wulandari Rousdy; Ari Hepi Yanti; Elvi Rusmiyanto Pancaning Wardoyo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2019.36.2.961

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Lakum [Cayratia trifolia (L.) Domin] dikenal akan kandungan metabolit sekundernya seperti flavonoid, terpenoid dan fenol. Kandungan metabolit sekunder ini mengindikasikan bahwa buah lakum berpotensi sebagai sumber antioksidan akibat overdosis parasetamol. Penelitian ini bertujuan untuk mengetahui aktivitas hepatoprotektif ekstrak metanol buah lakum pada hepar tikus putih Wistar yang diinduksi parasetamol dengan dosis tinggi. Buah lakum matang dari Kecamatan Sungai Kakap, Provinsi Kalimantan Barat diekstraksi melalui metode maserasi. Penelitian menggunakan Rancangan Acak Kelompok. Tiga puluh lima (35) tikus putih jantan galur Wistar (Rattus novergicus L.) dibagi ke dalam 7 kelompok perlakuan. Kelompok perlakuan terdiri dari 4 kelompok kontrol (normal, negatif, positif dan pelarut) dan 3 kelompok uji dari dosis ekstrak yang berbeda (115, 230 dan 345 mg kg-1). Parasetamol dan bahan uji lainnya diberikan secara oral masing-masing selama 7 hari. Hasil menunjukkan bahwa efek ekstrak metanol buah lakum dosis 230 mg kg-1 (hepatosit normal: 78,05 ± 0,92%; hepatosit nekrosis: 15,04 ± 0,80%) serupa dengan efek silymarin (hepatosit normal: 81,09 ± 0,93%; hepatosit degenerasi: 16,85 ± 0,15%) sebagai obat standar.  Dosis optimal ekstrak metanol C. trifolia yang berpotesi sebagai agen hepatoprotektif adalah 230 mg kg-1
Penggunaan Asap Cair dari Tandan Kosong Kelapa Sawit untuk Pengendalian Jamur Phytophthora citrophthora secara In Vitro Hasan Oramahi; Elvi Rusmiyanto; Kustiati Kustiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 38, No 1 (2021)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2021.38.1.1265

Abstract

Efficacy of wood vinegar produced from empty fruit bunches (EFB) against Phytophthora citrophthora fungus was evaluated. The objective of the present work is to to evaluate antifungal properties of wood vinegar from EFB against P. citrophthora fungus. Three kinds of wood vinegar were used three temperatures i.e. 350, 400 and 450 °C. Efficacy of wood vinegar from EFB for antifungal is a factorial 3 by 4 in a completely randomized design; the first factor was pyrolysis temperature of wood vinegar from EFB (350, 400, and 450 °C) and the second factor was treatment of concentration of wood vinegar from EFB  (0, 0.5, 1.0, and 1.5 %, v/v). The results showed that pyrolysis temperature effect of wood vinegar from EFB and concentration of wood vinegar were significantly different for inhibition of fungal growth. The highest inhibition was on wood vinegar for EFB with temperature pyrolysis of 450 °C and concentration of wood vinegar of 1% with average value of 100%.
RESPON PEMBERIAN PUPUK TRICHOKOMPOS KOTORAN BEBEK (Anas sp.) TERHADAP PERTUMBUHAN DAN PRODUKSI SAWI PAKCHOY (Brassica chinensis L.) PADA TANAH GAMBUT Abraham Sambo; Mukarlina Mukarlina; Elvi Rusmiyanto Pancaning Wardoyo
Jurnal AGROSAINS dan TEKNOLOGI Vol 7, No 1 (2022)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.7.1.13-24

Abstract

ABSTRAKPertumbuhan dan produksi sawi pakchoy (B. chinensis L.) pada tanah gambut di Kalimantan Barat masih rendah, salah satunya disebabkan pH tanah yang masam dan kurang tersedianya hara bagi tanaman. Penambahan pupuk trichokompos kotoran bebek diharapkan dapat meningkatkan pertumbuhan dan produksi sawi pakchoy. Penelitian ini bertujuan mengetahui pengaruh pupuk trichokompos kotoran bebek terhadap pertumbuhan dan produksi sawi pakchoy serta konsentrasi pupuk trichokompos kotoran bebek yang memberikan hasil terbaik untuk pertumbuhan sawi pakchoy. Penelitian menggunakan Rancangan Acak Lengkap dengan 5 taraf perlakuan yaitu A= 0 (Kontrol); B = 80 g/1,6 kg; C = 100 g/1,6 kg; D = 120 g/1,6 kg; dan E = 140 g/1,6 kg. Data dianalisis menggunakan ANOVA dan dilanjutkan uji Duncan dengan taraf kepercayaan 5%. Hasil penelitian menunjukkan bahwa pemberian pupuk trichokompos kotoran bebek berpengaruh nyata terhadap jumlah akar (helai), jumlah daun (helai), luas daun (cm2), berat basah total (g), dan berat kering total (g). Perlakuan pupuk trichokompos             140 g/1,6 kg adalah perlakuan yang memberikan hasil terbaik terhadap semua parameter pengamatan dengan jumlah akar 24,2 helai, jumlah daun 29,8 helai, luas daun 78,94 (cm2), berat basah total 244,7 g dan berat kering total 37,14 g.ABSTRACTThe growth and production of mustard pakchoy (B. chinensis L.) on peat soils in West Kalimantan is still low, one of which is due to the pH of sour soil and the lack of nutrient availability for plants. The addition of trichocompos fertilizer duck manure is expected to increase the growth and production of mustard pakchoy. This study aims to find out the influence of duck manure trichocompos fertilizer on the growth and production of mustard pakchoy and the concentration of trichokompos fertilizer duck manure which provides the best results for the growth of mustard pakchoy. The study used a Complete Random Design with 5 levels of treatment, namely A = 0 (Control); B = 80 grams / 1.6 kg; C = 100 grams / 1.6 kg; D = 120 grams / 1.6 kg; and E = 140 grams / 1.6 kg. The data was analyzed using ANOVA and continued duncan test with a confidence level of 5%. The results showed that the provision of trichocompos fertilizer duck manure had a real effect on the number of roots (strands), the number of leaves (strands), the area of the leaves (cm2), the total wet weight (grams), and the total dry weight (grams). Trichocompos fertilizer treatment of 140 grams / 1.6 kg is the treatment that gives the best results against all observation parameters with the number of roots 24.2 strands, the number of leaves 29.8 strands, the leaf area 78.94 (cm2), the total wet weight 244.7 grams and the total dry weight 37.14 grams.
Co-Authors Abraham Sambo Ahmad Yani Nelly Wahyuni Lia Destiarti Ali Djamhuri Andhini Aprilia Anggraini, Wiwit Ara, Chilliany Ari Hepi Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Widiyantoro Arini Miftahul Jannah Arum Setiawan Arum Setiawan Ary Kusmawati Azkiya, Tiara Catur Wulandari Catur Wulandari Chilliany Ara Cucu Suhery Desy Desy, Desy Devinda Ekarizky Diputri Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Dian Purnama Sari Dwi Gusmalawati Endah Sayekti Ester Yulinda Evi Noviani Fadhlullah, Hafizh Rizqi Firman Saputra Firman Saputra Fransisko, Satria Fuza Amalia Gusrizal Gusrizal Harlia Harlia Hasan Ashari Oramahi Hildayati, Umi Ibnur Rusi Indri Kusharyanti Irtiawati, Irtiawati Irwan Lovadi Junardi Junardi Kardina, Megawati Kustiati Kustiati Kustiati Laila Hanum Lena Dian Saputri Maryati Maryati Megawati - Mukarlina -, Mukarlina Mukarlina, Mukarlina Nurul Huda Oktavia, Vivi Pancaning Wardoyo Peli, Peli Perdana, Aurendyo Yuscel Rachmad Setijadi, Rachmad Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawati Ratu Safitri Renny Puspita Sari Ribka Sinta Uli Panjaitan Rikhsan Kurniatuhadi, Rikhsan Rina agus setiawati Riyandi Riyandi Riyandi Riyandi Riza Linda Riza Linda Rizki Perdana Putri Rousdy, Diah setiawati, Rina agus Sholihin, Nur Siti Hodijah Siti Ifadatin Siti Ifadatin Siti Khotimah Siti Nur Hasanah, Siti Nur Sulistrioningsih, Sulistrioningsih Sumi, Sumi Tri Rima Setyawati Tsuyoshi Yoshimura Turnip, Masnur Unang Supratman Widya Anggraeni Widya Anggraeni Wiwit Anggraini Yudha Arman Zulfa Zakiah