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AKTIVITAS ANTIBAKTERI EKSTRAK METANOL Acalypha hispida TERHADAP BAKTERI Shigella flexneri DAN Bacillus cereus IHB B 379 Elvi Rusmiyanto P. Wardoyo; Devinda Ekarizky Diputri; Rikhsan Kurniatuhadi
Tengkawang : Jurnal Ilmu Kehutanan Vol 10, No 2 (2020): Tengkawang : Jurnal Ilmu Kehutanan
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jt.v10i2.40187

Abstract

AbstractAcalpha hispida flower commonly used as traditional medicine. The antibacterial potential of the A. hispida flower can be used to treat diarrhea and cancer. The purpose of this study was to determine the antibacterial activity and effective concentration of methanol extract of A. hispida flower against to S. flexneri and B. cereus. Sample used was flower of A. hispida from Kota Pontianak. The solvent used is metahnol. Antibacterial activity test using the disc diffusion method (Kirby-Bauer) with parameters based on grading levels  extract concentration in S. flexneri was 1.2;  1.3;  1.4;  1.5;  and 1.6 g/ml and B. cereus for 0.75;  0.80;  0.85;  0.90;  and 0.95 g/mL, 10% DMSO and ciprofloaxin as a comparison. the results of the research showed that the concentration of methanol extract of A. hispida flower which can effectively inhibit the growth of S. flexneri and B. cereus bacteria at 1.2 g / mL and 0.75 g / mL. The methanol extract of A. hispida flower contains compounds of alkaloids, flavonoids, tannins, phenols, steroids and terpenoids.Keywords : Acalypha hispida, Antibacterial, Shigella flexneri, Bacillus cereus AbstrakAcalypha hispida merupakan tanaman obat trardisional yang sudah dimanfaatkan masyarakat secara turun temurun untuk mengobati diare dan kanker. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dan konsentrasi efektif ekstrak metanol bunga A. hispida terhadap bakteri Shigella flexneri dan Bacillus cereus. Sampel penelitian yang digunakan adalah bunga A. hispida yang diperoleh dari Kota Pontianak. Pelarut yang digunakan adalah methanol. Uji aktivitas antibakteri menggunakan metode difusi cakram (Kirby-Bauer) dengan perlakuan 5 taraf konsentrasi ekstrak pada bakteri S. flexneri sebesar 1,2; 1,3; 1,4; 1,5; dan 1,6 g/ml serta pada bakteri B. cereus IHB B 379 sebesar 0,75; 0,80; 0,85; 0,90; dan 0,95 g/mL, DMSO 10% serta sebagai pembanding digunakan ciprofloaxin. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak methanol bunga A. hispida yang secara efektif dapat menghambat pertumbuhan bakteri S. flexneri dan B. cereus sebesar 1,2 g/mL dan 0,75 g/mL. Ekstrak methanol bunga A. hispida mengandung senyawa golongan alkaloid, flavonoid, tannin, fenol, steroid dan terpenoid.Kata kunci: Acalypha hispida, antibakteri, Shigella flexneri, Bacillus cereus
Ethnobotany of Traditional Medicine Plant Ethnic Chinese in Pemangkat Sub-district, Sambas District Ribka Sinta Uli Panjaitan; Elvi Rusmiyanto P. W.; Mukarlina
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5658

Abstract

Plants used as medicine are believed to be effective in curing diseases. Traditional Chinese medicine has an influence on the growth of local traditional medicine. Traditional Chinese medicine are still practiced until now. This research aims to know the species and the family of plants used, as well as how the processing and methods of using Chinese ethnic medicinal plant in Pemangkat Sub-district from August to October 2022 in Pemangkat Sub-district. The research uses the participant observation. Selection of respondents using the snowball sampling method, with a total of 15 respondents. Data collection through semi-structured interviews. The results of this research included 28 species of plants from 15 families. The highest frequency sitations values are in kacangma/ginjean (Leonurus artemisia L.) and angkak/rice crops (Oryza sativa L.) with a point of 86%, the highest use value of species is kacangma/ginjean (Leonurus artemisia L.) with a point 0,6. The processing of plants is carried out in the six way, that is boiling, brewing, cooking, mashing, dipping and eating directly. Boiling being the most common processing with a value of 37%. Used medicinal plants in the six way, that is drinking, eating, dripping, washing and sticking. Drinking being the most used with a value of 61%.
Aktivitas Antibakteri Fraksi Etanol dan Etil Asestat Biji Sawo (Achras zapota L.) Terhadap Pertumbuhan Bakteri Shigella flexneri dan Bacillus cereus IHB B 379 Fuza Amalia; Elvi Rusmiyanto; Rahmawati
JURNAL BIOS LOGOS Vol. 14 No. 1 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i1.54052

Abstract

Tanaman sawo (Achras zapota L.) memiliki kandungan senyawa bioaktif berupa alkaloid, flavonoid, kuinon, saponin, steroid dan triterpenoid serta tanin yang dapat berperan sebagai antibakteri. Senyawa bioaktif tersebut dapat dimanfaatkan untuk mengatasi penyakit diare yang disebabkan oleh bakteri Shigella flexneri dan Bacillus cereus. Tujuan penelitian ini yaitu untuk menentukan aktivitas antibakteri dan konsentrasi terbaik fraksi etanol dan fraksi etil asetat biji sawo (A. zapota) terhadap pertumbuhan bakteri S. flexneri dan B. cereus. Biji sawo (A. zapota) yang digunakan berasal dari Desa Arang Limbung, Kubu Raya dan bakteri uji berasal dari koleksi Laboratorium Mikrobiologi FMIPA Universitas Tanjungpura. Uji aktivitas antibakteri menggunakan metode difusi cakram dengan parameter berdasarkan perbedaan tingkat konsentrasi fraksi 0,0025; 0,005; 0,01 g/mL serta DMSO 10% dan ciprofloaxin 500 g/mL digunakan sebagai pembanding. Cakram pada medium dibuat dengan diameter sebesar 6 mm, kemudian dimasukkan fraksi etanol dan fraksi etil asetat biji sawo (A. zapota) dan diinkubasi pada suhu 370C selama 24 jam. Hasil penelitian menunjukkan bahwa konsentrasi terbaik fraksi etanol biji sawo (A. zapota) dalam menghambat pertumbuhan S. flaxneri dan B. cereus adalah 0,005 g/mL dan 0,01 g/mL. Konsentrasi terbaik fraksi etil asetat biji sawo (A. zapota) dalam menghambat pertumbuhan S. flaxneri dan B. cereus adalah 0,0025 g/mL dan 0,01 g/mL. Kata Kunci: Achras zapota; antibakteri; Shigella flexneri; Bacillus cereus; biji sawo
Hubungan Kekerabatan Tanaman Talas (Colocasia esculenta (L.) Schott) di Kabupaten Bengkayang Berdasarkan Karakter Morfologi Chilliany Ara; Elvi Rusmiyanto Pancaning Wardoyo; Siti Ifadatin
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11129

Abstract

Taro (Colocasia esculenta (L.) Scott) is a plant classified as a tuber derived from the genus Colocasia and belongs to the Araceae tribe. Analysis of kinship relationships can be done by observing morphological characters. The purpose of this study was to determine the relationship between taro plants in Bengkayang Regency based on morphological characters. This research was conducted from April to June 2023. Taking 30 samples in 6 sub-districts using purposive sampling method. Analysis of kinship relationships using the NTSys program with the UPGMA method. The results showed that 30 taro accessions had a similarity coefficient value of 0.52-0.93 or 52-93%. The results of kinship relationship analysis obtained 4 clusters at a similarity coefficient value of 0.60 or 60%. The results of morphological characterization based on qualitative and quantitative characters show that taro accessions have diversity in leaf and cormus characters of taro, especially in color characters.
Pengaruh Ekstrak Metanol Buah Lakum (Cayratia trifolia L. Domin) terhadap Nilai SGOT dan SGPT Tikus Putih Diinduksi Parasetamol Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo; Catur Wulandari
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8530

Abstract

Lakum fruit (Cayratia trifolia L. (Domin)) has secondary metabolite compounds that are beneficial to health, one of which is a hepatoprotector. This study was conducted to prove the potential of hepatoprotective activity of lakum fruit methanol extract based on the value of SGPT and SGOT enzyme. The research method used a pre-posttest randomized control group design with a sample of 35 male rats divided into five treatment groups: solvent control group (CMC 0.5%); positive control group (HEPA-Q®); treatment group extract (0,575; 1,15; 1,725 mg/g BW). The treatment of induction of paracetamol and methanol extract of Cayratia trifolia fruit was carried out for 7 days respectively. The results showed that the dose of 1,15 mg/g BW of Cayratia trifolia fruit methanol extract had the most effective deterioration of SGOT and SGPT values with a percentage reduction of SGOT of 49.3% and SGPT of 51.8% (p <0.05) which approached control positive (HEPA-Q®). Based on the research conducted, methanol extract of Cayratia trifolia fruit has the potential to be hepatoprotective.
Efikasi Asap Cair Dari Kayu Mundu (Garcinia dulcis) Terhadap Penghambatan Pertumbuhan Jamur Aspergillus niger dan Aspergillus fumigatus Elvi Rusmiyanto Pancaning Wardoyo; Rikhsan Kurniatuhadi
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.30952

Abstract

AbstrakAspergillus merupakan genus jamur patogen yang menyerang pasca panen hasil tanaman hortikultura, seperti jagung dan kacang-kacangan. Pemanfaatan asap cair untuk mengendalikan pertumbuhan jamur patogen saat pasca panen merupakan salah satu alternatif yang alami dan aman untuk diterapkan. Tujuan penelitian ini adalah untuk menentukan kemampuan daya hambat dan kategori aktivitas antifungi asap cair berbahan dasar kayu mundu (Garcinia dulcis) terhadap jamur Aspergillus niger dan Aspergillus fumigatus serta kandungan senyawa kimianya. Kayu mundu yang digunakan berasal dari Kabupaten Kubu Raya. Media Potato Dextrose Agar (PDA) digunakan untuk uji aktivitas antifungi asap cair. Enam konsentrasi asap cair yaitu 0; 0,5; 1,0; 1,5, 2,0 dan 2,5% digunakan untuk uji dengan dithane M45 sebagai pembanding. Hasil penelitian menunjukkan bahwa asap cair berbahan dasar kayu mundu mampu menekan pertumbuhan jamur A. niger dan A. fumigatus secara signifikan (P <0,05). Konsentrasi asap cair berbahan dasar kayu mundu sebesar 2,5% memberikan hasil terbaik dengan nilai rata-rata penghambatan terhadap jamur A. niger dan A. fumigatus masing-masing sebesar 100 dan 91,81% dan tergolong kategori aktivitas sangat kuat. Uji kandungan senyawa kimia asap cair berbahan dasar kayu mundu mengandung 5 senyawa utama, yaitu 2-propanone, 1,2 ethanediol, acetid acid, phenol 2-methoxy-4-(2-propenyl)-(CAS) eugenol, dan 3-furaldehyde. Asap cair berbahan dasar kayu mundu berpotensi untuk dikembangkan sebagai pengendali serangan jamur patogen A. niger dan A. fumigatus pada pasca panen tanaman hortikultura.AbstractAspergillus is pathogenic fungi that infect post-harvest yields of horticultural crops, i.e., Zea mays and legum. Using liquid smoke is one of the natural and safe alternatives to control fungus and the growth of pathogenic fungi that attack post-harvest yields of cultivated plants. This study aimed to evaluate the antifungal properties of liquid smoke from mundu (Garcinia dulcis) wood against the fungus Aspergillus niger and Aspergillus fumigatus. Mundu wood is from Kubu Raya Regency. The Agar media used was Potato Dextrin Agar (PDA), and concentrations of liquid smoke were 0; 0.5; 1.0; 1.5; 2.0; and 2.5%. The results indicated that the liquid smoke from Mundu wood significantly inhibited the A. niger and A. fumigatus growth (P <0.05). The highest result of liquid smoke from Mundu wood against A. niger and A. fumigatus is a concentration of 2.5% with an average inhibition value of 100 and 91.81%, respectively, with a firm activity level. The contents of liquid smoke contained five dominant compounds, i.e., 2-propanone, 1,2 ethanediol, acetic acid, phenol 2-methoxy-4-(2-propenyl)-(CAS) eugenol, and 3-furaldehyde. Liquid smoke produced from mundu wood has the potential to be developed for the control of pathogenic fungi A. niger and A. fumigatus in post-harvest horticultural crops. 
Activities of Cayratia trifolia Fruit on Oxidative Stress and Histological Change in Physical Stress-Induced Mice Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning
Majalah Obat Tradisional Vol 30, No 1 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.93549

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Lakum fruit (Cayratia trifolia L. Domin) contains secondary metabolite compounds that have antioxidant properties. C trifolia belongs to the Vitaceae family, known as tropical grapes that have a sweet and itchy taste. This study aims to determine the potential of C. trifolia fruit methanol fraction as an antioxidant based on the parameters of malondialdehyde levels, superoxide dismutase enzymes, SGOT enzymes, SGPT enzymes, kidney and liver histology. The study used 24 male Swiss mice which were divided into 6 treatments, namely normal control, negative control, positive control (vitamin E), and C. trifolia methanol fraction 115; 230; 460 mg/kgBW. Physical stress induction was given in the form of fasting and swimming for 5 days. The data were analyzed by one-way ANOVA and descriptive analysis for histological parameters. The results showed that the methanol fraction of 115 mg/kg BW decreased the best levels of malondialdehyde serum (38 µM), SGOT (34.7 U/L) and SGPT (34.6 U/L) compared to other doses. Doses of 115 mg/kgBW also provided the best levels of the superoxide dismutase enzymes (1.73 U/L) almost equal to vitamin E as positive control. Observations of kidney and liver histology showed a decrease in damage to hepatocytes and renal glomerulus in the 115 mg/kgBW C. trifolia methanol fraction. This research contributes to the development of C. trifolia as a natural antioxidant.
Acute Toxicity of Bajakah Tampala (Spatholobus littoralis Hassk) Ethanolic Extract on The Microanatomy of Rat Spleens Arini Miftahul Jannah; Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.33728

Abstract

The utilization of bajakah tampala (Spatholobus littoralis Hassk) as a herbal medicine has been passed down through generations within the community in Kalimantan. However, no comprehensive studies have investigated the effects of consuming this herbal medicine without a standardized safe dose. This study aimed to determine the spleen microanatomy after administration of an acute dose of S. littoralis stem ethanolic extract. The stems of S. littoralis utilized in this study were obtained from the Sungai Ambawang Forest in Kubu Raya, West Kalimantan. These stems underwent maceration with 96% ethanol solvent. This study used a completely random design with four treatments, which included distilled water and S. littoralis stem ethanolic extract at three doses: 300; 2000; and 5000 mg/kg BW. Each treatment had five replicates. The results showed that the administration of extract doses above 2000 mg/kg BW caused a significant decrease in the diameter of the splenic white pulp, and the spleen’s microanatomy revealed that the boundary between the periarteriolar lymphoid sheaths (PALS) and the lymphoid follicles disappeared, as well as lymphoid follicles that shrank in size. This shows that the administration of extract doses above 2000 mg/kg BW causes symptoms of damage to the spleen microanatomy of experimental animals.
The Growth of Lemna Minor and Pistia Stratiotes in Batulayang Landfill’s Leachate Perdana, Aurendyo Yuscel; Zakiah, Zulfa; Rusmiyanto, Elvi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9513

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Lemna minor and Pistia stratiotes are plants that can be used to degrade haevy metal pollutants iron (Fe) and lead (Pb) present in aquatic environments and can imprave water quality. The purporse of this study was to determine the best combination between L. minor and P. stratiotes in improving leachate water quality. This study uses a Completely Randomized Design (CRD) with five treatment levels with 3 replications each. The data obtained were then analyzed using descriptive.The results showed that the best plant combination in improving leachate water quality and capable of absorbing heavy metals iron and lead was L1P3 with a percentage reduction of heavy metals iron and lead of 98,2% and 85 % respectively as a result L. minor and P. stratiotes after treatment on Batu Layang Landfill leachate experienced an increase in biomass and chlorophyll content, as well as being able to reduce lead and iron levels. The results of this study are in line with Minister of Environment Regulation of the Republic of Indonesia Number 59 of 2016 concerning quality standards for leachate from business premises or final disposal sites.
KIDNEY MICROANATOMY OF WHITE RATS ON THE ADMINISTRATION OF ETHANOL EXTRACT OF Spatholobus littoralis STEM ACUTE DOSE Rousdy, Diah; Azkiya, Tiara; Wardoyo, Elvi Rusmiyanto Pancaning
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3743

Abstract

Tampala bajakah stem (Spatholobus littoralis Hassk) is empirically used by the Indonesian people to recover from disease and maintain health, but the use of tampala bajakah has not been tested for doses that are safe for consumption. The purpose of this study was to determine the type of damage caused by acute doses of bajakah tampala stem extract on white rat kidney microanatomy (Rattus norvegicus Berkenhout). This study used bajakah stem derived from Ambawang, extraction using ethanol solvent, and Wistar strain white rat (± 150g) test as a test animal. The method used a Complete Randomized Design with 4 treatments namely normal control using distilled water, treatment of doses of 300, 2000, and 5000 mg/kg BW of bajakah tampala stem extract. Each group was given 5 replicates. The extract was shown in a single dose orally and observations were made for 14 days. The results obtained that doses of 2000 and 5000 mg/kg BW caused damage to white rat kidney tissue in the form of microanatomy dilatation of tubules and loss of the brush border, and doses of 300, 2000, and 5000 mg/kg BW caused necrosis and haemorrhage. The most severe damage to kidney microanatomy is the dose of 2000 and 5000 mg/kg BW. The administration of acute doses of bajakah extract has the potential to cause damage to kidney tissue.
Co-Authors Abraham Sambo Ahmad Yani Nelly Wahyuni Lia Destiarti Ali Djamhuri Andhini Aprilia Anggraini, Wiwit Ara, Chilliany Ari Hepi Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Widiyantoro Arini Miftahul Jannah Arum Setiawan Arum Setiawan Ary Kusmawati Azkiya, Tiara Catur Wulandari Catur Wulandari Chilliany Ara Cucu Suhery Desy Desy, Desy Devinda Ekarizky Diputri Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Diah Wulandari Rousdy Dian Purnama Sari Dwi Gusmalawati Endah Sayekti Ester Yulinda Evi Noviani Fadhlullah, Hafizh Rizqi Firman Saputra Firman Saputra Fransisko, Satria Fuza Amalia Gusrizal Gusrizal Harlia Harlia Hasan Ashari Oramahi Hildayati, Umi Ibnur Rusi Indri Kusharyanti Irtiawati, Irtiawati Irwan Lovadi Junardi Junardi Kardina, Megawati Kustiati Kustiati Kustiati Laila Hanum Lena Dian Saputri Maryati Maryati Megawati - Mukarlina -, Mukarlina Mukarlina, Mukarlina Nurul Huda Oktavia, Vivi Pancaning Wardoyo Peli, Peli Perdana, Aurendyo Yuscel Rachmad Setijadi, Rachmad Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawati Ratu Safitri Renny Puspita Sari Ribka Sinta Uli Panjaitan Rikhsan Kurniatuhadi, Rikhsan Rina agus setiawati Riyandi Riyandi Riyandi Riyandi Riza Linda Riza Linda Rizki Perdana Putri Rousdy, Diah setiawati, Rina agus Sholihin, Nur Siti Hodijah Siti Ifadatin Siti Ifadatin Siti Khotimah Siti Nur Hasanah, Siti Nur Sulistrioningsih, Sulistrioningsih Sumi, Sumi Tri Rima Setyawati Tsuyoshi Yoshimura Turnip, Masnur Unang Supratman Widya Anggraeni Widya Anggraeni Wiwit Anggraini Yudha Arman Zulfa Zakiah