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KORELASI PEMERIKSAAN IGE TOTAL DENGAN PERSENTASE EOSINOFIL PADA PASIEN TERDIAGNOSA HIPERSENSITIVITAS TIPE 1 : CORRELATION TOTAL IGE LEVELS AND EOSINOPHIL PERCENTAGE IN PATIENTS DIAGNOSED WITH TYPE 1 HYPERSENSITIVITY Fahri, Muhamad; Prihatiningsih, Diah; Sari, Lia Cahya
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 14 No. 5 (2025): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

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Abstract

Hipersensitivitas tipe 1 merupakan reaksi imunologis kompleks yang umumnya ditandai oleh peningkatan kadar Imunoglobulin E (IgE) dan aktivasi eosinofil, namun literatur yang ada menunjukkan inkonsistensi mengenai hubungan linier antara kedua parameter biologis tersebut. Penelitian ini bertujuan untuk mengevaluasi korelasi antara kadar IgE total dengan persentase eosinofil pada populasi pasien yang terdiagnosis hipersensitivitas tipe 1, guna memahami pola respons imun yang terjadi. Studi ini menggunakan desain observasional analitik dengan pendekatan cross-sectional, yang melibatkan 114 pasien yang memenuhi kriteria diagnosis klinis di lokasi penelitian. Berdasarkan hasil analisis statistik menggunakan uji Chi-Square, penelitian ini menemukan bahwa tidak terdapat korelasi yang signifikan antara kadar IgE total dengan persentase eosinofil (p = 0,241). Temuan ini mengindikasikan bahwa meskipun IgE dan eosinofil sama-sama berperan sentral dalam patogenesis alergi, keduanya mungkin merepresentasikan jalur aktivasi imun atau fase peradangan yang berbeda, di mana peningkatan kadar IgE tidak serta-merta disertai dengan eosinofilia perifer. Oleh karena itu, faktor lain seperti variasi individual, aktivasi sel mast, atau mediator inflamasi lainnya perlu dipertimbangkan, dan penegakan diagnosis sebaiknya tidak bergantung pada parameter tunggal melainkan melalui pendekatan multiparameter yang komprehensif. Kata Kunci: Eosinofil, hipersensitivitas tipe 1, IgE total Abstract Type 1 hypersensitivity is a complex immune reaction typically characterized by elevated levels of Immunoglobulin E (IgE) and eosinophil activation; however, existing literature presents inconsistent findings regarding the linear relationship between these two biological markers. This study aimed to evaluate the correlation between total IgE levels and eosinophil percentage in patients diagnosed with type 1 hypersensitivity to elucidate the underlying immune response patterns. Employing an analytical observational design with a cross-sectional approach, this study involved 114 patients who met the specific diagnostic criteria at the study site. Statistical analysis using the Chi-Square test revealed no significant correlation between total IgE levels and eosinophil percentage (p = 0.241). These findings indicate that although both IgE and eosinophils play central roles in allergic pathogenesis, they may represent distinct immune activation pathways or different inflammatory phases, where elevated IgE levels are not necessarily accompanied by peripheral eosinophilia. Consequently, other factors such as individual variability, mast cell activation, or alternative inflammatory mediators should be considered, suggesting that clinical diagnosis requires a comprehensive multiparameter approach rather than reliance on a single biomarker. Keywords: Eosinophils, Total IgE, Type 1 Hypersensitivity
Pengaruh Waktu Penundaan Pemeriksaan dan Suhu Penyimpanan Specimen Darah Edta terhadap Jumlah Trombosit dan Platelet Distribution Width (PDW) Putra, I Gusti Putu Agus Ferry Sutrisna; Prihatiningsih, Diah
Jurnal Kesehatan Terpadu Vol. 9 No. 2 (2025): Jurnal Kesehatan Terpadu
Publisher : LPPM Universitas Dhyana Pura

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Abstract

Pemeriksaan jumlah trombosit merupakan uji penyaring penting dalam membantu penegakan diagnosis. Namun, penundaan pemeriksaan darah utuh sering terjadi di berbagai fasilitas pelayanan kesehatan, sehingga dapat memengaruhi stabilitas sel darah. Penelitian ini bertujuan untuk mengetahui pengaruh waktu penundaan dan suhu penyimpanan terhadap jumlah trombosit dan Platelet Distribution Width (PDW). Penelitian ini merupakan eksperimen laboratorium dengan rancangan posttest only control group design. Sampel darah dari 20 responden dibagi menjadi enam kelompok perlakuan berdasarkan kombinasi waktu penundaan (segera, 1 jam, 3 jam, dan 6 jam) dan suhu penyimpanan (18–27 °C dan 2–8 °C). Data dianalisis menggunakan uji general linear model.Hasil menunjukkan bahwa suhu penyimpanan berpengaruh signifikan terhadap nilai trombosit dan PDW. Selain itu, interaksi antara waktu penundaan dan suhu juga menunjukkan perbedaan yang signifikan. Penyimpanan pada suhu ruang menyebabkan penurunan jumlah trombosit dan peningkatan PDW seiring lamanya waktu penundaan, sedangkan penyimpanan pada suhu 4–8 °C menunjukkan perubahan yang minimal. Temuan ini menegaskan bahwa waktu dan suhu penyimpanan berperan penting dalam menjaga keandalan hasil pemeriksaan trombosit dan PDW.
Pengaruh Waktu Penyimpanan Terhadap Hasil Pemeriksaan Kolesterol Total Pada Serum Segar Dan Serum Yang Disimpan Selama 72 Jam Pada Suhu 2-8°C Christina Natalia; Putu Ayu Parwati; Diah Prihatiningsih
TENTIRO: Jurnal Pendidikan dan Kesehatan Vol. 2 No. 2 (2025): November|| TENTIRO: Jurnal Pendidikan dan Kesehatan
Publisher : PT. GIAT KONSELING NUSANTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70420/tentiro.v2i2.212

Abstract

There are several factors that can affect sample stability such as contamination by bacteria and chemicals, exposure to sunlight, the influence of temperature and metabolism of living cells such as blood cells. So the storage method for blood samples is stored in serum form in a refrigerator with a temperature of 2-8°C, so that serum stability will last for 3-5 days. The purpose of this study was to determine the effect of storage time on the results of total cholesterol examinations in fresh serum and serum stored for 72 hours at a temperature of 2-8°C. The research method used was an experimental method with a cross-sectional approach a nd accidental sampling techniques. Data analysis used the Menn-Whitney test. The results of the study from 30 samples contained 24 samples (79.99%) of fresh serum total cholesterol levels at a temperature of 2-8°C in the normal category against serum sampl e examinations stored for 72 hours in the normal category, while 6 samples (20.01%) of fresh serum total cholesterol levels at a temperature of 2-8°C in the threshold category 1against serum sample examinations stored for 72 hours in the threshold category. The results of the data analysis showed a p-value of 0.000, which means that there is an effect of storage time on the results of total cholesterol examinations in fresh serum and serum stored for 72 hours at a temperature of 2-8°C. The factor that caused this was due to the delay in processing the sample, which resulted in an influence on the results of the study.
Pendidikan Remaja Sadar Test Anti Napza (PRESTASI): Edukasi dan Pendampingan: Adolescent Education on Anti-Drug Awareness Test (PRESTASI): Education and Mentoring Prihatiningsih, Diah; Bintari, Ni Wayan Desi; Widana, Anak Agung Gde Oka; Purwanti, Ika Setya
Jurnal Abdi Keperawatan dan Kedokteran Vol 5 No 1 (2026): Jurnal Abdi Kesehatan dan Kedokteran
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/jakk.v5i1.116

Abstract

Drug and addictive substance abuse (NAPZA) among adolescents continues to rise and brings serious consequences—ranging from physical and mental health problems to impaired social relationships and academic performance. In response to this issue, the community service program Pendidikan Remaja Sadar Tes Anti NAPZA (PRESTASI) was implemented at SMA PGRI 4 Denpasar to strengthen students’ knowledge, attitudes, and awareness regarding the dangers of NAPZA and the importance of early detection. This program employed a one-group pre-test–post-test design. A total of 30 students participated, with 10 students selected as peer educators. The activities included interactive lectures, group discussions, peer educator role-play simulations, and demonstrations on the use of rapid urine test kits. Changes in knowledge were measured using a 10-item multiple-choice questionnaire administered before and after the program. The results showed a clear improvement in knowledge: in the post-test, all participants (100%) achieved a “good” knowledge category, up from 26.67% in the pre-test. In addition, students demonstrated high enthusiasm during the rapid urine test demonstration session and expressed readiness to actively serve as peer educators in school-based prevention efforts. Overall, the PRESTASI program proved effective in improving students’ knowledge while strengthening the role of peer educators as health promotion agents within the school environment. Going forward, the program is recommended to be integrated into School Health Unit (UKS) activities and reinforced through collaboration with the National Narcotics Board (BNN) and other relevant stakeholders to ensure sustainable education and early detection initiatives.
Deteksi dini infeksi saluran kemih dan kesadaran napza di SMA PGRI 4 Denpasar Diah Prihatiningsih; Ni Wayan Desi Bintari; Ika Setya Purwanti; Anak Agung Gde Oka Widana
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 8, No 3 (2024): September
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v8i3.25919

Abstract

Abstrak Infeksi saluran kemih (ISK) adalah masalah kesehatan yang sering muncul akibat kebiasaan buruk dalam menjaga kebersihan diri, khususnya di area urogenital. Remaja berisiko lebih tinggi terkena ISK karena sering mengabaikan kebersihan pribadi. Selain itu, penyalahgunaan Napza (Narkotika, Psikotropika, dan Zat Adiktif lainnya) juga menjadi ancaman serius bagi remaja. Program Pengabdian kepada Masyarakat (PKM) di SMA PGRI 4 Denpasar bertujuan meningkatkan pengetahuan dan kesadaran remaja tentang pencegahan ISK dan bahaya Napza. Program ini bekerja sama dengan SMA PGRI 4 Denpasar sebagai mitra utama dalam kegiatan edukasi, sosialisasi, dan demonstrasi pemeriksaan ISK dan Napza. Hasil pre-test menunjukkan bahwa 33,33% siswa memiliki pengetahuan cukup dan 50% memiliki pengetahuan kurang tentang ISK, sementara 50% siswa memiliki pengetahuan cukup dan 16,67% memiliki pengetahuan kurang tentang Napza. Setelah kegiatan, post-test menunjukkan peningkatan signifikan: 91,67% siswa memiliki pengetahuan baik tentang ISK (peningkatan 116%) dan 100% memiliki pengetahuan baik tentang Napza (peningkatan 67%). Peningkatan ini menunjukkan efektivitas program PKM dalam meningkatkan pemahaman siswa mengenai ISK dan Napza. Peningkatan pengetahuan ini berbanding lurus dengan peningkatan kesadaran siswa, karena pemahaman yang lebih baik biasanya meningkatkan kesadaran dan mendorong perubahan perilaku positif. Kesadaran siswa tentang pentingnya menjaga kebersihan dan menghindari penyalahgunaan Napza meningkat sejalan dengan pengetahuan mereka yang lebih baik setelah mengikuti program ini. Implikasi dari program ini menekankan pentingnya edukasi berkelanjutan dan keterlibatan berbagai pihak dalam upaya pencegahan masalah kesehatan remaja, serta pentingnya dukungan dari keluarga dan masyarakat dalam menciptakan lingkungan yang mendukung kesehatan remaja.Kata kunci: pengetahuan; kesadaran; napza;  ISK; remaja Abstract Urinary tract infections (UTIs) are a common health issue often resulting from poor personal hygiene, especially in the urogenital area. Adolescents are at a higher risk of developing UTIs due to frequently neglecting personal hygiene. Additionally, substance abuse (narcotics, psychotropics, and other addictive substances) poses a serious threat to adolescents. The Community Service Program (PKM) at SMA PGRI 4 Denpasar aims to increase adolescents' knowledge and awareness of UTI prevention and the dangers of substance abuse. This program collaborates with SMA PGRI 4 Denpasar as the main partner in educational activities, socialization, and demonstrations of UTI and substance abuse screenings. Pre-test results showed that 33.33% of students had moderate knowledge and 50% had poor knowledge about UTIs, while 50% of students had moderate knowledge and 16.67% had poor knowledge about substance abuse. After the program, post-test results showed a significant improvement: 91.67% of students had good knowledge about UTIs (a 116% increase), and 100% had good knowledge about substance abuse (a 67% increase). This improvement indicates the effectiveness of the PKM program in enhancing students' understanding of UTIs and substance abuse. The increase in knowledge is directly proportional to an increase in students' awareness, as better understanding typically enhances awareness and encourages positive behavioral changes. Students' awareness of the importance of maintaining hygiene and avoiding substance abuse increased in line with their improved knowledge after participating in this program. The implications of this program emphasize the importance of continuous education and the involvement of various parties in preventing adolescent health issues, as well as the crucial role of family and community support in creating an environment that promotes adolescent health. Keywords: knowledge; awareness; substance abuse; UTIs; adolescents
Lead Levels in Blood and Urine, Blood Pressure, and Hemoglobin Levels in Online Motorcycle Taxi Workers in Denpasar Diah Prihatiningsih; Putu Ayu Parwati; Anak Agung Ayu Eka Cahyani
Jurnal Penelitian Pendidikan IPA Vol 12 No 3 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i3.13106

Abstract

This study aimed to analyze the relationship between blood lead (Pb) level, urine Pb level, blood pressure, and hemoglobin level among online motorcycle taxi workers in Denpasar. A quantitative cross-sectional design was used involving 96 drivers selected by purposive sampling. Data were collected through questionnaires, direct physical measurements, and laboratory examinations of blood Pb, urine Pb, and hemoglobin. Normality was tested using the Kolmogorov–Smirnov test. Because several variables were not normally distributed, the data were analyzed using Spearman rank correlation, Mann–Whitney U test, and linear regression. Blood Pb level was not significantly associated with systolic blood pressure, diastolic blood pressure, or hemoglobin level. Urine Pb level was not significantly associated with systolic or diastolic blood pressure, but showed a significant weak negative correlation with hemoglobin level. Blood Pb and urine Pb levels were not significantly correlated. Work duration was not significantly associated with Pb levels, whereas daily working hours showed a marginal association with blood Pb. These findings indicate limited hematological effects and support routine biomonitoring and preventive occupational health measures in traffic-exposed workers.
TINGKAT HEMOGLOBIN LANSIA: PERBEDAAN PENGUKURAN ANTARA DUA ALAT BERBEDA Diah Prihatiningsih; I Gusti Putu Agus Ferry Sutrisna Putra
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 1 No. 2 (2024): Vol. 1 No. 2 Edisi April 2024
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v1i2.105

Abstract

The examination of hemoglobin levels in the elderly is crucial as iron deficiency or anemia is often found in this age group. Various methods of hemoglobin examination are used in healthcare facilities. This study aims to identify the differences in hemoglobin levels using the Dirui BCC-3600 and Quick Check devices in the elderly population at Puskesmas Biromaru. This research is descriptive-comparative in nature. The sample consisted of 30 patients undergoing hemoglobin examination at the Puskesmas Biromaru laboratory between March 29th and April 30th, 2023. The sampling method used was accidental sampling, with venous blood samples from 30 respondents. The results of the study showed that the average hemoglobin level using the Dirui BCC-3600 device was 12.86 g/dL, while with the Quick Check device it was 11.77 g/dL. Normality testing was conducted using the Shapiro-Wilk test, which yielded a p-value of 0.023. A p-value < 0.05 indicates non-normal data distribution, thus followed by the Mann-Whitney test. The Mann-Whitney test resulted in a significant value (p) = 0.011. In conclusion, there is a significant difference in hemoglobin levels using the Dirui BCC-3600 and Quick Check devices in the elderly.
PENILAIAN HASIL CYSTATIN C PADA PASIEN GANGGUAN FUNGSI GINJAL DI LABORATORIUM PRODIA CIBUBUR Aminah Aminah; Putu Ayu Parwati; Moh. Adreng Pamungkas; Diah Prihatiningsih
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 1 No. 3 (2024): Vol. 1 No. 3 Edisi Juli 2024
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v1i3.171

Abstract

Kidney function disorders refer to a condition where the kidneys' ability to filter metabolic waste from the blood and excrete it through urine is compromised. Cystatin C serves as a sensitive and specific biomarker for measuring glomerular filtration rate, accurately reflecting kidney function. This study aims to evaluate Cystatin C levels based on gender among patients with kidney function disorders at the Prodia Cibubur clinic laboratory. The research was conducted from February to March 2023 using a descriptive cross-sectional approach. The sample comprised 120 patients with kidney function disorders selected through total sampling. The findings indicate that 16 patients (13.3%) had normal Cystatin C levels, while 104 patients (86.7%) had elevated Cystatin C levels. Notably, among males, 61 individuals (50.8%) tended to exhibit higher Cystatin C levels, suggesting a greater potential for kidney function disorders due to factors such as activity intensity, body mass influence, and hormonal factors.
ACCURACY OF TIMING IN TSH SAMPLE COLLECTION FOR CONGENITAL HYPOTHYROIDISM SCREENING AT PRIMA MEDIKA GENERAL HOSPITAL DENPASAR Gusti Ayu Kristalia Mustika Dewi; Didik Prasetya; Diah Prihatiningsih
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 2 No. 3 (2025): Vol. 2 No. 3 Edisi Juli 2025
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v2i3.979

Abstract

Congenital hypothyroidism (CH) is an endocrine disorder that can cause developmental delays if not detected and treated early. Congenital Hypothyroidism Screening (CHS) aims to detect abnormal levels of Thyroid Stimulating Hormone (TSH) in newborns before clinical symptoms appear. The timing of blood sample collection is a critical factor because TSH levels physiologically surge within the first 24 hours of life. This study aims to determine the accuracy of TSH sampling time in CH screening at Prima Medika General Hospital, Denpasar. A descriptive analytical method was used with a total sampling technique involving 105 neonates who underwent CHS from October to December 2024. Secondary data were obtained from laboratory request forms and the Laboratory Information System (LIS). Results showed that 92.4% of samples were collected on the second day of life, which aligns with the ideal window of 48–72 hours, and only 1.9% of neonates had elevated TSH levels. The study concludes that most sample collections met the recommended timing, contributing to valid results and effective CHS implementation. Timely sampling plays a vital role in early CH detection and reflects the overall quality of neonatal laboratory services.
The Relationship Between Pregnant Women's Knowledge of Triple Elimination Screening and Their Satisfaction with the Service Gusti Ayu Putu Ardianti Utami; Ni Luh Gede Puspita Yanti; Diah Prihatiningsih
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 2 (2026): April 2026
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i2.369

Abstract

Triple Elimination screening is a strategic effort to prevent the transmission of HIV, syphilis, and hepatitis B from mother to infant; however, its coverage and quality of implementation at primary healthcare facilities remain suboptimal. This study aimed to analyze the relationship between pregnant women’s knowledge of Triple Elimination screening and their level of satisfaction with services at UPTD Puskesmas Banjar II. An analytic correlational study with a cross-sectional design was conducted. The sample consisted of 70 first-trimester pregnant women selected using accidental sampling. Data were collected using structured questionnaires measuring knowledge levels and service satisfaction and were analyzed using the Chi-square test. The results showed that most respondents had a good level of knowledge (40%) and reported satisfaction with the services (62.9%). Bivariate analysis revealed a significant relationship between pregnant women’s knowledge and satisfaction with Triple Elimination screening services (p = 0.000). These findings indicate that improving pregnant women’s knowledge plays an important role in shaping positive perceptions of service quality. Therefore, optimizing health education is recommended to enhance service satisfaction and the success of the Triple Elimination program.
Co-Authors A.A Gde Oka Widana A.A Gde Oka Widana A.A Gde Oka Widana5 Oka Widana A.A. Gede Oka Widana AA Gde Oka Widana Abadi, Moh Fairuz Abadi, Moh. Fairuz Abdi Pertiwi, Dwi Liana Achmad Yudiarto Agus Ferry Sutrisna Putra, I Gusti Putu Aminah Aminah Anak Agung Ayu Eka Cahyani Anak Agung Ayu Eka Cahyani Anak Agung Oka Widana Andini, Ni Komang Sukra Anggraini, Desty Prastika Astika Livana Sani Cahyani, Anak Agung Ayu Eka Christina Natalia Dabi, Aberina Dewi, Luh Putu Diah Kusuma Didik Prasetya Dilisca Dwi Putri, Ni Luh Nova Essydhea Hasfika Fahri, Muhamad Gomes, Juvita Da Costa Gusti Ayu Kristalia Mustika Dewi Gusti Ayu Putu Ardianti Utami I Gst Pt Agus Ferry Sutrisna Putra I Gusti Ayu Agung Diah Dwi Sukma I Gusti Putu Agus Ferry Sutrisna I Gusti Putu Agus Ferry Sutrisna Putra I Gusti Putu Agus Ferry Sutrisna Putra I Gusti Putu Agus Ferry Sutrisna Putra I K. Putra Juliantara Ika Setya Purwanti Ika Yulfitrah James Sibarani Kariana, I Komang Ketut Indrawati Kirawan, I Komang Yoki Laksmi, I Gusti Ayu Putu Satya M. FAIRUZ ABADI, M. FAIRUZ Mirayanti, Ni Ketut Ayu Moh. Adreng Pamungkas Moh. Fairuz Abadi Nastiti, Herwin Rizky Ni Kadek Ayu Cintya Dewi Ni Komang Ayu Resiyanthi Ni Komang May Tri Arwindi Ni Komang Yuscika Devi Ni Luh Gede Puspita Yanti Ni Luh Gede Puspita Yanti Ni Luh Putu Devhy Ni Luh Putu Devhy Ni Luh Putu Devhy, Ni Luh Putu Ni Luh Putu Devhy, Ni Luh Putu Devhy Ni Made Puspawati Ni Made Putri Sumyarini Ni Made Widyanthi Ni Putu Nanik Haryani Ni Putu Rika Mudiarini Ni Wayan Desi Bintari Nopita Wati, Ni Made Nopita Wati, Ni Made Nuraini, Deswinda Fadhilah Nyoman Sudarma Nyoman Sudarma Pamungkas, Mohammad Adreng Parwati, Putu Ayu Prasetya, Didik Puspita Yanti, Ni Luh Gede Putri, Maylina Putri, Ni Luh Nova Dilisca Dwi Putu Ayu Parwati Putu Ayu Parwati Putu Gede Subhaktiyasa Putu Thrisna Dewi, Ni Luh Rastuti, Luh Putu Anik Santos, Silvino Dos Sari, Lia Cahya Sherly Novitasari Sri Idayani, Sri Sugyantari, Pande Putu Sutrisna Putra, I Gusti Putu Agus Ferry Timorensa, Katharina Novika Wangi, Ni Putu Ayu Cendana Wati, Ni Made Nopita Wati, Wayan Shinta Widana, A.A. G. Oka Widana, A.A.G. Oka Widana, Anak Agung Gde Oka Yanti, Ni Luh Gede Puspita