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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Current Trends in Aquatic Science International Journal of Interreligious and Intercultural Studies Jurnal Kedokteran Hewan
I Made Damriyasa
Faculty Of Veterinary Medicine, Udayana University
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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PEMBERANTASAN PENYAKIT PARASITER BERBASIS PEMERIKSAAN KOPROSKOPIS PADA KELOMPOK TERNAK SAPI KERTA NANDINI DESA PETANG DWINATA I M; I B M OKA; I M. DAMRIYASA
Buletin Udayana Mengabdi Vol 9 No 1 (2010): Volume 9 No.1 – April 2010
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT The prevalence of parasite infections of Bali cattle is high, and Bali cattle in Bali is one of the important livestocks. In order to improve the productivity of Bali cattle, control of parasitic diseases is important, especially for pigs raised traditionally in smallholder farmers in Bali. For affectivity of Parasite control in livestock, it should be based on laboratory evidence using coproscopical examination. The public service was conducted in Kelompok Ternak Kertanandini in Petang village of Badung regency. The objective of the activity was to control the parasite infections in Bali cattle based on coproscopical examination. Bali cattle were treated with commercial broad-spectrum antiparasitc drug. . Faecal samples were also collected and examined to identify the gastrointestinal parasite infections in cattle before the treatment. The activity contributes the social and economic wellbeing of smallholder households in this village.
SOSIALISASI PENYAKIT CACING PITA DAGING BABI DAN BEBERASAN PADA MASYARAKAT DI DESA BINAAN UNUD, DESA TIANYAR BARAT, KARANGASEM Dharmawan N.S; I M. Damriyasa; I. B.M. Oka; I K. K. Agustina; I. B. N. Swacita; I G. Mahardika
Buletin Udayana Mengabdi Vol 12 No 1 (2013): Volume 12 No.1 – April 2013
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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This Community Service was conducted on August 5th, 2012 in Tianyar Barat village, Karangasem regency inthe form of socialization of taeniosis and cysticercosis on the local community leaders. The aim of this programwas to give knowledge, clinical symptoms, transmission, and prevention of the taeniosis and cysticercosis. Therewere 37 participants who come from representatives of each area. Twenty-seven (72.9%) participants reportedthat at their house they raise pigs, and 11 out of 27 (40.7%) participants reported that the pigs are traditionallyrearing raised without a confinement fence. Free-range pig husbandry system was important risk factors for pigcysticercosis. The activity run smoothly as planned. Socialization of these zoonotic diseases has improved theunderstanding of all participants. At the end of the activity, all of them (100%) reported that they were satisfied;and all of them (100%) also are interested in becoming a volunteer to joint to inform the diseases to other people.
SOSIALISASI PENYAKIT RABIES PADA SISWA SLTA DI DESA BEBANDEM KARANGASEM Dharmawan. N. S.; I M. Damriyasa; I. B. K. Ardana; A. A. S. Kendran; K. K. Agustina
Buletin Udayana Mengabdi Vol 10 No 2 (2011): Volume 10 No.2 – September 2011
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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This Community Service was conducted on October 23rd. 2010 in Bebandem village, Karangasem regency in the form of socialization of rabies on senior high school students. The aim of this program was to give knowledge, clinical symptoms, transmission, and prevention of the rabies diseases. There were 50 participants who came from representatives of each class. Twenty-eight (56%) participants reported that at their house, they have dogs and cats, mostly (50%) have not been vaccinated. The activity runs smoothly and as planned. Socialization of rabies diseases has improved the understanding of all participants. At the end of the event, most of them (90%) reported that they were satisfied and there are 48 (96%) participants are interested in becoming a volunteer to join to inform rabies to other school children.
Profil Kimia Klinik Darah Sapi Bali (BLOOD CLINICAL CHEMISTRY PROFILES OF THE BALI CATTLE) Anak Agung Sagung Kendran; I Made Damriyasa; Nyoman Sadra Dharmawan; Ida Bagus Komang Ardana; Luh Dewi Anggreni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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For the evaluation of clinical tests in veterinary laboratories a reference basis of normal values ofclinically healthy farm animals is essential. It is well known that variables such as breed, age and sex havean influence on many blood parameters. The reference value of blood chemistry of bali cattle is scanty. Theaim of the current study was to evaluate blood chemistry of bali cattle includes alanine aminotransferase(ALT), aspartate aminotransferase (AST), urea, creatinine and glucose, measured by an auto analyzer.These data were used to establish reference intervals in bali cattle of different ages and sex. Blood wascollected from 195 (21 young males, 54 adult males, 60 young females, and 60 adult females) clinicallyhealthy bali cattle by means of jugular venipuncture. Values from different age and sex were comparedstatistically. The results of the study was obtained that ALT and blood glucose significantly higher inmales Bali cattle (P <0.05) then in females. ALT, AST and urea were significantly higher in adults Balicattle (P<0.05) then in young Bali cattle. However, glucose of young Bali cattle was higher when comparedwith the adult. There was no significant difference in the creatinine levels between adults and the youngones. The results of the current study provide reference intervals for clinical chemistry of Bali cattle
Seroprevalensi Bovine Cysticercosis pada Sapi Bali di Nusa Tenggara Barat, Indonesia (SEROPREVALENCE OF BOVINE CYSTICERCOSIS AT BALI CATTLE IN WEST NUSA TENGGARA, INDONESIA) Nyoman Sadra Dharmawan; I Made Damriyasa; I Gede Mahardika
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bovine cysticercosis is one of the animal and public health problems throughout the world. Besides this disease has a negative impact on public health, also causing economic losses for farmers due to heavy infected carcasses should be rejected. Until now the availability of data related to this parasitic disease, especially in the Nusa Tenggara region is very limited.The purpose of this study was to obtain information on the prevalence and distribution of bovine cysticercosis on bali cattle in West Nusa Tenggara, Indonesia. The study was conducted by examining of bali cattle sera obtained from the farmer by ELISA (Bio-X Diagnostics’s Cysticercosis Antigen ELISA Kit). The results of ELISA examination of 92 serum samples with cut off 0.295, shows that there are 4 positive sera (4.35%). Three positive sera are originated from Lombok and one positive sera from Sumbawa. These results indicate that Lombok Island and Sumbawa Island in West Nusa Tenggara are not free from bovine cysticercosis. Since C. bovis infection is zoonotic, studies of risk factors are necessary, as well as the availability of information about the estimated burden of disease and the economic loss of the disease. It is recommended that veterinarians be more accurate when conducting post mortum examination, especially on the cattle which comes from a positive area.
Protein Spesifik Cairan Kista Cysticercus bovis pada Sapi Bali yang Diinfeksi dengan Taenia saginata (SPECIFIC PROTEIN OF CYSTICERCUS BOVIS CYST FLUID ON BALI CATTLE EXPERIMENTALLY INFECTED WITH TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; Kadek Swastika; I Made Damriyasa; Ida Bagus Made Oka; I Nyoman Mantik Astawa
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercus bovis is the larval stage of Taenia saginata, the bovine tapeworm. The infection of thislarval in cattle musculature causes Bovine cysticercosis or Cysticercosis bovis.  Bovine cysticercosis is foundworldwide, but mostly in developing countries, where unhygienic conditions, poor cattle managementpractices, and the absence of meat inspection are common.  The adult Taenia infection in man is referredto as taeniasis.  Taenia saginata taeniasis is also found almost all over the world.  The prevalence ofTaenia saginata taeniasis has reported up to 27.5% in Gianyar Bali. In order to control the diseases,vaccination against the larvae stages in cattle of Taenia saginata may play an important role in controllingthe disease in the endemic regions.  The aims of the present study were to prepare and to investigate theimmunogenic protein as vaccine candidate for controlling  Cysticercus bovis infection in in Bali cattle.Cysticercus protein from the cyst fluid was firstly used to immunize mice and the mice sera were thencollected. Cysticercus proteins then analyzed using sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE).All cysticercus proteins were then visualized by Commasie blue staining. The proteins were also transferredonto nitrocellulose membrane and the immunogenic proteins were visualized by Western Blotting usingimmune sera raised in mice.  By Commasie blue staining, a total of 17 proteins were detected with themolecular weight of 14,86 kDa -122,40 kDa from the smallest to the largest. As many as 7 immunogenicproteins with the molecular weights of 16.81 kDa; 19.22 kDa; 20.98 kDa; 27.41 kDa; 34.02 kDa; 38.31 kDa;and 54.94kDa were detected.
Seroprevalensi dan Isolasi Toxoplasma gondii pada Ayam Kampung di Bali (SEROPREVALENCE AND ISOLATION OF TOXOPLASMA GONDII AMONG FREE-RANGE CHICKENS IN BALI) I Made Dwinata; Ida Bagus Made Oka; Nyoman Adi Suratma; I Made Damriyasa
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T.gondii oocysts in the environment and the meat of chicken is considered one of the sources of the humaninfection. A study to determine the seroprevalence of T.gondii in free ranging chickens in eight regency inBali have been undertaken. More over, attempt to isolate T gondii was also performed from the copropositivesample. Seroprevalence was detected using modified agglutination test (MAT) and isolation of T.gondiiwere performed from organs (heart and brain) using pepsin-HCl digestion method. Further the pathogenicityof the isolate was determined by bioassay using mice. The result showed that the seroprevalence was24.8% (31 out of 125 chickens examined). T.gondii was found in 17 of the 31 seropositive chickens (55%)more over all isolates were a vitulent to the mice.
Infeksi Cacing Pita pada Anjing Bali dan Gambaran Morfologinya (TAPE WORM INFECTION IN BALI DOGS AND THEIR MORPHOLOGICAL DESCRIPTION) Nyoman Sadra Dharmawan; Nyoman Adi Suratma; Made Damriyasa; I Made Merdana
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Experimental Infection of Taenia saginata eggs in Bali Cattle: Distribution and Density of Cysticercus bovis Nyoman Sadra Dharmawan; I Made Damriyasa; I Nengah Kapti; Putu Sutisna; Munehiro Okamoto; Akira Ito
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to observe the development, distribution, and infection density ofTaenia saginata metacestodes in Bali cattle. Three Bali cattle were experimentally infected with T. saginataeggs which were collected from taeniasis patients. The experimental animal was inoculated with : i)1000,00 T. saginata; ii) 500,000 eggs; and iii) 1,000,000 eggs, respectivelly 100,000 (cattle 1), 500,000(cattle 2), and 1,000,000 (cattle 3) T. saginata eggs, respectively. To observe the development of cysticerci,all cattle were slaughtered at 24 weeks post infection. To observe their distribution and density, slicingwas done to the cattle?s tissues. The study results showed that cysts were found distributed to all muscletissues and some visceral organs such as heart, diaphragm, lungs, and kidney of the cattle infected with100,000 and 500,000 T. saginata eggs. Density of the cyst was in the range of 11 to 95 cysts per 100 gramsof tissue. The highest density was noted in the heart (58/100 grams) and in diaphragm (55/100 grams).This study has confirmed that T. saginata eggs derived from taeniasis patient in Bali, if infected to Balicattle can develop and spread to all muscle tissues and some visceral organs. From this study it wasconcluded that it is necessary to include the heart in the meat inspection at slaughter house for possibilityof T. saginata cyst infection.$?
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Co-Authors Adryani Ris Akira Ito Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Anak Agung Sagung Kendran BERNA NATALIA SILABAN Desak Nyoman Dewi Indira Laksmi Dewa Putu Sahadewa Dharmawan N.S Dina Harumna Endang Wulandari Suryaningtyas Farika E. Y Gede Yudi Suryawan Handayani, Ni Made HERTATI ANRIANI LUBIS I Gede Mahardika I K. Berata I K. K. Agustina I Kadek Saka Wiryana I Kadek Swastika I Ketut Anom Dada I Ketut Berata I Ketut Suada I Made Angga Prayoga I Made Bakta I Made Dwinata I MADE INDRA PERMADI I Made Kardena I Made Merdana I Made Subrata I Nengah Kapti I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Sunita I Nyoman Wijaya Guna I PUTU YOGA LOKANTARA I Wayan Bebas I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan I. B. Putra-Manuaba Ida Ayu Laksmi Puspita Dewi Ida Ayu Pasti Apsari Ida Bagus Gde Yudha Triguna Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya K. K. Agustina Kadek Agus Agra Arnawa Kadek Ayu Dwi Suryastini Kadek Dianiyanti Kadek Karang Agustina Ketut Budiasa Luh Dewi Anggreni MARIANA KRESTY FERDINANDEZ MORI FRISKA TAMBA Muhammad Ulqiya Syukron Munehiro Okamoto N. T. Suryadhi N.S. Dharmawan Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Luh Ayu Trisnayanti Ni Luh Gede Merry Cintya Laksmi Ni Luh Gede Sudaryati Ni Made Yunik Novita Dewi Dewi Nyoman Tigeh Suryadi PANDE KETUT YULIARI PRATIWI DEVI GM Putu Nara Kusuma Prasanjaya Putu Suma Githa Sanjaya Putu Sutisna sang gede purnama Sri Hartini STEFAN THEISEN Stelen, I Dewa Ketut Raeyadi Purnama Sudiartawan, I Putu Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun, Tjok Gede Oka TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia