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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Current Trends in Aquatic Science International Journal of Interreligious and Intercultural Studies Jurnal Kedokteran Hewan
I Made Damriyasa
Faculty Of Veterinary Medicine, Udayana University
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Seroprevalensi Sistiserkosis pada Babi di Papua (SEROPREVALENCE OF PIG CYSTICERCOSIS IN PAPUA REGION) Ida Bagus Ngurah Swacita; I Ketut Suada; Ketut Budiasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; I Nyoman Polos; I Made Damriyasa
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.971 KB) | DOI: 10.19087/jveteriner.2017.18.1.18

Abstract

Pig cysticercosis is an infection caused by the larval stage of pork tapeworm and Papua is one of the largest endemic areas of cysticercosis in Indonesia. This survey aim was to determine the seroprevalence of pig cystisercosis in Papua. A total of 311 pig serum samples collected from six regencies in Papua were examined using Enzyme Linked Immunosorbent Assay (ELISA). The result of the survey showed that the average seroprevalence of pig cysticercosis in Papua was 23.5% (73/311), where the highest seroprevalence was found in the regency of Jayawijaya was 42.6% (43/101), Biak 22.5% (9/40), Nabire 20.6% (7/34), Mimika 17% (8/47), Jayapura 13.5% (5/37), and Merauke 1.9% (1/52). It can be concluded that the seroprevalence of pig cysticercosis in Papua is still high, therefore, it is necessary to do more intensive programs to prevent and control this disease. ABSTRAK Sistiserkosis pada babi adalah infeksi yang disebabkan oleh stadium larva cacing pita, dan Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Tujuan penelitian ini adalah untuk menentukan seroprevalensi sistiserkosis pada babi di Papua. Sebanyak 311 sampel serum babi yang dikumpulkan dari enam kabupaten di Papua diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa rataan seroprevalensi sistiserkosis pada babi di Papua sebesar 23,5% (73/311), dan seroprevalensi terbesar ditemukan di Kabupaten Jayawijaya 42,6% (43/101), Biak 22,5% (9/40), Nabire 20,6% (7/34), Mimika 17% (8/47), Jayapura 13,5% (5/37), dan Merauke 1,9% (1/52). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada babi di Papua masih tinggi, sehingga diperlukan program yang lebih intensif untuk mencegah dan mengontrol penyakit ini.
Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN) Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono .; I Made Damriyasa
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this study was to determine the presence of Toxoplasma gondii in free-rangechicken using Polymerase Chain Reaction (PCR) method based on the tachyzoite and bradyzoitestage specific genes. SAG1 and BAG1 are the tachyzoite and bradyzoite stage-specific gene respectively.The primers for SAG1 and BAG1 were designed using Web-base Program Primer 3. Genomic DNAfrom free-range chicken heart and brain was isolated using Pure-Link Genomic Isolation Kit.DNA amplification by PCR using primers for SAG1 and BAG1 genes was used for diagnosis ofT.gondii. The results showed that the DNA amplification using primers for SAG1 and BAG1 geneswas successfully applied to determine of Toxoplasma gondii in free-range chicken.
Kejadian Dermatosis yang Tinggi pada Anjing Jalanan di Bali (A HAIGH DERMATOSIS INCIDENCE AMONG STRAY DOGS IN BALI) I Kadek Saka Wiryana; I Made Damriyasa; Nyoman Sadra Dharmawan; Kadek Agus Agra Arnawa; Kadek Dianiyanti; Dina Harumna
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was designed to investigate the incidence of dermatosis among stray dogs in Bali. A totalof 401 stray dogs collected in the animal shelter of Bali Animal Welfare Association during 2011 wasinvestigated in this study. Dogs were examined by the clinical sign and continued by skin scraping, cytologyand wood lamp examination. In total, 37,9% sample were positive for dermatosis. Bacterial causeddermatosis found to be the most prevalent (23,6%), followed by tick and fleas (16.5%), scabies (12.7%),malassezia (8.2%), demodek (8%) and ringworm (4.5%) respectively. We also found that dermatosis weremore prevalent in male dogs (50.9%) rather than female dogs (32.9%). Dogs 9-12 weeks old were moreheavily infected (45.8%) than other group. In conclusion, the incidence of dermatosis among stray dogs inBali is relatively high. This may need serious awareness as some of this dermatosis were zoonotic tohuman.
Pemakaian Herbal Serbuk Biji Pepaya Matang dalam Pengendalian Infeksi Ascaris suum pada Babi Ida Bagus Komang Ardana; I Made Bakta; I Made Damriyasa
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study on vermisidal effect of ripe papaya seed powder against egg and worm of A.suum in landracepig was done. Twenty four pigs age 8-12 weeks were used in this study using pre-test and post-test groupexperimental design. The pigs were randomly divided into four groups : a group of pigs with no treatmentas a control (P0) and other three groups were given 1 g/kg body weigh (P1), 3 g/kg bw (P2) during threeconsecutive days and treated with 12.5% zodalben oral 0.5 mg of albendazole (P3). The number of A.suumand its eggs per gram (EPG) were identified by using international harmonization and anthelminticefficacy guideline. Then follow by observation and calculation of EPG, fecal egg count reduction (FECR),and the efficacy of the powder for seven days. The result showed there was a significant lower number ofA.suum infestation in the treated group of pigs compared to the control group as it was shown by theincrease of the efficacy against worms and the FECR value. However, there were different effects on theefficacy and FECR between the group that treated with 1 g/kg bw of the ripe papaya seed powder (efficacy75% and FECR 92.4%) compared to the groups that treated with 3 g/kg bw of the seed powder, and treatedwith zodalben 12.5% (both efficacy and FECR were 100%). It can be concluded that herbal powder of ripepapaya seed has a potential effect to be vermisidal, especially for A.suum.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono .; I Made Damriyasa
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.
Deteksi Antibodi dan Isolasi Toxoplasma gondii pada Itik lokal di Bali (DETECTION ANTIBODIES AND ISOLATION OF TOXOPLASMA GONDII IN DOMESTIC DUCK IN BALI ) Made Dwinata; Ida Bagus Made Oka; I Made Damriyasa
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to determine detection antibodies and isolation T. gondii infection in domestic duck in Bali. A total, 188 domestic ducks sera were examined using indirect haemaglutination test kit (IHA). Heart, brain and muscle of seropositive IHA test were used for isolation with pepsin-HCL digestion and bioassay in mice and cat. The result of these research showed that 47 (25%) ducks were found to be positive for T. gondii antibodies at the cut-off e” 1:64. The seroprevalence in male and female duck were 27,8% and 22,4% respectively, however, statistical analysis showed that the difference was not significant (P>0,05). The seroprevalence in cage and free-range duck were 18,7% and 29,2% respectively, but the difference was not statistically significant (P>0,05). The antibodies titer ranging from 1:64 to 1:2048. Also, viable T. gondii was isolated from seropositive duck by bioassay mice and cat. Most of the isolated strains were avirulent to mice. This study showed that domestic duck could have a potensial role in transmitting toxoplasmosis to human in Bali.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Co-Authors Adryani Ris Akira Ito Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Anak Agung Sagung Kendran BERNA NATALIA SILABAN Desak Nyoman Dewi Indira Laksmi Dewa Putu Sahadewa Dharmawan N.S Dina Harumna Endang Wulandari Suryaningtyas Farika E. Y Gede Yudi Suryawan Handayani, Ni Made HERTATI ANRIANI LUBIS I Gede Mahardika I K. Berata I K. K. Agustina I Kadek Saka Wiryana I Kadek Swastika I Ketut Anom Dada I Ketut Berata I Ketut Suada I Made Angga Prayoga I Made Bakta I Made Dwinata I MADE INDRA PERMADI I Made Kardena I Made Merdana I Made Subrata I Nengah Kapti I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Sunita I Nyoman Wijaya Guna I PUTU YOGA LOKANTARA I Wayan Bebas I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan I. B. Putra-Manuaba Ida Ayu Laksmi Puspita Dewi Ida Ayu Pasti Apsari Ida Bagus Gde Yudha Triguna Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya K. K. Agustina Kadek Agus Agra Arnawa Kadek Ayu Dwi Suryastini Kadek Dianiyanti Kadek Karang Agustina Ketut Budiasa Luh Dewi Anggreni MARIANA KRESTY FERDINANDEZ MORI FRISKA TAMBA Muhammad Ulqiya Syukron Munehiro Okamoto N. T. Suryadhi N.S. Dharmawan Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Luh Ayu Trisnayanti Ni Luh Gede Merry Cintya Laksmi Ni Luh Gede Sudaryati Ni Made Yunik Novita Dewi Dewi Nyoman Tigeh Suryadi PANDE KETUT YULIARI PRATIWI DEVI GM Putu Nara Kusuma Prasanjaya Putu Suma Githa Sanjaya Putu Sutisna sang gede purnama Sri Hartini STEFAN THEISEN Stelen, I Dewa Ketut Raeyadi Purnama Sudiartawan, I Putu Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun, Tjok Gede Oka TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia