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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Current Trends in Aquatic Science International Journal of Interreligious and Intercultural Studies Jurnal Kedokteran Hewan
I Made Damriyasa
Faculty Of Veterinary Medicine, Udayana University
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA) Ida Bagus Oka Winaya; I Nyoman Mantik Astawa; I Made Damriyasa; Nyoman Sadra Dharmawan; I Ketut Berata
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.
Endoparasit Pada Ikan Kakap Merah (Lutjanus sp.) di Pantai Kelan, Bali; Potensi Bersifat Zoonosis Sri Hartini; I Made Damriyasa; Endang Wulandari Suryaningtyas
Current Trends in Aquatic Science Vol 2 No 2 (2019)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

One of the factors that reduce fish production and population is disease. This study aims to determine the various types of zoonotic endoparasites, prevalence, intensity, abundance, diversity index, relationship of fish length-weight, relationship length with the intensity of parasites, and the relationship between weight and intensity of parasites. This research was conducted at Kelan Beach, Bali from December 2018 to April 2019. The research used a descriptive method using purposive sampling for data retrieval. Endoparasites were found in 9 types, namely Cucullanus sp. (3 ind), Anisakis sp. (7 ind), Lecithochirium sp. (1 ind), Hamacreadium lethrini (6 ind), Helicometra sp. (1 ind), Siphoderina sp (7 ind). Trypanorhyncha (32 ind), Tetraphylidea (4 ind), and Acanthocephalus sp. (1 ind). Organs infected by endoparasites were stomach (34%), liver (3%), and intestine (63%). The highest prevalence obtained was Trypanorhyncha (37.5%). The highest intensity obtained was Hamacreadium lethrini and Cucullanus sp. about 3 ind / fish. The highest abundance obtained was Trypanorhyncha (80%). The diversity index was 1,583. The length and weight relationship of Red Snapper (Lutjanus sp.) shows isometric relationship after t-test. Long relationship with parasite intensity has a very low relationship with a velue (r) of 0,142. The relationship between weight and parasite intensity has a very low relationship with a velue (r) of 0,139. Based on 9 types of endoparasites found 1 type is zoonotic namely Anisakis sp. that cause Anisakiasis disease.
Identifikasi dan Intensitas Rocinela sp. Pada Ikan Kerapu di Pantai Kedonganan, Jimbaran, Bali Ni Luh Ayu Trisnayanti; I Made Damriyasa; Endang Wulandari Suryaningtyas
Current Trends in Aquatic Science Vol 2 No 2 (2019)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

This research was aims to determine the intensity level of Rocinela sp. in groupers. Grouper fish samples were taken at Kedonganan Beach, Jimbaran, Bali. Grouper fish is a type of fish that lives around coral reefs, that have a high economic value. Grouper fish sampling was done randomly method, so that the length and weight of the grouper samples used will be more diverse. Fish samples obtained at Kedonganan Beach will be excamine for parasites at the Fisheries Laboratory, Marine and Fisheries Faculty, Udayana University. The grouper samples observed in this study were 40 individuals. The type of parasite found in grouper is Rocinela sp. This parasite belongs to the order of Isopoda which is a type of ectoparasite that attacks fish. Ectoparasites are a type of parasite that infects the outside of a fish's body, such as the gills, mouth, eyes, and operculum. Ectoparasites can cause damage and impaired function in the infected organ. Based on the results of the study found Rocinel sp. in 26 fish sampled with a total number of parasites of 28 individuals. Most of these parasites were found in the gills with a percentage of 92.6% and some were found in the operculum with a percentage of 7.4%. The intensity value of parasitic Rocinela sp. that is 1,067, this value indicates infection of Rocinela sp. which is low in grouper fish at Kedonganan Beach, Jimbaran, Bali.
PERAN OVISIDAL HERBAL SERBUK BIJI PEPAYA MATANG DAN ALBENDAZOL TERHADAP DAYA BEREMBRIO TELUR CACING Ascaris suum SECARA IN VIVO Ida Bagus Komang Ardana; I Made Bakta; I Made Damriyasa
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.967 KB) | DOI: 10.21157/j.ked.hewan.v6i1.353

Abstract

Telah dilakukan penelitian untuk mengetahui pemberian herbal serbuk biji pepaya matang secara oral pada babi ascariasis terhadap daya berembrio telur cacing pada tinja dan pada uterus cacing yang keluar dari babi yang diobati tersebut. Pada percobaan ini sebanyak 24 ekor babi Landrace betina dengan bobot badan ± 10-15 kg, umur ± 15 minggu yang terinfeksi cacing Ascaris suum (A. suum) secara alami, dengan egg per gram (EPG) berkisar 250-2500 butir. Babi tersebut dibagi menjadi empat kelompok perlakuan (P0, P1, P2, dan P3) masing-masing terdiri dari 6 ekor. Kelompok P1 dan kelompok P2 mendapat perlakuan dengan herbal serbuk biji pepaya matang per oral dengan dosis masing-masing 1 dan 3 g/kg bobot badan (P2) selama 3 hari berturut-turut. Kelompok P3 mendapat perlakuan dengan albendazol dosis 0,5 mg/kg bobot badan (Zodalben 0,04 ml/kg bobot badan) sedangkan kelompok P0 bertindak sebagai kontrol tanpa pengobatan. Hasil penelitian ini menunjukkan bahwa pengobatan dengan herbal serbuk biji pepaya matang pada babi ascariasis ternyata mampu menurunkan daya berembrio telur cacing dalam tinja babi dan dalam uterus cacing secara efektif sehingga dapat digunakan untuk pengendalian askariasis pada babi.
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP Ida Ayu Pasti Apsari; Wayan Tunas Artama; Sumartono s; I Made Damriyasa
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.127 KB) | DOI: 10.21157/j.ked.hewan.v7i1.579

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Hindu Religious Practices in Pregnancy Care for Stunting Prevention among Hindu’s Family in Kupang City Dewa Putu Sahadewa; Ida Bagus Gde Yudha Triguna; I Made Damriyasa
International Journal of Interreligious and Intercultural Studies Vol. 8 No. 1 (2025): International Journal of Interreligious and Intercultural Studies
Publisher : UNHI PRESS

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Abstract

This study examines the influence of Hindu religious practices on prenatal care and stunting prevention among Hindu families in Kupang City, East Nusa Tenggara. Despite the region's high stunting prevalence, Hindu families exhibit significantly lower rates. To understand this disparity, the research employs a qualitative methods approach, incorporating in-depth interviews, observations, and a textual analysis of relevant Hindu scriptures. By delving into specific practices like magedong-gedongan, this study aims to identify cultural factors contributing to improved maternal and child health outcomes. Hindu practices, such as magedong-gedongan, offer valuable prenatal education and socio-cultural bounding , while banten and prasadham provides nutritional guidance. Additionally, the ceremonial aspects and daily prayers contribute to optimal physical , psychological and mental well-being, essential for stunting prevention. The findings of this study may inform culturally sensitive public health interventions and programs to address stunting, especially in this region
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP Apsari, Ida Ayu Pasti; Artama, Wayan Tunas; s, Sumartono; Damriyasa, I Made
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.579

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. Themethod required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/l Sag1 probe and 0.58 pg/l Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/l Sag1 probe and 0.58 pg/l Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
PERAN OVISIDAL HERBAL SERBUK BIJI PEPAYA MATANG DAN ALBENDAZOL TERHADAP DAYA BEREMBRIO TELUR CACING Ascaris suum SECARA IN VIVO Ardana, Ida Bagus Komang; Bakta, I Made; Damriyasa, I Made
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.353

Abstract

Telah dilakukan penelitian untuk mengetahui pemberian herbal serbuk biji pepaya matang secara oral pada babi ascariasis terhadap dayaberembrio telur cacing pada tinja dan pada uterus cacing yang keluar dari babi yang diobati tersebut. Pada percobaan ini sebanyak 24 ekor babi Landrace betina dengan bobot badan 10-15 kg, umur 15 minggu yang terinfeksi cacing Ascaris suum (A. suum) secara alami, dengan egg per gram (EPG) berkisar 250-2500 butir. Babi tersebut dibagi menjadi empat kelompok perlakuan (P0, P1, P2, dan P3) masing-masing terdiri dari 6 ekor. Kelompok P1 dan kelompok P2 mendapat perlakuan dengan herbal serbuk biji pepaya matang per oral dengan dosis masing-masing 1 dan 3 g/kg bobot badan (P2) selama 3 hari berturut-turut. Kelompok P3 mendapat perlakuan dengan albendazol dosis 0,5 mg/kg bobot badan (Zodalben 0,04 ml/kg bobot badan) sedangkan kelompok P0 bertindak sebagai kontrol tanpa pengobatan. Hasil penelitian ini menunjukkan bahwa pengobatan dengan herbal serbuk biji pepaya matang pada babi ascariasis ternyata mampu menurunkan daya berembrio telur cacing dalam tinja babi dan dalam uterus cacing secara efektif sehingga dapat digunakan untuk pengendalian askariasis pada babi.
Co-Authors Adryani Ris Akira Ito Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Anak Agung Sagung Kendran BERNA NATALIA SILABAN Desak Nyoman Dewi Indira Laksmi Dewa Putu Sahadewa Dharmawan N.S Dina Harumna Endang Wulandari Suryaningtyas Farika E. Y Gede Yudi Suryawan Handayani, Ni Made HERTATI ANRIANI LUBIS I Gede Mahardika I K. Berata I K. K. Agustina I Kadek Saka Wiryana I Kadek Swastika I Ketut Anom Dada I Ketut Berata I Ketut Suada I Made Angga Prayoga I Made Bakta I Made Dwinata I MADE INDRA PERMADI I Made Kardena I Made Merdana I Made Subrata I Nengah Kapti I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Sunita I Nyoman Wijaya Guna I PUTU YOGA LOKANTARA I Wayan Bebas I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan I. B. Putra-Manuaba Ida Ayu Laksmi Puspita Dewi Ida Ayu Pasti Apsari Ida Bagus Gde Yudha Triguna Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya K. K. Agustina Kadek Agus Agra Arnawa Kadek Ayu Dwi Suryastini Kadek Dianiyanti Kadek Karang Agustina Ketut Budiasa Luh Dewi Anggreni MARIANA KRESTY FERDINANDEZ MORI FRISKA TAMBA Muhammad Ulqiya Syukron Munehiro Okamoto N. T. Suryadhi N.S. Dharmawan Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Luh Ayu Trisnayanti Ni Luh Gede Merry Cintya Laksmi Ni Luh Gede Sudaryati Ni Made Yunik Novita Dewi Dewi Nyoman Tigeh Suryadi PANDE KETUT YULIARI PRATIWI DEVI GM Putu Nara Kusuma Prasanjaya Putu Suma Githa Sanjaya Putu Sutisna sang gede purnama Sri Hartini STEFAN THEISEN Stelen, I Dewa Ketut Raeyadi Purnama Sudiartawan, I Putu Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun, Tjok Gede Oka TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia