Article Per Year (5 Year)
p-Index From 2021 - 2026
1.554
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu dan Teknologi Hasil Hutan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Forum Pasca Sarjana Jurnal Sain Veteriner Biospecies Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia Prosiding Seminar Biologi Microbiology Indonesia AL KAUNIYAH Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Matematika & Sains Jurnal Ilmu Kelautan Spermonde agriTECH TREUBIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Floribunda Jurnal Ilmu Kefarmasian Indonesia Konservasi Hayati Zoo Indonesia Proceeding Biology Education Conference Makara Journal of Science Jurnal Kedokteran Hewan Jurnal Kefarmasian Indonesia
TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary Other

Published : 87 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Microbiology Indonesia

 1 
Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.493 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.
Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.828 KB) | DOI: 10.5454/mi.7.2.3

Abstract

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.
Co-Authors Abdul Rahman Singkam Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Suryani Antonius Suwanto Any Aryani Ardi Kapahang Arief Boediono Arlyza1, Irma Shita Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Daniel Happy Putra Dedi . Soedharma Dedi Soedharma DEDI SOEDHARMA DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia DIAH ISKANDRIATI Diah Iskandriati DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa Evy Ayu Arida EVY AYU ARIDA Fahma Wijayanti Fahri Fahrudin, Fahri Faisal Mustafa Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra Haerul, Andi HAJRIAL ASWIDINNOOR Handayani , Handayani Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Indah Fajarwati, Indah Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jakaria Jakaria Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo LILIK BUDIPRASETYO Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior Mahmud, Rois MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Alias Butet Pamungkas, Joko Prasetyo Prasetyo Priyono, Siti N. Puji Rianti Retno Damayanti Soejoedono RICHARD F GRANT RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito SUHARSONO Suharsono Suharsono SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani, Sulistiyani Surjono Surjokusumo Syafyudin Yusuf Syaiful Anwar Syamsul Bachry Taher, Achmad Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri Tutik Wresdiyati UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yus Rusila Noor Yusnarti Yus