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Fevria, Resti
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Journal : Tropical Genetics

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Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.
Co-Authors -, Vauzia - -, Yulkifli - A. Ardi Abdul Razak Abdul Razak Achyar, Afifatul Afiah, Annisa Afrilisia, Lora Agustian Agustian Aini, Quratul Alqaramah, Rachel Amalia Fildzah Sharfina Andi Alatas, Andi Annisa Annisa Tulhasanah Annisa, Silvy Ardi Ardi Ardi Ardi Arjulis, Wanda Atifah, Yusni Audela Oktavira Azwir Anhar cynthia perdana putri Delvia Suarman Des M Des M Des M. M. Dezi Handayani Dhea Sabrina Aprilia Dona, Afrini Dwi Hilda Putri Edwin Edwin Edwin Edwin Edwin Edwin Elisia, Rini Eliza Octa Khairani Elsa Badriyya Eni Kamal Erwina Simanullang Fadilla Meidita Fadilla Sonia Putri Fajri Rifyant Febriani, Zulfa Fitria Anwar Ghiffari, Muhammad Helendra . Hermansah Hermansah, Hermansah Humulusna, Rira Indra Hartanto Indra Hartanto Irdawati Irdawati Irma Lailani Putri Irma Leilani Eka Putri Isril Berd Kardiman, Reki Kiki Amelia, Kiki Kudususalam, Malikil Linda Advinda M Maizirwan Maiyontoni, Maiyontoni Mardaniyah, Annisa Marni, Yunis Mitason, Deka Monica , Indiastri P Moralita Chatri nada wafiq hijriah, Nada wafiq Nia Faradila Niken Maharani Putri Novelina, Agnes Ramelin Novita Sukawati Nurmiati Nurmiati Nurul Fadilah Oktaviani, Mutia Olan Tri Sinaga Pertiwi, Lastri Purnamasari, Devi Putra, Ahadul Putri Oktavia Putri, Afriani Amelia Putri, Amelia Dwi Putri, Fanesha Panca Putri, Gusti Dinia Putri, Irma Leilani Eka Putri, Santi Diana Qurrata A'yuni A'yuni Rafiqi, Aufa Rahmadhani Fitri Rahmi, Elva Ramadhan Sumarmin Rara Via Jonet REFIKA KOMALA Reki Kardiman Rijal Satria Rika Andima Ririn Indria Sari Ristiono Ristiono Rizka Meisy Evis Putri Rizki Rahman Putra Roisiah, Qoimatun Roni Jarlis S. Syamsurizal S. Syamsurizal Sania, Hani Selaras, Ganda Hijrah Sintia Delia Syafitri Siska Farma Sri Mutiar, Sri Supriyanti, Juli Syamsurizal Syamsurizal Syuhada, Fildza Arief Taufiqqurrahman, Taufiqqurrahman Tazri, Mufidah Insani Vauzia Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Vivi Hendrita Wahyudi Rahmat Wilna Sari Wita Febriani Yovella Trisna Wijaya Yulkifli Yulkifli Yurico Utami