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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Edukasi Universitas Jember Journal of Education and Learning (EduLearn) Biogenesis: Jurnal Ilmiah Biologi Jurnal Pendidikan Biologi Indonesia JPS Unimus Jurnal Florea Phenomenon : Jurnal Pendidikan MIPA Bio Ilmi: Jurnal Pendidikan JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Farmasi Sains dan Komunitas Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika BIOEDUKASI Journal of Science Learning Biosfer: Jurnal Pendidikan Biologi LENSA (Lentera Sains): Jurnal Pendidikan IPA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Cetta: Jurnal Ilmu Pendidikan Jambura Edu Biosfer Journal Jurnal Mangifera Edu Majalah Biomorfologi (Biomorphology Journal) Academia Open Integrated Science Education Journal Tafkir: Interdisciplinary Journal of Islamic Education Proceeding Biology Education Conference Pancaran Pendidikan Jurnal Pendidikan MIPA Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Indonesian Journal of Science and Education
Erlia Narulita
Universitas Jember (SCOPUS ID: 56521650500)
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Immunomodulatory Activity of Ganyong Tuber (Canna edulis K.) Extract on Phagocytosis, Leukocytes, and Antibody of Mice against Bacterial Infections Savira, Nadyatul Ilma Indah; Qulbi, Siti Shofa Assyifa; Marditianingsih, Meilia Putri; laili, Mumtahana; Narulita, Erlia; Pramudya, Manikya; Maulana, Alfan Irsyad
Majalah Biomorfologi Vol. 35 No. 2 (2025): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i2.2025.113-122

Abstract

Highlights Ganyong tuber exhibits immunomodulatory activity against Staphylococcus aureus infection in mice. Ganyong tuber extract increases phagocytic activity, leukocyte count, and spleen weight in infected mice.   ABSTRACT Background:  Ganyong tubers (Canna edulis K.) are widely consumed by Indonesians as an alternative food source. However, no studies have investigated the pharmacological properties of ganyong tubers, particularly their immunomodulatory activity. Objective: This study aimed to evaluate the immunomodulatory effects of ganyong tubers in mice infected with Staphylococcus aureus. Material and Method: This study used a randomized experimental design involving 30 male BALB/c mice divided into five groups, including a normal control, a negative control (infected with Staphylococcus aureus), and three groups treated with ganyong (Canna edulis K.) extract at doses of 50, 100, and 150 mg/kg BW. The extract was administered orally for seven days, followed by S. aureus injection. Phagocytic activity, leukocyte count, spleen weight, and antibody levels were evaluated using microscopy and ELISA. Data were analyzed using one-way ANOVA and Duncan’s post hoc test. Result: This study evaluated the immunomodulatory effects of ganyong tuber (Canna edulis K.) extract on phagocytosis activity and capacity, leukocyte count, spleen weight, and antibody levels in mice infected with Staphylococcus aureus. Phagocytosis activity and capacity were highest in the group receiving 50 mg/kg body weight (BW) extract, with decreasing effects at higher doses. Leukocyte counts were highest in the infected untreated group, while spleen weight was significantly increased in the 50 mg/kg BW treatment group compared to controls. Although the 100 mg/kg BW group showed the highest antibody levels, these differences were not statistically significant. Conclusion: Ganyong tuber extract demonstrated immunomodulatory activity by enhancing innate immune responses against S. aureus infection.
The Effect of Socio-Scientific Issue (SSI) Science Learning Based on The Decision Making Skills of Eight Grade Junior High School Student: PENGARUH PEMBELAJARAN IPA BERBASIS SOCIO-SCIENTIFIC ISSUE (SSI) TERHADAP KETERAMPILAN PENGAMBILAN KEPUTUSAN SISWA SMP KELAS VIII Narulita, Erlia
Indonesian Journal of Science and Education Vol. 6 No. 2 (2022): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

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Abstract

Decision-making skills are an activity to make the best decision from several alternative solutions offered with certain considerations. This study aims to examine the effect of Socio-Scientific Issue (SSI)-based science learning on the decision-making skilss of class VIII Junior High School students. The research method used is a quasy experiment with a non-equivalent control group design. The population in this study was class VIII SMP Negeri 7 Jember in the academic year 2021/2022. The selection of research samples using purposive sampling technique obtained class VIII B as the control class and class VIII E as the experimental class. The results of the analysis used the Independent Sample T-test with a significance value og 0.041 < 0.05. In addition, based on the N-Gain test in the control class the average N-Gain is 0.4233 with moderate criteria in the less effective category, while the average N-Gain in the experimental class is 0.5568 with moderate criteria being quite effective. Based on the results of the study, it can be concluded that there is a significant effect on Socio-Scientific Issue (SSI)-based science learning on the decision-making skills of class VIII Junior High School students. Analysis with the N-Gain test shows that SSI-based science learning is included in the category of being quite effective in learning with a precentation of 56 percent. Keywords: Science Learning, Socio-Scientific Issue, Decision-making Skills
Validated Culturally Responsive Science Assessment Using Integrated Content and Construct Analysis Ali, Aisyah; Bektiarso, Singgih; Walukow, Auldry Fransje; Narulita, Erlia; Kadir, Akhmad
Academia Open Vol. 10 No. 2 (2025): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.10.2025.12776

Abstract

(General Background) The alignment of science assessment with students’ socio-cultural contexts is essential to ensure fairness and meaningful measurement of learning outcomes. (Specific Background) However, most contextualized assessments in science education emphasize content validity without empirically confirming their construct structure, limiting their interpretive strength. (Knowledge Gap) There remains a lack of studies that integrate content, empirical, and construct validity evidence in culturally responsive instruments, particularly those designed in parallel pre–post forms. (Aims) This study aimed to develop and validate an ethnoscience-based pre–post instrument by linking Aiken’s Content Validity Ratio (CVR, 4-point scale) with Confirmatory Factor Analysis (CFA, CR/AVE). (Results) Findings from five expert reviews showed 22 of 40 items exceeded the conservative threshold (Aiken’s V ≥ 0.80; CVR = 1.00). Field trials (N = 50) demonstrated moderate difficulty and positive discrimination, while CFA confirmed a three-factor structure with good fit (χ² = 34.203, df = 24, p = 0.083; CFI = 0.94; TLI = 0.92; RMSEA = 0.065). Composite reliability ranged from 0.718–0.797, and AVE was adequate for two factors (0.506; 0.568) and marginal for one (0.459). (Novelty) The study presents a transparent “content–empirical–construct” decision trail rarely reported in ethnoscience assessment. (Implications) This integrative validation framework demonstrates that cultural responsiveness and psychometric rigor can coexist, guiding fair and contextual science learning evaluations. Highlights: Integrates Aiken–CVR and CFA for comprehensive validity evidence. Confirms three-factor model with strong reliability and moderate AVE. Demonstrates synergy between cultural relevance and measurement rigor. Keywords: Content Validity, Confirmatory Factor Analysis, Ethnoscience, Culturally Responsive Assessment, Psychometric Validation
Bacteriophage-Based Wound Care Formulation for Diabetic Ulcers as A Growth Inhibitor of Alcaligenes faecalis Kiswara, Nur Hanifah Alim; Ludfillah, Rayyan Zhafir; Febrianti, Riska Ayu; Narulita, Erlia; Winarti, Lina
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.44082

Abstract

Diabetic ulcers are frequently complicated by infections from multidrug-resistant bacteria such as Alcaligenes faecalis, necessitating the development of alternative therapeutic strategies. Bacteriophage therapy presents a promising approach due to its specificity and efficacy against antibiotic-resistant pathogens. This study aimed to formulate and evaluate a bacteriophage-based topical cleanser using the phage ϕAFaV1 for treating A. faecalis infections, focusing on high antibacterial efficacy and low dermal toxicity. Three suspension formulas (F1, F2, F3) were prepared with varying chitosan concentrations (0.5; 0.75, and 1.0%) as a suspending agent. These formulations underwent physical stability testing over 28 days, including assessments of viscosity, pH, zeta potential, and homogeneity. Antibacterial efficacy was determined through inhibition zone assays. All three formulations effectively inhibited bacterial growth, forming clear zones. The F2 formula (0.75% chitosan), containing 200 µL of active phage ingredient, was identified as the optimum formulation. It demonstrated superior physical stability and the most effective bactericidal activity against A. faecalis, as confirmed by statistical analysis. These results indicate that a bacteriophage suspension stabilized with 0.75% chitosan is a highly promising and stable topical agent for combating multidrug-resistant A. faecalis in diabetic wound care.
The Effects of Team Games and Tournament Integrated with STEM 3D Audio-Visual Kit for Enhancing Collaboration Skills and Learning Outcomes Billa, Attika Salsa; Narulita, Erlia; Amalia, Rachma Fadhillah; Supeno, Supeno
Jurnal Pendidikan MIPA Vol 25, No 3 (2024): Jurnal Pendidikan MIPA
Publisher : FKIP Universitas Lampung

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Abstract

Collaboration is one of the essential skills that students must have in the global era. With collaboration skills, students can interact in groups and take responsibility for solving problems, impacting learning outcomes. However, integrating collaboration skills in learning still challenges teachers and students. Therefore, it is necessary to pilot innovative learning media to promote collaboration skills in learning activities. This study examined the impact of using STEM-based 3D audio-visual protein synthesis kit box learning media on collaboration skills and learning outcomes. The study was conducted on SMA Negeri 1 Tanggul students, Jember Regency, East Java. The study used a pre-test and post-test control group design. The experimental class was treated using the TGT learning model with STEM learning media, while the control class applied traditional learning. This study used two types of groups, namely large groups and small groups, which aimed to determine the effect of the number of members in the group on collaboration skills. Based on the one-way ANOVA test analysis in the control and experimental classes, the research results obtained a sig < 0.05 with the hypothesis Ha rejected, which means there is a difference in collaboration skills between students in large and small groups. The one-way ANOVA test analysis results in the control and experimental classes obtained a sig < 0.05 with the hypothesis H0 rejected, which means there is a difference in student learning outcomes.        Keywords: collaboration skills, learning outcomes, team games and tournaments (TGT).DOI: http://dx.doi.org/10.23960/jpmipa/v25i3.pp1151-1164
Nutritional Investigation, LC-MS-Based Phytochemical Profiling, and Antioxidant Assay of Two Edible Flowers Kuswati, Kuswati; Zahro, Aliza; Daud, Nadhiratuzzahra Alifiah; Narulita, Erlia; Fatmawati, Yayuk
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.404-415

Abstract

Two commonly consumed, yet under-researched, edible flowers—Malvaviscus arboreus (Topi Turki) and Acmella paniculata (Jotang)—were comprehensively analyzed to assess their potential as functional food sources. This study investigated their nutritional content, phytochemical profiles, antioxidant activity, and organoleptic properties. Our methodology, conducted between July and December, 2024, involved a multi-faceted approach—proximate analysis quantified ash, moisture, protein, fat, fiber, carbohydrates, and total energy. Freeze-dried samples underwent LC-MS for phytochemical identification, and antioxidant activity was determined using the DPPH assay. Organoleptic preferences were evaluated through a hedonic test where 30 panelists rated color, aroma, taste, and overall acceptance. Key findings revealed distinct differences. A. paniculata presented higher protein (18.75%), fat (21.71%), and fiber (24.10%), leading to a greater total energy (213.84 kcal/50g). In contrast, M. arboreus showed higher moisture (21.22%) and carbohydrates (48.12%). Phytochemical profiling by LC-MS indicated that M. arboreus contained 51 phytochemicals, primarily phenolics (13.52%), while A. paniculata had a remarkable 170 phytochemicals, dominated by alkaloids (2.94%). Importantly, M. arboreus demonstrated superior antioxidant activity (IC50 92.74 µg/mL, strong) compared to A. paniculata (IC50 156.95 µg/mL, weak) in the DPPH assay. Organoleptically, M. arboreus was preferred for its color, taste, and overall acceptability, with no significant difference in aroma. Overall, both M. arboreus and A. paniculata exhibit promising nutritional value and bioactive potential for functional food applications. This research highlights the significant potential of these edible flowers to diversify plant-based diets and contribute to the development of novel health-promoting products. Future research should focus on optimizing processing techniques and exploring diverse food applications to maximize their utilization.
Phage cocktails as a new antibiotic for combating bacteria consortium in foodborne disease Marzuqi, Salsabila Jahroh; Putri, Adelia Meita; Purwanto, Martinus Effand Pratama; Murtadho, Ahmad Hibban; Grahita, Amanda Claudia Ayuning; Narulita, Erlia
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2394

Abstract

Foodborne diseases pose a critical global health threat, exacerbated by the alarming rise of multidrug-resistant (MDR) bacterial strains like Staphylococcus aureus, Escherichia coli, and Salmonella typhi. Conventional antibiotics are increasingly ineffective, necessitating the development of alternative therapeutic and biocontrol strategies. This study aims to evaluate the efficacy and stability of a bacteriophage cocktail as a potential non-antibiotic alternative for controlling a consortium of foodborne pathogenic bacteria. The research employed an experimental design using revived cultures of the three bacterial species and three phage isolates (ϕSZUT-01, ϕSZIP-01, ϕSZIP-02) as research samples. Data collected included bacterial growth kinetics, observation of lysis zones, and phage stability at various pH levels and storage temperatures. Data analysis was conducted descriptively, followed by one-way ANOVA at a 95% confidence level to determine differences among treatments. Three two-phage cocktails were formulated and tested, with all treatments consistently demonstrating strong lytic activity, evidenced by large and clear plaques. Lysis kinetics, monitored through OD600 measurements, confirmed rapid bacterial elimination beginning approximately 180 minutes post-infection. Notably, the bacterial consortium exhibited resistance to the positive control (Chloramphenicol), reinforcing the need for alternative interventions. Additionally, the phage cocktail demonstrated excellent stability, maintaining infectious titers across a broad pH range and during storage at both refrigerated (4°C) and room temperatures (21°C). This high stability supports its potential applicability in food safety systems and resilience in gastrointestinal environments. Overall, the bacteriophage cocktail demonstrated effective antibacterial activity against a consortium of foodborne pathogenic bacteria, as evidenced by the formation of clear lysis zones and exhibited high stability, supporting its potential application as a non-antibiotic alternative for controlling foodborne diseases.
Analisis Kebutuhan Pengembangan Modul IPA Berbasis Etnosains Sumenep untuk Meningkatkan Literasi Sains dan Karakter Gotong Royong Peserta Didik Farida, Nur; Narulita, Erlia; Wicaksono, Iwan
Cetta: Jurnal Ilmu Pendidikan Vol 9 No 1 (2026)
Publisher : Jayapangus Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37329/cetta.v9i1.5109

Abstract

Science learning at the junior secondary school level is expected to foster students’ scientific literacy and collaborative character in line with the Merdeka Curriculum. However, classroom practices still reveal a gap between these objectives and actual learning conditions. This article reports the needs analysis stage of a Research and Development (R&D) study employing the ADDIE model. The study aims to analyze the need for developing an ethnoscience-based science module grounded in the local context of Sumenep to support students’ scientific literacy and collaborative character. The needs analysis was conducted through a review of the junior secondary school science curriculum, semi-structured interviews with five science teachers, and questionnaires administered to 561 students from grades VII, VIII, and IX at SMP Negeri 1 Sumenep. The research instruments consisted of needs assessment questionnaires comprising nine items for students and eleven items for teachers. The questionnaires were developed based on scientific literacy indicators and collaborative character, validated through expert judgment, and demonstrated adequate reliability. Data were analyzed descriptively using mean scores and percentages. The results indicate that students experience difficulties in understanding science concepts, explaining scientific phenomena, and interpreting data and scientific evidence. In addition, science instruction has not been optimally integrated with local cultural contexts. Both teachers and students consistently expressed a strong need for contextual, collaborative, and ethnoscience-based science learning grounded in Sumenep local culture. The study concludes that developing an ethnoscience-based science module integrated with scientific literacy and collaborative character is necessary as a foundation for subsequent design and development stages.
Development and Validation of the Affiliation–Interpersonal Skills Questionnaire (AIS-Q) for Culturally Responsive Science Learning in Pre-Service Elementary Teachers Aisyah Ali; Singgih Bektiarso; Auldry Fransje Walukow; Erlia Narulita; Akhmad Kadir
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13064

Abstract

This study addresses the need for culturally responsive science learning in elementary teacher education by developing and empirically examining Affiliation–Interpersonal Skills (AKI), comprising affiliation motivation and interpersonal skills, within an ethnoscience learning context. Using a cross-sectional design, the study involved 50 prospective elementary teachers from a public university in Papua, Indonesia, most of whom were from disadvantaged, frontier, and outermost areas. Content validity was evaluated by five experts using the Content Validity Index, while item performance and internal consistency were examined through descriptive statistics, corrected item–total correlations, and reliability analysis. The results showed that the AKI instrument demonstrated strong content validity (most items I-CVI ≥ 0.78; S-CVI/Ave ≥ 0.90) and satisfactory reliability (subscale and total coefficients ≥ 0.70). Empirically, participants exhibited generally moderate to high affiliation motivation, reflecting a strong tendency toward collaboration and social connectedness, while interpersonal skills varied across individuals, particularly in empathic communication and conflict management. Based on these patterns, three AKI profiles were identified, indicating differentiated training needs in ethnoscience-based science learning. These findings suggest that AKI is a measurable and meaningful construct for mapping prospective teachers’ social–interpersonal readiness and for informing targeted interventions to strengthen culturally responsive science instruction.
Development of Super Deformed Digital Manga Comic Media Using Microsoft Paint to Improve Learning Outcomes and Learning Motivation of Junior High School Students on the Earth and Solar System Material Alfarisi, Muhammad Fahmi; Mahardika, I Ketut; Narulita, Erlia
Integrated Science Education Journal Vol 7 No 2 (2026): March
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/isej.v7i2.2904

Abstract

Purpose of the study: Learning media used in teaching must increase student interest and motivation. An example is comics. This research on the development of digital super deformed manga comics has several objectives: to assess the media's validity, to test its practicality, and to test its effectiveness. Methodology: The research used is a type of Research and Development (R&D) research that provides validation of the developed product to be used in the learning process. The development model used in this study is the 4D model, which consists of 4 stages: defining (define), planning (design), developing (development), and disseminating (disseminate). Main Findings: The study achieved a validity level of 91.5% and was deemed valid. The results of the practicality of comic media in increasing learning motivation were 91.875% in the first school, 90.625% in the second school, and 92.5% in the third school. Meanwhile, the N-Gain scores indicating an increase in learning outcomes were 0.81 in the first school, 0.63 in the second school, and 0.69 in the third school. Novelty/Originality of this study: A super deformed digital manga comic was created using Microsoft Paint. This super deformed digital manga comic, in addition to using a chibi or super-deformed style, also imitates the drawing style of Masashi Kishimoto, the creator and writer of the Naruto series. This novel comic medium has successfully improved student learning outcomes and motivation.
Co-Authors .,, Rasmiyana Agatha, Nabiela Dini Agviolita, Putri Aini Meitanti Rosalina Aisyah Ali Akhmad Kadir Al Hadi, Salma Khatami Alfarisi, Muhammad Fahmi Ali, Aisyah Amala Sholeha Apridza Amalia, Rachma Fadhillah Anjar Putro Utomo Anusorn Kongsawang Areerat Chuseng Aris Singgih Budiarso Auldry Fransje Walukow Bambang Sugiharto Bella, Frimita Bevo Wahono Billa, Attika Salsa Daud, Nadhiratuzzahra Alifiah Dewi, Ratna Sari Dyah Intan Prismasari Faizah Firdaus Fatmawati Ningsih Febrianti, Riska Ayu Fikri Ainur Risma Hardiyanti Oktavia Gerda Permata Aji Grahita, Amanda Claudia Ayuning HARDIAN SUSILO ADDY Hilya Wildana Sofia Holida Isnania I Ketut Mahardika Iir Nur Choiriya Ika Lia Novenda Ima Masafatus Sholihah Iryani Endah Febrianti Jekti Prihatin Kanoklada . Khairunnisa, Icha Khoirul Anam Kiswara, Nur Hanifah Alim Krisianti Ayu Monita Kuswati Kuswati laili, Mumtahana Lina Winarti Linda Kusumawati Ludfillah, Rayyan Zhafir Lum’atul Khoirot Rizki Luthfia, Syarifattul M. Amien Rais Manikya Pramudya Marditianingsih, Meilia Putri Marzuqi, Salsabila Jahroh Maulana, Alfan Irsyad muhammad rizqi nur Murtadho, Ahmad Hibban Nabiela Dini Agatha Nadyatul Ilma Indah Savira Neliana, Intan Ria Nur Ahmad Nur Farida Nurul Aslami Nury Qurrota Ayun Pramudya Dwi Aristya Putra Purwanto, Martinus Effand Pratama Putri, Adelia Meita Qulbi, Siti Shofa Assyifa Ratna Sari Dewi Ria Yulian Rif’atul Fitri Supa’at Rohmah Jwita Sari Rusdianto Rusmala Evi Anggraeni S Suratno Singgih Bektiarso Siti Murdiyah Slamet Hariyadi Slamet Hariyadi Slamet Hariyadi Sofia, Hilya Wildana Sri Wahyuni Sri Wahyuni Sudarti Sudarti Supeno Suratno Suratno Sylvia Rimbanita Purwanto Ulin Nuha Vendi Eko Susilo Wachju Subchan Walukow, Auldry Fransje Wicaksono, Iwan Widhi Dyah Sawitri Yayuk Fatmawati Yayuk Fatmawati, Yayuk Yeni Firdayanti Yulian, Ria Zahro, Aliza