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All Journal JBIO: jurnal biosains (the journal of biosciences) Journal of Environmental Engineering and Sustainable Technology Edumatsains BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA JURNAL PENDIDIKAN TAMBUSAI Jurnal Pembelajaran dan Biologi Nukleus Jurnal Biogenerasi Jurnal Agro Indragiri Jurnal Pengabdian kepada Masyarakat Jurnal sains dan teknologi industri peternakan Journal of Natural Sciences Jurnal Abdi Masyarakat Indonesia Mejuajua JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM) QISTINA: Jurnal Multidisiplin Indonesia Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Bakti Sekawan : Jurnal Pengabdian Masyarakat Jurnal Pengabdian Masyarakat dan Riset Pendidikan Jurnal Mahasiswa Kesehatan Jurnal Ekonomi Pertanian dan Agribisnis
Situmorang, Nurbaity
Prodi Ilmu Gizi Universitas Nahdlatul Ulama Sumatera Utara
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Aktivitas Antibakteri Ekstrak Daun Jeruk Purut (Citrus Hystrix DC) Terhadap Bakteri Uji Pada Bakso Bakar: Penelitian Ginting, Silvia Margaretta br; Purba, Melpa Yusmawati; Tamba, Gelora Betania; Pasaribu, Maria Benedikta; Saragih, Elsa Angelina; Gultom, Endang Sulistyarini; Situmorang, Nurbaity
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 2 (October 202
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i2.3526

Abstract

Indonesia, as a tropical country, is rich in essential oil-producing plants, including kaffir lime (Citrus hystrix DC). The leaves of Citrus hystrix are known to contain bioactive compounds such as flavonoids, tannins, and alkaloids, which have potential as natural antibacterial agents. This study aimed to evaluate the antibacterial activity of Citrus hystrix leaf extract against bacteria present in grilled meatballs. An in vitro experimental method using the disc diffusion assay was employed. Fresh Citrus hystrix leaves were obtained through purposive sampling from Medan Krio Village, North Sumatra, extracted using 96% ethanol, and tested against bacteria on Mueller Hinton Agar. Chloramphenicol was used as a positive control, while dimethyl sulfoxide (DMSO) served as a negative control. The results showed inhibition zones of 8.02 mm and 7.09 mm for the leaf extract in two separate trials, whereas the positive control exhibited a 29.91 mm inhibition zone, and no inhibition was observed in the negative control. These findings indicate that Citrus hystrix leaf extract possesses significant antibacterial activity against bacteria in grilled meatballs, although it is less potent than synthetic antibiotics. The extract has potential as a natural antibacterial agent for food preservation.
Ekstraksi Andaliman (Zanthoxylum acanthopodium DC,) Sebagai Obat Kumur Herbal (Herbal Mouthwash) Mustasfa, Sinta Wulida; Dalimunthe, Aina Mardiah; Mutia, Diska Qolbu; Elisabet, Melfa Romanna; Saragih, Putri Fransiska; Gultom, Endang Sulistyarini; Situmorang, Nurbaity
QISTINA: Jurnal Multidisiplin Indonesia Vol 4, No 2 (2025): December 2025
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/qistina.v4i2.7325

Abstract

Tanaman andaliman (Zanthoxylum acanthopodium DC.) merupakan rempah asli Sumatera Utara yang kaya akan senyawa aktif seperti flavonoid, alkaloid, tanin, dan saponin, yang berfungsi sebagai antibakteri alami. Studi ini dilakukan untuk mengevaluasi potensi ekstrak andaliman dalam pembuatan obat kumur berbasis herbal. Ekstraksi bahan dilakukan menggunakan metode maserasi dengan pelarut etanol 70%, kemudian diuji aktivitas antibakterinya terhadap mikroorganisme penyebab bau mulut seperti Streptococcus mutans dan Staphylococcus aureus. Hasil menunjukkan bahwa ekstrak andaliman mampu menghambat pertumbuhan bakteri secara efektif dengan terbentuknya zona hambat yang jelas. Formulasi obat kumur dari ekstrak andaliman juga menunjukkan keamanan penggunaan serta memberikan efek penyegaran alami di rongga mulut. Oleh karena itu, andaliman dapat dijadikan sebagai alternatif bahan alami yang potensial untuk dikembangkan menjadi obat kumur herbal yang efektif dan ramah lingkungan.
Aktivitas Antimikroba Ekstrak Etanol Daun Kenanga (Cananga Odorata) Terhadap Mikroorganisme Terhadap Isolat Bakteri Tanah Damaiyani, Damaiyani; Salsabilah, Talita; Nura S, Alfi; Denisa, Rifka; Fauziah, Ennita; Gultom, Endang Sulistyarini; Situmorang, Nurbaity
QISTINA: Jurnal Multidisiplin Indonesia Vol 4, No 2 (2025): December 2025
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/qistina.v4i2.7324

Abstract

Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak etanol daun kenanga (Cananga odorata) terhadap bakteri isolat tanah. Penelitian dilakukan secara eksperimental laboratorium (in vitro) dengan menggunakan metode difusi cakram pada media Mueller-Hinton Agar (MHA). Daun kenanga diekstraksi menggunakan etanol 96% melalui metode maserasi dan remaserasi hingga diperoleh ekstrak kental, kemudian diuji pada konsentrasi 100% dan diamati secara kualitatif berdasarkan terbentuknya zona hambat setelah inkubasi selama 24 jam pada suhu 37°C. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kenanga memiliki aktivitas antibakteri dengan zona hambat sebesar 8,5 mm dan 8,16 mm yang tergolong kategori sedang. Aktivitas antibakteri ini disebabkan oleh kandungan metabolit sekunder seperti flavonoid, tanin, saponin, alkaloid, dan minyak atsiri. Meskipun daya hambatnya lebih rendah dibandingkan antibiotik kloramfenikol (22–24 mm), hasil ini menunjukkan bahwa daun kenanga berpotensi sebagai sumber antibakteri alami yang efektif dan ramah lingkungan.
Aktivitas Antibakteri Ekstrak Bunga Kecombrang (Etlingera elatior) Terhadap Bakteri Uji pada Cangkang Telur Pandiangan, Putri Patricia; Lumbantoruan, Lamtiurma; Ndaha, Gebyta Yani; Ester, Ester; Kaban, Hairen Br; Gultom, Endang Sulistyarini; Situmorang, Nurbaity
AURELIA: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Vol 5, No 1 (2026): January 2026
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/aurelia.v5i1.7330

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol bunga kecombrang (Etlingera elatior) terhadap bakteri yang diisolasi dari permukaan cangkang telur. Kontaminasi bakteri, khususnya Salmonella sp., sering ditemukan pada cangkang telur dan berpotensi menyebabkan penyakit bawaan pangan (foodborne disease). Bunga kecombrang diketahui mengandung senyawa bioaktif seperti flavonoid, alkaloid, tanin, saponin, dan minyak atsiri yang berpotensi sebagai antibakteri alami. Penelitian ini menggunakan metode eksperimental laboratorik in vitro dengan rancangan post-test only control group design. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram dengan tiga perlakuan: ekstrak etanol bunga kecombrang (hasil evaporasi), kontrol positif (kloramfenikol), dan kontrol negatif (DMSO). Hasil pengamatan menunjukkan bahwa ekstrak etanol bunga kecombrang menghasilkan zona hambat 4,18 mm (vertikal) dan 4,49 mm (horizontal) yang tergolong lemah, sedangkan kontrol positif menghasilkan zona hambat 27,44 mm (sangat kuat) dan kontrol negatif tidak menunjukkan hambatan. Hasil ini membuktikan bahwa ekstrak bunga kecombrang memiliki aktivitas antibakteri terhadap bakteri cangkang telur, meskipun efektivitasnya masih lebih rendah dibandingkan antibiotik standar. Senyawa flavonoid, tanin, dan minyak atsiri diduga berperan dalam aktivitas antibakteri melalui mekanisme perusakan membran sel dan penghambatan sintesis protein. Dengan demikian, bunga kecombrang berpotensi dikembangkan sebagai antibakteri alami pengganti antibiotik sintetis.
PENANAMAN POHON MATOA DAN SEMPUR DI BANTARAN PINGGIRAN SUNGAI DESA SUKA MAKMUR KECAMATAN SIBOLANGIT Ramadhan, Nuri; Hendra, Hendra; Hariati, Emy; Malinda, Leni; Situmorang, Nurbaity; Hidayat, Wahyu; Novina Sukapiring, Dewi; Putri, Nizmi; Febriantika, Febriantika; Virginia Tamara , Chatrine
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 4 No 2 (2023): Jurnal Pengabdian Pendidikan Masyarakat Vol 4 No 2 (2023)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v4i2.1264

Abstract

Community service activities for lecturers are an effort to carry out one of the tri dharma tasks of higher education. This service activity aims to educate the public about the importance of protecting the environment around river basins by planting productive trees. In addition, the community can also understand the importance of reforestation to restore natural conditions, especially river banks which will be damaged due to natural and human factors. The implementation of this community service activity was carried out by providing an open green space for the elderly and the local community on May 28 2022 in the riverbank area of ​​Jl. Letjen Jamin Ginting Km 45 Gelora Kasih GBKP 100 Year Jubilee Park Complex, Suka Makmur Village, Sibolangit District, Deli Serdang Regency, North Sumatra Province. This Community Service is very beneficial for the elderly who are in the area where the tree is planted. This greening culture must be familiarized from an early age through the practice of planting trees. By frequently planting trees, it is hoped that it can increase people's awareness and love for preserving and protecting the environment, especially watersheds. With this Community Service activity, it is hoped that it will help the community to realize the importance of protecting the environment. The expected output targets from this activity are, First, provide an open green space for the elderly and the local community. Second, growing the local community's economy from the production of matoa and simpur tree seeds. Third, Improving the welfare of the surrounding community and the elderly.
GROWTH RESPONSE OF GREEN BEANS (Phaseolus radiatus) TO WATERING INTERVALS Panggabean, Nurul Huda; Yudha Pratama, Rizky; Rahmadina, Rahmadina; Faisal Nasution, Ahmad; Situmorang, Nurbaity; Kairani, Anita
JURNAL BIOSAINS Vol. 12 No. 1 (2026): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/cfzm0080

Abstract

Green beans (Phaseolus radiatus) are one of the commodity of legume plants that are generally grown on dry land, Green beans are included in plants that are tolerant of water shortages, the important thing is that the soil has enough moisture. The problem that is often faced in mung bean cultivation in dry land is the low production achieved by farmers, so it is necessary to conduct research on the resistance of mung beans (Phaseolus radiatus) to drought and the effect of watering frequency on productivity. The purpose was to determine the watering interval to the growth response of mung bean plants (Phaseolus radiatus). This study used an experimental design using a Group Random Design (RAK) consisting of 4 treatments with 3 replicas of each treatment. F0U1, F0U2, F0U3 : Control Treatment (No Watering), F1U1, F1U2, F1U3 : Watering frequency 1 (one) time a day. F2U1,F2U2, F2U3 : Watering frequency 2 (two) times a day. F3U1, F3U2, F3U3 : Watering frequency 3 (three) times a day. The parameters in calculating the growth response are the length of the plant, the number of leaves and the height of the plant. The best watering interval for plant length is watering once every 1 (one) day, where the highest plant length is 56.16 cm in the F1 treatment. The best watering interval for the number of leaves of the plant is watering (once) a day, where the average number of leaves is 13.11 leaves in the F1 treatment. The best watering interval for plant height is watering once every 1 (one) day, where the average height of the highest plant is 51.16 cm in the F1 treatment.
Kode Etik Pengelolaan Limbah Bekas Dan Bahan Terkontaminasi Pada Laboratorium Kultur Jaringan Tanaman Athifah, Najwa Athifah; Sani, Ribi Zuhriah Sani; Yusuf Nasution, Mhd; Lubis, Khairiza; Situmorang, Nurbaity
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/rabwff67

Abstract

This study aims to describe the management of used culture media waste and contaminated materials, and to assess the understanding of laboratory staff and interns regarding ethical principles in plant tissue culture laboratories. A descriptive qualitative method was applied using observation, questionnaires, and literature review. The results indicate that most respondents have a good understanding, reflected in their agreement on the importance of sterilization, waste segregation, use of personal protective equipment, and implementation of standard operating procedures (SOP). Respondents are also aware of the risks of laboratory waste to health and the environment. Ethical practices are recognized as part of professional responsibility, but require improvement through training, supervision, and consistent SOP implementation.  
ANALISIS PENERAPAN PRINSIP BIOETIK PADA PENGELOLAAN LIMBAH B3 DILABORATORIUM KESEHATAN DAERAH KOTA BEKAS MAHENDRA, NAUFAL; NURISA SIDABUTAR, ELSHA; Situmorang, Nurbaity; Lubis, Khairiza
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/m7ztf382

Abstract

The management of hazardous and toxic waste (B3) in health laboratories remains a challenge, particularly inensuring regulatory compliance while consistently applying bioethical principles. This issue is important becauseimproper waste management can negatively impact the environment and public health. Therefore, this study aimsto analyze the B3 waste management system, its compliance with regulations, and the implementation of bioethicalprinciples at the Regional Health Laboratory (Labkesda) of Bekasi City. This study employed a descriptivequalitative method with a case study approach. Data were collected through interviews, observations, anddocumentation involving two informants directly engaged in waste management. In addition, the implementationof bioethics was assessed using a Likert scale with ten indicators. The results show that B3 waste management hasbeen carried out in a structured manner, including identification, containment, transportation, temporary storage,and processing by licensed third parties, thus complying with applicable regulations. Furthermore, theimplementation of bioethical principles achieved an average score of 4.8 out of 5, indicating a very good category.However, an obstacle was identified in the form of delays in waste collection by third parties. In conclusion,Labkesda Bekasi City has been able to manage B3 waste effectively and sustainably, although improvements inexternal coordination are still needed
Penerapan Prinsip Bioetik dalam Pengujian Escherichia coli pada Air dan Salmonella pada Ikan Nila (Oreochromis niloticus) Menggunakan Media Compact Dry Berdasarkan Standar ISO/IEC 17025 di Laboratorium BPPMHKP Medan I Andina Ayu Syahputri; Dara Arifia, Dini; Situmorang, Nurbaity; Yusuf Nasution, Mhd; Arwita, Widya
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/xk28gj62

Abstract

Food safety and environmental quality are critical aspects in maintaining public health, particularly in fishery products and water sources that are vulnerable to contamination by pathogenic microorganisms. This study aims to identify the presence of Escherichia coli in water samples and Salmonella in tilapia (Oreochromis niloticus), as well as to analyze the use of Compact Dry media based on ISO/IEC 17025 and the implementation of bioethical principles in microbiological testing. This research employed a descriptive laboratory approach conducted at the BPPMHKP Medan I Laboratory using Compact Dry media based on AOAC methods. The samples consisted of lake water and tilapia, which were tested through enrichment, inoculation, incubation, and colony observation stages. The results showed no growth of Escherichia coli colonies on Compact Dry EC media and no Salmonella colonies on Compact Dry SL media, indicating that all samples were negative for the tested bacteria. The use of Compact Dry media in this study complied with ISO/IEC 17025 standards, as indicated by systematic procedures, the use of validated media, and laboratory quality control. Furthermore, bioethical principles, including scientific integrity, responsibility, biosafety, and accountability, were applied throughout all stages of testing. Therefore, this study demonstrates that the use of validated media and the application of bioethical principles play an important role in ensuring valid, reliable, and accountable laboratory results.
Co-Authors Alfina Siska Dewi Andina Ayu Syahputri Athifah, Najwa Athifah Baringbing, Siska Juliana ButarButar, Yutrina Dalimunthe, Aina Mardiah Damaiyani, Damaiyani Dara Arifia, Dini Denisa, Rifka Depari, Nelfita Rizka S. Dewi Novina Sukapiring, Dewi Novina Dini Novita Sari E. P, Chatryn Joice Elisabet, Melfa Romanna Endang Sulistyarini Gultom Ester Ester Fadhilah, Zahra Fauzi, Muhammad Dahlan Fauziah, Ennita Febriantika, Febriantika Ginting, Silvia Margaretta br Gultom, Wirda Resinta Hariati, Emy Huda Panggabean, Nurul Husniatunnisa, Husniatunnisa Indah Anggraini, Jihan Jefri Anjaini Kaban, Hairen Br Kairani, Anita Kusumawati, R Lia Lestari, Dian Putri Lily Novianty Liu, Chun-Hung Loise Tarigan, Beatrice Lubis, Khairiza Lumbantoruan, Lamtiurma Mahendra, Naufal Malinda, Leni Manurung, Dhea Amelia Mega Utami Mirza, Mirzan Hasibuan Mustasfa, Sinta Wulida Mutia, Diska Qolbu Nasution, Ahmad Faisal Nasution, Muhammad Yusuf Nasution, Putri Nurlela Ndaha, Gebyta Yani NIZMI PUTRI Nura S, Alfi NURISA SIDABUTAR, ELSHA Nurkhofifah, Indah Pandiangan, Putri Patricia Panggabean, Nurul Huda Pasaribu, Maria Benedikta Portuna, Dewi Pulungan, Ahmad Shafwan Purba, Melpa Yusmawati Rahmadina, Rahmadina Rahmat Suryanto Pirngadi Ramadhan, Nuri Rangkuti, Marlinda Nilan Sari Rindiani, Artika Rini Hafzari Saiful Amir Salsabilah, Talita Sani, Ribi Zuhriah Sani Saragih, Elsa Angelina Saragih, Putri Fransiska Saragih, Yuni Darmawati Sarah, Oenih May Sari, Yusdiah Sastra, Siti Fathiya Dwinda Selvia Dewi Pohan Sembiring, Juhardi Shafwan Pulungan, Ahmad Siahaan, Eliza Sriyuliani Simanjuntak, Risda Sinaga, Fahri Siti Khairani Siti Khairani SITI NURLANI harahap Situmorang, Elisa Rauli Afrilia Sriyuliani Siahaan, Eliza Suharsih Sukma, Nurhaliza Syafwan Pulungan, Ahmad Tamba, Gelora Betania Tohap Simangunsong Triana, Rennyta Utami, Khairuna Virginia Tamara , Chatrine Wahyu Hidayat Waliid Naufal Widya Arwita wulandari, atika Yudha Pratama, Rizky Yulina, Nia Yusdiah Sari Yusuf Nasution, Mhd Zain, Ulfi Khairani