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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Ilmiah Mahasiswa Veteriner INVOTEK: Jurnal Inovasi Vokasional dan Teknologi The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria

Darmawi Darmawi

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Author-ID : 2567720

Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Transportation Veterinary Other

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2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA Siti Hajar; Safika Safika; Darmawi Darmawi; Wenny Novita Sari; Erdiansyah Rahmi; Yandi Syahputra; Nurliana Nurliana; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.

The Effect Of Cellulase Content On Phytoestrogens Formation (Genistein) Materials On Fermented Soybean Waste Aspergillus niger Vara Tassa Sutari; Nurliana Nurliana; Sugito Sugito; Darmawi Darmawi; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P 0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.

The Presence of Salmonella in Sie Balu, Acehnese Dried Meat After Gamma Irradiation Husna Husna; Nurliana Nurliana; Darmawi Darmawi
The International Journal of Tropical Veterinary and Biomedical Research Vol 2, No 1 (2017): Vol. 2 (1) May 2017
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Sie balu is an Acehnese dried meat preserved by the addition of salt, acid and dried. However, long processing and drying it under the sun can cause meat products contaminated by Salmonella. Irradiation can eliminate Salmonella and other pathogenic bacteria in food. This study aims to determine the presence of Salmonella in sie balu after irradiated with increasing doses and 2 to 4 months shelf life. Sie balu was made of 5 kg fresh beef, dried under the sun to dry, vacuum and irradiated at doses of 5, 7 and 9 kGy. Salmonella was detected using bacteriological and biochemical tests. Results showed sie balu contaminated by Salmonella paratyphi B and Salmonella cholerasuis. The irradiation and shelf life significantly affect (P0.05) the count of Salmonella in sie balu compared with unirradiated one. Irradiation doses of 5, 7 and 9 kGy do not affect the count of Salmonella in sie balu. Extending the shelf life up to 4 months can increas the count of Salmonella. This study concluded that irradiated sie balu at doses of 5, 7 and 9 kGy can be stored for up to two months

8. Histopathological of Fabricius Bursa in the Broiler Chicken after Vaccination with Newcastle Disease Virus Vaccine Ummu Balqis; Nurjannah Annah; Hamdani Budiman; Darmawi Darmawi; Cut Dahlia Iskandar; M. Hasan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4178

The aim of this research was to determine an effect of Newcastle disease virus (NDV) vaccine to histopathologic on Fabricius bursa of broiler chicken at various age levels. Eighteen commercial DOC (Day Old Chick) were divided into 2 groups namely a control group that was given distilled water and a treatment group that was given NDV vaccine namely ND Lasota vaccine ocularly as much one drop per eye at day 3, then a booster dose in drinking water at day 13 was given. The bursa of Fabricius was collected from these chickens on the age of 14 days, 21 days, and 28 days after sacrificing the chickens through necropsied, then histopathological preparations are made using hematoxylin-eosin staining. The parameters observed were measured the number, diameter, and thickness of cortical of lymphoid follicles. Data were analyzed descriptively and quantitatively by unpaired t-test. The results of this research showed the NDV vaccine effects were not significantly different (P0.05) to the number and diameter of lymphoid follicles. In the treatment group, 14 days old broiler chickens had a decreased number of lymphoid follicles, while its diameter tends to grow and the thickness of cortex was also seemed unchanged compared with the control group. Then at the age of 21 days, the number, diameter, and thickness of cortex of lymphoid follicles tend to increase, while at 28 days diameter of lymphoid follicles tend to be slightly reduced, but the number and thickness tend to grow out of control. It was concluded that the NDV vaccine had a good effect on the Fabricius bursa of broiler chickens with increasing age, then histopathological preparations are made using hematoxylin-eosin staining.

9. The Isolation of Steptococcus sp on horse’s dental caries in Aceh Tengah Regency Sagasias Ahmad; Erina Erina; M Daud AK; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; M. Isa
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4218

The purpose of this study was to detect the existence of the Streptococcus sp bacteria in the horses’ dental caries in Aceh Tengah Regency. The caries samples used in this study were taken from 8 horses in Aceh Tengah Regency. The samples were collected using swab on the teeth and then put into Nutrient Broth (NB) media and further were cultured on selective media TSY20B. The bacteria identification was conducted by observing the bacteria’s morphology, Gram staining and biochemical test. The eight samples of the horses’ dental caries showed that Streptococcus sp bacteria could be isolated from all of the collected samples. From this research it could be concluded that Streptococcus sp bacteria was found in the horses’ dental caries and it was one of the causes of the dental caries.

9. Total colony of cellulolitic bacteria in the rumen of aceh cattle Safika Safika; Siti Wardianah Matondang; Darmawi Darmawi; Mahdi Abral; Erina Erina; M Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.3411

The aim of this study was to count the total sum of cellulolitic bacteria colony in aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml

5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar Erina Erina; Mahdi Abrar; Balqis Alrasyid Suyoto; Maryulia Dewi; Darmawi Darmawi; Darniati Darniati; Muttaqien Bakri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.3986

This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar

10. Isolation and identification of Aeromonas sp in goldfish (Carrasius auratus) from several pet shops in Banda Aceh Hamny Hamny; Muhammad Zulfan; Mahdi Abrar; Maryulia Dewi; Darmawi Darmawi; Razali Daud; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4225

The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.

10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera Resti Reimena; Erina Erina; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Darmawi Darmawi; Hamdani Budiman
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.3524

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo

PENGARUH PENCELUPAN KARKAS AYAM PEDAGING DALAM LARUTAN ASAM SITRAT DAN ASAM ASETAT TERHADAP ANGKA LEMPENG TOTAL Escherichia coli (The Immersion Effect of Broiler Carcass in Citric Acid and Acetic Acid on the Total Plates Count of Escherichia Coli) Nurliana Nurliana; Sandy Cakra Yuda; Faisal Jamin; Teuku Reza Ferasyi; M. Isa; Darmawi Darmawi
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3812

This study aimed to determine the immersion effect of broiler carcasses in 3% citric acid, 3% acetic acid, and combination of citric acid and acetic acid with concentration of 1.5% each on the total plate count of bacteria E. coli. Eight broilers carcasses (dissected into 2 parts) were divided into 4 treatment groups with 2 times repetition and 3 times observation at 0, 4, and 8 hours after dipped. The control treatment (K0) without dipped into acid solution. Broiler carcasses in the first (K1), second (K2), and the third (K3) treatments were dipped into 3% acetic acid, 3% citric acid, and combination of 1.5% acetic acid and 1.5% citric acid respectively. Data were analyzed using analysis of variance. The average of E. coli count in K0 at 0, 4, and 8 hours post dipping were 5.2±0.061, 6.8±0.99, and 7.0±0.107 log 10 cfu/g of chicken meat respectively. In similar observation time with K0, the average of total E. coli count were 5.5±0.373, 6.0±0.023, and 6.0±0.242 log 10 cfu/g of chicken meat in K1, 5.3±0.166, 6.0±0.143, and 6.0±0.084 log 10 cfu/g of chicken meat in K2, 5.7±0.041, 5.9±0.92, and 5.8±0.058 log 10 cfu/g of chicken meat in K3 at 0,4, and 8 hours post dipping respectively. Statistical analysis showed that the immersion of broiler carcass in acetic acid and citric acid solution were significantly (P 0.05) decrease the number of E. coli. There were interaction between immersion of broiler carcass in organic acids and observation time which significantly decrease the number of E. coli (p 0.05). In conclusion, the immersion of carcass chicken in 3 % acetic acid, 3% citric acid, and their combination reduce the amount of E. coli. Key words: chicken carcass , citric acid 3%, acetic acid 3% , Escherichia coli

Co-Authors . Darniati . Nurliana Abdullah Hamzah Al Azhar Amalia Sutriana Annisah putri Ananda Nasution Azhar Azhar Azhari Azhari Balqis Alrasyid Suyoto Borgo Mauly Nasution Budianto Panjaitan Cut Dahlia Iskandar Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrazi Fakhrurrarzi Fakhrurrazi Fakhrurrazi Feby Fema Amzani Nasution Hamdani Budiman Hamny Sofyan Husna Husna Ismail Ismail laras berlian xarolin M Daud AK M Jalaluddin M. Hasan M. Isa Mahdi Abral Mahdi Abrar Mahdi Abrar Maryulia Dewi Masda Admi Muhammad Hambal Muhammad Jalaluddin Muhammad Zulfan Muttaqien Bakri Nurdin Hendri Nurjannah Annah NURLIANA NURLIANA Rastina Rastina Razali Daud Razali Razali Resti Reimena rina dwita Rinidar Rinidar Safika S, Safika Sagasias Ahmad Sandy Cakra Yuda Siti Hajar Siti Wardianah Matondang Sugito Sugito Sugito Sugito Teuku Reza Ferasyi Teuku Reza Ferasyi Teuku Zahrial Helmi Ummu Balqis Usma Aulia Vara Tassa Sutari Waskito Waskito Wenny Novita Sari Winaruddin Winaruddin Yandi Syahputra

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