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Journal : BIOEDUKASI

ESCHERICHIA COLI BACTERIA DETECTION IN DRINKING WATER DEPOTS BUNGO DISTRICT Titis Wulandari; Randi Eka Putra; Tri Wera Agrita
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.25405

Abstract

Water is a very important component in life, such as the use of water as drinking water, all living things, both humans, animals, and plants, need water. Part of the human body consists of fluids, so water helps the body to work to carry nutrients from the stomach to all parts of the body. So that a hygienic water source is needed, through a processing process first until the water meets health requirements. The purpose of this study was to detect the presence of Escherichia coli bacteria in drinking water depots in two sub-districts in Bungo Regency, namely (Rimbo Tengah, Pasar Muara Bungo). The test was carried out at the Fish Quarantine Station and Quality Control Laboratory of Class 1 Jambi. Direct sampling with sterile bottles. The sample testing method uses the Most Probable Number (MPN) test with SNI ISO 9308-1:2014. Direct sampling with sterile bottles. The sample testing method uses the Most Probable Number (MPN) test with SNI ISO 9308-1:2014.
TECHNIQUES OF PURIFICATION of ANTIGEN O ISOLATES of Escherichia coli AND IN VIVO ANALYSIS OF ANTIGEN IMMUNOGENICITY IN POULTRY Achmad Bahtiar Rifai; Anita Yuni Praptiwi; Dwi Sulistiyono; Titis Wulandari
BIOEDUKASI Vol 21 No 1 (2023)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v21i1.28167

Abstract

Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and is recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection against infection and vaccines have been developed by conjugating O antigens to carrier proteins to enhance immunogenicity. This study aims to produce O antigen from Escherichia coli and analyze the in vivo test results in poultry using ELISA technique. The methods used in this study were E. coli Bacterial Culture, O Antigen Purification, SDS PAGE, Inoculation on Experimental Animals, Rapid Plate Agglutination Test, and ELISA. Based on the results of the research conducted, it can be concluded that the O antigen of Escherichia coli injected into chickens can induce the formation of specific antibodies against E. coli in the blood. Specific antibodies to E. coli formed can be detected using the rapid plate agglutination test and ELISA technique.
Co-Authors Achmad Bahtiar Rifai agrita, tri wera Agus Fadila Ajis Agustin Indrawati Ajis, Agus Fadila Al-Ihsan, M.Iqbal Aldino, . Andromeda Andromeda Anita Yuni Praptiwi Apdoludin Apdoludin, Apdoludin Aprizan Aprizan, Aprizan Arif Rahman Arisman Sabir Aryani, Fara Dila Dwi Asrizai Asrizai Asrizai Asrizai Asrizal AVANA, NURLEV Avana, Nurlev Chindi Yuliana Dedek Helida Pitra Dedy Rahmat Saputra Desnita Dwi Prasetyo, oktavian Dwi Sulistiyono Eli Rohaeti Evi Susanti Fachriyan Hasmi Pasaribu Faozia, Firda Fathuroh, Siti Fatni Mufid Febriansyah Festiyed Festiyed Festiyed Fitri Arsih Gian Rahmat Sahasra Gunaria Siagian Habibi, Zirul Habibie, Zulqoidi R. Hakiki, Muhammad Hana Catur Wahyuni Hardeli Hardeli Hardeli Hardeli harkam tujantri Helvina, Nadia Hermana, Nabila Swarna Puspa Hidayati R Ikhsan Maulana Putra Ikhsan Maulana Putra Ilwandri Insira Insani Fitri Irawanto, Pramudya Dwi Wahyu Irham Ramadhani JHONY HENDRA, GHAZALI INDRA PUTRA Kurniadi, Muhammad Dwi Lufri Lufri Lufri, . M Iqbal Al Ihsan M Iqbal Al-ihsan M. Iqbal Al-Ihsan Mailisa, Riski MORI SAPUTRA, DEKA ISMI Muhammad Irhas, Muhammad Muhammad Sabandi Nanich Framulya Nico Wahyu Saputra Oktavian Dwi Prasetyo Oktavian Dwi Prasetyo Pradewi, Okantita Gangga Prasetyo, Oktavian Dwi Puspita Handayani Putra, Ikhsan Maulana Putra, Randi Eka R, Hidayati Rifai, Achmad Bahtiar Riska Riska Riska Riska Sabir, Arisman Saputra, Andika Dimas Septianingtyas, Maya Cobalt Angio Siagian, Gunaria Siti Fathuroh Skunda Diliarosta Surastini, Desi Tri Wiyoko tujantri, harkam Yerimadesi Yerimadesi Yul Ifda Tanjung Yul Ifda Tanjung yuliana, chindi Yuni Ahda Zirul Habibi Zirul Habibi