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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Bioma : Berkala Ilmiah Biologi MPI (Media Pharmaceutica Indonesiana) JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Indonesian Journal of Chemical Science Science and Technology Indonesia KLOROFIL: Jurnal Ilmu Biologi dan Terapan Fullerene Journal of Chemistry Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) CERATA Jurnal Ilmu Farmasi Jurnal Ilmu Kesehatan BIO-EDU: Jurnal Pendidikan Biologi Jurnal Pengabdian Kesehatan Jurnal Ilmiah Jophus: Journal of Pharmacy UMUS Community Empowerment KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Farmasi dan Sains Indonesia (JFSI) Jurnal Pengabdian Kepada Masyarakat Journal Of Research and Education Chemistry (JREC) Prosiding University Research Colloquium Jurnal Bakti Untuk Negeri Jurnal Pengabdian Masyarakat Bangsa Sinteza Batik-Mu : Jurnal Pengabdian Masyarakat Molekul: Jurnal Ilmiah Kimia Benzena Pharmaceutical Scientific Journal CERATA Kartika : Jurnal Ilmiah Farmasi Indonesian Journal of Chemical Science Jurnal Sains dan Kesehatan Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal)
Khusna Santika Rahmasari
Program Studi Sarjana Farmasi, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Pekajangan, Pekalongan
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Determination of Phenolic Total, Flavonoid Total, Antioxidant Assay and Inhibition of Xanthine Oxidase Enzyme Activity Kemloko Fruits Extract (Phyllanthus Embilica. L) Wirasti Wirasti; Khusna Santika Rahmasari; Isyti'aroh
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 9 No. 1 (2024)
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/farmasains.v9i1.33737

Abstract

People use Kemloko Fruits as sweets and some also use it as a herbal medicine to reduce uric acid. The sour-tasting Kemloko fruit contains a lot of ascorbic acid. Ascorbic acid and ingredients contained in it have the effect of preventing or reducing uric acid levels. The aim of this research was to treat the antioxidant effect and inhibitory of the xanthine oxidase enzyme Kemloko Fruit extract, as well as determine the phenolic total and flavonoid total levels. The method used to make Kemloko fruit extract uses maceration with ethanol 96% solvent. The phytochemical screening method uses an in-tube reaction for flavonoid content using the Shinoda test, alkaloids using the Dregendorf reagent, saponins using the foam test and terpenoids/steroids using the Liebermann Burchard reagent. Meanwhile, the methods for determining phenolics and flavonoids total are the colorimetric used spectrophotometry UV-Vis, the DPPH methods for antioxidant assay, and measurenment of inhibiting xanthin oxidase activity using a microplate at a wavelength of 375 nm. Qualitative data produced by the ethanol extract of Kemloko fruit contains tannins, flavonoids, alkaloids, terpenoids, saponins and glycosides. Meanwhile, Quantitative data showed that the phenolic and flavonoid total levels were 1465.63±0.003 mgGAE/g extract and 9.52±0.001 µg/mL, respectively, as well as the IC50 for xanthin oxidase enzyme inhibition of 20.65 µg/mL and the IC50 antioxidant power of 9.49 ±0.0007 µg/mL. Kemloko fruit ethanol extract contains tannins, flavonoids, alkaloids, terpenoids, saponins and glycosides has inhibitor activity against xanthine oxidase and antioxidant is a strong.
Analysis of Rhodamin B on Lipstick, Blush On and Eye Shadow in Pekalongan Regency With Uv-Vis Spectrophotometer Rahmasari, Khusna Santika; Waznah, Urmatul
Jurnal Ilmu Kesehatan Vol 10 No 2 (2022): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30650/jik.v10i2.3342

Abstract

Rhodamine B is a synthetic dye that is often misused in cosmetic products. These dyes can cause irritation to the respiratory tract, are carcinogenic and cause liver damage in high concentrations. This study aims to determine the content of rhodamine B in lipstick, blush on, and eye shadow in the Pekalongan Regency. The analysis used in this research is qualitative and quantitative analysis. Qualitative study using Thin Layer Chromatography (TLC) with silica gel F254 as stationary phase and mobile phase n-butanol, ethyl acetate, ammonia (5.5 : 2: 2.5) and then detected on UV lamps at 254 nm and 366 nm. Quantitative analysis using UV-Vis spectrophotometer at a maximum wavelength of 557 nm. The results of the qualitative analysis showed that three samples of lipstick (sample A, D and E), two samples of blush on (sample B and C), and three samples of eye shadow (sample A, B and E) contained rhodamine B. Rhodamine B levels in the sample lipstick A 0.00244%, lipstick D 0.00052%, lipstick E 0.00065%, blush on B 0.00239, blush on C 0.01091%, eye shadow A 0.00057%, eye shadow B 0.00140, and eye shadow E 0.00047%.
Pengaruh Metode Pengolahan Terhadap Total Antosianin dan Uji Aktivitas Antioksidan Pada Jagung Ungu (Zea mays L. var. ceratina Kuleshov) Putriautami, Fernanda Meyra; Nur, Achamd Vandian; Rahmasari, Khusna Santika; Pambudi, Dwi Bagus
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i2.17237

Abstract

Purple corn is a plant belonging to the Poaceae family which has the Latin name Zea mays L. var. ceratina Kuleshov. Purple corn contains anthocyanins which are beneficial for human health as antioxidant agents. Anthocyanins have low stability at high heat and dissolve easily in water. The purpose of this study was to determine the total anthocyanin levels and antioxidant activity of fresh, steamed, and boiled purple corn. This research was conducted qualitatively using thin-layer chromatography with the mobile phase n-Butanol: ethyl acetate: distilled water (4:1:5) and quantitatively using the differential pH method and the antioxidant activity test using the DPPH method. The results obtained from the TLC test showed anthocyanin standard 0.72; fresh purple corn 0.71; steamed purple corn 0.71, and boiled purple corn 0.71. In the qualitative test of total anthocyanin levels using the differential pH method, the total anthocyanins in fresh purple corn were 235,45 mg/100 g; steamed purple corn of 38,908 mg/100 g, and boiled purple corn of 20,42 mg /100 g. From the antioxidant test with the DPPH method, it was obtained that the best IC50 value in fresh purple corn samples of 33.92 (very strong); steamed purple corn was 124.33 (medium) and boiled purple corn was 126.26 (medium).
Penetapan Kadar Cd2+ dan Pb2+ pada Ikan Mujair (Oreochromis mossambicus) dengan Metode AAS (Atomic Absorption Spectrophotometry Rahmasari, Khusna Santika; Safitri, Arista; Nur, Achmad Vandian; Wirasti, Wirasti
JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Vol 7, No 2 (2023)
Publisher : State University of Malang or Universitas Negeri Malang (UM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um0260v7i22023p001

Abstract

Ikan mujair (Oreochromis mossambicus) merupakan ikan yang tahan terhadap kondisi lingkungan dan mudah ditemukan yang apabila terpapar logam berat serta dikonsumsi dalam jangka panjang akan menimbulkan efek neurotoksik, karsinogenik, mutagenik ataupun teratogenik bahkan sampai kematian. Cd2+ dan Pb2+ merupakan logam berat berbahaya yang bersifat toksik serta dapat mencemari organisme perairan. Penelitian ini bertujuan untuk mengetahui kadar Cd2+ dan Pb2+ pada ikan mujair di tambak Kota Pekalongan. Sampel berasal dari tambak Jeruksari, Panjang, dan Slamaran. Analisis kadar logam berat menggunakan metode AAS (Atomic Absorption Spectrophotometry). Hasil penelitian menunjukkan ikan mujair di tambak Jeruksari, Panjang dan Slamaran mempunyai kadar Cd2+ sebesar 0,07; 17,38 dan 0,02 mg/Kg, serta kadar Pb2+ sebesar 10,26; 6,08 dan 8,62 mg/Kg. Kadar Cd2+ pada tambak Panjang melebihi ambang batas yang ditetapkan oleh BPOM yaitu 0,1 mg/Kg, sedangkan kadar Pb2+ pada ketiga tambak melebihi ambang batas yang ditetapkan oleh BPOM yaitu 0,2 mg/Kg.
Edukasi Pemanfaatan Toga Sebagai Pencegah Hipertensi Ningrum, Wulan Agustin; Waznah, Urmatul; Rahmasari, Khusna Santika; Slamet, Slamet; Balqis, Fadia Indah
Jurnal Pengabdian Kesehatan Vol 6, No 2 (2023): Jurnal Pengabdian Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) STIKES Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/jpk.v6i2.346

Abstract

TOGA (Tanaman Obat Keluarga) untuk pencegahan hipertensi bagi masyarakat. Persepsi masyarakat dalam menanggapi masalah hipertensi, persepsi yang sering keliru tentang pemanfaatan TOGA untuk pencegahan hipertensi pada masyarakat akan berdampak pada pola penerapan perawatan masyarakat dengan penyakit hipertensi. Tujuan dari kegiatan ini adalah untuk meningkatkan pengetahuan masyarakat khususnya ibu-ibu di Desa Bligo tentang pemanfaatan toga sebagai alternatif terapi pencegah hipertensi. Metode yang digunakan dalam kegiatan pengabdian masyarakat ini dibagi menjadi 3 tahap yaitu tahap persiapan, tahap pelaksanaan  program dan tahap evaluasi. Pelaksanaan kegiatan pengabdian masyarakat dilakukan di Desa bligo, sebagai peserta kegiatan ini ada ibu-ibu Asiyiyah Cabang Bligo Kabupaten Pekalongan. Hasil kegiatan ini menunjukkan adanya peningkatan pengetahuan masyarakat yang diketahui dari hasil post test sebesar 84,2% dan dari hasil kuisioner masyarakat yang menyatakan kegiatan ini sangat menarik.
Nano antioxidant serum ethanol extract lime peel (Citrus aurantifolia S.) Fortuna, Putri Asabella; Mugiyanto, Eko; Waznah, Urmatul; Rahmasari, Khusna Santika
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 7 No. 2 (2022)
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/farmasains.v7i2.35928

Abstract

Serum is a cosmetic that has received great attention from the industry, but the main challenges are appearance, transparency and public acceptance of herbal active ingredients. On the other hand, herbal cosmetics, such as those using lime peel, are also growing. This study aims to determine the antioxidant activity of lime peel made in a nano extract serum preparation. The method for making nano extracts uses ionic gelation with PSA (Particle Size Analyzer) and Zeta Potential characterization. The concentration of nano serum of lime peel ethanol extract used was 0.5%; 1%; and 3%. Meanwhile, the antioxidant activity testing method uses DPPH and data analysis applied one-way ANOVA then by Tukey test. The results of the nano extract serum formulation using organoleptic testing are transparent, thick and liquid, odorless, and feel moist on the skin. The results of nanoparticle characterization using PSA measurements with three replications were 295.1; 320.3; 385.4 nm and the results of testing the zeta potential of nanoparticles with three replications, namely 3.2; 3.4; 6.0mV. Furthermore, the results of the antioxidant activity test on the basis of the preparation formula are classified as weak (IC50 value of 156.22 μg/mL); Meanwhile, preparation formula with a concentration of 0.5%; 1%; 3% are considered very strong (IC50 value of 46.39 μg/mL; 41.06 μg/mL; 36.27 μg/mL). The results of data analysis consider significance of p value below 0.05. The conclusion is nano lime peel extract meets the requirements and the serum preparation meets the requirements and has antioxidant activity.
Antioxidant Release Profile from Chitosan/ĸ-Carrageenan-based Polyelectrolyte Complex Films as Active Packaging: A Preliminary Study Rahmasari, Khusna Santika; Ishartono, Bayu
Molekul Vol 19 No 3 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.3.8784

Abstract

Significant efforts are necessary to prevent the spoilage of fatty acid-rich foods as a result of lipid oxidation. Active packaging is a technology that employs packaging films containing antioxidants to increase the stability of perishable foods susceptible to oxidation. This preliminary study aimed to incorporate tannic acid (TA) into a chitosan/κ-carrageenan (Chit/κ-Car)-based polyelectrolyte complex (PEC) films and evaluate their antioxidant release profile. The composition of Chit/κ-Car, glycerol (gly), and TA in PEC films were also investigated in this preliminary study as factors influencing the mechanical properties of films and the antioxidant release profile. The prepared PEC films were identified by an FTIR spectrometer and examined through a mechanical property test. The DPPH method was used to assess the antioxidant property of the Chit/κ-Car-based PEC films. The infrared spectra revealed that PEC films were formed through the interaction between ꟷNH3+ of Chit and ꟷOSO3ꟷ of κ-Car. Increasing the κ-Car and TA compositions in the PEC films increased the tensile strength (TS) and water absorption capacity (WAC), but decreased the elongation (E). The release profile of TA from PEC films followed the Korsmeyer-Peppas model through the Fickian diffusion mechanism with n < 0.5. The antioxidant activity test of PEC films using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method revealed high antioxidant activity with radical scavenging activity (RSA) values up to 95.04%. As a result, the Chit/κ-Car-based PEC films have the potential to be developed as active packaging. Keywords: Active packaging, antioxidant, Chit/κ-Car-based PEC films, release profile, TA
AKTIVITAS PENGHAMBATAN α-AMILASE OLEH EKSTRAK DAUN BAKAU MERAH (Rhizophora stylosa) Ayu Dyah Purwati; Pambudi, Dwi Bagus; Wirasti; Khusna Santika Rahmasari
BENZENA Pharmaceutical Scientific Journal Vol 3 No 02 (2024): BENZENA PHARMACEUTICAL SCIENTIFIC JOURNAL
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/benzena.v3i02.5101

Abstract

Diabetes melitus merupakan salah satu penyakit degeneratif yang prevalensinya terus meningkat di Indonesia. Penelitian ini bertujuan untuk mengevaluasi potensi daun bakau merah (Rhizophora stylosa) sebagai agen antidiabetes alami melalui penghambatan enzim α-amilase. Ekstrak etanol daun bakau merah diperoleh melalui metode maserasi. Skrining fitokimia dilakukan untuk mengidentifikasi senyawa bioaktif. Aktivitas penghambatan α-amilase diuji menggunakan metode spektrofotometri dengan acarbose sebagai kontrol positif. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki aktivitas penghambatan α-amilase yang sangat aktif dengan nilai IC50 16,074 μg/mL lebih rendah dibandingkan acarbose (20,607 μg/mL). Skrining fitokimia mengungkapkan keberadaan alkaloid, flavonoid, fenol, saponin, steroid, dan tanin yang berpotensi berkontribusi terhadap aktivitas penghambatan α-amilase.Kata kunci: Rhizophora stylosa, penghambatan α-amilase, antidiabetes. 
Analisis Antibakteri Ekstrak Pacar Kuku (Lawsonia Inermis L.) Sthaphylococcus Aureus Dengan Metode Turbidimetri Nurul Janah, Fatimah; Nur, Achmad Vandian; Rahmasari, Khusna Santika; Mugiyanto, Eko
BIO-EDU: Jurnal Pendidikan Biologi Vol. 9 No. 3 (2024): BIO-EDU: Jurnal Pendidikan Biologi - December 2024
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v9i3.7925

Abstract

The leaves of henna are commonly used by local communities to treat burns, inflammation, and skin diseases. This plant contains lawsone (2–hydroxy-1,4-naphthoquinone) and various phenolic glycosides, flavonoids, tannins, alkaloids, and quinones, which are believed to contain antibacterial activity. This study aims to determine whether ethanol extracts from the leaves and stems of henna exhibit antibacterial activity and to assess their inhibitory effects against Staphylococcus aureus. The methods employed in this study include maceration and turbidimetric analysis. The extraction method was maceration with 96% ethanol as the solvent. The antibacterial activity was tested using the turbidimetric method with extract concentrations of 15%, 25%, and 30%. Chloramphenicol was used as a positive control, and NaCl served as a negative control. The results revealed that the leaf extracts of henna exhibited antibacterial activity in the leaves and stems of henna nails by comparing the average absorbance values before and after incubation. At concentrations of 15%, 25% and 30% of the leaves of henna na na experienced shedding after incubation which showed that the growth of bacteria had been inhibited. Meanwhile, in the henna nail stem extract, the concentration of 15% decreased after incubation and the concentration of 25%, 30% showed an increase in absorbance value after the incubation process.
ANALISIS KANDUNGAN BAHAN KIMIA OBAT NATRIUM DIKLOFENAK PADA SEDIAAN JAMU PEGAL LINU YANG BEREDAR DI KABUPATEN PEKALONGAN DENGAN METODE KCKT (KROMATOGRAFI CAIR KINERJA TINGGI) Ayu Setyowati; Achmad Vandian Nur; Slamet Slamet; Khusna Santika Rahmasari
Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Vol 13 No 1 (2022)
Publisher : UNIVERSITAS BHAMADA SLAWI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36308/jik.v13i1.315

Abstract

Jamu pegal linu merupakan salah satu produk obat tradisional yang banyak diminati oleh masyarakat, dapat menghilangkan pegal linu, nyeri otot, tulang dan dapat memperlancar peredaran darah. Bahan kimia obat natrium diklofenak merupakan obat yang digunakan sebagai obat anti nyeri tetapi disalahgunakan oleh produsen jamu untuk menambah efek pada produk jamunya agar mempunyai efek yang cepat. Tujuan dari penelitian ini untuk menganalisis sediaan jamu pegal linu yang beredar di Kabupaten Pekalongan dan untuk mengetahui kadar BKO natrium diklofenak dalamnya. Metode yang digunakan adalah uji kualitatif menggunakan KLT dengan fase diam silika gel F60 254 nm dan fase gerak etil asetat:n-heksana (9,5:0,5) dan uji kuantitatif menggunakan KCKT dengan fase gerak methanol:aquabidestilata (80:20). Hasil analisis kualitatif dengan KLT dari 14 sampel jamu pegal linu yang terdeteksi bahan kimia obat natrium diklofenak terdapat pada 6 sampel dengan nilai Rf natrium diklofenak 0,68 cm sedangkan pada sampel B, D, E, F, dan K diperoleh nilai Rf 0,65 cm dan sampel G diperoleh nilai Rf 0,68 cm. Sedangkan analisis kuantitatif dengan KCKT setiap kemasan 7 gram sampel B mengandung kadar sebanyak 3 mg, sampel D mengandung kadar sebanyak 0,69 mg, sampel E mengandung kadar sebanyak 2,11 mg, sampel F mengandung kadar sebanyak 1,18 mg, sampel G mengandung kadar sebanyak 1,55 mg dan sampel K mengandung kadar sebanyak 3,20 mg. Berdasarkan hasil penelitian yang diperoleh menunjukkan bahwa sampel jamu pegal linu yang beredar di Kabupaten Pekalongan dari 14 sampel jamu, 6 sampel mengandung natrium diklofenak.
Co-Authors A'yun, Qurrata Achmad Ridlo Achmad Vandian Nur, Achmad Vandian Adelia Fitriani Ainin, Shabrina Ainun Muthoharoh, Ainun Alfarizi, Muhammad Bahtiar ALIYAH, HIMATUL Allam, Faiq apt. Eko Mugiyanto, Eko Arum Dwi Nanda, Arum Atifah, Husnia Ayu Dyah Purwati Ayu Setyowati Azzahra, Nafisa Aufa Bagus Pambudi, Dwi Balqis, Fadia Indah Bayu Ishartono Bayu Ishartono Budi Utami citra_dinda Wardany Dwi Bagus Pambudi Eka Anydia Putri Eko Mugiyanto Eko Mugiyanto, Eko Elsa Septiana Khoirunnisa Fadhilah, Isna Fadillah, Rahma Ayu Fenilinas Adi Artanto Firman Faradisi Fitriani, Adelia Fortuna, Putri Asabella Fuadiya, Laila Azka Giani Khairunnisa, Cantika Haqiqi, Izzatul Huda, Muhammad Syahrian Ida Astuti Ida Astuti Indriyani, Puspa Irma Suryani Irsyad, Khabibullah Isyti'aroh Izzahra, Astrid Ika Kamilah, Rasya Rizqi Khoirunnisa, Aishwarya Khoirunnisa, Elsa Septiana Kholisatuz Zahro Mafruroh, Mafruroh Maharisti, Retno Aulia Maulvi, M. Arsyi Muhammad Bahtiar Alfarizi Muhammad Syahrian Huda Nadia Salsabila Ulfa Naila Izza Sabila Ningrum, Wulan Agustin Nirmala, Mutiara Suci Nunung Mufida Nunung Mufida Nur, Achamd Vandian Nurul Janah, Fatimah Oktaviani, Riska Kurnia Pambudi, Dwi Bagus Pambudi, Dwi Bagus Prastika, Sisca Windy Putriautami, Fernanda Meyra Qurrata A’yun Rahmatullah, ST. Rasya Rizqi Kamilah Retno Aulia Maharisti Rina Widhyani Rini Kristiyanti Riska Oktaviani S Slamet S Slamet, S Safitri, Arista Salsabila, Salma Fi Slamet Slamet . Slamet Slamet Slamet, Slamet Sugiharto, Sugiharto Supriyanto, Lukman Ulfa, Nadia Salsabila Ulhaq, M Daffa’ Dhiya’ Urmatul Waznah Vandian Nur,, Achmad Vanesa Maharani Vira Septiya W Wirasti Wibowo, Danang Novianto Wirasti Wirasti Wirasti Wirasti, W Wirasti, Wirasti Wulan Agustin Ningrum Yuvinda Devi Erviani Yuvinda Zahratulhakimah, Putri Balqis