Claim Missing Document
Check
Articles

Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria Sukmawati Sukmawati; Melda Yunita; Febrianti Rosalina
Indonesian Journal of Biology Education Vol 3, No 2 (2020): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.601 KB) | DOI: 10.31002/ijobe.v3i2.3237

Abstract

The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.
Edukasi Manfaat Yogurt Sebagai Salah Satu Probiotik Dan Metode Pembuatan Yogurt Sederhana Eka Astuty; Melda Yunita; Astuti Nur Fadhilah
JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM) Volume 4 Nomor 1 Februari 2021
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v4i1.3535

Abstract

ABSTRAK Yogurt adalah produk fermentasi bakteri dengan menggabungkan efek Lactobacillus delbrueckii subspesies bulgaricus dan Streptococcus salivarius subspesies thermophilus. Kaitan ilmiah antara probiotik dan manfaatnya bagi kesehatan manusia pertama kali diungkapkan oleh ahli mikrobiologi Rusia bernama Metchnikoff (1907). Ia menyatakan bahwa asam laktat yang dihasilkan oleh Lactobacillus dalam yoghurt dapat menghambat pertumbuhan beberapa spesies bakteri patogen.Masyarakat  perlu  mengetahui  lebih  dalam  mengenai  manfaat  yoghurt sebagai salah satu minuman probiotik . Yoghurt dapat diproduksi dalam skala besar maupun kecil baik untuk keperluan komersial maupun untuk konsumsi rumah tangga. Kegiatan pengabdian kepada masyarakat  ini  mencakup  kegiatan edukasi tentang manfaat  yoghurt  dan  cara  pembuatannya  kepada  masyarakat dan diharapkan masyarakat  dapat  meningkatkan  derajat  kesehatannya  secara  mandiri Kata Kunci :probiotik, yogurt, edukasi  ABSTRACT Yogurt is a bacterial fermentation product by combining the effects ofLactobacillus delbrueckii subspecies bulgaricus and Streptococcus salivarius subspecies thermophilus. The scientific link between probiotics and their benefits to human health was first revealed by a Russian microbiologist named Metchnikoff (1907). He stated that the lactic acid produced by Lactobacillus in yogurt can inhibit the growth of several species of pathogenic bacteria. People need to know more about the benefits of yogurt as a probiotic drink. Yogurt can be produced on a large or small scale both for commercial use and for household consumption. This community service activity includes educational activities about the benefits of yogurt and how to make it to the community and it is hoped that the community can improve their health status independently. Keywords: probiotic, yogurt, education
Analysis of escherichia coli dan salmonella sp. in shrimp fermentation products Sukmawati Sukmawati; Melda Yunita; Sipriyadi Sipriyadi; Erin D Noya; Henok Siboka Onim
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1785

Abstract

South Sorong, West Papua, is one of the areas that produce rebon shrimp. Rebon shrimp can be made into fermented products such as rebon shrimp paste (terasi) or acidic rebon shrimp paste (cincalok). Terasi is a food additive that functions as a seasoning or flavoring, while cincalok is a fermented food added with salt and rice. The objective of this study was to determine the contamination of E. coli and Salmonella in fermented rebon shrimp products. This study was a descriptive study by describing the presence or absence of contamination by E. coli and Salmonella sp. on rebon shrimp paste (terasi) and acidic rebon shrimp paste (cincalok). The study concluded that the samples of rebon shrimp paste (terasi) and acidic rebon shrimp paste (cincalok) in South Sorong were safe from contamination by Escherichia coli and Salmonella sp. According to the Indonesian National Standards Agency, fermented fish products with or without salt, good and suitable products for consumption are those that are negative for Salmonella, V. cholera, Escherichia coli with a limit of <3 MPN/g and Staphylococcus aureus should be <1 x103 colonies/g. 
Antibiotic susceptibility analysis of Escherichia coli as the cause of urinary tract infection Melda Yunita; Hasna Vuspita Latuconsina; Eka Astuty; Alessandra Flowrence Saija
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1849

Abstract

The incidence of urinary tract infection (UTI) in Indonesia is still quite high. The UTIs are generally treated with antibiotic. However, the use of antibiotics has recently increased tremendously thus triggering bacterial resistance. This study was to analyze the susceptibility of the Escherichia coli as the cause of the UTI to 15 antibiotics at the UPTD Health Laboratory Center of Maluku Province. The study was an experimental laboratory study using the disk diffusion method. The antibiotic disc was placed on the media and the inhibition zone against E. coli was measured. The results showed that the E. coli was resistant (26.67%) to 4 types of antibiotics, namely amoxilin, ampicillin, sulfametazole dan penicillin. Meanwhile, E. coli was sensitive (46.66%) to 7 types of antibiotics, namely kanamycin, gentamicin, subactam ampicillin, chloramphenicol, amikacin, ceffazedine and azithromycin. Furthermore, E. coli was intermediate (26.67%) against 4 types of antibiotics, namely ciprofloxacin, ceptriaxone, cefoxitin and colistin. This study concluded that of the 15 antibiotics tested, gentamicin, amikacin, and kanamycin were the most effective antibiotics in inhibiting the growth of E. coli that causes UTI. Nevertheless, the appropriate choice of antibiotic needs to be adjusted only by physicians based on local, regional and national patterns of susceptibility.
ANALISIS LEVEL PENGETAHUAN MASYARAKAT TERHADAP PERILAKU HIDUP BERSIH DAN SEHAT UNTUK MENCEGAH TRANSMISI COVID-19 Melda Yunita; Morgan Ohiwal; Eka Astuty; Sukmawati Sukmawati
JMM (Jurnal Masyarakat Mandiri) Vol 6, No 5 (2022): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v6i5.9875

Abstract

Abstrak: Serangan dari wabah novel coronavirus (Covid-19) telah menyebar secara masiv di seluruh wilayah Indonesia dan pernah menjadi kasus dengan angka kematian tertinggi di Asia tenggara. Berbagai upaya telah dilakukan oleh pemerintah bersama dengan pihak terkait untuk mencegah transmisi Covid-19 yang sangat cepat, termasuk dengan mempromosikan perilaku hidup bersih dan sehat (PHBS) pada seluruh lapisan masyarakat, khususnya di Desa Air Salobar Ambon. Tujuan dari kegiatan ini adalah untuk menganalisis level pengetahuan masyarakat mengenai PHBS untuk mencegah transmisi Covid-19. Kegiatan ini dilaksanakan pada 20 Maret 2021 di Desa Air Salobar dengan melibatkan 26 responden. Metode yang digunakan pada kegiatan ini ialah metode deskriptif yaitu dengan memberikan pretest, edukasi berupa penyuluhan PHBS, dan posttest kepada masyarakat. Hasil analisis menunjukkan bahwa nilai posttest meningkat dari 40,8% menjadi 95% setelah diberikan edukasi dengan rata-rata peningkatan nilai posttest dibandingkan pretest ialah sebesar 56.2%. Dapat disimpulkan bahwa edukasi membrikan dampak yang signifikan terhadap peningkatan level pengetahuan masyarakat mengenai PHBS setelah mendapat edukasi dari fasilitator.Abstract: The attack from novel coronavirus (Covid-19) outbreak has spread massively throughout Indonesia and has ever become the case with the highest death rate in Southeast Asia. Various efforts have been conducted by the government together with related parties to prevent the very rapid transmission of Covid-19, including by promoting clean and healthy living behavior (PHBS) at all levels of society, particularly in Air Salobar Village, Ambon. The objective of the community service activity was to analyze the level of knowledge of the community on PHBS to prevent the transmission of Covid-19. This activity was carried out on March 20, 2021 in Air Salobar Village by involving 26 respondents. The method used was a descriptive method, by providing pretest, education in the form of PHBS counseling, and posttest to the respondents. The results of the analysis showed that the posttest score increased from 40.8% to 95% after being given education with an increase in knowledge level of 56.2%.
EDUKASI RAGAM TANAMAN OBAT KELUARGA DI DUSUN MAHIA, MALUKU Eka Astuty; Elpira Asmin; Melda Yunita
JMM (Jurnal Masyarakat Mandiri) Vol 6, No 6 (2022): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v6i6.11055

Abstract

Abstrak:Tanaman Obat Keluarga (TOGA) pada hakekatnya adalah tanaman berkhasiat yang ditanam di lahan pekarangan yang dikelola oleh keluarga. Mitra kegiatan pengabdian masyarakat ini adalah masyarakat dusun Mahia. Tujuan dari kegiatan pengabdian masyarakat ini adalah untuk mengedukasi dan menganalisis tingkat pengetahuan masyarakat dusun Mahia tentang ragam tanaman obat keluarga. Edukasi disampaikan dengan metode ceramah menggunakan slide presentasi tentang jenis-jenis TOGA, manfaat TOGA, kemudian menampilkan video tata cara menanam TOGA serta pengelolaan juga pengolahan TOGA. Perbandingan hasil pre test dan post test pada kegiatan ini menunjukkan adanya peningkatan pengetahuan peserta tentang TOGA. Peningkatan pengetahuan tentang pengelompokan TOGA dan bagian tanaman yang dapat dijadikan obat adalah sebesar 38% sedangkan peningkatan pengetahuan tentang cara pengolahan TOGA adalah sebesar 34,3%. Evaluasi dalam bentuk post test meskipun bisa menunjukkan adanya peningkatan pengetahuan, namun peserta kegiatan belum bisa mengaplikasikan secara langsung pengetahuan yang diperoleh. Oleh karena itu, sebagai rencana tindak lanjut, tim pengabdian masyarakat merencanakan membuat kegiatan pengabdian masyarakat di kesempatan berikutnya tentang pembuatan simplisia dan budidaya TOGA.Abstract:Family Medicinal Plants (TOGA) are essentially nutritious plants that are planted in the yard managed by the family. The partners of this community service activity are the Mahia community. The purpose of this community service activity is to educate and analyze the level of knowledge of the Mahia community about the variety of family medicinal plants. The education was delivered using a lecture method using presentation slides about the types of TOGA, the benefits of TOGA, then showing a video on how to plant TOGA and the management and processing of TOGA. The comparison of the results of the pretest and posttest in this activity showed an increase in participants' knowledge about TOGA. The increase in knowledge about TOGA grouping and plant parts that can be used as medicine is 38%, while the increase in knowledge about TOGA processing is 34.3%. Evaluation in the form of a post test, although it can show an increase in knowledge, but the activity participants have not been able to directly apply the knowledge gained. Therefore, as a follow-up plan, the community service team plans to conduct community service activities in the next opportunity regarding the manufacture of simplicia and TOGA cultivation.
UPAYA PENCEGAHAN ANEMIA MELALUI DETEKSI DINI DAN PELATIHAN PEMERIKSAAN HEMOGLOBIN (HB) Rosdiana Mus; Presly G Siahaya; Dylan Tamalsir; Mutmainnah Abbas; Melda Yunita; Intania Riska Putrie; Titin Agustina
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 1 (2023): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i1.12019

Abstract

Abstrak: Masalah gizi yang paling umum dan sulit diatasi secara global yaitu anemia. Prevalensi kejadian anemia masih sangat tinggi terutama pada usia 5-14 tahun. Maluku termasuk salah satu provinsi dengan angka kejadian anemia yang masih tinggi. Deteksi anemia dapat dilakukan dengan melakukan skrining pemeriksaan hemoglobin secara berkala. Remaja putri pada umumnya memiliki pola diet tidak sehat dan dapat memicu risiko anemia. Sehubungan dengan hal tersebut perlu dilakukan kegiatan pencegahan anemia melalui deteksi dini dengan pemeriksaan dan pelatihan penggunaan alat sederhana pemeriksaan hemoglobin pada pihak sekolah. Kegiatan dilaksanakan pada 15 November 2022 di SMAN 3 Salahutu, Maluku tengah dengan jumlah peserta 142. Tahapan kegiatan dimulai dengan pendaftaran subjek, pengukuran tinggi dan berat badan dan pemeriksaan hemoglobin. Selain itu, memberikan pelatihan kepada guru untuk melakukan pemeriksaan Hb secara berkala. Hasil menunjukkan bahwa jumlah responden kelas X sebanyak 33, kelas XI sebanyak 61 dan kelas XII sebanyak 48 responden dengan rentang usia dari 14 hingga 19 tahun. Hasil pemeriksaan Hb menunjukkan nilai minimum pemeriksaan Hb ada 8, 24 g/dL dan maksimum 16,47 g/dL. Hasil kegiatan menunjukkan bahwa rata-rata responden mempunyai kadar hemoglobin normal.Abstract: The most common and difficult nutritional problem globally is anemia. The prevalence of anemia is still very high, especially at the age of 5-14 years. Maluku is one of the provinces with a high incidence of anemia. Anemia detection can be done by screening hemoglobin examination periodically. Adolescent girs generally have unhealthy diet patterns and can trigger the risk of anemia. In this regard, it is necessary to carry out activities to prevent anemia through early detection through Hemoglobin examination. The activity was held on 15 November 2022 at SMAN 3 Salahutu, Maluku Tengah with 142 participants. The activity phase began with subject registration, measuring height and weight and checking hemoglobin. In addition, providing training to teachers to carry out regular Hb checks. The results showed that the number of respondents in class X was 33, class XI was 61 and class XII was 48 respondents with an age range from 14 to 19 years. The results of the Hb examination showed that the minimum value of the Hb examination was 8.24 g/dL and the maximum was 16.47 g/dL. The results showed that the average respondent had a normal hemoglobin level.  
The potential of endophytic bacteria isolated from atung fruit seeds as antibacterial against skin pathogens Melda Yunita; Adelia Mantong; Halidah Rahawarin
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2271

Abstract

Antibiotic resistance is one of the biggest threats to global health today. Estimated by 2050, antibiotic resistance will cause around 10 million deaths per year if alternative treatments have not been identified. This study was aimed to determine the potential of endophytic bacterial isolates from the seeds of atung fruit (Parinarium glaberrimum Hassk) as an antibacterial against skin pathogens Cutibacterium acnes and MDR Staphylococcus aureus, and Staphylococcus epidermidis. This research was a descriptive observational research with laboratory experimental methods. This study used isolates of endophytic bacteria isolated from the seeds of atung fruit which were tested with pathogenic bacteria. The results showed that 2 of 7 endophytic bacterial isolates were able to inhibit the growth of C. acnes with clear zone diameter of 4 mm and 9 mm, respectively, yet couldn’t able to inhibit MDR S. aureus and S. epidermidis. This study concluded that the 2 isolates (isolate BBA5 and BBA9) could have potential as antibacterial against C. acnes. While 5 other isolates had not been able to inhibit the whole pathogenic bacteria tested. An optimization is necessary in an effort to develop these isolates as antibacterial.
Biochemical identification of endophytic bacteria isolates from medicinal plant kayu jawa lannea coromandelica (houtt.) merr Eka Astuty; Indas Wari Rahman; Melda Yunita
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2095

Abstract

Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.
UPAYA PENINGKATAN PEMAHAMAN MASYARAKAT MELALUI EDUKASI PEMANFAATAN LIMBAH RUMAH TANGGA SEBAGAI PUPUK ORGANIK Morgan Ohiwal; Melda Yunita; Saiful Alimudi; Usman Umarella; Sofia Mustamu; Safinah Yulianty Sitania; Yensy Lolita Salamor; Victor Leo Nolly Kewilaa
Jurnal Abdimas Sangkabira Vol. 3 No. 2 (2023): Jurnal Abdimas Sangkabira, Juni 2023
Publisher : Program Studi Diploma III Akuntansi Fakultas Ekonomi dan Bisnis Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdimassangkabira.v3i2.602

Abstract

Meningkatnya kebutuhan masyarakat diantaranya kebutuhan terhadap pangan menyebabkan sampah rumah tangga yang dihasilkan juga akan semakin besar. Hal ini jika tidak dikelola dengan baik tentunya akan menyebabkan pencemaran pada daerah sekitar pemukiman masyarakat yang akan berdampak terhadap kesehatan masyarakat itu sendiri dan juga lingkungan disekitarnya sehingga kegiatan edukasi kepada masyarakat perlu dilakukan. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masyarakat Desa Air Salobar Kelurahan Nusaniwe Kota Ambon dengan memberikan edukasi tentang pemanfaatan limbah rumah tangga menjadi pupuk organic. Kegiatan ini dilakukan di Desa Air Salaobar dengan melibatkan 21 respondent. Kegiatan dilakukan dengan beberapa tahapan: pemberian pretest, edukasi, dan posttest. Kegiatan berjalan lancar diikuti antusiasme masyarakat dengan peningkatan level pengetahuan sebesar 41.43%. Diharapkan masyarakat dapat terus memanfaatkan limbah rumah tangga menjadi pupuk organic untuk kebutuhan sendiri.
Co-Authors Abbas, Muthmainnah Ade Nurhayati Kusumadewi Adelia Mantong Alessandra Flowrence Saija Alimudi, Saiful Anggun Lestari Husein Astuti Nur Fadhilah ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Cucu Hodijah de Lima, Filda V.I. de Lima, Filda Vionita Irene Dilya Maghfirah Taher DRIANA, Elin Dylan Tamalsir Dylan Tamalsir Effendi Effendi Eka Astuti Eka Astuty Elpira Asmin Elpira Asmin Elpira Asmin Erin D Noya Ernawati Ernawati Esmeraldine, Genevieva Etrin Zulqarnain Eka Saputra Linggar Evangelista Risalia Haurissa Fadila Tri Starlia Farah Christina Noya Filda de Lima Grace Latuheru Halidah Rahawarin Hartono Nurlette Hasibuan, Andrian Bayo Arif Hasna Vuspita Latuconsina Hataul, Is Asma’ul Haq Henok Siboka Onim Hursepuny, Valentine Husein, Anggun Lestary Ilsan, Noor Andryan Indas Wari Rahman Inggrain, Maulin Inggraini, Maulin Intania Riska Putrie, Intania Riska Irwan Ivanmorl Ruspanah Jennifer Vivian Kiriwenno Jepri Agung Priyanto, Jepri Agung Johan B Bension Johan Bruyif Bension Kusnara, Hasti Pramesti Latuheru, Grace Lesbatta, Kezia Josawel Mailoa, Joice Mainase, Josepina Makatita, Miftah H Marasabessy, Rendi Marasabessy, Rendy Mariyatul Qibtiyah Maxwell Landri Vers Malakauseya Metekohy, Meilani Mus, Rosdiana Musaid, Hendra Mutmainnah Abbas Myllisa Citra Wulandari Nahumarury, Salmin Nahumarury, Salmin Safril Noviyanter Siahaya Noya, Farah C Nurdin, Sukmawati Nurul Kusuma Dewi Ohiwal, Morgan Patteilohy, Merry Presly G. Siahaya Rachmawati Dwi Agustin Rahawarin, Halidah Ramanda, Viqih Ramla, Razita Ratau, Asria Reubun, Ririn Jihan S Rosalina, Febrianti Rumatiga, Masdar Ruth Magdalena Lumbantobing Safinah Yulianty Sitania Sipriyadi Siti Nur Azizah Siti Nurfajriah Sofia Mustamu Soumena, Rifah Zafarani Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati, Sukmawati Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Talakua, Gratia Tamtelatihu, Carmila L Titin Agustina Titin Agustina Umban Adi Jaya Usman Umarella Valentine Hursepunny Victor Leo Nolly Kewilaa Vina Z. Latuconsina Warella, Juen Carla Yensy Lolita Salamor Yosephina Manse Yuliawati , Sri Yuniasih Mulyani Jubeliene Taihuttu