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Efektivitas Larutan Kultur Unsur Hara dengan Penambahan Agar Sebagai Media Evaluasi Padi Dalam Kondisi Toksisitas Besi Turhadi; Miftahuddin; Hamim; Munif Ghulamahdi
BIOEDUSCIENCE Vol 5 No 1 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.189 KB) | DOI: 10.22236/j.bes/516395

Abstract

Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida’s Half-Strength solution (HSY), Yoshida’s Half-Strength + 0.2% agar solution (HSYA), and Yoshida’s Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress . Turhadi; . Hamim; Munif Ghulamahdi; . Miftahudin
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

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Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Trap Culture and Colonization of Arbuscular Mycorrhizal Fungi from Corn Roots in Tidal Swamps Using Several Host Plants Marlin Sefrila; Munif Ghulamahdi; Purwono Purwono; Maya Melati; Irdika Mansur
Caraka Tani: Journal of Sustainable Agriculture Vol 38, No 1 (2023): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v38i1.70180

Abstract

Arbuscular mycorrhizal fungi (AMF) are mycorrhizal from the endomycorrhizal group. The fungi live on higher plants' roots by performing symbiotic mutualism. This study aimed to identify AMF spores after trapping in corn roots and the degree of root infection by AMF in several host plants. The study was conducted using tidal swamps soil samples taken from Mulyasari Tanjung Lago Village, Banyuasin Regency, South Sumatra. The experiment used a randomized block design and three replicates with four host plants as a treatment: corn, soybean, sugarcane (monoculture) and sugarcane-soybean (combination). The results showed that AMF spores found on corn roots after trapping were from the Acaulospora sp. and Glomus sp. groups. Hyphae, vesicles, arbuscules and spores are AMF structures found in the roots of host plants infected by AMF. The percentage of AMF infection in host plant roots ranged from 1.11% to 77.44% where the highest was in maize host plant roots at 77.44% in the form of internal hyphae. The maize host plant has a high potential to be colonized by AMF compared to a mixture of soybean, sugarcane and sugarcane-soybean.
Efektivitas Larutan Kultur Unsur Hara dengan Penambahan Agar Sebagai Media Evaluasi Padi Dalam Kondisi Toksisitas Besi Turhadi Turhadi; Miftahuddin Miftahuddin; Hamim Hamim; Munif Ghulamahdi
BIOEDUSCIENCE Vol 5 No 1 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/516395

Abstract

Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida's Half-Strength solution (HSY), Yoshida's Half-Strength + 0.2% agar solution (HSYA), and Yoshida's Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.
Sebaran potensi kandungan zinc galur-galur padi biofortifikasi berdasarkan warna beras pecah kulit Wage Ratna Rohaeni; Untung Susanto; Willy Bayuardi Suwarno; Trikoesoemaningtyas Trikoesoemaningtyas; Munif Ghulamahdi; Hajrial Aswidinnoor
Jurnal AGRO Vol 10, No 1 (2023)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/24518

Abstract

Zinc (Zn) is an essential micronutrient that is very useful for plants as well as humans. Breeding high zinc rice varieties and pigmented varieties will increase the added nutritional value of the rice consumed. Knowing the distribution of zinc content based on pigmented rice is necessary to see the opportunities for obtaining pigmented rice lines with high zinc. This study aimed to determine the potential distribution of zinc based on the pigment of brown rice. The research was carried out at the Breeding Laboratory of the Indonesian Center for Rice Research from February to July 2022. A total of 970 lines derived from various biparental crosses and 10 varieties were used as research material. The samples were brown rice from a field research plot arranged in an augmented design with 5 blocks. Analysis of zinc content used the X-ray Fluorescence method using the XRF Machine Supreme8000. The results showed that there was a significant difference in Zn content based on CPC color. White rice had the widest range of zinc content compared to brown and black rice. The maximum zinc content found in white rice was 55.82 mg kg-1 and the maximum yield potential was 138.29 g per hill. The black rice genotypes in all categories had a zinc content exceeding the Inpari IR Nutri Zinc potency. Black rice type C was the one with the widest variety. Types of black rice with high zinc content and high yield potential are Black B and Black E types. ABSTRAKZinc (Zn) merupakan mikronutrisi esensial untuk tanaman dan juga manusia. Perakitan varietas  padi zinc tinggi pada beras-beras berwarna akan meningkatkan nilai tambah gizi beras yang dikonsumsi. Keragaman kandungan zinc berdasarkan warna beras perlu diketahui untuk melihat peluang diperolehnya varietas beras berwarna dengan keunggulan zinc tinggi. Penelitian ini bertujuan untuk mengetahui potensi zinc berdasarkan warna beras pecah kulit. Penelitian dilaksanakan Laboratorium Pemuliaan BB Padi dari bulan Februari sampai Juli 2022. Sebanyak 970 galur hasil persilangan biparental dan 10 varietas digunakan sebagai materi penelitian. Sampel berupa beras pecah kulit dari plot penelitian lapangan dengan rancangan augmented 5 blok. Analisa kandungan zinc menggunakan metode X-ray Fluorescence mesin XRF Machine Supreme 8000. Hasil menunjukkan bahwa terdapat perbedaan nyata kandungan zinc berdasarkan warna BPK. Beras putih memiliki rentang kandungan zinc yang paling luas dibandingkan beras merah dan hitam. Potensi zinc maksimum terdapat pada beras putih dapat mencapai 55,82 mg kg-1 dan potensi maksimum hasil 138,29 g per rumpun. Genotip padi semua kategori tipe beras hitam memiliki rentang kandungan zinc di atas potensi Inpari IR Nutri Zinc. Tipe beras Hitam C adalah beras yang memiliki varian paling luas. Tipe beras hitam yang memiliki peluang kandungan zinc sangat tinggi dan potensi hasil tinggi adalah tipe Hitam B dan Hitam E.
Efektivitas Larutan Kultur Unsur Hara dengan Penambahan Agar Sebagai Media Evaluasi Padi Dalam Kondisi Toksisitas Besi Turhadi Turhadi; Miftahuddin Miftahuddin; Hamim Hamim; Munif Ghulamahdi
BIOEDUSCIENCE Vol 5 No 1 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/516395

Abstract

Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida's Half-Strength solution (HSY), Yoshida's Half-Strength + 0.2% agar solution (HSYA), and Yoshida's Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.
Pengaruh pemupukan P dan populasi jagung dengan tumpang sari kedelai pada budidaya jenuh air di lahan pasang surut Faadhilah, Fajar; Lubis, Iskandar; Ghulamahdi, Munif
Journal of Agrosociology and Sustainability Vol. 1 No. 2: (January) 2024
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/jassu.v1i2.2024.309

Abstract

Soybean and corn are the main food crops in Indonesia after rice. Currently to fill the needs of national soybeans and corn obtained through imports. That was due to the low productivity of soybeasn and corn in Indonesia compared to other countries like as the United States and China. Iimproving the productivity of corn and soybean is by regulating plant population and fertilizing. The purpose of this research was to determine the effect of P fertilizing and corn population on soybean and corn intercropping in tidal swamps. This research was in March to August 2019 in Karyabakti, Rantau Rasau District, Tanjung Jabung Timur Regency, Jambi Province. The tidal swamps type used was type B. The study was conducted using two factors i.e P fertilization (0 kg P2O5 ha-1, 46 kg P2O5 ha-1, 92 kg P2O5 ha-1, 138 kg P2O5 ha-1) and population maize (50,000, 63,000 and 73,000 plants ha-1). The intercropping system showed the highest results in a population of 63,000 maize ha-1 plants with a fertilizer dosage of  P 46 kg P2O5 ha-1. Land equality ratio highest 2.3 showed that intercropping systems were 130% more effective than monoculture planting.
Utilizing Rice Hull Ash and Biomass as Ameliorants Enhanced Soybean Yield and Nutrient Uptake in Tidal Swamps Under Saturated Soil Culture Toyip, Toyip; Ghulamahdi, Munif; Sopandie, Didy; Aziz, Sandra Arifin; Sutandi, Atang; Jarwadi Purwanto, Mohamad Yanuar
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18486

Abstract

Saturated soil culture (SSC) is a promising technology for cultivating soybeans in acidic soil in tidal swamps. The objectives of this study were to measure nutrient uptake in the soybean plant using a saturated soil culture technique and to assess the impact of ameliorant addition in the form of rice biomass and rice hull on soybean yield. Two factors, rice biomass, and rice hull ash, were used in a split plot factorial design to set up the experiment. Four levels in the main factor included without biomass immersion, one immersion of short (3 cm above the root) rice stubble, one immersion of regular (panicle harvested only) rice straw, and two immersions of regular rice straw cut in the soil. The four levels in the subfactor were 0 t ha-1, 0.25 t ha-1, 0.50 t ha-1, and 0.75 t ha-1 of rice hull ash doses. Ameliorant in the form of biomass and rice husk increases soil quality in tidal swamps by enhancing pH, soil nutrients (N, P, K, S), CEC, cations, and base saturation, also reducing toxic compounds and improving soil texture. Application of ameliorant also increases soybean nutrient uptake and eventually increases the productivity of soybeans in tidal swamp acid soil. The best treatment of the first and second factors was one immersion of biomass with a regular cut of rice straw and 0.5 t ha-1 ash rice hull ash addition to the tidal swamp soil, respectively. 
Kebijakan Harga Kedelai dan Perlindungan Petani Ghulamahdi , Munif; Syarifuddin, Aip; Krisnamurthi, Bayu
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 3 No 1 (2021): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0301.167-170

Abstract

Terjadinya kelangkaan stok dan kenaikan harga kedelai saat ini merupakan kejadian berulang, dan ketergantungan Indonesia terhadap impor kedelai masih tinggi. Hal ini diperburuk dengan laju tren impor kedelai yang terus mengalami peningkatan selama 10 tahun terakhir (rata-rata 105 ribu ton/tahun). Produksi kedelai nasional saat ini adalah 940 ribu ton, sementara impor mencapai 2,75 juta ton. Adanya kelangkaan kedelai, seharusnya menyadarkan kita untuk tidak hanya mengandalkan instrumen perdagangan, namun perlu penguatan sistem produksi di dalam negeri. Untuk itu, diperlukan upaya peningkatan produktivitas dan penyediaan benih unggul kedelai melalui teknologi BJA (Budidaya Air Jenuh) di lahan pasang surut, serta adanya tata kelola kawasan produksi BJA, dengan tetap menjamin tersedianya benih unggul dan sarana produksi lainya. Tidak hanya itu, teknologi BJA perlu didukung dengan menjamin tersedianya produk turunan/produk akhir kedelai yang sesuai dengan kebutuhan pasar, sehingga daya saing kedelai lokal meningkat. Kemudian, sistem logistik yang memadai serta perlindungan petani sebagai bagian dari subsistem produksi kedelai juga sangat diperlukan. Pemerintah perlu mengatur sistem tata niaga kedelai yang setara dan berkelanjutan, serta memastikan kebijakan harga yang berpihak pada petani dan pengolah, seperti pengrajin Tahu Tempe. Pemerintah juga perlu menjamin pasokan kedelai berkualitas yang baik sesuai dengan kebutuhan nasional, dengan memperhatikan proporsi kedelai impor dan lokal.
PERTUMBUHAN DAN PRODUKTIVITAS KEDELAI DENGAN DOSIS DAN FREKUENSI PEMUPUKAN FOSFAT PADA BUDIDAYA JENUH AIR DI LAHAN PASANG SURUT Farizi, Ahmad Rifqi; M.S. , Prof. Dr. Ir. Munif Ghulamahdi,
Scientica: Jurnal Ilmiah Sains dan Teknologi Vol. 2 No. 9 (2024): Scientica: Jurnal Ilmiah Sains dan Teknologi
Publisher : Komunitas Menulis dan Meneliti (Kolibi)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.572349/scientica.v2i9.2360

Abstract

Konsumsi kedelai di Indonesia tahun 2020 mencapai 2,87 juta ton, sedangkan produksi nasional hanya 613.300 ton karena luas lahan kedelai menurun akibat persaingan komoditas dan perubahan lahan pertanian. Lahan pasang surut menjadi alternatif peningkatan areal pertanian menggunakan teknik budidaya jenuh air. Penelitian bertujuan mempelajari dosis dan frekuensi pemupukan P terhadap pertumbuhan dan produktivitas kedelai dengan budidaya jenuh air di lahan pasang surut. Penelitian menggunakan rancangan kelompok lengkap teracak faktorial dengan dua faktor dan tiga ulangan. Faktor pertama adalah dosis pupuk P terdiri atas: 36, 72, dan 108 kg P2O5 ha-1 (180, 360, dan 540 kg Fertiphos ha-1). Faktor kedua adalah frekuensi pemupukan P terdiri atas; pada saat tanam 0 hari setelah tanam (HST), 0 dan 30 HST, dan 0, 30, 60 HST. Perlakuan berbagai dosis pemupukan fosfat berpengaruh nyata terhadap peningkatan tinggi tanaman, jumlah cabang, jumlah daun, luas daun, bobot basah batang, bobot kering batang, bobot kering daun, jumlah polong isi, jumlah polong hampa, bobot biji ubinan, dan produktivitas. Perlakuan berbagai frekuensi pemupukan fosfat berpengaruh nyata terhadap peningkatan tinggi tanaman, jumlah cabang, jumlah bintil akar, jumlah polong isi, dan jumlah polong hampa. Dosis pupuk 108 kg P2O5 dan frekuensi pemupukan fosfat dengan tiga kali pemupkan pada 0, 30, dan 60 HST menjadi kombinasi terbaik untuk peningkatan produksi kedelai. Dosis pupuk 108 kg P2O5 ha-1 menghasilkan produksi kedelai tertinggi sebesar 2,60 ton ha-1.
Co-Authors , Hariyadi , Khaerana , Nirwan - Nuraeni -, Bachtiar . Hamim . Miftahudin A. HAITAMI Achmad Yozar Perkasa, Achmad Yozar AHMAD JUNAEDI Ahmad Rifqi Fauzi Aidi Noor Alce Ilona Noyaa Anas Dinurrohman Susila Andria Afrida Ani Kurniawati Ani Kurniawati Arraudah, Rahayu Atang Sutandi Atmaja, ian Surya Fitra Aziz, Sandra Bachtiar Bachtiar Bambang S. Purwoko Bambang Sapta Purwoko Bayu Krisnamurthi Bayuanggara Cahya Ramadhan Budi Hartoyo BUDI HARTOYO, BUDI BUDI MARTONO Cahyo, Septian Dwi Danner Sagala Desta Wirnas Didy Sopandie Dwi Guntoro Edi Djauhari Purwakusumah Edi Santosa Eko Sulistyono Endriani , Etty Pratiwi, Etty Faadhilah, Fajar Faqih Udin Farizi, Ahmad Rifqi Firdaus Puja Santana Fred Rumawas HAJRIAL ASWIDINNOOR Hamim Hamim Hamim Hamim Hanessa Putry, Regata Ringga Hardjosoewignyo, Soedarmadi Haridjaja, dan Oteng Haridjaja, Oteng Herawati, Nani Hermanasari, Rini Hermanto Hermanto HESTI PUJIwATI Hesti Pujiwati Hesti Pujiwati Hidayah , Riska Firotul Hizbi, Muhammad Syamil Homma, and Koki Ilona Noyaa, Alce Impron Impron Ince Raden Irdika Mansur Irdika Mansur Iskandar Lubis JAJAH KOSWARA Joedojono Wiroatmodjo Khairil Anwar Koki Homma LA KOLAKA LA KOLAKA, LA Latifah K. Darusman LATIFAH K. DARUSMAN M A Chozin Manullang, Frisca Anggriani Manullang, Nadya Inri MARIA BINTANG Marlin Sefrila Maulana, Arlingga Ichwan Maya Melati Miftahuddin Miftahuddin Miftahuddin Miftahudin . MIFTAHUDIN ., MIFTAHUDIN Muhammad Syamil Hizbi Muis, Ridwan Mustika Tripatmasari Tripatmasari Nasution, Siti Nurminah Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Nurwita Dewi Octivia Trisilawati Oteng Haridjaja Pienyani Rosawanti Purwono Purwono Purwono Purwono Purwono, Purwono Purwono, Purwono Ramadhan, Bayuanggara Cahya Rudi Suryadi Rudi Suryadi Sahuri Sahuri, Sahuri Salsabila Sandra A. Aziz Sandra Arifin Aziz Sandra Aziz Sari, Siti Hapita Sefrila, Marlin Selvie Diana Anis Sherly Rahayu Shiraiwa, Tatsuhiko Sintho Wahyuning Ardie Siti Maryam Harahap Siti Maryam Harahap Siti Ria Chaerunisa Sri Astuti Rais Sudirman Yahya Sudirman Yahya Sudradjat , Sugiyanta Sulistyono, dan Eko Suntari Suwarno Suwarno Suwarno, Suwarno Suwarto Suwarto Syarifuddin, Aip Taopik Ridwan Tatsuhiko Shiraiwa Taufan Hidayat Toyip Toyip TRI ASMIRA DAMAYANTI TRI HASTINI TRI KOESOEMANINGTYAS Trikoesoemaningtyas Turhadi Turhadi Ubad Badrudin Untung Susanto Wage Ratna Rohaeni Wahju Qamara Mugnisyah Wahyuni Lestari Willy Bayuardi Suwarno Yartiwi, Yartiwi Yonny Koesmaryono Yudhistira Nugraha Yudi Sastro YULIN LESTARI Yunita Fauziah Rahim Yunita Fauziah Rahim