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All Journal Jurnal Teknik ITS IPTEK Journal of Proceedings Series IPTEK The Journal for Technology and Science Jurnal Teknik Kimia Journal of Engineering and Technological Sciences Indonesian Journal of Biotechnology Indonesian Journal of Chemistry Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Teknik Kimia Indonesia Halal Research Journal Envirotek : Jurnal Ilmiah Teknik Lingkungan Sewagati Journal of Fundamentals and Applications of Chemical Engineering
Setiyo Gunawan
Departemen Teknik Kimia Institut Teknologi Sepuluh Nopember
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Comparative Study of DNA Extraction Methods for Halal Food Testing: Advantages, Disadvantages, and Recommended Methods Sari, Citra Yulia; Pamungkasa, Raditya Yudhi; Gunawan, Setiyo
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1734

Abstract

Identification of the halal status of food is a crucial aspect of the food industry, particularly in countries with a majority Muslim population. DNA extraction methods play a critical role in ensuring the halal status of food products by accurately detecting nonhalal substances. This study conducted a comparative evaluation of various DNA extraction methods used in halal food testing. We reviewed several DNA extraction methods listed in RSNI3 ISO 21571:2005 titled "Analytical Methods for the Detection of Genetically Engineered Products and Their Derivatives—Nucleic Acid Extraction". In RSNI3 ISO 21571:2005, five DNA extraction methods are discussed: the phenol-chloroform method, the silica method, the CTAB method, the polyvinylpyrrolidone (PVP) method, and the guanidine chloroform method. This study analyzes the advantages and disadvantages of the literature review. Additionally, we conducted an economic and process effectiveness review to identify the best method. Based on this analysis, we recommend the most suitable method for testing the halal status of various types of food sample. The results of this study are expected to serve as a guideline for testing laboratories and the food industry in selecting the optimal DNA extraction method for ensuring efficient and accurate halal status of products.
Lysis Buffers for Detecting Pork Content in Food Products Khoiriah, Ulfah Lailatul; Baydillah, Pramudja; Darmawan, Raden; Nasori, Nasori; Taufany, Fadlilatul; Gunawan, Setiyo
Indonesian Journal of Chemistry Vol 25, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.101620

Abstract

The increasing number of counterfeit processed food products with non-halal ingredients, such as pork, has caused public concern about the halalness of Indonesian food products, especially in the Muslim community. This issue has prompted the development of halal authentication techniques, including deoxyribonucleic acid (DNA) isolation followed by polymerase chain reaction (PCR) amplification to detect pork DNA. The main purpose of this research was to modify the lysis buffer using tris-ethylenediaminetetraacetic acid, cetyl trimethylammonium bromide, salt, alkaline, and urea. Two DNA extraction methods were evaluated using kit-based and conventional methods with modified lysis buffer. Using a fluorometer, it was proven that the conventional method with modified urea lysis buffer produced quite competitive DNA concentrations (119 ng for pork meat and 100.05 ng for pork sausage), as confirmed by real-time PCR analysis. As a result, the modified urea lysis buffer can be further developed because it is more affordable.
BNC production of siwalan neer and sugarcane molasses as novel media sources Hapsari, Dara Puspita; Perdani, Claudia Gadizza; Fahmi, Fahmi; Nurika, Irnia; Gunawan, Setiyo
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 7, No 2 (2024)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2024.007.02.3

Abstract

Bacterial nanocellulose (BNC) is a unique biopolymer with remarkable purity and material properties that distinguish it from plant-derived cellulose by its absence of lignin and hemicellulose. Recent research has focused on using organic waste materials as alternative substrates to reduce the cost of BNC production. This study examines the efficacy of Siwalan neer and sugarcane molasses as novel media for cultivating Komagataeibacter xylinus by comparing their performance with that of Hestrin and Schramm (HS) medium. The BNC harvested from these alternative media was rigorously characterized using scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to evaluate its structural and thermal properties. Notably, the use of Siwalan neer and sugarcane molasses as growth substrates enabled K. xylinus to produce BNC at a yield of 3.83 g/L, significantly exceeding the yield from the HS medium, which was 1.46 g/L. These findings highlight the potential of leveraging alternative substrates to enhance BNC production yield, cost-effectiveness, and environmental sustainability.
Evaluation of Polar Lipid Waste from Vegetable Oil Extraction: Insights from Experimental and Validated Simulation Results Husodo, Amani Salsabil; Indah, Nindia Noor; Wardani, Ratri Puspita; Gunawan, Setiyo
Envirotek : Jurnal Ilmiah Teknik Lingkungan Vol. 17 No. 2 (2025): Envirotek: Jurnal Ilmiah Teknik Lingkungan
Publisher : Universitas Pembangunan Nasional Veteran Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/envirotek.v17i2.2222

Abstract

This paper examined the wastewater production attributed to the solvent-based cooking oil extraction using Aspen Plus simulation facilitated which has been confirmed by experimental results. Distillate condensates that had remaining solvents as well as free fatty acids (FFAs) were identified as waste streams. Three feedstocks were tested, Crude Nyamplung Oil (CNO), high-FFA Crude Palm Oil (CPO-acid), and regular Crude Palm Oil (CPO-regular). CNO generated 0.288 t/h of hexane and methanol traces of wastewater, whereas CPO-acid (1.338 t/h) and CPO-regular (1.419 t/h) contained ethanol. The stoichiometric oxidation was used to estimate chemical oxygen demand (COD), whereas FFA content was used to determine oil and grease (O&G). The COD values of untreated streams were 293.236 mg/L (CNO), 2511.39 mg/L (CPO-acid), and 1662 mg/L (CPO-regular), which were higher than the Indonesian standard (350 mg/L). Oil and Grease (O&G) values of the untreated waste streams were not met the standards. Solvent based processes are less polluting as compared to refinery effluents (COD >15000 mg/L, O&G 4000mg/L), yet not in compliance with COD. Solvency and FFA value to biodiesel or PFAD are suggested to be enhanced to decrease the environmental burden.
Gerakan Pelatihan Ekspor Produk UMKM Jawa Timur Muntini, Melania Suweni; Rumiati, Agnes Tuti; Taufany, Fadlilatul; Cahyadini, Sarah; Armono, R Haryo Dwito; Gunawan, Setiyo; Rakhmawati, Nur Aini; Alawiyyah, Mujtahidatul
Sewagati Vol 10 No 1 (2026): Pre-Printed
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v10i1.7839

Abstract

Jawa Timur memiliki potensi besar dalam pengembangan Usaha Mikro, Kecil, dan Menengah (UMKM) untuk menembus pasar internasional, didukung oleh kekayaan sumber daya alam dan beragam produk unggulan di setiap kabupaten dan kota. Namun, UMKM menghadapi tantangan dalam hal daya saing, inovasi produk, dan akses pasar global. Pengabdian kepada Masyarakat ini bertujuan untuk meningkatkan daya saing UMKM Jawa Timur melalui pelatihan ekspor dan digitalisasi. Metode yang digunakan meliputi kolaborasi dengan Dinas Pemberdayaan Masyarakat dan Desa (DPMD), pengembangan kurikulum pelatihan, dan pelaksanaan workshop berbasis digital. Hasil menunjukkan bahwa pelatihan ini berhasil meningkatkan pemahaman pelaku UMKM terkait strategi ekspor, pemanfaatan teknologi digital, dan pengenalan pasar global. Selain itu, kegiatan ini secara langsung mendukung tujuan pembangunan berkelanjutan (SDGs) nomor 8 (pekerjaan layak dan pertumbuhan ekonomi), nomor 9 (industri, inovasi, dan infrastruktur), dan nomor 12 (konsumsi dan produksi yang bertanggung jawab). Respons positif dari peserta dan instansi terkait menunjukkan bahwa inisiatif ini berdampak signifikan dalam memotivasi UMKM untuk bersaing di pasar internasional. Pentingnya digitalisasi dalam memperluas jangkauan pasar serta keberlanjutan program ini menjadi langkah strategis untuk mendukung pertumbuhan ekonomi Jawa Timur.
Co-Authors Abdul Karim Amarullah Adesya Abdullah Agnes Tuti Rumiati Agus Rubiyanto Ahmad Muklason Aini Rakhmawati Aisyah Alifatul Zahidah Rohmah Alawiyyah, Mujtahidatul Ali Mufid Amani Salsabil Husodo Amelinda Angelina Aminuyati Amirotus S, Farah Amirut Tahfifah Anggita Claratika Aniendia Candra Suminta Anil Kumar Anal Annas Wiguno Annas Wiguno, Annas Anugraha, Rendra Panca Anwar, Hazrul Aparamarta, Hakun Wirasasista Aparamarta, Hakun Wirawasista Aparamarta, Hakun Wirawasista Ardy Maulidy Navastara Ari Prabowo Ari Prabowo, Ari Armono, R Haryo Dwito Ary Yusen Pratama Asalil Mustain Asalil Mustain Assidiqie, Gloria Islamy Atika Tri Antari Awaludin Rauf Firmansyah Awaludin Rauf Firmansyah Bambang Srijanto Baydillah, Pramudja Bayu Biru Chandra Brilian Prima Anindita Budi, Endah Cahyadini, Sarah Christa Kathleen Sitania Claudia Gadizza Perdani, Claudia Gadizza Claudya Patricia Caesy Darmawan, Raden Darmawan, Raden Debra Arlin Puspasari Della Istianingsih Desy Anggraini Dian, Nurul Huda Fauzan Naufal Doniarta Kurniawan Mintoko Eskalalita, Eskalalita Eunike Rhiza Febriana Setyadi Fabroyir, Hadziq Fadlilatul Taufany Fahmi Fahmi Fahmi, Fahmi Fahmi, Mohammad Zakki Fajrin, Alifah Nur Aini Faza Aruni Felycia Edi Soetaredjo Filan Setiawan Francis Sjarifudin Gadizza, Claudia Gede Wibawa Gede Wibawa Gunarto, Chintya Gunawan Gunawan Hadryan Ivander Hakun Wirawasista Hakun Wirawasista Hakun Wirawasista Aparamarta Hanny F. Sangian Hapsari, Dara Puspita Hartono, Sandy Budi Hasan Ikhwani Hellen Kartika Dewi Hilda Dwi Lestari Husodo, Amani Salsabil Ilham Muttaqin Zarkasie Indah, Nindia Noor Indraswari, Rarasmaya Indriana Purwaningsih Indriana Purwaningsih Ira Dwitasari Ira Dwitasari Irnia Nurika Ismadji, Suryadi Istianah, Nur Ita Ulfin Jeffry Tandrianto Junaidi Kristian Junaidy Kristian Junaidy Kristian Juwari Juwari Juwari Juwari Khoiriah, Ulfah Lailatul Kuswandi, Kuswandi Lailatul Qadariyah Lalitya Syifalia Lie, Jenni Mahardika, Gading Bagus Mahmudah , Liayati Maifiah, Mohd Hafidz Mahamad Majidah, Lailiyah Mashuri Mashuri Mawarni, Ruli Retno Mochammad Ainun Hikam Mochammad Ainun Hikam Mohammad Fandy Ardhilla Mohammad Fandy Ardhilla Muthmainnah, Melania Suweni Muntini Muzakhar, Syah Sultan Ali Muzakhar, Syah Sultan Ali Nahara, Anisa Ridha Nasori, Nasori Neza Anizar Noviyanto Noviyanto, Noviyanto Nur Aini Rakhmawati Nur Istianah Nurul Jadid Nurul Rahmawati Pambudi, Alfian Wisnu Pamungkasa, Raditya Yudhi Penny Diana Puspitawaty Perdani, Claudia Prasiefa, Mizanurafi’ Ghifarhadi Puspitasari, Nathania Puspitasari, Nurrisma Puspitawaty, Penny Diana Putri, Malfa Liya Putro, Jindrayani Nyoo Raden Darmawan Raden Darmawan Raden Darmawan Rahadiantino, Lienggar Rahmatunnisa Nur Salikha Rendra Panca Anugraha Rima Nur Febriani Risanti Delphia Rona Bening Larasati Safitri, Amelia Saifuddin Saifuddin Santoso, Shella Permatasari Sari, Citra Yulia Setyabudi, Latif Shade Rahmawati Silvya Yusnica Agnesty Silvya Yusnica Agnesty Silvya Yusnica Agnesty Silvya Yusnica Agnesty Siregar, Albar Sutan Bahari Sjarief Widjaja Sukma Rahma Sukma Rahma Sukmawati Rahma Sumarno . Sumarno Sumarno Supriono, Bambang Suryadi Ismadji Suyatno Suyatno Teguh Saputra Theresia Rosiana Tri Widjaja Uli A, Veny Wardani, Ratri Puspita Wenny Irawaty Wijaya, Christian Wijaya, Christian Julius William Ekaputra Taifan Wuwuh Wijang Prihandini Yi Hsu Ju Yoga Widhia Pradhana Yuliana Setyowati Yuliana, Maria Yusmiati, Siti Nur Husnul Yustianingsih, Lidia Zikrina Istighfarah Zuhdan, Muhammad J.