Heri Setiyo Bekti
Department Of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Denpasar, Denpasar, Bali, Indonesia And Center Of Excelence In Science And Technology, Polytechnic Of Health Denpasar, Bali, Indonesia

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Journal : MEDICA (International Medical Scientific Journal)

The Relationship Between Hepatitis B Surface Antibody Levels and the Levels of Serum Glutamic Pyruvic Transaminase, Serum Glutamic Oxaloacetic Transaminase, and Total Bilirubin Dewi, Ni Putu Ditya Prabandari; Bekti, Heri Setiyo; Purna, I Nyoman
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.492

Abstract

Hepatitis B virus is a disease that can impair other functions of the liver. This study aims to determine the relationship between Hepatitis B antibody levels and the levels of SGOT (Serum Glutamic Oxaloacetic Transaminase), SGPT (Serum Glutamic Pyruvic Transaminase), and bilirubin. A quantitative analytic study with a cross-sectional approach was conducted. Data collection involved primary data in the form of hepatitis B serum samples and secondary data including SGOT levels, SGPT levels, bilirubin levels, age, gender, and medical history. The results showed a significant and inverse relationship between Hepatitis B antibody levels and SGOT levels, with a Sig. (2-tailed) value of 0.029 (<0.05) and a negative correlation coefficient of -0.627. Similarly, there was a significant and inverse relationship between Hepatitis B antibody levels and SGPT levels, with a Sig. (2-tailed) value of 0.003 (<0.05) and a negative correlation coefficient of -0.616. However, there was no significant relationship between Hepatitis B antibody levels and bilirubin levels, as indicated by a Sig. (2-tailed) value of 0.809 (>0.05), although the correlation was positive (correlation coefficient = 0.078). In conclusion, Hepatitis B antibody levels show a significant negative correlation with SGOT and SGPT levels, while the correlation with bilirubin levels is positive but not statistically significant.
Differences in Bleed Sedimentation Rate and Hematocrit Values in Chronic Kidney Failure Patients Examined Before and After Hemodialysis at Tabanan Regional Hospital Cahyana, I Made Dwipa; Bekti, Heri Setiyo; Sudarmanto, I Gede
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.501

Abstract

Chronic Kidney Disease (CKD) is a pathological condition that is irreversible and has various underlying causes. The diagnosis is based on structural or functional abnormalities of the kidneys, with the primary criterion being a glomerular filtration rate (GFR) of less than 60 mL/min/1.73 m² for more than three months. Common symptoms include fatigue, loss of appetite, and muscle cramps. Management of CKD consists of two stages: conservative treatment aimed at slowing disease progression and renal replacement therapy, such as dialysis or kidney transplantation. Erythrocyte Sedimentation Rate (ESR) and Hematocrit (Hct) tests are important for detecting decreased erythropoietin production, which impacts red blood cell formation. The objective of this study was to examine the differences in ESR and Hct values in patients with chronic kidney disease before and after undergoing hemodialysis. The study employed a pre-experimental design using a One-Group Pretest-Posttest approach, involving a single experimental group without a control group. Measurements were taken both before and after the intervention. The results from 10 CKD patients at Tabanan Regional Public Hospital indicated that all respondents (100%) had elevated ESR levels both before and after hemodialysis. In contrast, Hct values decreased following hemodialysis, which is likely due to reduced erythropoietin production associated with CKD, leading to anemia. In conclusion, no significant difference was observed in ESR values before and after hemodialysis. However, there was a notable difference in Hct values, which declined after the procedure.  
Impact of Sample Delay on Leukocyte and Platelet Parameters in Dengue Fever Patients: A Hematological Analysis Utami, Luh Mitha Ari; Bekti, Heri Setiyo; Dhyanaputri, I Gusti Ayu Sri
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.502

Abstract

Background: The diagnosis of Dengue Fever (DF) involves physical examination, laboratory tests, and confirmation primarily through thrombocytopenia and leukocyte abnormalities. Laboratory procedures play a crucial role; however, delays in complete blood count (CBC) analysis may influence hematological parameters, potentially affecting diagnostic accuracy. Objective: To determine the effect of 2-hour and 4-hour delays in CBC testing on leukocyte and platelet counts in patients with Dengue Fever. Methods: This experimental study used a posttest-only control group design with purposive sampling. Blood samples from DF patients were divided into three groups: immediate testing, 2-hour delay, and 4-hour delay. Data were analyzed using the Shapiro-Wilk test followed by Multivariate Analysis of Variance (MANOVA). Results: The mean leukocyte and platelet counts in the immediate testing group were 4.86×10³/μL and 61.55×10³/μL, respectively. In the 2-hour delay group, leukocyte counts slightly decreased to 4.79×10³/μL and platelet counts increased to 65.77×10³/μL. In the 4-hour delay group, leukocyte counts increased to 4.85×10³/μL while platelet counts decreased to 64×10³/μL. MANOVA results (Sig. = 1.000) indicated no significant differences among the groups. Conclusion: Delays of 2 and 4 hours in CBC testing did not significantly affect leukocyte and platelet counts in Dengue Fever patients. These findings suggest that short-term delays in sample analysis may not compromise hematological results in DF diagnosis.
Analysis of LT and ST Genes of Enterotoxigenic Escherichia coli Using Polymerase Chain Reaction Method in Lawar Plek from Ketewel Village, Gianyar Hardiyanta, I Made Yogi; Swastini, I Gusti Agung Ayu Putu; Bekti, Heri Setiyo
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.504

Abstract

Lawar plek is a traditional Balinese dish made from raw meat, which poses a potential risk of contamination by pathogenic bacteria, including Enterotoxigenic Escherichia coli (ETEC). This study aimed to identify the presence of ETEC in lawar plek samples from Ketewel Village, Gianyar. This research uses molecular methods. The identification was conducted in two stages: initial isolation of suspected bacteria on Eosin Methylene Blue Agar (EMBA), followed by molecular confirmation using Polymerase Chain Reaction (PCR). The study results show that in EMBA culture, 4 out of 12 samples (33.33%) showed colonies presumptively identified as E. coli. However, PCR analysis revealed that none of these isolates carried the LT (heat-labile toxin) or ST (heat-stable toxin) genes characteristic of ETEC. The conclusion shows that neither the LT (heat-labile toxin) nor the ST (heat-stable toxin) virulence genes of Enterotoxigenic Escherichia coli were found in any of the samples tested as suspected Escherichia coli colonies on EMBA.
Co-Authors Ade Dharmawan Agus Sri Lestari Amany, Hana Amirah ANDI UDIN SARANSI Anniwati, Leonita Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Aprilia Rakhmawati Arjani, Ida Ayu Made Sri Artawa, I Made Budi Arwati, Ketut Lilik Ayu Sukma Wedha Kristyanti Burhannuddin Burhanuddin, Burhannuddin Cahyana, I Made Dwipa Cok Dewi Widhya Hana Sundari Cok Dewi Widhya HS Cok Dewi Widhya HS Cokorda Dewi Widhya Hana Sundari Desak Gede Dwi Agustini Desak Made Lestari Dewi Dewa Ayu Niti Rahayu Putri Dewi, Kadek Indra Trisna Dewi, Ni Km Ayu Kusuma Dewi, Ni Nyoman Astika Dewi, Ni Putu Ditya Prabandari Dewi, Pande Putu Ayu Patria Dharmawaiswari, Anak Agung Ayu Ratih Dharmawati, I Gst Agung Ayu Dwija, Ida Bagus Nyoman Putra Farsida, Farsida Fusvita Merdekawati Ganjar Noviar Hardiyanta, I Made Yogi Haribaik, Anastaysia Annisa Hasfika, Essydhea Husna, Ismalia I Gusti Agung Ayu Dharmawati I Gusti Agung Ayu Dharmawati I Gusti Agung Dewi Sarihati I Gusti Agung Dewi Sarihati I Gusti Ayu Made Wulan Diantari I Gusti Ayu Sri Dhyanaputri I Gusti Ngurah Teja Pratama I Made Krishna Paramartha I Made Suarjana I Made Suarjana I Made Yogi Hardiyanta I Nyoman Jirna I Nyoman Purna I Wayan Karta I Wayan Karta Ida Ayu Krisna Dwipayanti Ida Ayu Sri Arjani Ida Bagus Oka Suyasa Ida Bagus Oka Suyasa Indriyani Indriyani Jannah Sofy Yanty Kadek Ika Surya Cahyani Kosasih, Audia Nizhma Nabila Kurniawan, Surya Bayu Luh Ade Wilan Krisna Luh Ade Wilankrisna Luh Putu Laksmi Mahastuti, Shri Ayu Purnami Mardhia Mardhia Marpaung, Nurasi Lidya E. Mastra , Nyoman Merdekawati, Fusvita Mochamad Choirul Hadi Ni Kadek Wahyu Diana Sari Ni Komang Erny Astiti Ni Komang Juniawati Ni Komang Omik Trianita Udiana Ni Komang Sri Narayanthi Ni Nyoman Astika Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Intan Maha Ayu Diyanti Ni Putu Lisa Anjani Ni Putu Yulia Rahma Dewi Ni Wayan Rika Kumara Dewi Noviar, Ganjar Nur Habibah Nur Habibah Nyoman Mastra Posmaningsih, Dewa Ayu Agustini Pradnya Yasa, Ni Putu Candra Dewi Prianggandanni, Made Ayu Vita Purwani Ruth, Grace Monica Putu Amrita Paramahita Putu Diah Wahyuni Rakhmawati, Aprilia Rike Syahniar Rinawati, Luh Putu Rindi, Oktavelendi Dhaneta Graha Rofi'ah, Lisa Khofidatur Ruspawan, I Dewa Made Savitri, Ni Putu Ayu Dani Sofi Yanty, Jannah Sudarmanto, I Gede Sudiadnyana, I Wayan Surya Bayu Kurniawan Suwarriana, I Nyoman Astra Suyasa, Ida Bagus Oka Swastini, I Gusti Agung Ayu Putu Tedja, I Gusti Ayu Wiradari Utami, Luh Mitha Ari Wikantari, Ni Made Ayu Kerti Wilankrisna, Luh Ade Yanty, Jannah Sofy