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Antioxidant Activity of Lactobacillus spp as for Development of Antioxidantive Probiotic Komang Ayu Nocianitri; I Nengah Sujaya; Yan Ramona
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 1 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Probiotics are live microorganisms which when administered in adequate amount confer health benefit to the host. Our previous studies revealed that Lactobacillsus spp isolated from infant feces potential to be developed as probiotics. Since the functionality of probiotic is strain specific, it is worth to screen the antioxidant activity of those lactobacilli, which will be developed as antioxidative probiotic. The aim of this study was to screen antioxidant activities of Lactobacillus spp isolated from infant feces. A numbers of 20 Lactobacillus spp strains isolated from infant feces were used in this study. The antioxidant activities was determined in in vitro studies by measuring the lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity. The result showed that inhibitory activity of lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity exerted by of Lactobacillus spp was 10.12 - 83.02%, 16,50-46,73%, and 3.94 - 44.52%, respectively. The Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 showed the high antioxidant activity compared to the others lactobacilli. The antioxidant activity of Lactobacillus sp FBB 60 were 61,20%, 46,37%, 31,54%  and the activity of Lactobacillus sp FBB 81 were 57,01%, 29,31% 44,52 for the lipid peroxidation inhibitory activity, hydroxyl radical scavenging activity and ferrous chelating activity, respectively. This finding implied that the Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 are promising strain to be developed as antioxidative probiotic.
Antioxidant Activity of Lactobacillus spp as for Development of Antioxidantive Probiotic Komang Ayu Nocianitri; I Nengah Sujaya; Yan Ramona
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 1 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Probiotics are live microorganisms which when administered in adequate amount confer health benefit to the host. Our previous studies revealed that Lactobacillsus spp isolated from infant feces potential to be developed as probiotics. Since the functionality of probiotic is strain specific, it is worth to screen the antioxidant activity of those lactobacilli, which will be developed as antioxidative probiotic. The aim of this study was to screen antioxidant activities of Lactobacillus spp isolated from infant feces. A numbers of 20 Lactobacillus spp strains isolated from infant feces were used in this study. The antioxidant activities was determined in in vitro studies by measuring the lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity. The result showed that inhibitory activity of lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity exerted by of Lactobacillus spp was 10.12 - 83.02%, 16,50-46,73%, and 3.94 - 44.52%, respectively. The Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 showed the high antioxidant activity compared to the others lactobacilli. The antioxidant activity of Lactobacillus sp FBB 60 were 61,20%, 46,37%, 31,54%  and the activity of Lactobacillus sp FBB 81 were 57,01%, 29,31% 44,52 for the lipid peroxidation inhibitory activity, hydroxyl radical scavenging activity and ferrous chelating activity, respectively. This finding implied that the Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 are promising strain to be developed as antioxidative probiotic.
Effectiveness of Sludge Microbial Consortia in the Bioremediation of Detergent-Containing Launderette Wastes Yan Ramona; Esti Arisetya Dewi; Yenni Ciawi; I Wayan Budiarsa Suyasa
Cassowary Vol 5 No 2 (2022): June
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v5.i2.162

Abstract

This project investigated the potential of microbial consortia obtained from several sources to reduce or eliminate detergent concentration in the wastes of launderette businesses found in the capital city of Bali. Before being used as active starters, the consortium of microbiota contained in the 3 waste sources was enriched in a medium containing (g/l); 1 glucose, 0.05 K2HPO4, 0.05 KH2PO4, 0.05 (NH4)2[Fe(SO4)2].6H2O, 0.01 MgSO4, 0.01 yeast extract, 1800 mL distilled water, and 200 mL of wastewater of launderette industries. An important parameter used to indicate microbial growth was the level of the volatile solid substrate (VSS) in the enrichment medium. The microbial culture that showed the highest VSS only was further investigated in the main experiments by observing its effectiveness in reducing detergent linear alkyl-benzenesulfonates (LAS) and the chemical oxygen demand (COD) of launderette wastes. In this project, microbial consortia developed from detergent-containing launderette wastes were the most active starter to decrease the COD and LAS detergent content following 7 days of exposure. This starter (microbial consortia developed from this launderette waste) showed the highest value of VSS when compared to those collected from other sources, and this was reached on day 4. In the main experiment, 85.5% and 91.9% reduction of LAS and the COD of the wastes, respectively, were observed following exposure of the launderette waste with this culture starter. This indicated that these microbial consortia have the possibility to be developed as a potential starter in larger scales of detergent bioremediation.
Analysis of Phenolic and Tannin Contents in the Methanol Extract of Sweet and Sour Star Fruit Plants (Averrhoa carambola L) Leaves Commonly Used as Raw Materials of Lawar (A Balinese Traditional Food) Ni Putu Adriani Astiti; Sang Ketut Sudirga; Yan Ramona
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.334 KB) | DOI: 10.24843/ATBES.2019.v03.i01.p02

Abstract

Lawar, a special Balinese food made of leaves of star fruit plant, is commonly served in any Balinese traditional events, such as temple festival or wedding parties. The leaves of sweet and sour star fruit plant are used to make this traditional food. In this research, phenolic and tannin content of leaves of these types of plant were analyzed with a view to investigate their potential as antioxidant source. Phenolic compound has been well known to have antioxidant activity by neutralizing free radicals and stabilizing singlet oxygen. This compound has capacity to reduce free radicals by chelating metal ions or inhibiting enzymatic system, such as cyclo-oxygenase, mono-oxygenase or xanthine oxidase that catalyze formation of free radicals. [4] reported that plants with high content of phenolic compounds tend to have high nutritious and antioxidant activities Tannin is a compound belongs to polyphenolic group. Such compound is abundantly found in any plants. This compound has capacity to bind proteins, alkaloid, and gelatin. In human body, this compound play a very complex role, such as chelating metal ions, precipitating proteins, and functioning as biological antioxidant. The results of this study showed that the phenol content of sour and sweet star fruit plants were 2830.99/100 mg GAE and 1959.77/100 mg GAE, respectively, while the tannin content of these two plants were 18810.43 mg/100 gr TAE and 11035.75 mg/100 gr TAE, respectively, indicating that the leaves of sour star frit plant had better antioxidant activity than the sweet one.
The Use of Fish Waste Based Organic Fertilizer to Improve the Growth of Balinese Red Rice (Oryza Sativa L Cv. Barak Cenana) I Dewa Gede Cahyadi Kusuma; Ni Luh Suriani; Yan Ramona
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 5 No. 2 (2021)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.026 KB) | DOI: 10.29165/ajarcde.v5i2.67

Abstract

In recent years, wastes have been reprocessed into useful products, such as organic fertilizer. The application of organic fertilizers in farming practices can produce agricultural products that are safe for human health and the environment. The objective of this study was to investigate the effect of liquid organic fertilizer made from fish waste on the growth of Bali red rice seedlings. A Complete Randomized Design (CRD) consisted of 8 treatments with 5 replications was applied in this experiment. These included immersing of rice seeds in fish waste fertilizer with concentrations of 0.5% = P1, 1% = P2, 1.5% = P3, 2% = P4, 2.5% = P5 and 3% = P6 with volume/volume ratio (v/v), prior to soaking. Rice seeds without immersion in this fertilizer (C0) and those immersed in PGPR bacterial suspension only (C1) served as nil control and control treatments, respectively. The results showed that the application of 2.5% fish waste fertilizer produced the highest results, following measurement of parameters, including the percentage of germination (88.18%), plant height (28.78 cm), and root length (20, 74 cm) on day 15 after sowing.
INTRODUKSI PENGOLAHAN YOGURT DRINK DARI SUSU KAMBING DAN SARI BUAH NANAS DI DESA SEPANG BUSUNG BIU BULELENG I.N. Sujaya; K.A. Nocianitri; N.N.D. Fatmawati; N.W.A. Utami; I.D.P.K. Pratiwi; Y. Ramona
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.627 KB) | DOI: 10.24843/BUM.2022.v21.i03.p02

Abstract

Goat milk is produced by Tunas Mekar Goat farmer grout located at Sepang Village, Busungbisu, Buleleng. This product is highly potent to improve the income of the local farmers. Nevertheless, the use of goat milk as raw material for foods products is still limited. This program aimed to find out possible utilization of goat milk produced by local farmers at Sepang Village for the production varies of food products. Goat odors are the main problem in goat milk-based food products especially goat milk set-yogurt. Thus, the production of drink yogurt with the addition of pineapple juice is a promising technology to overcome goat odors in goat milk yogurt. This program was undertaken through sequential steps such as knowledge building of the housewives (the target population in this program) followed by training in yogurt making, drink yogurt making as well as product packaging and labeling. All programs were performed well as indicated by the active involvement of the participants. Throughout this program, the goat milk farmers group and housewives are now capable to produce drink yogurt to improve the value-added of their local produce goat milk, which possibly increase their economic income. Keywords: Goat milk, pineapple, Sepang, yogurt drink
Efektifitas daun salam dalam mengurangi cemaran mikroba penyebab busuk telur itik Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Intisari Sains Medis Vol. 13 No. 2 (2022): (In Press : 1 August 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.767 KB) | DOI: 10.15562/ism.v13i2.1321

Abstract

Background: Pathogenic bacteria on eggshells or egg contents will be very dangerous for the health of humans who consume them. Chemicals should not be an option for preservatives because they will cause side effects. In this study, we explored the possibility of using bay leaf extract (Syzygium polyanthum (Wight) Walp) to control the growth of polluting bacteria in salted duck eggs so that the shelf life can be extended. The purpose of the study was to determine the potential of the active compounds contained in bay leaves in reducing the total plate count/microbial contamination in salted duck eggs. Methods: Extraction of bay leaf powder was carried out by the infusion method. The activity test of bay leaf decoction (ARDS) was carried out using the in vitro diffusion well method on Mueller Hinton media. Identification of active compounds was carried out using the GCMS technique. Results: ARDS crude extract at a concentration of 5% (v/v) could significantly reduce ALT and inhibit the in vitro growth of Proteus mirabilis bacteria at a concentration of 10% (w/v), with an inhibition zone diameter of 19.53 mm, with the minimum value inhibitory concentration (MIC) 0.7%. The results of the GCMS analysis of ARDS showed that the ten peaks produced, possibly synergized, and had antimicrobial activity. These compounds are 2-Pentanone4-Hydroxy-4 Methyl (CAS) Diacetone Alcohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester (CAS) Methyl Laurate; Tetradecanoic Acid, Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 and Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate Conclusion: Leaf decoction can inhibit the growth of Proteus mirabilis in vitro, and the addition of ARDS with a concentration of 5% (v/v) can significantly reduce the value of bacterial contamination in salted duck eggs.   Latar Belakang: Bakteri pathogen pada kulit telur atau isi telur akan sangat membahayakan kesehatan manusia yang mengkonsumsinya. Bahan kimia seharusnya tidak menjadi pilihan untuk pengawet, karena akan menimbulkan efek samping, Pada penelitian ini dieksplorasi, kemungkinan dipakainya ekstrak daun salam (Syzygium polyanthum (Wight) Walp) untuk mengontrol pertumbuhan bakteri pencemar pada telur itik yang diasinkan, sehingga masa simpannya dapat diperpanjang. Tujuan penelitian untuk mengetahui potensi senyawa aktif yang terdapat pada daun salam dalam menurunkan angka lempeng total/cemaran mikroba pada telur itik yang diasinkan. Metode: Ekstraksi serbuk daun salam dilakukan dengan metode infusa. Uji aktifitas air rebusan daun salam (ARDS) dilakukan dengan metode sumur difusi secara in vitro pada media Mueller Hinton. Identifikasi senyawa aktif dilakukan dengan teknik GCMS. Hasil: Ekstrak kasar ARDS pada konsentrasi 5% (v/v) secara nyata dapat menurunkan ALT dan menghambat pertumbuhan in vitro bakteri Proteus mirabilis pada konsentrasi 10% (b/v), dengan diameter zona hambat sebesar 19,53 mm, nilai minimum inhibitory concentration (MIC) 0,7%. Hasil analisis GCMS terhadap ARDS menunjukan bahwa sepuluh puncak yang dihasilkan, kemungkinan bersinergi dan mempunyai aktifitas sebagai antimikroba. Senyawa-senyawa tersebut adalah 2-Pentanone4-Hydroxi-4 Methyl (CAS) Diacetone Alkohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester(CAS) Methyl Laurate; Tetradecanoic Acid,Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 dan Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate. Simpulan: Air rebusan daun dapat menghambat pertumbuhan Proteus mirablis secara in vitro dan penambahan ARDS dengan konsentrasi 5% (v/v) secara signifikan dapat menurunkan nilai cemaran bakteri (angka lempeng total) pada telur itik yang diasinkan.
Kajian Pustaka: Penanggulangan Kandidiasis Menggunakan Pendekatan Probiotik Ni Made Teriyani; Fainmarinat Selviani Inabuy; Yan Ramona
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.854 KB) | DOI: 10.19087/jveteriner.2022.23.2.281

Abstract

Kandidiasis merupakan infeksi yang disebabkan oleh kamir kelompok Candida, terutama C. albicans. Kamir ini bersifat oportunis, karena C. albicans dapat menunjukkan karakateristik patogenitasnya atau berubah menjadi pathogen bila terjadi perubahan-perubahan kondisi lingkungan di tempat-tempat dimana species kamir ini berasosiasi dengan mikrob lain pada inangnya. Perubahan-perubahan kondisi lingkungan ini umumnya menyebabkan terjadinya perubahan komposisi mikrobiota pada bagian-bagian tersebut, dan perubahan ini dapat bersifat lokal atau sistemik pada inangnya. Dalam kondisi normal, pertumbuhan C. albicans dikendalikan/dikontrol oleh kehadiran mikrob lain dengan komposisi seimbang yang ada disekitarnya. Bila terjadi ketidakseimbangan mikrobiota normal pada tempat-tempat tersebut, maka kondisi ini akan dimanfaatkan oleh C. albicans untuk tumbuh dan berkembang melampaui mikroba lain. Pertumbuhan berlebih C. albicans ini sering menimbulkan masalah atau menyebabkan infeksi/lesi. Pada inangnya, kamir ini sering berasosiasi pada mukosa rongga mulut, permukaan kulit, saluran pencernaan, serta alat reproduksi wanita (vagina), dan menyebabkan infeksi yang disebut dengan kandidiasis. Kasus kandidiasis terus mengalami peningkatan dalam beberapa tahun terakhir, yang disebabkan oleh munculnya kasus resistansi Candida spp., terhadap antifungi yang dipakai dalam pengobatan kandidiasis. Pemahaman menyeluruh terkait kandidiasis menjadi sangat penting dan diperlukan untuk membangun strategi potensial dalam penanggulangan kandidiasis. Oleh karena itu, dalam telaah pustaka ini dielaborasi berbagai topik penting terkait kandidiasis dan alternatif penanganannya. Semua informasi yang diperlukan diekstrak dari berbagai artikel terkait yang dipublikasikan di jurnal-junal ilmiah bereputasi. Beberapa aspek seperti mekanisme Candida spp., dalam menyebabkan infeksi, faktor-faktor predisposisi, permasalahan dalam terapi kandidiasis menggunakan agen antifungi, maupun terapi alternative (termasuk pendekatan biokontrol menggunakan probiotik potensial) dijabarkan secara komprehensif dalam telaah pustaka ini
Penyediaan Air Bersih di Era Tatanan Normal Baru Yenni Ciawi; Ananda Sri Phartiswari; Yan Ramona; Annisa Maria Hidayati
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol. 13 No. 2 (2022)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pengairan.2022.013.02.07

Abstract

Tahun 2020 merupakan tahun terpanas sepanjang sejarah manusia, padahal sejak akhir 2019 kegiatan manusia yang menimbulkan polusi udara sudah berkurang banyak karena pandemi Covid-19. Pemanasan global yang sudah berlangsung menyebabkan cuaca ekstrim di banyak tempat di seluruh dunia. Sebagian permukaan bumi semakin kering sedangkan di bagian yang lain, banjir dan angin topan semakin sering terjadi. Sementara itu, pandemi Covid-19 yang mengharuskan cuci tangan lebih sering, menyebabkan kebutuhan air semakin meningkat dengan pesat. Tujuan tulisan ini adalah merangkum upaya-upaya penyediaan air yang sudah ada yang dapat dilakukan untuk meminimkan dampak kekeringan pada saat pandemi Covid-19 dan pada masa tatanan normal baru. Penelitian dilakukan dengan metode review traditional terhadap tulisan pada rentang tahun 1981-2022. Ditemukan bahwa kearifan lokal dan  teknologi baru yang relatif sederhana dapat dimanfaatkan untuk penyediaan air bersih bahkan sampai tataran rumah tangga sehingga menjangkau masyarakat banyak, yaitu dengan desalinasi air laut, memanfaatkan air hujan, menyaring air limbah, memanfaatkan waduk bawah tanah, menggunakan saringan batu tradisional, saringan keramik, dan membran polimer.
Potensi madu dan propolis lebah Tetragonula laeviceps dalam menghambat pertumbuhan in vitro bakteri Staphylococcus aureus dan Escherichia coli Fernando Putra; Yan Ramona; I Made Saka Wijaya
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p01

Abstract

Madu, yang sangat baik untuk kesehatan manusia karena kandungan gizinya yang tinggi, merupakan produk dari lebah Tetragonula laeviceps. Selain madu, lebah ini juga menghasilkan beberapa produk turunan, seperti propolis yang sering digunakan sebagai suplemen kesehatan. Madu dan propolis telah banyak dilaporkan memiliki aktivitas antimikroba. Oleh karena itu, tujuan utama penelitian ini adalah untuk mengetahui efektivitasnya dalam menghambat Staphylococcus aureus dan Escherichia coli pada berbagai uji in vitro (termasuk penentuan nilai MIC dan LC50). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 taraf konsentrasi (15%, 20%, 30%, 40%, 50%, 100%) untuk menentukan kedua produk lebah tersebut dalam menghambat pertumbuhan in vitro S. aureus dan E. coli. Etanol (etanol 95%) yang digunakan sebagai pelarut dalam percobaan 5 ulangan ini, berfungsi sebagai kontrol negatif. Zona hambat dan nilai MIC ditentukan dengan menggunakan metode sumur difusi, sedangkan metode pour plate pada media nutrien agar diterapkan dalam penentuan nilai LC50 madu dan propolis. Hasil penelitian menunjukkan bahwa madu konsentrasi 100% menghambat pertumbuhan S. aureus dan E. coli dengan zona hambat masing-masing 12,50 ± 1,09 mm dan 6,60 ± 0,60 mm. Hasil serupa juga ditunjukkan oleh propolis dengan diameter penghambatan 15,50 ± 1,08 mm dan 8,10 ± 1,00 mm terhadap S. aureus dan E. coli. Baik madu maupun propolis ternyata memiliki nilai MIC 15% (v/v), sedangkan nilai LC50 pada S. aureus masing-masing adalah 35,15% dan 18,25%, dan pada E. coli masing-masing adalah 35,89% dan 28,2%. Propolis memiliki daya hambat yang lebih kuat terhadap S.aureus dan E. coli dibandingkan madu.
Co-Authors A. Line, Martin A.A. Ketut Darmadi Agestiawan, I. G. A. M. Agung Panji Dwipayana, I Dewa Alayna Himawan Anak Agung Gede Indraningrat Anak Agung Ketut Darmadi Anak Agung Ngurah Nara-Kusuma Ananda Sri Phartiswari Andriani, Luh Nyoman Ary Metta Anissa Maria Hidayati Ayu Amelia Bagas Susilo Batho, Yanuarius Felix Cok. Istri Dewiyani Cakrawati Cokorda Istri Sri Arisanti Dandy Bimantara Dewa Ayu Andriastini Dewa Ayu Swastini Diah Kharismawati Djereng Esti Arisetya Dewi Fainmarinat Selviani Inabuy Fernando Putra Flavia Fionessa Falenda Shanzet Fukuda Kenji Gusti Ayu Dianti Violentina Gustu Widi Putra Hani, Ni Luh Helda Thorikul Affan I D.P. Kartika Pratiwi I Dewa Gede Cahyadi Kusuma I Dewa Made Sukrama I G. A. Widagda I Gusti Agung Gde Bayu Wirama I Gusti Ngurah Jemmy Anton Prasetya I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Oka Adi Parwata I Made Saka Wijaya I N.K. Widjaja I Nengah Sujaya I Putu Aditya Prayoga I Putu Oka Suartama I W BUDIARSA SUYASA I Wayan Suanda I Wayan Suardana I. M. A Sasmitha I.B. Oka Suyasa I.D.P.K. Pratiwi IBG Darmayasa Ida Ayu Ketut Ariningsih Ida Ayu Putu Suryanti Ida Ayu Sri Sinca Maha Uni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara Inabuy, Fainmarinat selviani Irma Damayanti K.R Suciptha Kadek Wegi Kurnilia KOMANG AYU NOCIANITRI Komang Dharmawan Kusuma, I Dewa Gede Cahyadi Meitini Proborini Wahyuni N. N. Rupiasi N.G.A Manik Ermayanti N.W.A. Utami Nadia Khairunnisa Nadya Treesna Wulansari Ni LUH ARPIWI Ni Luh Made Ema Juliasari Ni Luh Putu Ariwathi Ni Luh Suriani Ni Made Susilawati Ni Made Teriyani Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Rupiasih Ni Putu Adriani Astiti Ni Putu Desy Aryantini Ni Putu Eka Leliqia Ni Putu Indiani Sahaning Karna Ni Putu Linda Laksmiani Ni Putu Suariani Ni Putu Widarini Ni Putu Widiantari Ni Wayan Eka Putri Gayatri Kastawa Ni Wayan Nursini NYOMAN SEMADI ANTARA Pande Gde Sasmita Julyantoro Pande Putu Ayu Sukmawati Putu Eka Sudaryatma Putu Rima Sintyadewi Retno Kawuri SANG KETUT SUDIRGA Sentot Joko Raharjo Septiari, I Gusti Ayu Agung Shetty, Kalidas Sri Wahyuni Sumartin N.W.E. Tadashu Urashima Teriyani, Ni Made Viviandari, I D. A. A Wayan Redi Aryanta Wulandari, Kristina Yenni Ciawi Yoshitake Orikasa Yuji Oda Yulidia Iriani Yulidia Iriani Yustiantara, Putu Sanna Zulfa, Rozifatul