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All Journal HAYATI Journal of Biosciences Archive of Community Health METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari BIOTROPIA - The Southeast Asian Journal of Tropical Biology SIMBIOSIS Agrointek Jurnal Teknik Pengairan: Journal of Water Resources Engineering Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) ISM (Intisari Sains Medis) : Jurnal Kedokteran Advances in Tropical Biodiversity and Environmental Sciences Cassowary AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Acitya Wisesa: Journal of Multidisciplinary Research Jurnal Pengabdian Kepada Masyarakat Widya Mahadi Nata Palemahan: Journal of Environmental Engineering Innovations
Yan Ramona
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Social Sciences Veterinary

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YEAST PROBIOTICS WITH POTENTIAL TO ASSIMILATE CHOLESTEROL IN VITRO YAN RAMONA; NI LUH PUTU ARIWATHI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.3.1768

Abstract

 In the last two decades the use of yeasts as new probiotics has increased significantly. Therefore, our current research was focused on the investigation of yeasts for novel probiotic development in Bali. The main objectives of this research were to isolate and characterize yeasts isolated from ragi tape (dried mix cultures of microorganisms normally used in the fermentation of rice or cassava in Indonesia) and tape ketan (fermented sticky rice) for possible use as yeast-based novel probiotics, with capability to assimilate cholesterol in vitro. In this study, the potential yeast isolates were evaluated for survival at low pH conditions (pH 2, 3, or 4) and in high levels of sodium deoxicholic (NaDC), at concentrations of 0.2, 0.4, or 0.6 mM. In addition, the yeast isolates were also evaluated for their ability to assimilate cholesterol in vitro and to elucidate biotransformation of cholic acid into deoxycholic acid. This study led to 10 isolates that were resistant to pH levels of 2, 3, or 4 and to NaDC at concentration of higher than 0.4 mM. Most of those isolates were also found to assimilate cholesterol in vitro at the rate of between 18% and 76% in 24 hours incubation. In the biotransformation test, none of those isolates transformed cholic acid into deoxycholic acid, indicating that they are safe and have potential to be developed into novel probiotics, either for human or cattle
Pengaruh suplementasi buah dan lama fermentasi terhadap mutu organoleptik kombucha teh hitam Flavia Fionessa Falenda Shanzet; Ni Putu Indiani Sahaning Karna; Nadia Khairunnisa; Ayu Amelia; Alayna Himawan; Ida Bagus Gede Darmayasa; Yan Ramona
Cassowary Vol 6 No 1 (2023): Januari
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v6.i1.194

Abstract

ABSTRACT Kombucha is a beverage made from tea as its basic ingredient and fermented by active starter of microbial consortia. The flavor of this fermented beverage is determined by many factors, such as supplementation of the main raw ingredient with various fruits, sugar, and types of tea used as its main raw ingredient. The main objective of this research was to elucidate the effect of fruit supplementation (addition of Fuji apple and strawberry) on the organoleptic quality of the products, following incubation periods of 4, 8, and 12 days. A triplicate complete randomized factorial design (types of fruits combined with incubation period) was applied in the experiment. Organoleptic tests (include taste, aroma, and color of the products) were conducted by applying quisionere method involving 7 panelis. The results showed that types of fruits and incubation period significantly affected (p<0.05) the organoleptic quality of the kombucha products. Overall, combination of stroberry and incubation period of 4 days gave the best results (p<0.05) when compared to control (commercially sold kombucha) and other treatments, indicating that such product has potential to be widely commercialized. Keywords: Alcohol, fermented beverage, fermentation, kombucha, organic acids ABSTRAK Kombucha merupakan minuman hasil fermentasi yang terbuat dari bahan dasar teh setelah diinokulasi dengan mikroba starter. Cita rasa kombucha sangat ditentukan oleh penambahan suplemen, seperti gula, buah-buahan, dan jenis teh yang dipakai. Tujuan utama penelitian ini untuk mengelusidasi pengaruh penambahan jenis buah (buah apel dan buah strobery) dan lama waktu fermentasi (4, 8, dan 12 hari) terhadap kualitas organoleptik produk kombucha yang dihasilkan. Penelitian ini menggunakan rancangan acak lengkap factorial (jenis buah yang dikombinasi dengan lama fermentasi) dengan 3 kali ulangan untuk setiap kombinasi perlakuan. Uji organoleptik (rasa, warna, dan aroma) dilakukan dengan metoda kuesioner yang melibatkan 7 orang panelis. Hasil penelitian menunjukkan bahwa jenis buah dan lama fermentasi berpengaruh nyata terhadap tingkat kesukaan panelis pada produk kombucha. Secara keseluruhan, perlakuan kombinasi penambahan buah stroberi dan waktu fermentasi selama 4 hari memberikan hasil terbaik, dan berbeda nyata dengan kontrol maupun perlakuan lainnya. Hal ini mengindikasikan bahwa produk penelitian ini berpeluang untuk dipasarkan secara luas. Kata kunci: Alkohol, asam organik, fermentasi, kombucha, minuman terfermentasi
Production of Biogas As An Alternative Green Energy with Organic Wastes As The Main Raw Materials Yenni Ciawi; Ni Made Utami Dwipayanti; Anak Agung Gede Indraningrat; Yan Ramona
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.138

Abstract

This research focused on the utilization of four different organic wastes, namely snake fruit, orange, cabbage, and tomato wastes, for the production of biogas. The main objectives were twofold: (1) to investigate the characteristics and biodegradability of these wastes, and (2) to evaluate their potential for anaerobic methane production. The experiment was conducted using 250 L bioreactors, with the four wastes serving as the primary raw materials. A starter culture of cattle dung was added, and the mixture was incubated for eight weeks. Regular sampling and analysis were carried out to assess water content, biodegradability, specific rate of volatile material reduction, and gas yield. The results showed that the water content of the four waste systems remained relatively consistent throughout the experiment. Biodegradability analysis revealed that all of the wastes were biodegradable, with varying levels of degradation ranging from 23.10 ± 2.89% to 59.84 ± 4.17%. Snake fruit waste exhibited the highest resistance to degradation, while tomato waste was the most easily degradable. Kinetic analysis indicated specific rates of volatile material reduction (µ) of 0.006 ± 0.0006 per day for the most resistant waste and 0.0170 ± 0.0021 per day for the least resistant waste. The incorporation of these four waste types in the biogas production process had a positive effect on gas formation. Therefore, these organic wastes hold promise as valuable resources for biogas production, addressing both the issue of waste accumulation and the energy crisis in an environmentally beneficial manner.
Role of Catecholamine Hormone to Increase the Virulence of Aeromonas hydrophila Ni Putu Widiantari; Yan Ramona; Pande Gde Sasmita Julyantoro
Metamorfosa: Journal of Biological Sciences Vol 10 No 2 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i02.p17

Abstract

Lingkungan yang ekstrim telah banyak dilaporkan dapat memicu terjadinya stres pada mahluk hidup yang terpapar, sehingga mengganggu kondisi homeostasinya. Dalam keadaan tertekan, organisme vertebrata akan melepas hormon-hormon stres, seperti katekolamin Norepinephrin (NE) dan Dopamin (Dopa), yang melibatkan axis hypothalamus-sympathetic-chromaffin (HSC) dan axis hypothalamus–pituitary-interrenal (HPI). Kedua sistem axis tersebut berturut-turut menghasilkan hormone katekolamin (antara lain adrenalin, noradrenalin, dan dopamine) dan hormon kortikosteroid (terutama kortisol). Kehadiran kedua kelompok hormon tersebut pada sistem peredaran darah inang berperan sebagai signal bagi bakteri patogen, seperti Aeromonas hydrophila, yang umum ditemukan dalam akuakultur, untuk memulai proses infeksinya. Hormon berperan untuk mengaktifkan gen-gen dalam aktivitas dan pembentukan flagella (memungkinkan sel bakteri berenang dengan cepat menuju inangnya), pembentukan pilli, produksi toksin, dan mengaktivasi proses pembentukan biofilm. Dengan kata lain, kondisi stres pada inang ini mempengaruhi metabolisme, pertumbuhan dan virulensi bakteri patogen tersebut, karena kondisi sistem imun inang berada pada titik terendah ketika organisme tersebut mengalami stres. Sekresi hormone katekolamin juga merupakan signal bagi bakteri patogen untuk mengaktifkan gen ferric uptake regulator (fur gene) yang berperan dalam proses pengkhelatan zat besi (Fe3+) di lingkunganya, sehingga terjadi pertumbuhan berlebih dari patogen tersebut. Peran hormone katekolamin dalam peningkatan patogenitas patogen adalah melemahkan ikatan kompleks protein transferrin dengan zat besi (Fe3+), sehingga terjadi peningkatan laju pertumbuhan patogen yang akhirnya berdampak pada peningkatan patogenitasnya. Kata kunci : Akuakultur, Endokrinologi mikroba, Patogenisitas, Stres.
Kajian Pustaka: Bakteri Asam Laktat Halotoleran: Prospek Pengembangan Metoda Baru Untuk Menekan Pembentukan Histamin pada Hasil Laut Ramona, Yan; Andriani, Luh Nyoman Ary Metta; Sukmaningsih, Anak Agung Sagung Alit
Bumi Lestari Journal of Environment Vol 24 No 2 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Abstract Histamine is an amine biogenic compound, frequently reported to cause poisoning among people who consume fishes with high level of histamine. Such compound is produced by histamine producing bacteria (HPB) belong to family of Enterobacteriaceae. Histidine decarboxylase produced by HPB plays a central role to convert free histidine in the fish meat into histamine, resulting in accumulation of histamine in the fish meat. Many efforts, including low temperature storage, ozone, and brine treatment) have been applied to cope with/prevent histamine accumulation in seafood products. However, those methods have some disadvantages, such as costly or have potential to change texture or structure of the products being preserved. In this review therefore, some aspects (including application of lactic acid bacteria (LAB) to control histamine production, characteristics of LAB required, tolerance of LAB to high level of salt, and the role of LAB to suppress HPB growth, and suppression of histamine production) are comprehensively reviewed. All information was extracted from related scientific articles published in the last decade. Keywords: Allergy; Biogenic agent; Histidine decarboxylase; Lactic acid bacteria; Tuna
SOSIALISASI DAN PELATIHAN PENANAMAN MANGROVE DI KAMPOENG KEPITING KELURAHAN TUBAN, KECAMATAN KUTA, KABUPATEN BADUNG-BALI I Wayan Suanda; IBG Darmayasa; A.A. Ketut Darmadi; Yan Ramona
Jurnal Pengabdian Kepada Masyarakat Widya Mahadi Vol. 4 No. 2 (2024): Juni 2024
Publisher : LP3M Universitas PGRI Mahadewa Indonesia

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Abstract

Tuban Village, Kuta District, Badung Regency has quite a wide coastline and is the estuary of several rivers in Badung Regency. Geologically, the beach consists of alluvium deposits composed of pebbles, gravel, sand, silt and clay resulting from river water and sea water sedimentation processes, which are very good for the growth of mangroves. Mangroves are not only a collection of plants, but are one of the natural resources that can provide benefits to the human life and the lives of the marine biota. This condition did not last long due to rapid development causing the conversion of coastal land for fisheries cultivation, settlements and industry which had an adverse impact on the mangrove ecosystem. The death of mangroves and depletion of living habitat resulted in a decreasing mangrove population. The existence and sustainable of the mangrove forests need to be maintained through local community involvement in the form of Kampoeng Kepiting ecotourism to gain economic value. The mangrove forest rehabilitation program takes the form of planting and maintenance as one of the efforts to restore the mangrove ecosystem in Kampoeng Kepiting, Tuban Village, Kuta District.
THE EFFECTS OF LACTIC ACID BACTERIA GROWTH INHIBITORS OF STAPHYLOCOCCUS EPIDERMIDIS SUPPLEMENTATION ON THE QUALITY OF ANTI-MICROBIAL LOTION Zulfa, Rozifatul; Ramona, Yan; Arpiwi, Ni Luh
Acitya Wisesa: Journal of Multidisciplinary Research Vol. 3 Issue 1 (2024)
Publisher : jfpublisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56943/jmr.v3i1.581

Abstract

Staphylococcus epidermidis infections of the skin can cause boils, itching and long-lasting sores. Currently, around 75% to 90% of S. epidermidis infection cases are resistant to the antibiotic methicillin. Thus, this becomes a concern in the health sector. This research aims to discover a new method to treat S. epidermidis infection by using lactic acid bacteria as its opponent, so that the use of antibiotics can be reduced. The physical test includes confirmation of Lactic Acid Bacteria (LAB), inhibition test of lab on S. epidermidis, lotion formulation, organoleptic and homogeneity test of lotion, lotion hedonic test, LAB total plate count test in lotion, and data analysis using Microsoft Excel, Statistical Program Service Solution (SPSS), and ANOVA test. Lactic acid bacteria isolates were obtained from previous research stocks and tested for their ability to inhibit S. epidermidis in vitro. These lactic acid bacteria were added to the lotion. The favorability level of consumers to the lotion was also tested. The results indicated that lactic acid bacteria were able to inhibit the growth of S. epidermidis and remained effective until the seventh day. In addition, respondents provided a positive response to the physical quality of the lotion that contained lactic acid bacteria.
Viabilitas probiotik Lacticaseibacillus rhamnosus SKG34 pada sari buah terung belanda (Solanum betaceum Cav.) terfermentasi selama penyimpanan pada suhu dingin Nocianitri, Komang Ayu; Pratiwi, I Desak Putu Kartika; Wulandari, Kristina; Ramona, Yan; Sujaya, I Nengah
AGROINTEK Vol 19, No 3 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i3.26535

Abstract

Tamarillo (Solanum betaceum Cav.) is a tropical fruit that has the potential to be developed into functional food in the form of fruit juice containing probiotics. In order to develop the probiotic-containing product, the suitability of fruit juice to support the growth of probiotics and the viability of probiotics during the fermentation and storage processes are the prime concerns. Therefore, this study aims to determine the viability of the local probiotic Lacticaseibacillus rhamnosus SKG34 and the characteristics of fermented Tamarillo juice during storage at 4oC. Fermented tamarillo juice containing L. rhamnosus SKG34 is produced by fermenting Tamarillo juice with L. rhamnosus SKG34 and then stored at a temperature of ±4oC for 30 days. Population of L. rhamnosus SKG34, total sugar, total acid, pH, vitamin C, antioxidant capacity, and sensory characteristics were determined at 0, 5, 10, 15, 20, 25, and 30 days. The results showed that the probiotic L. rhamnosus SKG34 in fermented tamarillo juice was able to survive for 30 days with a population of 2.58 x 1010 CFU/ml from the initial population of 9.54 x 1010 CFU/ml. However, considering that chemical characteristics and other functional properties also influence the acceptability of fermented tamarillo juice, the optimal storage time is 20 days at 4oC. Under this condition, the fermented tamarillo juice containing 3.6 x 1010 CFU/ ml of L. rhamnosus SKG34; 11.23% sugars; total acid 0.98% acids; pH 3.44; 31.76 mg/100 ml vitamin C; and 4,725 GAEC/100 ml of antioxidant capacity, with favorable sensory levels.
THE THE EFFICACY OF BACTERIAL AND FUNGAL ANTAGONIST SUSPENSIONS IN CONTROLLING FOLIAR MILDEW DISEASE IN ZUCCHINI PLANTS: - Ramona, Yan; A. Line, Martin; Septiari, I Gusti Ayu Agung; Darmayasa, Ida Bagus Gede; Agung Panji Dwipayana, I Dewa; Shetty, Kalidas
BIOTROPIA Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.2.2448

Abstract

ARTICLE HIGLIGHTS- Diverse microbial antagonists can be used as alternatives to control foliar disease - Microbial agents offer zucchini protection against downy mildew causing microbes- Biocontrol agents effectively control mildew infection in zucchini plants- Diverse microbial antagonists have potential to control foliar disease in zucchini - New bio-based strategy supports sustainable crop disease management- Diverse microbial antagonists are promising for controlling mildew in zucchiniABSTRACTDowny mildew is recognized as a major constraint in zucchini production, caused by obligate fungal-like pathogens that thrive under humid conditions. In this study, the efficacy of selected bacterial (Lysobacter antibioticus Bali G, Pseudomonas corrugata SAJ6) and fungal (Trichoderma sp. Td22) antagonists was evaluated for the management of this foliar disease on zucchini plants as an alternative to chemical fungicides. The efficacy of these bacterial and fungal antagonists against a suspected downy mildew pathogen was assessed on zucchini leaves in a glasshouse. It was found that the antagonists provided 22 - 83% protection (P < 0.05) against the pathogen two weeks after application. However, the level of protection declined over time, with 46 - 60% of leaves infected five weeks after pathogen exposure, regardless of treatment. The combination of Trichoderma sp. Td22, the most effective agent, with either Lysobacter antibioticus Bali G, Pseudomonas corrugata SAJ6, or both, was observed to reduce its overall effectiveness. Survival of the biological agents on leaf surfaces was low, although prior research has indicated that survival may not be essential for sustained disease control. Further investigation is required to determine the potential role of these agents in inducing systemic acquired resistance in crops such as grapes and poppies. For commercial application, repeated treatments may be necessary to maintain disease management. Notably, the protection provided by Trichoderma sp. Td22 was found to be comparable to that of chemical treatments, representing a promising step toward more sustainable agricultural practices.
Korosi Mikroba pada Infrastruktur Logam: Tantangan dan Strategi Pengelolaan Berkelanjutan Ciawi, Yenni; Ramona, Yan
Nata Palemahan: Journal of Environmental Engineering Innovations Vol. 1 No. 2 (2024)
Publisher : Universitas Pendidikan Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38043/natapalemahan.v1i2.5652

Abstract

Korosi mikroba (MIC, microbiologically influenced corrosion) merupakan masalah pelik di berbagai industri, khususnya pada sektor minyak dan gas, infrastruktur kelautan, dan pengolahan air, karena aktivitas mikroba mempercepat degradasi permukaan logam secara signifikan. MIC umumnya oleh bakteri, arkea, dan jamur yang membentuk biofilm pada logam, dan menyebabkan reaksi elektrokimia lokal yang memicu korosi. Tinjauan ini berfokus pada mikroorganisme utama yang terlibat dalam MIC, seperti bakteri pereduksi sulfat (SRB, sulfate-reducing bacteria), bakteri pengoksidasi besi (IOB, iron-oxidizing bacteria), dan metanogen, serta menjelaskan bagaimana faktor lingkungan, seperti ketersediaan oksigen, konsentrasi nutrisi, pH, suhu, dan salinitas, mendukung pertumbuhan mikroba dan korosi logam. Berbagai metode deteksi MIC dievaluasi, seperti analisis mikrobiologi, metode elektrokimia seperti spektroskopi impedansi elektrokimia (EIS, electrochemical impedance spectroscopy), metode uji non-destruktif, serta teknologi sensor real-time. Selain itu, strategi mitigasi MIC, seperti penggunaan material tahan korosi, pelapis antimikroba, biosida, dan sistem perlindungan katodik, dibahas secara rinci. Teknologi baru seperti pelapis pintar (self-healing), nanomaterial, dan sistem bioelektrokimia juga disebutkan sebagai solusi menjanjikan untuk pengelolaan MIC yang lebih efektif dan berkelanjutan. Dengan mengintegrasikan metode deteksi dan mitigasi yang canggih, industri dapat melindungi infrastruktur penting dari dampak jangka panjang korosi mikroba dan mengurangi secara signifikan biaya akibat kerusakan oleh MIC.
Co-Authors A. Line, Martin A.A. Ketut Darmadi Agestiawan, I. G. A. M. Agung Panji Dwipayana, I Dewa Alayna Himawan Anak Agung Gede Indraningrat Anak Agung Ketut Darmadi Anak Agung Ngurah Nara-Kusuma Ananda Sri Phartiswari Andriani, Luh Nyoman Ary Metta Anissa Maria Hidayati Ayu Amelia Bagas Susilo Batho, Yanuarius Felix Cok. Istri Dewiyani Cakrawati Cokorda Istri Sri Arisanti Dandy Bimantara Dewa Ayu Andriastini Dewa Ayu Swastini Diah Kharismawati Djereng Esti Arisetya Dewi Fainmarinat Selviani Inabuy Fernando Putra Flavia Fionessa Falenda Shanzet Fukuda Kenji Gusti Ayu Dianti Violentina Gustu Widi Putra Hani, Ni Luh Helda Thorikul Affan I D.P. Kartika Pratiwi I Dewa Gede Cahyadi Kusuma I Dewa Made Sukrama I G. A. Widagda I Gusti Agung Gde Bayu Wirama I Gusti Ngurah Jemmy Anton Prasetya I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Oka Adi Parwata I Made Saka Wijaya I N.K. Widjaja I Nengah Sujaya I Putu Aditya Prayoga I Putu Oka Suartama I W BUDIARSA SUYASA I Wayan Suanda I Wayan Suardana I. M. A Sasmitha I.B. Oka Suyasa I.D.P.K. Pratiwi IBG Darmayasa Ida Ayu Ketut Ariningsih Ida Ayu Putu Suryanti Ida Ayu Sri Sinca Maha Uni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara Inabuy, Fainmarinat selviani Irma Damayanti K.R Suciptha Kadek Wegi Kurnilia KOMANG AYU NOCIANITRI Komang Dharmawan Kusuma, I Dewa Gede Cahyadi Meitini Proborini Wahyuni N. N. Rupiasi N.G.A Manik Ermayanti N.W.A. Utami Nadia Khairunnisa Nadya Treesna Wulansari Ni LUH ARPIWI Ni Luh Made Ema Juliasari Ni Luh Putu Ariwathi Ni Luh Suriani Ni Made Susilawati Ni Made Teriyani Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Rupiasih Ni Putu Adriani Astiti Ni Putu Desy Aryantini Ni Putu Eka Leliqia Ni Putu Indiani Sahaning Karna Ni Putu Linda Laksmiani Ni Putu Suariani Ni Putu Widarini Ni Putu Widiantari Ni Wayan Eka Putri Gayatri Kastawa Ni Wayan Nursini NYOMAN SEMADI ANTARA Pande Gde Sasmita Julyantoro Pande Putu Ayu Sukmawati Putu Eka Sudaryatma Putu Rima Sintyadewi Retno Kawuri SANG KETUT SUDIRGA Sentot Joko Raharjo Septiari, I Gusti Ayu Agung Shetty, Kalidas Sri Wahyuni Sumartin N.W.E. Tadashu Urashima Teriyani, Ni Made Viviandari, I D. A. A Wayan Redi Aryanta Wulandari, Kristina Yenni Ciawi Yoshitake Orikasa Yuji Oda Yulidia Iriani Yulidia Iriani Yustiantara, Putu Sanna Zulfa, Rozifatul