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Detection of Brucellosis in Dairy Cattle in Turen District Malang Regency using Rose Bengal Test (RBT) and Complement Fixation Test (CFT) Methods Kusumastuti, Imbi; Tyasningsih, Wiwiek; Praja, Ratih Novita; Suwarno, Suwarno; Yunita, Maya Nurwartanti; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.42-47

Abstract

The purpose of this research was to detect Brucellosis in female dairy cattle in Turen District, Malang Regency. A total of 78 samples were used in this research taken from 351 total population of female dairy cattle that have been calculated based on the slovin formula. The methods used in this research were Rose Bengal Test (RBT) for the screening test and continued with Complement Fixation Test (CFT) for the confirmation test. The results RBT of this research clearly showed that positive results from 10 samples were confirmed as a final diagnostic test of Brucellosis using CFT. CFT showed a positive result from 9 samples and negative in 1 sample. The percentage of Brucellosis occurrence in the Turen District was recorded at 11.5%. Moreover, Turen District also became an area with a high Brucellosis occurrence (>2%).
Gamal (Gliricidia Sepium) Leaf Extract Increase Mortality of Fasciola gigantica In Vitro Zulianti, Brillia; Hamid, Iwan Sahrial; Yudhana, Aditya; Rahmahani, Jola; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.193-198

Abstract

Fasciolosis is a disease caused by infection with liver fluke (Fasciola sp). Fasciolosis is a parasitic disease that is important because it can cause high economic losses. The aim of this study was to investigate the effect of gamal (Gliricidia sepium) leaf extract against mortality of Fasciola gigantica in vitro study. In present study was completely randomized design with six treatments and each treatment were divided into four replications. A total of 240 specimens from infected cattles were used in this study. The observation and recording of F. gigantica mortality in all treatments with concentrations of gamal leaf extract P1 (1%), P2 (2%), P3 (3), P4 (4%), K + (Nitroxynil 2%) and K- (physiological NaCl) rate was done in 30, 60, 90, 120, 150, 180, 210 and 240 minutes, respectively. The mortality of F. gigantica was confirmed if there was no movement when triggered by anatomy tweezers then dipped in slightly warm water (50 15í¢„Æ’"> ). The data was analyzed using ANAVA Faktorial and continued with Duncan Multiple Range Test. The results of this study were shown the lowest and the highest mortality rate recorded at minutes 30 and 240 respectively. Moreover, the mean of mortality rate were peaked in nitroxynil (K+) and 1% gamal leaf extract treatment, while the lowest mean of mortality rate occurs in NaCl (K-) treatment. Present study conclude that gamal leaf extract has anthelmintic effect on the mortality rate of Fasciola gigantica. Gamal leaf extract with 1% concentration was claimed to be the most effective.
Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test Kusuma, Agung Jati; Safitri, Erma; Praja, Ratih Novita; Tyasningsih, Wiwiek; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.199-206

Abstract

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi Wijaya, Achmad Arby; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Tyasningsih, Wiwiek; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.207-212

Abstract

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora) Al-Zuhroh, Tisya Yumn; Santoso, Kuncoro Puguh; Yunita, Maya Nurwartanti; Hidajati, Nove; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.213-220

Abstract

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.
The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi Ningrum, Indah Puspita; Soeharsono, Soeharsono; Wibawati, Prima Ayu; Dhamayanti, Yeni; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.48-53

Abstract

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.
Risk Factors and Scabious Prevalence in Cats During 2020 in Griya Satwa Clinic, Magetan Cahya, Nedriana; Primarizky, Hardany; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.81-86

Abstract

Scabious is a disease that often infests cats, originating from the family Sarcoptidae. This study aimed to determine the prevalence and factors that can affect the risk of scabious in cats. The study was evaluated at the Griya Satwa Clinic, Magetan in January-February 2021. The design of this study was a retrospective cohort study by taking secondary data during the 2020 period as study material. The data obtained were then entered into the Statistical Package for the Social Sciences (SPSS) version 20 for windows to analyze the association's relationship and increased risk by calculating the Chi-Square and Odds Ratio. The samples obtained were 76 cats infested with scabious. The analysis of the association of risk factors for scabious was breed (P = 0.428), age (P = 0.793), sex (P = 0.426). The results of the prevalence of scabious in cats from the study during January-February 2021 in the 2020 period were 9.5%, and the analysis of the association (Chi-Square) and the increased (Odds Ratio) of factors including breed, age, sex on the risk of scabious in cats were not significance / absent influence, The probability of error obtained is outside the tolerance set by the researcher, so the results are not significant (p>0,05).
Risk Factors of Parvovirus in Dogs at De' Lab Pet Clinic, Bekasi Harelas, Genoveva Callista; Arimbi, Arimbi; Hamid, Iwan Sahrial; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.170-177

Abstract

This study aimed to determine the percentage of parvovirus, risk factor analysis, and describe the symptoms and treatment of parvovirus. The study was conducted by surveying, collecting secondary data from patient medical records and Bekasi City's rainfall data, data recording, data unification, and data analysis. Data analysis was presented descriptively and tested using Chi-Square and Odds Ratio (OR). The results shows the percentage of parvovirus was 2%. The rainfall factor <150mm/month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,154 times. The age factors 0-6 month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,017 times. The breed factors not significant to the risk of parvovirus (p>0,05) and don't increase the risk of parvovirus. The main symptoms seen in parvovirus include vomiting, diarrhea especially bloody diarrhea, and fever. The main components treatments are fluid therapy, antibiotic, antiemetic, and nutritional support. The administration of other drugs can be given to dogs whose administration must be adjusted to their respective conditions.
Isolation and Identification of Salmonella Spp. on Broiler Meat at Banyuwangi and Blambangan Traditional Markets, Banyuwangi Regency Putri, Diana Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.219-224

Abstract

Salmonella spp. is one of the major causes of the world's foodborne disease that can spread through meats, eggs, or milk. Salmonella spp. is an enteropathogenic bacteria that can cause gastrointestinal infections. This study aimed to identify the contaminations of Salmonella spp. bacteria on the broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district. The method of this study used 24 samples of broiler meat bought from Banyuwangi and Blambangan traditional markets in the Banyuwangi District. The samples were collected using the purposive sampling method. The results found that 5 out of 24 samples (21%) were tested positive for Salmonella spp. contamination and 19 were tested negative. This study was indicated that broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district have not suitable with SNI (Indonesia's National Standard) 3924:2009 that requires at least 25 gram of fresh chicken meat to be free of or tested negative for Salmonella.
Risk Factors and Incidence of Feline Lower Urinary Tract Disease (FLUTD) in Sahabat Satwa Genteng Animal Clinic Plumeriastuti, Hani; Novitasari, Seline Putri; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.15-20

Abstract

The purpose of this study was to determine the risk factors and incidence of Feline Lower Urinary Tract Disease (FLUTD) at the Sahabat Satwa Genteng clinic. A total of 36 samples were evaluated according to research procedures with diagnosis, data collection, urine sampling, dipstick and sediment examination methods, and Chi-square data analysis. The results showed that there was a association between gender, history of castration, and diet on the risk of FLUTD. Meanwhile, there was no association between age and the risk of FLUTD. Clinical signs that often occur in FLUTD disease initiate from stranguria, hematuria, dysuria, periuria, and polykisuria. The results of the dipstick examination founded that 86,1% of cats affected by FLUTD had a normal pH and 13,9% had an alkaline pH. The results of the specific gravity (SG) of cats with FLUTD have values below normal while the protein levels found in all cats suffering from FLUTD have values above normal. Stranguria was found in 69,4% of cats with FLUTD. Leukocytes were also found in all the urine of cats suffering from FLUTD. The most common results of sediment examination were struvite, calcium oxalate, and cystine crystals.
Co-Authors Agustono, Bodhi Al arif, Mohammad Anam Al-Zuhroh, Tisya Yumn Arimbi Ashari, Lukman Baroroh, Diana Nova Cahya, Nedriana Cahya, Rizka Wulan Chozin, Aminullah Alhady Chusniati, Sri Dayanti, Efin Windi Epy Muhammad Luqman Erma Safitri ERMA SAFITRI Faisal Fikri Faizal Ulhaq Fania Aulia Rahma Farodina Azka Zhafira Fatimatus Zahro, Fatimatus Fiqih, Adam Fahmi Hamid, Iwan Sharial Hani Plumeriastuti Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas IMAM MUSTOFA Indaka Rachmach Choirunnisa iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Iwan Sahrial Hamid Kuncoro Puguh Santoso Kuncorojakti, Suryo Kusuma, Agung Jati Kusumastuti, Imbi Lake, Werenfridus Kono Lita Rakhma Yustinasari Megawati, Neneng Ismi Mirza Atikah Madarina Hisyam Moch. Ilham Riza Fahlefi Muhammad Thohawi Elziyad Purnama Murjianingsih, Fitriani Mustofa Helmi Effendi Nenny Harijani Nesa Putri Candra Cahyani Nina Amalia, Nina Ningrum, Indah Puspita Nove Hidajati Novitasari, Seline Putri Nusdianto Triakoso Praja, Ratih Novita Prameswari, Febriana Hawa Prameswari, Ratna Ajeng Prastiya, Ragil Angga Pratika, Selva Primarizky, Hardany Putri, Diana Novita Putrianirma, Rizki Rahmadani, Aurel Aulia Rahmahani, Jola Rahmawati, Anlisia Salahuddin, Muhammad Soleh Salsabila Putri, Elma Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Shelly Oktania Nurvita Sekarsari, Dara Ayu Pramesti Shabrina Fauzia Prayoga Soeharsono Soeharsono Solikhah, Tridiganita Intan Subagyo, Safira Fauziyyah Suciyono, Suciyono Suherni Susilowati Suryanie Sarudji Suwarno Suwarno Trilas Sardjito Tyasningsih, Wiwiek Wardana, Prasvita Rosa Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wijaya, Achmad Arby Wijaya, Nanik Sianita Wilujeng, Estiary Yeni Dhamayanti Yudhana, Aditya Zhafira, Farodina Azka Zhaza Afililla Zulianti, Brillia