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Isolation and Identification of Salmonella Spp. on Broiler Meat at Banyuwangi and Blambangan Traditional Markets, Banyuwangi Regency Putri, Diana Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.219-224

Abstract

Salmonella spp. is one of the major causes of the world's foodborne disease that can spread through meats, eggs, or milk. Salmonella spp. is an enteropathogenic bacteria that can cause gastrointestinal infections. This study aimed to identify the contaminations of Salmonella spp. bacteria on the broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district. The method of this study used 24 samples of broiler meat bought from Banyuwangi and Blambangan traditional markets in the Banyuwangi District. The samples were collected using the purposive sampling method. The results found that 5 out of 24 samples (21%) were tested positive for Salmonella spp. contamination and 19 were tested negative. This study was indicated that broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district have not suitable with SNI (Indonesia's National Standard) 3924:2009 that requires at least 25 gram of fresh chicken meat to be free of or tested negative for Salmonella.
Risk Factors and Incidence of Feline Lower Urinary Tract Disease (FLUTD) in Sahabat Satwa Genteng Animal Clinic Plumeriastuti, Hani; Novitasari, Seline Putri; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.15-20

Abstract

The purpose of this study was to determine the risk factors and incidence of Feline Lower Urinary Tract Disease (FLUTD) at the Sahabat Satwa Genteng clinic. A total of 36 samples were evaluated according to research procedures with diagnosis, data collection, urine sampling, dipstick and sediment examination methods, and Chi-square data analysis. The results showed that there was a association between gender, history of castration, and diet on the risk of FLUTD. Meanwhile, there was no association between age and the risk of FLUTD. Clinical signs that often occur in FLUTD disease initiate from stranguria, hematuria, dysuria, periuria, and polykisuria. The results of the dipstick examination founded that 86,1% of cats affected by FLUTD had a normal pH and 13,9% had an alkaline pH. The results of the specific gravity (SG) of cats with FLUTD have values below normal while the protein levels found in all cats suffering from FLUTD have values above normal. Stranguria was found in 69,4% of cats with FLUTD. Leukocytes were also found in all the urine of cats suffering from FLUTD. The most common results of sediment examination were struvite, calcium oxalate, and cystine crystals.
Retrospective Study of Feline Panleukopenia Risk Factors in Pet Cats in Madiun Baroroh, Diana Nova; Tyasningsih, Wiwiek; Praja, Ratih Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.114-119

Abstract

This study analyzed the association between risk factors and Feline panleukopenia at the Ichiyo Veterinary Clinic, Madiun using a retrospective method. A total of 2037 data used was secondary data obtained from cat medical and ambulatory reports from January 2018-March 2021. The risk factors analyzed included age, gender, vaccination status, comorbid diseases, housing system, number of cats, and season. Data was presented descriptively and analyzed using Chi-Square analysis. The results showed that the risk factors for Feline panleukopenia were cats aged ≤6 months, cats that were not vaccinated, and cats with comorbid diseases. It can be concluded that prevention of Feline panleukopenia can focus on age, vaccination and treatment of potential comorbid diseases in cats.
Prevalence and Risk Factors of Gastrointestinal Helminth Infection in Pet Cats in Wonogiri District, Central Java Sekarsari, Dara Ayu Pramesti; Saputro, Amung Logam; Agustono, Bodhi; Yunita, Maya Nurwartanti; Praja, Ratih Novita; Solikhah, Tridiganita Intan
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49559

Abstract

Gastrointestinal helminth infection is the most common and the easiest infection to infect wild cats or pet cats but is often ignored by the cat owners because sometimes there are no symptoms or a lack of attention from the cat owner to their pet cat. The aim of this study was to estimate the prevalence and risk factors of gastrointestinal helminth infection in pet cats in Wonogiri District, Central Java. A total of 100 cat samples were evaluated using a questionnaire, then fecal microscopic examination using simple native method, sedimentation, and floatation methods. Data obtained from this study were presented descriptively and analyzed using the Chi-Square test. The result showed that 37 out of 100 fecal samples were found positive infected with gastrointestinal helminth including Toxocara cati and Dipylidium caninum or the prevalence of gastrointestinal helminth infection can be calculated at 37%. The result of the analysis of significant risk factors were breed, number of pet cat owners, grooming, deworming, and vaccination.
The Effect of Adding Probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactococcus lactis on The Quantity and Quality of Layer Eggs Isa Brown Chozin, Aminullah Alhady; Yunita, Maya Nurwartanti; Dhamayanti, Yeni; Kuncorojakti, Suryo; Prastiya, Ragil Angga; Wibawati, Prima Ayu
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51252

Abstract

Laying breeds of chickens or commonly called layers are a breed of chicken that has potential value as an egg producer. The length of the layer production period is 80 – 90 weeks. During layer production, layers require high energy to produce, while the level of energy in the ration determines layer consumption. Various efforts have been made to increase the efficiency of using chicken feed in tropical areas, one of which is the use of additional feed additives. Feed additives that can be used are probiotics which come from microorganisms such as Lactobacillus acidophilus, Lactobacillus plantarum and Lactococcus lactis. This research aims to determine the effect and potential of adding a combination of probiotics Lactobacillus acidophilus, Lactobacillus plantarum, and Lactococcus lactis in increasing the quantity and quality of layer eggs during the production period. This research is experimental using a Completely Randomized Design (CRD). This study used a layer population of 20 individuals from the Isa Brown strain aged 40 weeks which were divided into four groups consisting of P1, P2, P3 and P4 with each group consisting of 5 individuals. The data that was collected was analyzed using Analysis of Variance (ANOVA) using SPSS version 24.0 software, then continued with the Duncan test on the data which showed significant differences (P<0.05). Based on the results of the study, the addition of the probiotics Lactobacillus acidophillus, Lactobacillus plantarum, and Lactococcus lactis in drinking water at doses of 2 mL/L, 4 mL/L, and 6 mL/L cannot increase the quantity of layer eggs, but can improve the external quality of layer eggs (egg weight, egg length, egg diameter, shell weight, shell thickness), and can improve the internal quality of layer eggs (yolk score, egg yolk index, egg white index, and haugh unit).
Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) iqbal dwi warsito; Suherni Susilowati; Erma Safitri; Ragil Angga Prastiya; Maya Nurwartanti Yunita; Amung Logam Saputro
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.16-24

Abstract

This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.
Efektivitas Kitosan dari Limbah Kulit Udang terhadap Angiogenesis dalam Penyembuhan Luka Eksisi pada Tikus Putih (Rattus norvegicus) Jantan Dayanti, Efin Windi; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Plumeriastuti, Hani; Purnama, Muhammad Thohawi Elziyad; Wibawati, Prima Ayu
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.60-69

Abstract

Luka eksisi adalah luka yang disebabkan akibat terpotongnya jaringan oleh benda yang tajam (Partogi, 2008). Kitosan mampu sebagai zat dalam penyembuhan luka salah satunya dalam proses angiogenesis. Tujuan penelitian ini yaitu untuk mengetahui efektivitas salep kitosan dari limbah kulit udang terhadap angiogenesis dalam penyembuhan luka eksisi pada tikus putih jantan. Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K+ luka eksisi dengan pemberian povidone iodine, K- luka eksisi dengan pemberian salep tanpa kitosan, P1 luka eksisi dengan pemberian salep kitosan 1,5%, P2 luka eksisi dengan pemberian salep 2,5% dan P3 luka eksisi dengan pemberian salep kitosan. Terapi diberikan sehari sekali selama tujuh hari. Hasil data rata-rata jumlah pembuluh darah baru dianalisis dengan uji Anova yang menunjukkan adanya perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji LSD (Least Significant Differences). Kelompok P1 dan P2 tidak memiliki perbedaan yang nyata, tetapi berbeda nyata dengan kelompok K+, K- dan P3. Kesimpulan dari penelitian ini kitosan efektif dalam meningkatkan jumlah pembuluh darah baru pada proses penyembuhan luka eksisi.
Effect of Coffea canephora Bean Extract on Lead Acetate-Induced Rats Moch. Ilham Riza Fahlefi; Maya Nurwartanti Yunita; Indaka Rachmach Choirunnisa; Farodina Azka Zhafira; Fania Aulia Rahma
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.97-104

Abstract

Lead (Pb) is a non-essential heavy metal that is toxic and has no known uses. Most cases of poisoning in livestock are caused by lead poisoning. This study aims to determine the effect of Coffea canephora bean extract as an antioxidant on the heart histopathological features of white rats (Rattus norvegicus) induced by lead acetate. This study used 25 male Wistar rats aged between eight and ten weeks and weighing between 200 and 250 grams. The rats were divided into five treatment groups, namely negative control (C-), positive control (C+), treatment one (T1), treatment two (T2), and treatment three (T3).  The results suggested that the administration of 200 mg/kg BW, 400 mg/kg BW, or 800 mg/kg BW of Coffea canephora bean extract was not effective in improving the heart histopathological features, including cell degeneration, hemorrhage, and necrosis, of rats induced with lead acetate at a dose of 2 mg/kg BW.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Yudhana, Aditya; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
Effects of Nicotine-Containing E-Cigarette Smoke on Testicular Histopathology in Albino Rats Amalia, Nina; Zhafira, Farodina Azka; Yunita, Maya Nurwartanti; Sari, Shelly Oktania Nurvita; Herdiansyah, Akbar Dimas; Pratika, Selva; Wardana, Prasvita Rosa; Zahro, Fatimatus; Rahmadani, Aurel Aulia
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.82605

Abstract

This study aims to assess the level of damage from histopathology of seminiferous tubules of testicular organs of albino rats affected by exposure to e-cigarette vapor. The 20 albino rats used were divided into 5 groups, which are the control group (C) not given exposure to e-cigarette vapor and the treatment group given exposure to e-cigarette vapor at different doses. Treatment groups T1 (0.3 mg/ml), T2 (3 mg/ml), T3 (12 mg/ml), T4 (36 mg/ml). All treatment groups were given perinhaled and the treatment was carried out for 14 days. At the end of treatment, albino rats were euthanized and then necropsied and testicular organs were preserved to make histopathological preparations using Hematoxilin-Eosin staining. The results of the histopathological picture of the seminiferous tubules of the testes were observed at 400x magnification and the thickness of the epithelium and the diameter of the seminiferous tubules. The results showed a significant change in the thickness of the seminiferous tubule epithelium in each treatment which decreased, but there was no significant change in the diameter of the seminiferous tubules. This study states that exposure to e-cigarette vapor in rats can cause a decrease in spermatogenesis.
Co-Authors Agustono, Bodhi Al arif, Mohammad Anam Al-Zuhroh, Tisya Yumn Amung Logam Saputro Amung Logam Saputro Arimbi Ashari, Lukman Baroroh, Diana Nova Cahya, Nedriana Cahya, Rizka Wulan Chozin, Aminullah Alhady Chusniati, Sri Dayanti, Efin Windi Erma Safitri ERMA SAFITRI Faisal Fikri Fania Aulia Rahma Farodina Azka Zhafira Fatimatus Zahro, Fatimatus Fiqih, Adam Fahmi Hamid, Iwan Sharial Hani Plumeriastuti Harelas, Genoveva Callista Harijani, Nenny Hayati, Laila Nur Herdiansyah, Akbar Dimas Hidajati, Nove IMAM MUSTOFA Indaka Rachmach Choirunnisa iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Kuncoro Puguh Santoso Kuncorojakti, Suryo Kusuma, Agung Jati Kusumastuti, Imbi Lake, Werenfridus Kono Megawati, Neneng Ismi Moch. Ilham Riza Fahlefi Muhammad Thohawi Elziyad Purnama Murjianingsih, Fitriani Mustofa Helmi Effendi Nina Amalia, Nina Ningrum, Indah Puspita Nove Hidajati Novitasari, Seline Putri Nusdianto Triakoso Praja, Ratih Novita Prameswari, Febriana Hawa Prameswari, Ratna Ajeng Prastiya, Ragil Angga Pratika, Selva Primarizky, Hardany Putri, Diana Novita Putrianirma, Rizki Rahmadani, Aurel Aulia Rahmahani, Jola Rahmawati, Anlisia Salahuddin, Muhammad Soleh Salsabila Putri, Elma Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Shelly Oktania Nurvita Sarudji, Suryanie Sekarsari, Dara Ayu Pramesti Shabrina Fauzia Prayoga Soeharsono Soeharsono Solikhah, Tridiganita Intan Subagyo, Safira Fauziyyah Suherni Susilowati Suwarno Suwarno Trilas Sardjito Tyasningsih, Wiwiek Wardana, Prasvita Rosa Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wijaya, Achmad Arby Wijaya, Nanik Sianita Wilujeng, Estiary Yeni Dhamayanti Yudhana, Aditya Yustinasari, Lita Rakhma Zhafira, Farodina Azka Zulianti, Brillia