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Pathological Impacts Due to The Existence of Plastic Waste in Rumen of Bali Cattle Berata , I Ketut; Susari, Ni Nyoman Werdi; Kardena, I Made; Novianti, Arindita Niatazya
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.26-30

Abstract

This study aimed to determine the prevalence of Bali cattle rumen containing plastic waste and its impact on tissues and health risks. A total of 100 Bali cattle were investigated after being slaughtered at several traditional slaughterhouses in Denpasar City. Rumen samples were examined for any plastic waste, meanwhile blood, liver, kidney, lungs, spleen, intestine, and myocardium were collected for evaluation of heavy metals content and histopathological examination. The heavy metal measurement was performed using the atomic absorption spectrophotometry (AAS) method. Those tissues for histopathological examination were fixed in buffer neutral formalin (BNF) and then evaluated using hematoxylin-eosin (HE) staining. The results of the examination reported 9 rumens (9%) containing plastic waste. The hematological profile showed leucocytosis and the content of lead (0.841 ± 0.522 ppm). Histopathological changes in the liver, kidneys, lungs, spleen, and intestine, were found in various types of degeneration, inflammation, and mild necrosis. In conclusion, the prevalence of plastic waste in the rumen of Bali cattle was 9%, with leucocytosis, lead content was 0.841 ± 0.522 ppm, and various histopathological lesions such as congestion, inflammation, and fatty degeneration in parenchymous tissues.
Case Report: Identification of Eimeria tenella Infection and Pathologic Changes in a Broiler Chicken in Kutuh Village Farm, Badung, Bali Putra, I Putu Cahyadi; Sari, Ni Komang Wahyu Centika; Kardena, I Made; Besung, I Nengah Kerta
Mandalika Veterinary Journal Vol. 5 No. 2 (2025): OKTOBER
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/mvj.v5i2.15522

Abstract

Coccidiosis is a serious parasitic disease of chickens caused by the protozoan Eimeria, with Eimeria tenella being one of the most virulent species. The infection causes necrosis and hemorrhage of the large intestine, resulting in significant economic losses in the poultry industry. The purpose of this case report was to determine the cause of death of broiler chickens in one farm in Kutuh Village, South Kuta Subdistrict, Badung Regency. The case animal (protocol number 39/N/24) was a 20-day-old broiler from the farm with a population of 18,000 birds. The examination process included anamnesis, clinical signs, anatomical pathology, histopathology, bacteriology, parasitology (including coprology and McMaster techniques), and morphometric identification of Eimeria tenella using ImageJ software. The clinical signs observed included anorexia, weakness, dull feathers, and reddish feces with a porridge-like consistency. Pathological examination revealed alterations in the cecum and colon. Histopathological analysis indicated typhlitis hemorrhagis et necrotican and colitis necrotican. Bacteriological examination revealed Escherichia coli, a normal flora, in the caecum and colon. Morphological and morphometric analyses of the oocysts revealed that E. tenella is ovoid, with two wall layers, an average size of 21.670 × 19.942 μm, and an oocyst count of 188,600 per gram of feces (OPG). The cause of death of the case chickens was due to severe Eimeria tenella infection.
Identification and Pathological Finding of Colisepticemia in Broiler Indra, Rusmin; Kardena, I Made; Suarjana, I Gusti Ketut
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The chicken was sick for 6 days, weak and disrupted growth. Necropsy confirmed that several organs was bleeding and containing fibrin exudation. The results of histopathological examination showed the presence of necrotic encephalitis, hemorrhagic tracheitis, hemorrhagic and necrotic pneumonia, hemorrhagic epicarditis and myocarditis, hemorrhagic enteritis, hemorrhagic and necrotic hepatitis, also hemorrhagic and necrotic glomerulonephritis. Microbiological examination confirmed the discovery of Eschericia coli bacterial agents that can hemolyze blood in the heart, lungs, and intestines. Parasitological examination confirmed that no parasitic agent was involved in this case. From this case study, it can be concluded that the single agent that causes pathological changes was found in the form of pathogenic E. coli bacteria (colisepticemia) which was exacerbated by unfavorable farming conditions. Keywords: Escherichia coli, colisepticemia, broiler, pathological findings
PENEGUHAN DIAGNOSIS PENYAKIT NEWCASTLE DISEASE LAPANG PADA AYAM BURAS DI BALI MENGGUNAKAN TEKNIK RT-PCR Kencana, Gusti Ayu Yuniati; Kardena, I Made; Kade Mahardika, I Gusti Ngurah
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.349

Abstract

Penelitian ini bertujuan mendiagnosis kasus newcastle disease (ND) lapangan pada ayam bukan ras yang bersifat akut melalui hasil pemeriksaan di Laboratorium Patologi dan Laboratorium Biomedik Fakultas Kedokteran Hewan Universitas Udayana tahun 2008-2009. Sebanyak sepuluh ekor sampel ayam buras telah diperiksa. Gejala klinis yang teramati meliputi: anoreksia, lesu, bersin, batuk, dan diare putih kehijauan dengan diagnosis sementara sebagai penyakit ND yang bersifat akut. Sampel diambil dari organ yang mengalami perubahan patognomonis seperti pada proventrikulus, ventrikulus, seka tonsil, paru-paru dan otak. Perbanyakan virus menggunakan telur ayam bertunas (TAB) umur 10 hari melalui ruang alantois dan diinkubasikan pada inkubator telur suhu 37 C selama 3 hari. Koleksi cairan alantois dilakukan pada hari ke-3, selanjutnya diidentifikasi dengan uji serologi hemaglutinasi (Haemaglutination-Inhibition Test/HA/HI) dengan teknik mikrotiter baku dan dikonfirmasi dengan uji reverse transcriptase-polymerase chain reaction (RT-PCR) menggunakan primer FNDIFP (5-CCCCGTTGGAGGCATAC-3) dan FNDIBP (5-TGTTGGCAGCATTTTGATTG-3). Hasil penelitian menunjukkan bahwa semua sampel kasus ayam bukan ras yang diperiksa positif terinfeksi oleh virus penyakit ND akut. Kajian ini mengindikasikan bahwa penyakit ND di Bali masih bersifat endemis.
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT Berata, I ketut; Winaya, Ida Bagus Oka; Kardena, I Made
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.307

Abstract

Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
Perbandingan GCU (Glucose, Cholesterol, Uric Acid) Hemoglobin Multiparameter dengan Biochemistry Analyzer pada Darah Monyet Ekor Panjang di Monkey Forest Ubud Patricia, Clarissa; Soma, I Gede; Kardena, I Made
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.106178

Abstract

Glukosa, kolesterol, dan asam urat merupakan bagian dari biokimia darah monyet ekor panjang yang dapat diperiksa menggunakan alat penunjang berupa GCU (glucose, cholesterol, uric acid) hemoglobin multiparameter, akan tetapi, alat ini sebelumnya belum pernah digunakan untuk menguji darah monyet ekor panjang karena alat tersebut ditujukan untuk manusia. Penelitian ini bertujuan untuk mengetahui perbandingan nilai uji biokimia darah (glukosa, kolesterol, dan asam urat) menggunakan GCU hemoglobin multiparameter dengan biochemistry analyzer yang biasa digunakan sebagai acuan di laboratorium klinik hewan. Darah monyet ekor panjang diambil dari ujung jari untuk sampel yang diuji dengan GCU hemoglobin multiparameter dan dari vena femoralis untuk sampel yang diuji dengan biochemistry analyzer. Data diolah dengan uji T berpasangan, sehingga hasil perbandingan nilai dari alat GCU hemoglobin multiparameter dan biochemistry analyzer pada darah monyet ekor panjang menunjukkan perbedaan yang signifikan (p < 0.05).
HEPATIC HISTOPATHOLOGY OF WHITE RATS EXPOSED TO LEAD AND TREATED WITH MORINGA OLEIFERA LEAF SIMPLICIA Wuriandari, Paula Bintang; Berata, I Ketut; Winaya, Ida Bagus Oka; Kardena, I Made; Setiasih, Ni Luh Eka; Samsuri; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p18

Abstract

Lead (Pb) is a heavy metal that induces the formation of free radicals and causes hepatocellular damage, making the liver one of the primary target organs of lead toxicity. Moringa oleifera leaves contain natural antioxidants with the potential to counteract oxidative stress induced by lead exposure. This study aimed to evaluate the effect of Moringa oleifera leaf simplicia on liver histopathology in white rats (Rattus norvegicus) exposed to lead acetate. A total of 20 rats were randomly divided into four groups: P0 (negative control; standard feed and water), P1 (positive control; 0.5 mg/head/day of lead acetate for 3 days), P2 (0.5 mg/head/day of lead acetate plus 0.1 g/mL/day of Moringa oleifera leaf simplicia), and P3 (0.5 mg/head/day of lead acetate plus 0.6 g/mL/day of Moringa oleifera leaf simplicia). Treatments were administered for 14 days via intragastric gavage. On day 15, the rats were euthanized, and liver tissues were collected, processed histologically, and stained with hematoxylin-eosin. Histopathological lesions, including congestion, fatty degeneration, and necrosis, were scored on a scale from 0 to 3 based on severity. The Kruskal-Wallis test showed significant differences among groups for all lesions (P < 0.05). The Mann-Whitney test revealed significant differences in hepatic congestion between P0 and P1, P0 and P2, as well as between P3 and P1-P2, while no significant difference was observed between P0 and P3. For fatty degeneration and necrosis, P0 differed significantly from P1, P2, and P3. In conclusion, Moringa oleifera leaf simplicia at a dose of 0.6 g/mL/day reduced the severity of hepatic congestion caused by lead exposure; however, its effects on fatty degeneration and necrosis were not statistically significant.
Studi Kasus: Koksidiosis Bentuk Intestinal dan Sekum Disertai Dugaan Infeksi Virus pada Ayam Broiler di Kecamatan Ungasan, Bali Putri, Rindar Mentari Nusanti; Putra, I Putu Cahyadi; Kardena, I Made; Suarjana, I Gusti Ketut; Astawa, I Nyoman Mantik
Jurnal Veteriner Nusantara Vol 9 No 1 (2026): Februari, 2026
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v9i1.27275

Abstract

Coccidiosis, Avian Influenza (AI), and Newcastle Disease (ND) are important diseases in broiler chickens that can cause economic losses. This case report aimed to identify the causative agents and describe the pathological changes in broiler chickens suspected of having coccidiosis accompanied by viral infection in the Ungasan District of Bali. The broiler chickens were approximately 25 days old and had bloody diarrhea. The examinations included anamnesis, epidemiological investigation, anatomical pathology, histopathology, parasitology, and bacteriology. Based on epidemiological data, morbidity was 0.7%, mortality was 0.2%, and the case fatality rate was 30% of the total population of 14,000 birds. Anatomical pathology and histopathological examinations revealed changes in almost all organs, indicating infection with the AI and/or ND virus. Necrosis, hemorrhage, inflammatory cell infiltration, and clusters of Eimeria spp. at various life stages were observed in the intestines and caecum. Qualitative and McMaster fecal examinations revealed the presence of Eimeria spp. oocysts at a rate of 132,700 oocysts/gram. Bacteriological examination identified the growth of Escherichia coli, but without any indication of secondary infection from bacteria because Escherichia coli is the normal flora of the intestine. Based on these examination results, it can be concluded that the chickens were infected with intestinal and cecal coccidiosis caused by Eimeria spp., accompanied by suspected ND and/or AI.
Co-Authors Abdul Azis Nasution Abriansyah, Mohammad Ghaiz Adiluhur, I.M.P. Agastia, I.P.A. Agastia, P. A. A. Aida Lousie Tenden Rompis Amelia Avianti Saritjang Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Gede Sugianthara Anggreni, Ni Kadek Wiwik Annabella Ruth Wijaya Annas Farhani Arhiono, Haru Nira Putra Arini Nurhandayani Bambang Pontjo Priosoeryanto Bayu Setiabudi Berata , I Ketut Boedi P. Yenniastuti, Boedi P. Calvin Iffandi Calvin Iffandi Chanda Preanger, Chanda Claudia Murti Andari Desak Nyoman Dewi Indira Laksmi Desi Elrini Sarah Alunat Deska Gilang Mustika Dewa Ayu Swastini Dewa Ayu Widia Kusuma Ningrat, Dewa Ayu Widia Kusuma Dewi, I. A. M. Dewi, I.A.M.K. DWI SURYANTO Edward Emanuel Mango Eldarya Envisari Depari Elyda . Endris Arif Wicaksono, Endris Arif Farhan Abdul Hasan, Farhan Abdul Fathurohman Nasrudin Ferbian M. Siswanto, Ferbian M. Ferbian Milas Siswanto Findri Andriani, Findri Fitri Irawan Rahmawandani Gunawan, I Wayan Nico Fajar Gusti Ayu Yunianti Kencana Gusti Ayu Yuniati Kencana Hendrina Konda M Meha, Hendrina Konda M Herbert . Herbert . Hidayatullah, Lalu Syarif I Dewa Gde Tara Damayanthi, I Dewa Gde Tara I Dewa Made Adhiwitana I Gede Hendra Prasetya Wicaksana I Gede Soma I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Gunata I Ketut Puja I Ketut Suatha I Made Damriyasa I Made Dodi Gunawan I Made Dwinata I Made Merdana I Made Sukada I N. T. ARIANA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Cahyadi Putra, I Putu Cahyadi I Putu Gede Yudhi Arjentinia I Putu Wiralaga I Wayan Batan I Wayan Gorda I Wayan Suardana I Wayan Sudiarta I Wayan Sudira I Wayan Wirata I. H. U Utama I.K. Berata Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Imam Sobari imam sobari Indahyani, Ni Made Dwi Indra, Rusmin Indrawan, Hieronimus Irhas, Rajiman Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Jihadulhaq, Jihadulhaq Kadek Karang Agustina Kartika, Erena Hajar Ketut Budiasa Ketut ELI Supartika Ketut Santhia Adhy Putra Ketut Tono Pasek Gelgel Komang Andika Purnama Komang Ariya Hendrayana Kristi Agusti Putri, Kristi Agusti Lagho, Engelbertus Efraim Luh Gde Sri Surya Heryani Luh Made Sudimartini Mahadewi, S. A. Mahadewi, S.A. Mahyuzar Mahyuzar Muhamad Furkam Fadilah Nallakrishna, I P. A. Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Watiniasih Ni Made Rina Yulinta Ni Nyoman Werdi Susari Ni Putu Ariantari Ni Putu Trisna Asih Ni Wayan Apsari Shantika Pratistha Ni Wayan Listyawati Palgunadi Ni Wayan Listyawati Palgunadi Novianti, Arindita Niatazya Noviriolla Maria Nugraha, Elisabeth Yulia Nur Hanifah Septiani, Nur Hanifah Nurrohman, Fahmi Galuh Nurul Faiziah Nurul Faiziah P. A. C. Kusuma P. A. C. Kusuma, P. A. C. P. Sampurna Paranitha, Dewa Ayu Patricia, Clarissa Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Prabandari, Anak Agung Istri Vera Pranatha, Wahid Danang PRANSIKA EKSY YONITA Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Purwani, S. T. D Purwani, S. T. D. Putri Udayani Putri, Rindar Mentari Nusanti Putu Agus Trisna Kusuma Antara Putu Dede Yudi Utama, Putu Dede Yudi Putu Mira Puspitayani Rasdiyanah . Rasdiyanah . Ratih, Dwi Kusuma Komala Rui Daniel de Carvalho S. T. D Purwani, Sakura, Yoakim Wilson Samiantara, I. K. Samsuri Samsuri Samsuri Samuel Leonardo Silitonga Sari, Ni Komang Wahyu Centika Septianingsih, Rayni Silaban, Jesiaman Siswahyudianto Sri Kayati Widyastuti Sri Milfa Sri Milfa STEVEN YOHANES BOGIA TJOKORDA ISTRI AGUNG Cintya Dalem Tri A. Putra, Tri A. Tri Adi Putra, Tri Adi Wahyu Semadi Putra Widiarta, Lutfi Widyasanti, Ni Wayan Helpina Wuriandari, Paula Bintang Yanti, I G. A. A. D. Yasunobu Matsumoto Yesi Veronica Sitepu Yoga Eka Prasetyo Yoshihiro Hayashi Yuliantari, Ida Ayu Made Yunita Lestyorini Yunita Lestyorini