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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ni Wayan Kurniani Karja
Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Journal : Hemera Zoa

 1 
PCS-4 In Vivo Embryo Production at Cipelang Livestock Embryo Centre Faisal Amri Satrio; Ni Wayan Kurniani Karja; M Imron; Yuni Siswani; Oloan Parlindungan; Bambang Purwantara
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique.                Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1].                 Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing.  The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang.
PF-17 The Development of Crude Testicular Cells in In Vitro Culture Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Spermatogenesis is a continuous process in which spermatogonial stem cells (SSC) develop into specific germ cells before terminally differentiating to form spermatozoa.  The process is supported by Sertoli cells, which are in close contact with germ cells in the seminiferous tubules. Sertoli cells provide essential hormonal signals, nutrients, and physical support to germ cells for successful spermatogenesis.The crude testicular cells (CTC) contains many cell types, like Sertoli cell, Leydig cell, spermatogonial stem cell (SSC), spermatocyte and other testicular somatic cells (Shah et all. 2016). Testicular cells are believed to secrete various growth factors that induced the spermatogenesis process.  The spermatogonial stem cells are unique population of cells in the male testis, which dual function.  First self-renewing their population to maintain the number of stem cells, secondary function is differentiating into spermatids in testis (Wang et al.  2015).Spermatogenic cells differentiation  needed the similar microenvironment in vivo spermatogenesis.  The essential nutrients was collected from healty culture and the culture contained mixed population of cells both the somatic cells and spermatogenic cells.  To identification the spermatogenic cells using Periodic Acid Schifft (PAS) staining (Chang et al. 2011). The present study examined the development of crude testicular cells using PAS staining.
Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin