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PENGARUH PEMBERIAN EKSTRAK UBI JALAR UNGU DAN EKSTRAK BUAH BIT TERHADAP KADAR KOLESTEROL PADA TIKUS WISTAR YANG DIBERI DIET TINGGI LEMAK Tohjiwa, G. Raditya; Wahyuniari, Ida Ayu Ika; Linawati, Ni Made; Arijana, I G Kamasan Nyoman
E-Jurnal Medika Udayana Vol 11 No 6 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i06.P02

Abstract

Cardiovascular disease is a disease that often occurs in Indonesia. Cholesterol and free radicals can stimulate an increase in oxidative stress on the walls of the coronary arteries which can trigger coronary heart disease. Ipomea Batatas and Beta Vulgaris L contain flavonoids and betalains which is useful to lower cholesterol levels in the body to prevent cardiovascular disease. This study aimed to determine the effect of the combination of purple sweet potato and beetroot extract on cholesterol levels in rats fed a high-fat diet. The study design was an experimental study using the Post Test Only Control Group Design method with a sample of 24 male wistar rats fed a high-fat diet and divided into 4 groups. Aquadest was given to the control group (K-) while Ipomea Batatas extract (P1), Beta Vulgaris L extract (P2) and a combination of Ipomea Batatas and Beta Vulgaris L extract (P3) were given to the treatment group. Cholesterol levels will be tested using photometrics with Diasys Cholesterol reagent using blood serum. Data is analyzed with One Way ANOVA. This research showed that there were significant differences in cholesterol levels between the control group and the treatment group given purple sweet potato extract and combination extract with a P value <0.05. The cholesterol level of Group K- 116.8 mg/dL, P1 88.8 mg/dL, P2 99.0 mg/dL, and P3 92.5 mg/dL In conclusion, purple sweet potato extract and the combination extract could reduce cholesterol levels in male wistar rats fed a high-fat diet. Keywords: Ipomoea batatas, Beta vulgaris L, Cholesterol Levels
EFEK PEMBERIAN TEH KOMBINASI EUPHORBIA MILII DAN PROPOLIS TERHADAP GAMBARAN HISTOPATOLOGI HEPAR TIKUS WISTAR JANTAN Rastika, I Gede Agus; Linawati, Ni Made; Ratnayanti, I Gusti Ayu Dewi; Sugiritama, I Wayan
E-Jurnal Medika Udayana Vol 11 No 5 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i05.P09

Abstract

ABSTRAK Pendahuluan: Indonesia mempunyai sumber daya melimpah yang dapat digunakan sebagai pengobatan tradisional. Dua diantaranya yaitu tanaman Euphorbia milii dan propolis. Euphorbia milii dan propolis mempunyai beragam manfaat seperti antioksidan, antibakteri, dan imunomodulator. Efek toksik dari teh kombinasi Euphorbia milii dan propolisx terhadap hepar belum dilaksanakan penelitian sebelumnya. Tujuan dari dilaksanakannya penelitian ini yaitu untuk mengetahui efek pemberian teh kombinasi dari bunga Euphorbia milii dan propolis terhadap gambaran histopatologi hepar tikus wistar jantan. Metode: Penelitian ini merupakan penelitian analitik eksperimental dengan rancangan penelitian berupa randomized post-test only control group design. Dua puluh empat tikus wistar jantan berusia 8 sampai 12 minggu dengan berat badan ± 0.2 kg dibagi menjadi 4 kelompok, yaitu; kelompok kontrol (pakan standar + aquabidest), P1 (teh kombinasi Euphorbia milii dan propolis 40mg/100grbb selama 2 minggu), P2 (teh kombinasi Euphorbia milii dan propolis 40mg/100grbb selama 4 minggu), dan P3 (teh kombinasi Euphorbia milii dan propolis 40mg/100grbb selama 6 minggu). Hasil: Berdasarkan analisis data dari rerata skoring degenerasi dan nekrosis sel hepatosit, tidak ditemukan perbedaan rerata yang signifikan diantara kelompok kontrol dan kelompok perlakuan P1, P2, dan P3 (p>0,05). Simpulan: Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa pemberian teh kombinasi Euphorbia milii dan propolis dengan dosis 40mg/100grbb tidak menimbulkan kerusakan yang bermakna terhadap hepar tikus wistar jantan. Kata kunci : Teh kombinasi, Euphorbia milii dan propolis, Histopatologi hepar
Tinjauan Literatur: PATOGENESIS DAN TERAPI ATROFI VAGINA Sugiritama, I Wayan; Linawati, Ni Made; Wiryawan, I Gusti Nyoman Sri; Wahyuniari, Ida Ayu Ika; Ratnayanti, Gusti Ayu Dewi; Arijana, I Gusti Kamasan Nyoman
Majalah Kesehatan Vol. 11 No. 2 (2024): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2024.011.02.8

Abstract

Atrofi vagina adalah masalah kesehatan yang paling banyak dialami oleh wanita menopause. Patogenesisnya berkorelasi dengan penurunan kadar hormon estrogen, yang mengakibatkan  epitel vagina lebih tipis dengan proporsi sel-sel superfisial yang menurun. Pada dinding vagina terjadi penurunan jumlah serabut kolagen, penurunan massa otot polos dan penurunan vaskularisasi. Konsekuensi dari perubahan tersebut adalah dinding vagina mengalami penurunan dalam ukuran, elastis, lubrikasi, dan integritas lapisan mukosa. Gejala  yang paling sering dialami adalah rasa nyeri pada vagina, yang akhirnya menyebabkan ketakutan untuk melakukan aktivitas seksual.Penanganan gejala atrofi vagina menjadi penting karena populasi wanita menopause yang besar dan akan terus meningkat. Tujuan penulisan tinjauan literatur adalah mengulas patogenesis atrofi vagina dan berbagai macam pilihan terapinya. Literatur didapatkan melalui pencarian pada Google Scholar dan PubMed database selama tahun 2022 dan dipilih terbitan 10 tahun terakhir, baik dalam Bahasa Indonesia maupun Bahasa Inggris. Kata kunci yang digunakan adalah menopause, atrofi vagina, patogenesis atrofi vagina, terapi atrofi vagina, fitoestrogen, Selective Estrogen Estrogen Receptors (SERM), dan terapi sulih hormon. Hasil menemukan bahwa sebagian besar kasus tidak terdiagnosis dan hanya sedikit yang mendapat terapi yang tepat. Sebagian besar wanita menopause mengobati sendiri gejala atrofi vagina yang dialami dengan obat-obat yang dijual bebas dan ada banyak kasus yang tidak diobati. Terapi utama atrofi vagina adalah hormon yang diberikan secara sistemik atau topikal. Salah satu terapi sistemik adalah SERM. Terapi topikal pilihannya sangat banyak yaitu fitoestrogen, asam hialuronat, pelembab, pelumas, kolostrum, vitamin, herbal, terapi laser dan radio frekuensi. Berbagai jenis metode terapi memiliki mekanisme kerja yang berbeda, sebagian besar terbukti efektif mampu memperbaiki struktur dan fisiologi vagina serta mampu mengurangi gejala atrofi vagina dan juga memperbaiki fungsi seksual.
Ekstrak Etanol Ubi Jalar Ungu untuk Mencegah Peningkatan pH Vagina setelah Ovariektomi Bilateral pada Tikus Wistar Sugiritama, I Wayan; Sri Wiryawan, I Gusti Nyoman; Linawati, Ni Made; Wahyuniari, Ida Ayu Ika; Ratnayanthi, I Gusti Ayu Dewi; Arijana, I Gusti Nyoman Kamasan
Jurnal Kefarmasian Indonesia VOLUME 12, NOMOR 2, AGUSTUS 2022
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v0i0.5641

Abstract

AVaginal atrophy is one of the most common health problems in postmenopausal women. The disease is characterized by decreasing layers and maturation index of the vaginal epithelium and an increase in vaginal pH. A decrease in vaginal acidity will increase the susceptibility to infections since acidic pH protects the vagina from pathogens infection. The previous study has proven that phytoestrogens were effective to improve the microstructures of the vaginal, reduce urogenital complaints, and improve vaginal pH. The purple sweet potato (Ipomea batatas L.) which is cultivated in Bali was recognized for its high anthocyanin content and was reported to have phytoestrogen activity. The aim of the present study is to evaluate the effect of purple sweet potato on vaginal pH in an animal model of menopause. The randomized pre-test post-test control group design study was conducted using 36 bilateral ovariectomized female Wistar rats as an animal model of menopause. The animals were divided randomly into a control group (T0) which was given saline and a treatment group (T1) given an ethanolic extract of purple sweet potato 400 mg/day for 30 days. Vaginal pH was measured before treatment, every week during treatment, and after treatment. The result of the study showed that there was an increase in vaginal pH of the T0 on the 2nd and 3rd week, and after treatment (P
Potensi Senyawa Fitokimia pada Tumbuhan dalam Menurunkan Kadar Glukosa Darah pada Diabetes Melitus: Potential of Phytochemical Compounds in Plants in Lowering Blood Glucose Levels in Diabetes Raymond Elbert Budianto; Ni Made Linawati; I Gusti Kamasan Nyoman Arijana; Ida Ayu Ika Wahyuniari; I Gusti Nyoman Sri Wiryawan
Jurnal Sains dan Kesehatan Vol. 4 No. 5 (2022): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v4i5.1259

Abstract

Diabetes melitus adalah gangguan metabolik kronis yang ditandai dengan hiperglikemia atau tingginya glukosa dalam darah karena gangguan dalam sekresi maupun respons terhadap hormon insulin. Menurut data dari International Diabetes Federation, prevalensi diabetes melitus di seluruh dunia sudah sangat tinggi, yaitu 537 juta pada tahun 2021. Akan tetapi, sudah ada berbagai obat alternatif alami yang dapat digunakan untuk menangani penyakit tersebut. Dalam beberapa penelitian, banyak tanaman yang sudah teruji khasiatnya sebagai obat antidiabetes alami karena kandungan fitokimianya. Studi literature review ini dilakukan dengan pencarian data melalui Google Scholar dan Science Direct dalam kurun  waktu 10 tahun terakhir (2013-2022). Dari 29 artikel yang sesuai diperoleh hasil bahwa beberapa kandungan fitokimia pada tumbuhan seperti alkaloid dengan kemampuannya memperbaiki sel beta pankreas, flavonoid sebagai astringen, saponin dengan kemampuannya menstimulasi sekresi insulin dari sel beta pankreas, dan tanin sebagai antioksidan alami berpotensi menurunkan glukosa darah pada diabetes melitus tipe 1 dan 2.
Inflammation Marker and Leukocyte Parameter Ratio on Erythropoiesis Activity of Pediatric Acute Lymphoblastic Leukemia Wande, I Nyoman; Linawati, Ni Made; Dewi, Pande Putu Ayu Patria; Ariawati, Ketut
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 3 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i3.2395

Abstract

Acute leukemia is the most common malignancy found in children. A Complete Blood Count (CBC) examination can calculate the lymphocyte-to-leukocyte ratio, neutrophil-to-leukocyte ratio, and neutrophil-to-lymphocyte ratio to monitor the progress of Acute Lymphoblastic Leukemia (ALL). C-reactive protein is a marker of inflammation and can determine disease progression during treatment. The study's analytical design was observational, with a cross-sectional design conducted at the laboratory of the Clinical Pathology Faculty of Medicine, University of Udayana, and Ngoerah Hospital from March to August 2020. The study included 48 subjects, divided into three phases: induction, consolidation, and maintenance, in ALL patients. Specimen collection was collected in 2 tubes. Kolmogorov-Smirnov statistical analysis was used to assess normality, and homogeneity was evaluated with Levene’s test. The difference test was performed using the Kruskal-Wallis test. This study aimed to determine the relationship between parameters was performed with Spearman correlation. This study showed a strong correlation between hemoglobin levels and neutrophil/lymphocyte ratio (r= 0.358, p=0.015), hemoglobin and lymphocyte/leukocyte ratio (r= -0.287, p=0.048), hemoglobin and CRP (r= -0.493, p=0.000). Significant correlation between reticulocyte percentage and neutrophil/lymphocyte ratio (r= 0.505, p=0.000), neutrophil/lymphocyte ratio and CRP (r= -0.870, p=0.000), neutrophil/leukocyte ratio and lymphocyte/leukocyte ratio (r= 0.960, p=0.000). Significant correlations have been found between hemoglobin levels and the neutrophil/lymphocyte ratio (NLR), which is a measure of the lymphocyte/leukocyte ratio (LLeu-ratio), along with CRP. This relationship has been connected to inflammation in ALL patients following chemotherapy. CBC is an easy-to-use test to determine whether inflammation is present in ALL patients following chemotherapy.
Analisis Bibliometrik : Mekanisme Aksi dan Efektifitas Flavonoid Terhadap Gagal Ginjal Mathar, Muhammad Ashhabul Kahfi; Linawati, Ni Made; Wahyuniari, Ida Ayu Ika; Widianti, I Gusti Ayu
Jurnal Ilmu Kedokteran Vol 19, No 1 (2025): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v19i1.2025.19-28

Abstract

Flavonoids have strong antioxidant properties, which can reduce cell damage that can worsen kidney function. By reducing cellular oxidation, flavonoids help protect the kidneys from damage and inflammation that can lead to kidney failure. In addition, flavonoids also have anti-inflammatory properties to reduce chronic inflammation in the kidneys. Inflammation plays a key role in diseases such as kidney injury, diabetes, asthma, cardiovascular disease and cancer. Diet can affect various stages of inflammation and can have a significant impact on several inflammatory diseases. Increasing scientific evidence suggests that polyphenolic compounds, such as flavonoids, found in fruits, vegetables, nuts, or chocolate, can have anti-inflammatory properties. This reason clearly proves the importance of flavonoid antioxidant activity in inflammation therapy. The method used in this literature review is a bibliometric analysis by searching data through the PubMed database, and Google Scholar and Scopus. The search results of 10 appropriate journals, found that flavonoid compounds can inhibit regulatory enzymes or transcription factors that are important for controlling mediators involved in inflammation. 
Light physical activity and vitamin D improve sperm quality of male Rattus norvegicus exposed to cigarette smoke Amaral, Meriana Barreto; Satriyasa, Bagus Komang; Yasa, I Wayan Putu Sutirta; Sudarmaja, I Made; Jawi, I Made; Manuaba, Ida Bagus Putra; Linawati, Ni Made; Widiyanti, I Gusti Ayu
Physical Therapy Journal of Indonesia Vol. 6 No. 1 (2025): January-June 2025
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v6i1.261

Abstract

Background: Male infertility is a significant global health issue, with rising prevalence linked to environmental factors such as secondhand smoke, which impairs sperm quality. This study aimed to examine how light physical activity and vitamin D supplementation improved sperm quality in rats exposed to cigarette smoke. Methods: This study used a randomized experimental design with 38 rats, divided into two groups. The control group was exposed to cigarette smoke and received vitamin D at 18 IU/kg body weight (BW) daily. The treatment group was exposed to cigarette smoke, received the same vitamin D dose, and underwent light physical activity. The 35-day research period involved the analysis of evaluated parameters. Differences between these parameters were assessed using an independent t-test, with statistical significance set at a P-value < 0.05. Results: Compared to the control group (p-values< 0.001), the treatment group, receiving light physical activity and vitamin D supplementation, exhibited significantly lower malondialdehyde (MDA) levels (0.96 ± 0.14 nmol/mL), higher vitamin D receptor expression (2.44 ± 0.58 cells/HPF), and increased testosterone levels (4.33 ± 0.76 ng/dL). Sperm quality was also significantly improved in the treatment group, showing higher sperm count (2.24 ± 0.81 × 10⁶/mL), motility (4.20 ± 0.23%), viability (3.07 ± 0.39%), and normal morphology (5.34 ± 0.17%) (p-values< 0.001). Conclusion: Light physical activity and vitamin D supplementation improved sperm quality in cigarette smoke-exposed male rats, correlating with reduced MDA and elevated vitamin D receptor expression and testosterone. Further clinical trials are warranted to explore human applications.
Review Article: Histological Description of Hepars that Experience Fatty Liver Mulianti, Rizki; Linawati, Ni Made
Indonesian Journal of Global Health Research Vol 6 No 5 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6i5.3593

Abstract

The liver is one of the most vital organs and the largest gland, weighing approximately 1500 grams or 2.5% of the total adult body weight. One of the liver's functions is to metabolize nutrients such as fat, protein, and carbohydrates. Fats are synthesized from carbohydrates and proteins, mainly in the liver. Fat function as a source of energy provides essential fatty acids, and plays an important role in dissolving vitamins. A high-fat diet (HFD) is a diet that involves consuming fatty and high-cholesterol foods which can have the risk of causing an increase in lipid levels in the blood, known as hyperlipidemia. Excessive accumulation of fat in liver cells is called fatty liver. Histologically, Non-alcoholic Fatty Liver Disease (NAFLD) is categorized into two types, namely Non-alcoholic Fatty Liver (NAFL) which shows fatty liver without evidence of inflammation in the form of swelling of hepatocyte cells, and Non-alcoholic Steatohepatitis (NASH), namely the presence of fatty liver accompanied by inflammation in the form of swelling of hepatocyte cells, with or without fibrosis.
A Review on the Effect of Cell Phone Electromagnetic Radiation on Histological Testicles Farmananda, Irsandi Rizki; Farindra, Irmawan; Linawati, Ni Made
MAHESA : Malahayati Health Student Journal Vol 4, No 12 (2024): Volume 4 Nomor 12 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i12.15546

Abstract

ABSTRACT Cell phones have become an essential possession for individuals in today's society. Nevertheless, beneath their widespread use lies a potential hazard in the form of electromagnetic radiation. The level of radiation emitted varies, along with its impact on the human body, particularly on the reproductive system. This research aims to consolidate findings from existing studies on the influence of cell phone electromagnetic radiation on the histological makeup of the testes. A review of literature was conducted following a PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) approach, utilizing the PubMed and Scopus journal publication databases. A total of 133 articles were identified through the database search, with inclusion and exclusion criteria established based on the PICOS framework. Following the screening process, six articles met the predetermined criteria and were deemed suitable for analysis. The results of the study demonstrate that exposure to electromagnetic radiation led to alterations in the histological structure of the testes in experimental animals. In conclusion, prolonged exposure to electromagnetic radiation has an impact on the histological composition of the testes in experimental animals. Keywords: Electromagnetic Radiation, Histology, Testicles, Cell Phone
Co-Authors A.A.A. Biantari Apsari Trinaya ADNYANA PUTRA, I GEDE AGUS DIVA Adnyana, I Made Diatmika Agus Eka Darwinata Ali Djamhuri Amaral, Meriana Barreto Anak Agung Ngurah Subawa Arijana, I G Kamasan Nyoman Arijana, I Gusti Nyoman Kamasan Arijana, I.G Kamasan Nyoman Arijana, IGK Nyoman Bagus Komang Satriyasa Dakshayani Vijayan Dewa Agung Gina Andrini Dewa Ayu Agus Sri Laksemi Dewa Ayu Agus Sri Laksmi Dewa Putu Gede Purwa Samatra Dewi, Pande Putu Ayu Patria Etik Nurhidayati Eugenia Meidy Febrina, Jessica Fedik Abdul Ratam Gede Wirata Harinder Kaur I Dewa Gede Angga Triadi Nata I Dewa Made Agus Paramarta Putra I Gde Haryo Ganesha I Gusti Ayu Artini I Gusti Ayu Dewi Ratnayanthi I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan Arijana I Gusti Kamasan Nyoman Arijana I Gusti Kamasan Nyoman Arijana I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Gusti Nyoman Sri Wiryawan I Gusti Nyoman Sri Wiryawan I Ketut Tunas I Made Jawi I Made Krisna Dinata I MADE MULIARTA . I Made Niko Winaya I Made Sudarmaja I Made Sudarmaja I Nyoman Wande I Nyoman Wande I Putu Dema Prasetya I Wayan Juli Sumadi I Wayan Putu Sutirta Yasa I Wayan Rai Widarta I Wayan Sugiritama I Wayan Sugiritama I Wayan Sugiritama I Wayan Sumardika I.A.A. Widhiartini Ida Ayu Ika Wahyuniari Ida Bagus Putra Manuaba Intan Syahirah Bt Abdul Rauap Irmawan Farindra Irsandi Rizki Farmananda Jaspreet Kaur Juven Luvianto Ketut Ariawati Komang Ayu Trisnadewi Luh Made Indah Sri Handari Adiputra Luh Made Mas Rusyati Luh Putu Ratna Sundari Luvianto, Juven Muhammad Ashhabul Kahfi Mathar N. Adiputra Ni Eka Dewi Ambarawati Ni Eka Dewi Ambarawati Ni Kadek Ayu Satya Dewanti Ni Luh Gede Yoni Komalasari, Ni Luh Gede Yoni Ni Luh Rustini Ni Made Mertaniasih Ni Nyoman Sri Budayanti Ni Putu Candra Paramita Ni Wayan Arni Sardi Nuril Hidayah Nyoman Intan Cahaya Pertiwi Nyoman Pratiwi Hapsari Dewi Putri, Ni Putu Windi Sukma Putu Ayu Asri Damayanti Putu Ayu Sita Saraswati Putu Diah Pratiwi Radjibu, Gabriela Anastasya Viorezky Rastika, I Gede Agus Ratnayanthi, I Gusti Ayu Dewi Ratnayanti, Gusti Ayu Dewi Raymond Elbert Budianto Riezky Januar Pramitha Rizki Mulianti Ruthirar Kalaichelvam Sawitri, Anak Agung Sagung Sianny Herawati Sidauruk, Dona Uli Sugijanto - Sugijanto - Susy Purnawati Tjokorda Gede Oka Tohjiwa, G. Raditya Vania Lannisa Haestidyatami Wahyuddin, Wahyuddin Wicaksana, I Dewa Agung Gede Oka Dwi Widianti, I Gusti Ayu Widiyanti, I Gusti Ayu Wijaya, Debby Chesilya YASA, WAYAN PUTU SUTIRTA Yusuf Nasirudin