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Journal : Jurnal Natural

Bioassay on n-Hexane Extract of Leaves Cassia alata against Candida albicans Muhammad Bahi; Radilla Mutia; Mustanir Mustanir; Endang Lukitaningsih
Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Universitas Syiah Kuala

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Abstract

The study of biological activity from n-hexane extract of leaves’ Cassia alata had been done against Candida albicans as bioindicator. Dried leaves of C. alata (850 g) were macerated into n-hexane to obtain a dark green crude extract (31.79 g). The n-hexane crude extract was then subjected to vacuum liquid chromatography (VLC) and eluted with n-hexane and ethyl acetate to give 4 sub-fractions, namely fraction A (7.7251 g), fraction B (1.9419 g), fraction C (1.3565 g) and fraction D (1.4737 g) respectively. Based on phytochemical analysis, both nhexane crude extract and chromatographic sub-fractions contained steroids as their secondary metabolite constituent. The antifungal activity was tested against C. albicans using the agar-disc diffusion method with three different concentrations (10, 30 and 50%). Fraction D showed medium antifungal activity at 10.6, 11.3 and 12.6 of diameter-inhibition zones (mm) respectively.
ANALYSIS OF MACRONUTRIENCONTENT, GLYCEMIC INDEX AND CALCIUM OXALATE ELIMINATION IN Amorphophallus campanulatus (Roxb.) Endang Lukitaningsih; Rumiyati Rumiyati; Ika Puspitasari; Meti Christiana
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

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Abstract

Recently, the research to find alternative sources of carbohydrates as a replacement for rice has been developed. Walur is one of the carbohydrate sources that can be explored because it can be grown in any area with out special treatment. However, walur has limitation for direct consumption, because it contains calcium oxalate. The purposes of this study were to determine the chemical character (macronutrient content), calculate glycemic index and get the proper washing techniques to elimin ate calcium oxalate of walur. Macro nutrients content studied in this research include carbohydrates, fats, protein, star chand crude fiber. Analysis of macronutrients has been chemically done, while the glycemic index was measured by in vivo using glucose as a standard. Elimination of calcium oxalate was conducted by washing the fresh walur tubers using a solution of0.01NHCl-NaOH, 5% NaCl, and solution of lemon-lime. The content of oxalate before and after washing was analyzed by permanganometry method. The results showed that walur containing 4.34 ±0.07% of reducingsugar,3.24 ± 0.06 % of not-reducing sugar, 11.27±0.40 % of crude fiber,0.03±1.05 % of starchand0.57±0.01 % of protein. Qualitative analysis of fatty acids showed that hexade canoicacid, octade cadienoicacid, and the acide icosatetranoic were detected in high concentrations. The glycemic index valueof walur was relative lylow, about of 16.9. In addition, washing technique using a solution of lemon-lime was the most excellent technique and can reduce the oxalate content up to61.82%.Fromthis research, it can be concluded that walur can be used as food substitute esrice after washing treatment using lemon-lime solution to remove the calcium oxalate content.
Co-Authors -, Ariyanti -, Sutriyanto Abdul Rohman Abdul Rohman Aditya Wisnusaputra Aditya Wisnusaputra, Aditya Adjara, Syafia Mustika Ag. Yuswanto Ag. Yuswanto Agung Endro Nugroho Aisyah, Andi Nur Akhmad Kharis Nugroho Alfat Fadri Amalia Miranda Amalia, Dita Amanita K. Hana Amelia Sari Andaru Dahesihdewi Andi Nur Aisyah Annisa Novarina Ardian Widyatmoko Argandita Meiftasari Ari Sudarmanto Ari Sudarmanto Arief Nurrochmad Arifah, Mitsalina Fildzah Artania Adnin Tri Suma B. S. Ari Sudarmanto Burhan, Asril Bustomi, Tomy Bustomi, Tomy Daniel Happy Putra Devyanto Hadi Triutomo Dini Maharani Dirman, Arifin Dwi Hastutik Earlia, Nanda Edy Meiyanto Effendi, Fatiha Citra Erna Prasetya Ningrum Esti Dyah Utami Etty Sulistyowati Fawzy, Salsabila Yusfita Fita Rahmawati Hanum, Faridah Hayati, Rima Hilda Ismail, Hilda I Gede Agus Juniarka I Gede Agus Juniarka, I Gede Agus Ika Puspita Sari Ika Puspita Sari Ika Puspitasari Ika Puspitasari Indah Purwantini Indah Tri Nugraha Indah Tri Nur'aini Irfan Muris Setiawan Jauhari, Fahmi Ihsanuddin Khairunissa Irnanda Kurnianto, Rifki Wahyu La Ode Muhammad Andi Zulbayu Laeli Muntafi'ah Lina Primadesa, Lina Lisa Andina Lora Johana Tamba M, Suwaldi Marbun, Prajona Marlyn Dian Laksitorini Marwati Marwati Melania Perwitasari, Melania Meti Christiana Mohammad Fahrurrozi Mufrod, . Mufrod, . Muhammad Bahi Muhammad Bahi Muhammad Fahrurrozi Mustanir Mustanir Mutia Kusuma Wardani Nabila, Klarissa Nabilah, Nada Nanang Fakhrudin, Nanang Nining Sugihartini Noegrohati, Sri Pawestri, Sekar Ayu Pudjono Lukitaningsih Pudjono Pudjono Purwantiningsih Purwantiningsih Purwantiningsih Radilla Mutia Ragil S. Dianingati Rahim, Kurnia Rahmawati, Adillah Ratna Asmah Susidarti Ratna Asmah Susidarti Ratna Budhi Pebriana Ririn Suharsanti, Ririn Rizka Humardewayanti Asdie Rochmadi Lukitaningsih Rochmadi Rochmadi Ronny Martien Rumiyati Rumiyati Rumiyati Rumiyati Rumiyati Rumiyati Rumiyati, Rumiyati Rusydan, Azka Muhammad Ryan Radix Rahadhian, Muhammad Samsul Hadi Samsul Hadi Saputra, Lucky Octavianus Saputri, Revita Sekar Ayu Pawestri Siti Fatmawati Fatimah, Siti Fatmawati Siti Mufidatul Khasanah Sri Noegrohati Sri Noegrohati Standie Nagadi Subagus Wahyuono Subagus Wahyuono Sudarmanto, B.S. Ari Sudarmanto, Bambang Sulistyo Ari Sudibyo Martono Sugeng Riyanto Suwaldi . Suwaldi Martodihardjo Syamsu Nur Syamsu Nur, Syamsu Ulrike Holzgrabe Wardani, Ratih Kurnia Widayanti, Wasita Rachma Widyasari Putranti Wirasti, Wirasti Wiwik Supriati