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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Denny Widaya Lukman
Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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KARAKTERISTIK DAN PENGETAHUAN HIGIENE SANITASI PEDAGANG DAGING AYAM DI PASAR TRADISIONAL DI KOTA JAKARTA Hasudungan A Sidabalok; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.537 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2794

Abstract

Tujuan penelitian ini mengetahui karakteristik pedagang daging ayam (umur, tingkat pendidikan, pengalaman berjualan, pelatihan, dan statuskepegawaian) di pasar tradisional di Kota Jakarta dan hubungan antara karakteristik tersebut dengan pengetahuan terkait higiene dan sanitasiketika berjualan daging ayam. Sebanyak 217 pedagang daging ayam di pasar tradisional di DKI Jakarta digunakan sebagai responden. Metodepenentuan sampel dengan cluster random sampling dan penentuan sampel pasar dengan probability proportional to size (PPS). Data dianalisissecara deskriptif dan hubungan antara peubah diuji dengan uji Gamma. Hasil yang diperoleh adalah hampir seluruh responden (n= 216; 99,5%)berumur di atas 21 tahun, sebagian besar responden (n= 105: 52,3%) lulus SMP dan SMA, dan kebanyakan responden memiliki pengalamanberjualan 3 tahun. Tingkat pengetahuan responden termasuk dalam kategori sedang sampai dengan baik (n= 209; 96,3%). Hasil analisis datamenunjukkan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) dengan kekuatan korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) dengan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) mempunyai korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) mempunyai korelasi lemah (r= 0,296).
5. Analysis of thigh Death Chicken by Shear Values and Impedance Approach Razali Razali; Denny Widaya Lukman
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.698 KB) | DOI: 10.21157/ijtvbr.v1i2.6687

Abstract

Experiments were conducted to determine whether thigh meat from death chicken can be identified through Warner-Bratzler (WB) shear value and using the impedance value.  Thirty of thigh meat (Biceps femoris) were obtained from commercial slaughtering house classified into three groups namely halal slaughtered healthy chicken (AHS), slaughtered death chicken (AMS), and slaughtered stressed chicken (ALS).  Biceps femoris muscles were used to microscopic procedures and to assess WB score values and impedance values. This study showed that shear values of AMS thigh meat were lower than AHS and ALS after 5 and 9 h PM. This study indicated that the impedance value of AMS were significantly lower (P0.05) than AHS and ALS, it can be used to distinguish between halal thigh and the thigh meat from slaughtered death chicken.
Keberadaan Escherichia coli Extended Spectrum ?-lactamase Resistan Antibiotik di Peternakan Sapi Perah Cijeruk, Bogor Herwin Pisestyani; Denny Widaya Lukman; Hadri Latif; Mirnawati Sudarwanto
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.505

Abstract

Contamination of Extended spectrum-betha lactamase (ESBL) producing Escherichia coli in milk can cause public health problems. The misuse of antibiotics in dairy has implications for the emergence of multi-resistant bacteria that can infect humans through food (foodborne diseases). The ESBL producing E. coli is resistant to ?-lactam antibiotics (including penicillins and 3rd and 4th derivatives of cephalosporins). This study aimed to analyze the phenotype of antibioticresistant ESBL-producing E. coli from livestock, farmer, dairy cows, milk, and pasteurized milk. The research was conducted on a dairy farm in Cijeruk District, Bogor. The method used was the ESBL EC tricycle for phenotype confirmation consisting of culture on tryptone bile x-glucuronide (TBX) agar and MacConkey agar (MCA) with the addition of the cefotaxime antibiotic 4 µg/mL for the presumption of ESBL-producing E. coli followed by confirmation of ESBL producing E. coli with indol biochemical tests with sulfite indole motility (SIM) and double disk diffusion test (DDST) on Mueller Hinton agar (MHA). The results from this study showed that ESBL producing E. coli was not found in water, effluent, milker hand swabs, teat swabs, and milk from the individual cows. ESBL producing E. coli was identified from fresh milk (2/10) and pasteurized milk (2/10) on the same farm. ESBL-producing E. coli were resistant to eight antibiotics (ampicillin, cefotaxime, ceftazidime, cefpodoxime, cephalothin, streptomycin, azithromicyn, enrofloxacin). Multidrugresistant ESBL-producing E. coli is one of the foodborne diseases that pose a health threat to the community.
Analisis Kadar Nitrit pada Sarang Burung Walet Asal Pulau Sumatera Menggunakan Metode Kromameter Platika Widiyani; Mirnawati B Sudarwanto; Hadri Latif; Denny Widaya Lukman
Acta VETERINARIA Indonesiana Vol. 11 No. 2 (2023): Juli 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.2.148-155

Abstract

Kadar nitrit dalam sarang burung walet (SBW) telah menjadi perhatian dalam beberapa tahun terakhir. SBW yang diekspor dari Indonesia ke Negara Tiongkok harus memenuhi standar kadar nitrit (NO2), yaitu maksimum 30 ppm. Dinamika perkembangan teknologi dan jaman saat ini menuntut instrumen pengujian kadar nitrit secara akurat, diantaranya menggunakan spektrofotometer dan kromameter. Penelitian ini mengkaji kadar nitrit pada SBW bersih yang telah dilakukan pencucian asal Pulau Sumatera dengan menggunakan metode spektrofotometer dan mengevaluasi warna menggunakan kromameter berbasis sistem CIE pada parameter L*, a*, b*, C*, dan h*. Jumlah sampel ditentukan secara purposif dari rumah burung walet (RBW). Sebanyak 18 sampel SBW berasal dari berbagai wilayah di Sumatera. Sampel SBW diuji kadar nitritnya menggunakan spektrofotometer di Balai Besar Uji Standar Karantina Pertanian (BBUSKP) Jakarta dan kromameter diuji di laboratorium Ilmu Teknologi Pangan IPB, Bogor. Hasil kadar nitrit pada SBW menunjukkan bahwa persentase kadar nitrit di bawah 30 ppm adalah 72,22%. Nilai rata-rata L* pada grup A (kadar nitrit >30 ppm) dan B (kadar nitrit <30 ppm) secara berurutan adalah sebesar 67,65±1,97 dan 68,47±5,25. Hasil analisis statistik dengan uji-t menunjukkan tidak terdapat perbedaan yang signifikan (p>0,05) antara nilai L*, a*, b*, C* dan *h pada kedua grup. Metode kromameter tidak dapat digunakan sebagai metode tunggal dalam mengukur kadar nitrit pada SBW serta tidak dapat membedakan secara signifikan warna SBW yang berasal dari RBW yang berbeda.
Penilaian Pendedahan Kualitatif Virus Penyakit Mulut dan Kuku melalui Pemasukan Kulit Sapi Mentah Garaman dari Malaysia ke Indonesia di Pelabuhan Tanjung Priok, Indonesia Basri, Chaerul; Sekarsana, Disty Ayu; Lukman, Denny Widaya
Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.1.65-75

Abstract

Penyakit mulut dan kuku (PMK) merupakan penyakit yang menjadi perhatian semua negara karena dapat menimbulkan kerugian ekonomi yang besar. Penyakit ini sangat mudah menyebar antar negara atau wilayah sehingga dikategorikan sebagai transboundary animal disease. Salah satu penyebab penyebaran PMK adalah perdagangan hewan dan produk hewan antar negara atau wilayah. Lalu lintas kulit sapi mentah dapat membawa risiko penyebaran PMK. Penelitian ini bertujuan untuk melakukan penilaian risiko pendedahan kualitatif terhadap masuk dan tersebarnya PMK dari kulit sapi mentah garaman ke Indonesia. Penilaian ini dilaksanakan dengan mengembangkan biological pathway terhadap likelihood pendedahan agen penyakit melalui kulit sapi mentah garaman dari negara asal Malaysia ke Indonesia. Tingkat likelihood (kemungkinan) kualitatif pada penilaian pendedahan (exposure assessment) didasarkan pada Biosecurity Import Risk Analysis Guidelines 2016, sedangkan tingkat ketidakpastian ditentukan berdasarkan pedoman yang diberikan oleh The European Food Safety Authority (EFSA). Penilaian akhir pendedahan virus PMK melalui kulit sapi mentah garaman dari Malaysia di Pelabuhan Tanjung Priok menunjukkan tingkat likelihood sedang, dengan tingkat ketidakpastian yang rendah. Tingkat likelihood sedang mengindikasikan bahwa peluang kemungkinan kejadian tersebarnya virus PMK melalui kulit sapi mentah garaman dari Malaysia memiliki peluang yang sama besar yaitu antara terbawa atau tidak terbawanya virus PMK melalui kulit sapi mentah garaman dari Malaysia. Penerapan biosekuriti sebagai tindakan mitigasi untuk mengurangi risiko penyebaran virus PMK perlu diterapkan pada tempat kedatangan kulit sapi mentah garaman dan di gudang penyimpanan kulit.
Sanitation and Hygienic Practices of Ready-to-Drink Milk Seller Based on Total of Coliform and Staphylococcus aureus Pisestyani, Herwin; Ramadhani, Nada Nursaffana; Sudarwanto, Mirnawati; Lukman, Denny Widaya; Wicaksono, Ardilasunu
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.14-22

Abstract

Dairy products are innovative to improve the economic value of milk. Poor attention to the importance of good hygiene and milk handling of milk can lead to microbial contamination of milk, such as coliform and Staphylococcus aureus. This study aimed were to define the safety of ready-to-drink milk which sold around Dramaga and Cilibende IPB University campuses based on the total coliform and S. aureus and to determine the factors that affected it. Data were obtained by questionnaire from 12 sellers, furthermore milk samples were taken from 13 ready-to-drink milk's sellers with 3 replication The data were presented descriptively and analyzed using chi-square test to determine the relationship between the practice of the sellers with total contamination of coliform and S. aureus. The data results were compared with SNI 01-7388-2009 concerning the Maximum Limits of Microbial Contamination in Food. The average of coliform contamination in Dramaga and Cilibende 5,92í—103 ± 9,28í—103 CFU/ml and 7,60í—104 ± 1,25í—105 CFU/ml, respectively. The average of S. aureus contamination in Dramaga and Cilibende 3,84í—102 CFU/ml ± 5,36í—102 and 8,44í—104 ± 1,96í—105 CFU/ml, respectively. Furthermore, several practices of ready-to-drink milk sellers had a significant relationship with coliform contamination (p<0.05), which were the table cleanliness (p=0.020), distance to the crowd (p=0.001), and hand washing (p=0.001). The practice of ready-to-drink milk sellers had a significant relationship with S. aureus contamination (p<0.05) which were the table cleanliness (p=0.020), and distance of the crowd (p=0.020). The high contamination by coliform and S. aureus is caused by the poor sanitation and personal hygiene of the milk seller during the serving and processing of the milk.
An Evaluation of Draminski Detector as an Early Detection Tool for Subclinical Mastitis in Dairy Cattle in Pondok Ranggon Farm Pisestyani, Herwin; Permana, Indra; Basri, Chaerul; Lukman, Denny Widaya; Sudarwanto, Mirnawati
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.6-14

Abstract

Subclinical mastitis is one of the main problems in the development of dairy farming in Indonesia. Several screening test tools have been developed to support of control program for this problem. This study aimed to assess the suitability of the Draminski detector as a screening tool for subclinical mastitis in dairy cows. Quaternary milk samples from 27 dairy cows during normal lactation in the Pondok Ranggon area, East Jakarta were collected to be evaluated for the possibility of coming from quarters suffering from subclinical mastitis. Each milk sample was tested with 3 types of tests, i.e. the Breed method (golden standard) which was based on the number of somatic cells, the device Milkchecker which was based on electrical conductivity, and the Draminski detector which was based on electrical resistance. The results showed that the Draminski detector had a poor level of compatibility with the Breed method as the golden standard (Kappa value 0,013) and the Milkchecker tool (Kappa value 0,036). This test method was also considered to have poor test performance in diagnosing cases of subclinical mastitis in dairy cattle with a sensitivity value of 3,5%, specificity 100%, positive predictive value 100%, negative predictive value 18,8%, estimated prevalence 2,9%, and the true prevalence was 81,7%. In conclusion, the Draminski detector cannot be used to replace the Breed method and Milkchchecker as a screening test tool in cases of subclinical mastitis in dairy farms in Indonesia which were generally reared in the dairy farms.
Detection of Foot and Mouth Disease Virus in Salted Raw Cowhide from Malaysia in Tanjung Priok Port, Indonesia Sekarsana, Disty Ayu; Basri, Chaerul; Lukman, Denny Widaya
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.48-56

Abstract

Animal products such as raw salted cowhide are thought to have the potential to transmit the foot and mouth disease (FMD) virus from the infected zone. Indonesia imports raw salted cowhide from Malaysia, so it has the potential to transmit FMD to Indonesia which enters through Tanjung Priok Port. This study aimed to investigate the presence of the FMD virus in raw salted cowhide from Malaysia. The number of samples was collected from each container of raw salted cowhide imported through Tanjung Priok Port during August–December 2022. A total of 21 samples were obtained from 21 bulk containers containing raw salted cowhide. Real time q Polymerase Chain Reaction (RT-qPCR) was used to investigate foot and mouth disease virus in samples. The RT-qPCR screening test on 21 samples reported that salted raw cowhide was free from the FMD virus. Continuous monitoring and surveillance protocols for salted rawhide imported from non-free countries need to be carried out at other points of entry.
Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Economic Impact of Foot and Mouth Disease in Dairy Farmers at Koperasi Peternakan Bandung Selatan (KPBS) Pangalengan, Indonesia Taufiqqurrahman, Mas; Sudarnika, Etih; Lukman, Denny Widaya
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103474

Abstract

This study aims to analyze the economic impact of the foot and mouth disease (FMD) outbreak on dairy farmers who are members of the Pangalengan South Bandung Livestock Cooperative (KPBS). Data were collected through structured questionnaires, in-depth interviews, and field observations of 100 FMD-affected farmers during September-November 2023, with follow-up evaluation one year after the outbreak. The results showed that during the outbreak (29 days), the average loss per farmer reached IDR 44,018,068 or IDR 6,288,295 per head, mostly due to decreased milk production, dairy cow mortality, and disease control costs, including treatment, vaccination, and disinfection. One year after the outbreak (365 days), economic losses were IDR 46,077,090 or IDR 6,582,441 per head, dominated by the residual impact of FMD in the form of a 39.75% reduction in milk yield (18.31 kg/day). Economic losses also involved additional costs, such as vector control and livestock burial, which further burdened farmers. These findings highlight the importance of more effective FMD control policies, including mass vaccination, improved biosecurity and financial assistance for affected farmers, to reduce economic impacts and support long-term recovery.
Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Afiff , Usamah Agus Wijaya Agustin Indrawati Ahmad Nurhakim Aminudin, Muhammad Rizki Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Ekowati Handharyani Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Indra Permana, Indra Inriyanti Assa Irena Ivania Kallau, Novalino Harold Geoffrey Kamil Riski Sidik koekoeh santoso Kumala Andri Asari Loisa Loisa Loisa, Loisa Manuel da Costa Maya Shofa Milawarni Milawarni Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Naoaki Misawa Nararya Adinata Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putra Wijaya, Erlangga Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Ramadhani, Nada Nursaffana Razali Razali Rinaldi Ghurafa Rismayani Saridewi Rismayani Saridewi, Rismayani Risqika Akla Velayati Rochman Naim Ronald Tarigan Roostita L Balia Rotinsulu, Dordia Anindita Saukhan, Falih Prenata Sekarsana, Disty Ayu Siti Gusti Ningrum sri murtini . Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Widagdo Sri Nugroho Woro Wulandari Kalanjati Wyanda Arnafia Yusuf Ridwan