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Monitoring Penggunaan Antibiotik dengan Metode ATC/DDD dan DU90% di RSUD Abepura Jayapura, Indonesia Hasrianna Hasrianna; Nurul Annisa; Tiana Milanda; Ivan S. Pradipta; Rizky Abdulah
Indonesian Journal of Clinical Pharmacy Vol 4, No 3 (2015)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2448.733 KB) | DOI: 10.15416/ijcp.2015.4.3.218

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Tingginya penggunaan antibiotik akan meningkatkan potensi penggunaannya yang tidak rasional dan berdamPak pada tingkat mortalitas, biaya, dan resistensi khususnya dalam lingkungan rumah sakit. Studi observasi dengan data retrospektif telah dilakukan untuk mengevaluasi penggunaan antibiotik dari April 2013–Maret 2014 menggunakan metode ATC/DDD dan DU 90%. Hasil penelitian menunjukkan antibiotik yang masuk ke dalam segmen DU 90% pada periode I adalah kotrikmoksazol 480 mg tablet(40,34 DDD/kunjungan) dan amoksisilin 500 mg tablet (4,53 DDD/kunjungan), periode II adalah sefiksim sirup kering (0,68 DDD/kunjungan), amoksisilin 500 mg tablet (0,41 DDD/kunjungan), siproflokasain 500 mg tablet (0,31 DDD/kunjungan), doksisiklin 100 mg (0,26 DDD/kunjungan), sefiksim 100 mg kapsul (0,15 DDD/kunjungan), sefadroksil 500 mg kapsul (0,12 DDD/kunjungan),seftriakson 1 gr injeksi (0,08 DDD/kunjungan), dan periode III adalah kotrimoksazol 480 mg tablet (74,85 DDD/kunjungan). Tingginya penggunaan antibiotik setiap kunjungan pada penggunaan kotrimoksasol merupakan sebuah tanda ketidakrasionalan dalam penggunaan antibiotik. Diperlukan studi kualititaf untuk mengetahui pola ketidakrasionalan dalam penggunaan antibiotik pada rumah sakit tersebut danmengembangkan model intervensi yang tepat.Kata kunci: Antibiotik, ATC/DDD, DU 90%, rumah sakitMonitoring Use of Antibiotics with ATC/DDD and DU90% Method in Abepura Hospital Jayapura, IndonesiaThe high use of antibiotics will increase its irrational use, affect the mortality rates, costs and resistance, especially in a hospital. We conducted an observational study with retrospective data to evaluate the use of antibiotics from April 2013–March 2014 using the ATC/DDD and DU90% methods. The results showed the antibiotic included DU 90% segment in the first 4 months period were cotrimoxazole tablets 480 mg (40.34 DDD per encounter) and amoxicillin tablets 500 mg (4.53 DDD per encounter), in the second period were cefixime dry syrup (0.68 DDD per encounter), amoxicillin tablets 500 mg (0.41 DDD per encounter), ciprofloxacin tablets 500 mg (0.31 DDD per encounter), doxycycline tablets 100 mg (0.26 DDD per encounter), cefixime tablets 100 mg capsules (0.15 DDD per encounter), cefadroxil tablets 500 mg capsule (0.12 DDD per encounter), ceftriaxone injection 1 g (0.08 DDD per encounter), and during the third period was cotrimoxazole tablets 480 mg (74.85 DDD per encounter). The data showed that cotrimoxazole has the highest rate of utilization per visit which is a signal for irrational use. Qualitative study is needed to describe irrational use of antibiotics in the hospital and to find the appropriate intervention model.Key words: Antibiotics, ATC/DDD, DU 90%, hospital
Efek Ekstrak Angkak dalam Menghambat Proliferasi Sel Kanker Prostat dan Payudara Vesara A. Gatera; Tiana Milanda
Indonesian Journal of Clinical Pharmacy Vol 10, No 2 (2021)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/ijcp.2021.10.2.119

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Kanker payudara dan prostat masih menjadi kasus prevalensi tertinggi hingga saat ini, untuk itu diperlukan beberapa alternatif terapi yang berasal dari bahan alam dengan efek samping yang relatif rendah. Salah satu bahan alam yang memiliki aktivitas sitotoksisitas adalah angkak yang merupakan hasil fermentasi dari jamur Monascus pada medium beras, namun potensinya belum optimal diteliti terutama pada kanker payudara dan prostat. Penelitian ini bertujuan untuk menguji sitotoksisitas ekstrak etanol, etil asetat, dan n-heksan dari angkak terhadap lini sel kanker prostat DU145, lini sel kanker payudara MDA-MB-231 dan menentukan golongan senyawa yang diduga memiliki aktivitas sitotoksik. Penelitian ini menggunakan metode 3-4,5-di-methylthiazol-2-y1-5-(3-carboxymethoxyphenyl)-2-(sulfophenyl)-2H-tetrazolium (MTS). Berdasarkan hasil penelitian, diketahui bahwa ekstrak n-heksan memiliki sitotoksisitas terbaik dengan nilai IC50 138,900 μg/mL pada lini sel DU145 dan 192,623 μg/mL pada lini sel MDA-MB-231, sedangkan pada ekstrak etanol dan etil asetat memiliki tingkat sitotoksik yang rendah dengan masing-masing nilai IC50 yaitu 649,426 μg/mL pada lini sel DU145, di luar rentang konsentrasi uji pada sel lini MDA-MB-231 dan 207,916 μg/mL pada lini sel DU145 dan 259,408 μg/mL pada lini sel MDA-MB-231. Disimpulkan bahwa ekstrak n-heksan angkak memiliki aktivitas sitotoksisitas terbaik, kemampuan tersebut diduga berasal dari senyawa flavonoid dan kuinon.Kata kunci: Angkak, kanker prostat, Monascus, sitotoksisitas The Effect of Angkak Extract in Inhibiting Cell Proliferation of Prostate and Breast CancerAbstractProstate cancer attacks the prostate gland in the male reproductive system. However, angkak, a product of fermentation from Monascus mushrooms on rice medium, exhibits potent anticancer activity. This study, therefore, aims to examine the cytotoxicity of ethanol, ethyl, and n-hexane extracts from angkak against DU145 prostate, as well as MDA-MB-231 breast cancer cells line, and determine the class of compounds with cytotoxic activity. The cytotoxic assay was performed using the 3-4,5-di-methylthiazol-2-y1)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. According to the results,  the n-hexane extract of angkak exhibited the most impressive cytotoxicity at IC50 138,900 μg/mL in DU145 cell, and 192,623 μg/mL, in MDA-MB-231 cell line. Meanwhile, the ethanol and ethyl acetate extracts exhibited low cytotoxic levels with  IC50 649,426 μg/mL and 207,916 μg/mL, respectively, in DU145 cell line, as well as unidentified results and 259,408 μg/mL, respectively, in MDA-MB-231 cell line. Therefore, angkak n-hexane extract was concluded to exhibit the best cytotoxicity activity, with the potential compounds having this activity derived from flavonoid and quinone compounds.      Keywords: Angkak, prostate cancer, Monascus, cytotoxicity
Deteksi Gen Resistensi Ampisilin (bla) pada Escherichia coli Isolat Klinik dengan Metode Polymerase Chain Reaction Tiana Milanda; Bonar C. Saragih; Sri A. F. Kusuma
Indonesian Journal of Clinical Pharmacy Vol 3, No 3 (2014)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7852.854 KB) | DOI: 10.15416/ijcp.2014.3.3.98

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Escherichia coli merupakan bakteri batang Gram negatif yang dapat menjadi patogen jika jumlahnya meningkat atau berada di luar saluran pencernaan. E. coli yang patogen akan menghasilkan enterotoksin yang menyebabkan diare atau infeksi pada saluran kemih. Ampisilin merupakan salah satu antibiotik pilihan untuk mengatasi penyakit infeksi tersebut. Akhir-akhir ini ampisilin tidak lagi digunakan sebagai obat pilihan karena banyaknya kasus resistensi E. coli terhadap antibiotik tersebut. Penelitian ini bertujuanuntuk mendeteksi keberadaan gen yang bertanggung jawab terhadap resistensi antibiotik ampisilin pada E. coli isolat klinik. Sampel yang digunakan adalah hasil isolasi urin midstream pasien dengan gejala sistitis di Rumah Sakit Hasan Sadikin (RSHS) Bandung. Uji resistensi antibiotik menggunakan metode Polymerase Chain Reaction (PCR), baik PCR-koloni maupun PCR-DNA. Berdasarkan hasil uji resistensi terhadap ampisilin, E. coli hasil isolasi telah resisten terhadap ampisilin. Elektroforesis hasil PCR-koloni dan PCR-DNA menunjukkan bahwa resistensi terhadap ampisilin disebabkan oleh gen bla berukuran 199 pb. Diperlukan pemilihan antibiotik yang selektif dan rasional untuk mencegah resistensi ampisilin pada pasien dengan gejala sistitis.Kata kunci: bla, Escherichia coli, gen resistensi ampisilin, polymerase chain reactionDetection of Ampicillin Resistance Genes (bla) in Clinical Isolates ofEscherichia coli with Polymerase Chain Reaction MethodEscherichia coli is a rod negative Gram which could be pathogenic, if its value increases or located in outer gastrointestinal tract. Pathogenic E. coli will produce enterotoxin which will cause diarrhea or infection in urine tract. Ampicilin was one of particular antibiotics to overcome infection. Ampicilinnowadays is no longer used as first choice medicine, because of its resistance case. The aim of this research was to detect the presence of gene which is responsible to ampicilin resistant E. coli. We used isolated midstream urine from cystitis object in Hasan Sadikin Hospital as samples. Polymerase Chain Reaction (PCR) method (colony-PCR and DNA-PCR) were performed to invenstigate the antibiotic resistency. Based on the result of antibiotic susceptibility testing to ampicillin, E. coli samples were resistant to ampicilin. Electropherogram products of colony-PCR and DNA-PCR showed that the resistance case of ampicilin caused by bla gene (199 bp). Our result suggested that bla gene may be use to detect the ampicilin resistance. Furthermore, selective and rational antibiotic treatment is required toprevent ampicillin resistance in patients with symptoms of cystitis.Key words: Ampicillin resistance gene, bla, Escherichia coli, polymerase chain reaction
Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung TIANA MILANDA; MARLIA SINGGIH WIBOWO; TUTUS GUSDINAR; HARYANTO DHANUTIRTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.286 KB) | DOI: 10.5454/mi.1.1.5

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Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 min). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F001 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing the growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.
Modulation of Caspase-3 Expression by Arcangelisia flava Post Acetaminophen-Induced Hepatotoxicity in Rat’s Liver Steffi Liem; Tina Rostinawati; Ronny Lesmana; Sri Adi Sumiwi; Tiana Milanda; Mutakin Mutakin; Irma Melyani Puspitasari; Jutti Levita
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.412

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BACKGROUND: Acetaminophen, when used at low doses is a safe drug, but at higher doses it induces apoptosis in hepatoma cells. Arcangelisia flava that grows widely in Kalimantan Island, Indonesia, contains berberine which is effective in protecting the liver. This work was aimed to study the effect of A. flava extract on the modulation of caspase-3 in acetaminophen-induced hepatotoxicity.METHODS: Thirty-five Wistar male rats were divided into groups: I the normal control (water); II the negative control (Arabic gum powder or PGA, 2% in suspension); III the positive control (silymarin); IV-VII (A. flava extract 100, 200, 400, and 800 mg/Kg of body weight (BW), respectively) for 14 days. At day 15th, group II-VII were induced with acetaminophen 1000 mg/Kg of BW per oral for 7 days along with the extracts. At day 22nd, the animals were measured for their serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and gamma-glutamyl transferase (GGT), histological examination, and Western blotting.RESULTS: Acetaminophen elevated the SGOT and SGPT (3x compared to normal group), and GGT (5x compared to normal group) of the animals in group II. Pre-treatment with higher doses of A. flava extract (group VI and VII) significantly prevented the biochemical changes induced by acetaminophen. Normal histology of the liver was showed by group I, III, VII, whereas dilated sinusoids, central vein (CV) lesion, and local haemorrage were observed in group II, IV, V and VI. Western blotting showed an inhibition of caspase-3 expression by A. flava extract in dose-dependent manner.CONCLUSION: A. Higher dose A. flava extract shows hepatoprotective activity by preventing the elevation of serum transaminases and transferase levels. Eventually, no damage in the acetaminophen-induced rat’s liver was observed. This plant modulates the expression of caspase 3 protein in dose-dependent manner.KEYWORDS: Arcangelisia sp, caspase-3, berberine, hepatoprotective activity, NSAIDs, yellow root
POTENSI SAMBILOTO SEBAGAI OBAT ANTIDIABETES BERBASIS HERBAL Syah Akbarul Adha; Raden Maya Febriyanti; Tiana Milanda
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 4 No 1 (2019)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.736 KB) | DOI: 10.37874/ms.v4i1.118

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Penggunaan tanaman herbal atau obat tradisional oleh manusia telah tercatat selama ribuan tahun dan merupakan bagian dari sejarah dan kebudayaan yang masih ada sampai sekarang. Indonesia merupakan negara yang memiliki potensi kekayaan alam khususnya pada keragaman hayati, dimana di Indonesia, tanaman dan tumbuh-tumbuhan juga digunakan sebagai obat-obatan oleh masyarakatnya. Sambiloto telah diketahui dan banyak digunakan oleh masyarakat indonesia, sebagai salah satu tanaman herbal yang memiliki khasiat sebagi obat bagi penderita diabetes. Indonesia termasuk satu dari sepuluh negara yang memiliki pasien Diabetes Mellitus (DM) paling banyak di dunia. Studi mengenai pengetahuan dan pengguan sambiloto oleh masyarakat tidak hanya bertujuan untuk berkontribusi pada pemahaman terhadap pengboatan herbal, tetapi juga dapat mendorong dalam penelusuran dan pengembangan sambiloto sebagai obat antidiabetes yang bernilai jual. Studi ini diharapkan dapat memberikan informasi secara ilmiah terhadap tanaman sambiloto yang digunakan oleh masyarakat sebagai antidiabetes. Metode yang digunakan adalah studi literatur dengan mengambil data data dari artikel, database, textbook yang telah dipublikasikan dan diambil dari PubMed, NCBI, elsevier dan lain-lain. Dari hasil yang didapatkan, sambiloto memiki potensi yang besar sebagai antidiabetes
APLIKASI FLOW CYTOMETRY DALAM BIDANG IMUNOLOGI: REVIEW Munir Alinu Mulki; Tiana Milanda; Melisa Intan Barliana
Jurnal Kesehatan Vol. 8 No. 2 (2020): Oktober : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.547 KB) | DOI: 10.55912/jks.v8i2.14

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Flow cytometry merupakan suatu instrumen canggih yang dapat digunakan untuk analisis cepat multi-parameter berbagai karakteristik fisik sel tunggal dalam suatu larutan. Sistem kerja flow cytometry bergantung pada karakteristik fluoresen atau hamburan cahaya dari suatu sel, kemudian dikonversi menjadi sinyal elektronik, dan dianalisis menggunakan komputer. Pendekatan tersebut menjadikan flow cytometry sebagai suatu alat yang baik untuk analisis secara detail suatu populasi sel yang kompleks dalam waktu singkat. Review artikel ini menjelaskan tentang prinsip dasar termasuk komponen dan cara kerja dari flow cytometry, juga menjelaskan tentang analisis data dan aplikasinya dalam bidang imunologi, yaitu immunophenotyping, analisis respon spesifik antigen, analisis sitokin intraseluler, analisis proliferasi, dan analisis apoptosis.
The Effect of Anti-Tuberculosis Drugs on the Effectiveness of Anti-Retroviral Therapy in HIV-AIDS Patients Hasriana Hasriana; Sofa D. Alfian; Tiana Milanda; Rizky Abdulah
Pharmacology and Clinical Pharmacy Research Vol 2, No 1
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.201 KB) | DOI: 10.15416/pcpr.v2i1.16214

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Tuberculosis is the most common opportunistic infection and a leading cause of death among patients who infected by Humman Immunodeficiency Virus (HIV) in developing countries. The purpose of this study was to determine the effect of anti-tuberculosis drugs (ATB) on the effectiveness of anti-retroviral (ARV) therapy in HIV–AIDS infected patients.This study was a prospective cohort study conducted at outpatient clinic of Abepura Hospital during April-June 2015. The data were taken from medical records, which include CD4 level, gen-der, age, education and stage of disease. Statistical analysis was performed using bivariateand multiple regression analysis. A total of 28 subjects was included in this study (7 HIV patients and 21 TB-HIV patients). The results of bivariate analysis showed that there was no significant difference on general characteristics of patients using ARV-ATB and without ATB. Gender, age, occupation, education and the stage of disease did not affect the value of CD4 patients (p>0.005). The result from paired t-test showed that there was no significant difference in the level of CD4 cell, before and after treatment using ARV (p˃0.912), while therapy with ARV-ATB showed significant differences (p˂0.002). In conclusion, this study showed that the combination of ATB and ARV could increase CD4 level up to 72%. Thus, the use of ATB drugs on ARV patients who infected by TB is recommended to reduce disease burden.Keywords: anti-retroviral therapy , HIV- AIDS, tuberculosis
Assessment of Risk Factors of Hepatotoxicity among Tuberculosis Patients Muhammad F. Wardhana; Tiana Milanda; Sri A. Sumiwi
Pharmacology and Clinical Pharmacy Research Vol 3, No 1
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.58 KB) | DOI: 10.15416/pcpr.v3i1.16450

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Tuberculosis is a devastating disease caused by Mycobacterium tuberculosis. If left untreated or not properly treated, it could lead to progressive tissue damage and even death. Short-term chemotherapy containing isoniazid, rifampicin. and pyrazinamide were proven to be very effective in the treatment of TB. However, the concern regarding its potential hepatotoxicity might hinder the completion of treatment. Information regarding risk factor of hepatotoxicity among Indonesian tuberculosis patients were limited. This study aimed to investigate risk factors of hepatotoxicity among Indonesian tuberculosis patients. This was a case-control study with retrospective approach conducted at one of the public hospital in Lampung, Indonesia. We included 320 tuberculosis patients who were classified as case (64 patients who were diagnosed with hepatotoxicity during hospitalization) and control (256 patients). Results of multivariate logistic regression analysis showed that age was significant risk factor of hepatotoxicity (adjusted odds ratio (OR) 1.056, 95% CI 1.0121, 1.091), while longer duration of hospitalization had a 1.4 lower odds of hepatotoxicity compared to control (adjusted OR 0.757, 95% CI 0.682, 0.839) (p<0.005). The results indicated that older patients were more likely to have hepatotoxicity, while patients with shorter duration of hospitalization tend to have higher risk of hepatotoxicity. In conclusion, age was the risk factor associated with hepatotoxicity among tuberculosis patients.Keywords: tuberculosis, hepatotoxicity, age, hospitalization
Kajian Toksisitas Tanaman Genus Garcinia Wa Ode Ida Fitriah; Tiana Milanda; Muchtaridi Muchtaridi
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 17 No. 02 Desember 2020
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v17i2.7691

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Sekitar 80%, alternatif dalam terapi pemeliharaan kesehatan bergantung pada penggunaan obat tradisional. Obat tradisional berasal dari bahan dan teknik pengobatannya yang bersifat alami, sehingga efek sampingnya relatif rendah, memiliki kandungan senyawa yang beraneka ragam bersifat sinergis serta biaya pengobatannya lebih ekonomis. Tanaman genus Garcinia merupakan famili dari Clusiaceae memiliki 400 spesies, 77 spesies diantaranya tersebar di wilayah Indonesia dan tumbuh di daerah tropis. Tanaman ini memiliki potensi besar sebagai obat tradisional dalam  mengobati berbagai jenis kelainan patofisiologi. Pemanfaatan tanaman sebagai obat tradisional sebagian besar didasarkan pada pengalaman, namun informasi tentang bukti keamanan atau toksisitas tanaman obat kepada masyarakat masih terbatas sehingga perlu dilakukan pengkajian toksisitas genus Garcinia. Spesies tanaman dari genus Garcinia yang telah diuji toksisitasnya antara lain, Garcinia brasiliensis Mart., Garcinia xanthochymus Hook.f. ex T.Anderson, Garcinia cowa  Roxb., Garcinia pedunculata Roxb. ex Buch.-Ham., Garcinia husor, Garcinia kola Heckel, Garcinia benthami Pierra, Garcinia mangostana L., Garcinia indica (Thouars) Choisy, Garcinia hombroniana Pierre, Garcinia rubroechinata Kosterm., Garcinia achachairu Rusby, Garcinia atroviridis Griff. ex T. Anders, Garcinia forbesii King, dan Garcinia multiflora Champ. ex Benth.
Co-Authors Adha, Syah Akbarul Adila Srebreneca AFINA DWI RACHMAWATI Ahmad Muhtadi Alam Jenuin Dwipratama Alifia Hasna Hamidah Amaliah Ihsani Anis Yohana Chaerunisaa Anis Yohana Chaerunisaa, Anis Yohana ARIF SATRIA WIRA KUSUMA ASTININGSIH DIAH PRAVITASARI Belinda, Andhara M. Bonar C. Saragih Dewi, Lisa K. Dolih Gozali Dwi Indra Purnomo Ellin Febrina Ellin Febrina Erlianti, Karina FATHIA PEBRIANI Firda Aryanti Widyana Gofarana Wilar Hanny Hafiar Haryanto Dhanutirto Hasriana Hasriana Hasriana Hasriana, Hasriana Hasrianna Hasrianna Hasrianna, Hasrianna Ida P. Santoso Indah Suasani Wahyuni Irma Erika Herawati Irma Erika Herawati Irma Melyani Puspitasari Irma Meylani Puspitasari Ivan S. Pradipta Jutti Levita Keri Lestari Kurniawan Kurniawan Kusuma, Sri A. F. Lisa K. Dewi Maisyarah, Intan Timur Mardiana, Lia Marlia Singgih Wibowo MAYANG KUSUMA DEWI, MAYANG KUSUMA Melisa Intan Barliana Melisa Intan Barliana, Melisa Intan Moelyono Muktiwardojo Mohamad Taufik Ismullah Muchtaridi Muchtaridi Muhaimin Muhaimin Muhammad F. Wardhana Munir Alinu Mulki Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Nasrul Wathoni Nimas T. I. Tarina NURUL ANNISA Nurul Annisa Pradipta, Ivan S. QONITA ZAHRA FADHILA Raden Maya Febriyanti ragavendra ravee Rani Cyinthia Hani Renny Amelia Rhisanandra, Fathia Richie Agusta Iwan Chandra Riezki Amalia Rizky Abdulah Ronny Lesmana Saragih, Bonar C. Septiyani Mustikawati Sjafril, Astri K. Soemarie, Yulistia Budianti Sofa D. Alfian Sofa D. Alfian, Sofa D. Sri A. F. Kusuma Sri A. F. Kusuma Sri A. Sumiwi Sri Adi Sumiwi Sri Agung Fitri Kusuma, Sri Agung Sriwidodo Sriwidodo Steffi Liem Suharyani, Ine Sukmawati Sukmawati Sukmawati Sukmawati Sulistiyaningsih Sulistiyaningsih Sumiwi, Sri A. Supriyatna Supriyatna Susilawati, Yaswinar Syah Akbarul Adha SYIFA HANIFAH TERESYA PUTERI Tina Rostinawati TUTUS GUSDINAR Uce Lestari Vesara A. Gatera Wa Ode Ida Fitriah Wardhana, Muhammad F. Wijaya, Viriyanata Yasmiwar Susilawati Yaswinar Susilawati Yuli Andriani Yuni Elsa Hadisaputri Yuyun Wahyuni