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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Naturalis : Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan Jurnal Ilmu-Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat ELINVO (Electronics, Informatics, and Vocational Education) Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan JMM (Jurnal Masyarakat Mandiri) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Jurnal AGRISEP: Kajian Masalah Sosial Ekonomi Pertanian dan Agribisnis

Atra Romeida

Universitas Bengkulu

Author-ID : 3266111

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Social Sciences Other

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IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.Â On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.Â Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.Â Â The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.Â The initial step of the research was to select random primers could positively amplify the maize DNA.Â The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.Â Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.Â Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.102

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago. On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation. Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants. The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents. The initial step of the research was to select random primers could positively amplify the maize DNA. The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA. Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents. Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.

Rooting of Mini-Cuttings of guava cultivars (Psidium guajava L.) Treated with IBA under Misting Irrigation Supanjani, Supanjani; Rahayu, Hatari; Suprijono, Eko; Romeida, Atra; Yulian, Yulian
Akta Agrosia Vol 23, No 1 (2020)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.23.1.27-32

Â Â Â Â Â Â Guava (Psidium guajava L.) is a plant whose fruit is very popular in Indonesia because it contains high vitamin C and its leaves can be used as herbal medicine. Orchard expansion of selected cultivars and fruit production of guava can be achieved vegetatively through Â propagation by using mini-cuttings with the use of Indole Butyric Acids (IBA). A study was conducted to compare rooting success of mini-cuttings of herbaceous stem of four guava cultivars with IBA treatment. Four guava cultivars tested were Bengkulu Round, Getas, Crystal, and Bangkok.Â Mini-cuttings were immersed in IBA solution at 0 ppm or 1000 ppm. Cultivar and IBA treatments were arranged factorially in a randomized complete Â block design with Â 3 replications, each consisting of 25 cuttings. Variables observed included percentage of original-leaf shedding, percentage of emerging shoots, percentage of sprouting cuttings, new leaf number, root number, root length, and percent of rooted cuttings. The results demonstrated that cultivars affected rooting percentage, sprouted percentage, percentage of original-leaf fall, and number of root.Â Getas had highest rooting and sprouting percentages, and showed lowest number ofÂ original-leaf shedding; whereas, Bangkok demostrated greatest number of root.Â IBA treatment increased the number of root and the number of original-leaf shedding, but reducing rooting percentage of mini-cutting. There was no interaction between cultivar and IBA teratment.

Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid

Optimasi Pertumbuhan dan Multiplikasi Lini Klon PLBs Anggrek Spathoglottis plicata Blume melalui Modifikasi Komposisi Medium MS dan Sitokinin Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Jurnal Hortikultura Indonesia Vol. 4 No. 1 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTIn order to enhance the multiplication of Protocorm Like Bodies (PLBs) of Spathoglottis plicataorchid in vitro, several combinations of vitamin, sucrose concentration, and cytokinin were tested. Thisexperiment was arranged in Completely Randomized Design (CRD) with two factors. The first factor was combination of vitamin composition and sugar concentrations in the medium which consisted of four different combinations (J1 = vitamin MS + sucrose 30 g L-1, J2 = vitamin B5 + sucrose 30 g L-1, J3= vitamin MS + sucrose 40 g L-1, J4 = vitamin B5 + sucrose 40 g L-1). The second factor was seven combinations of cytokinin types and its concentrations (S0 = without cytokinin (control), S1 = 20 μM BA, S2 = 40 μM BA, S3 = 20 μM kinetin, S4 = 40 μM kinetin, S5 = 75 ml L-1 coconut milk, and S6 = 150 ml L-1 coconut milk). PLBs were used as explant and were grown on MS solid medium containing various vitamin, sucrose, and cytokinin combinations as mentioned above. Growth and multiplication of PLBs were based on the number of PLBs per explant, number of plantle ts per explant, number of roots formed, plantlet height and visual appearance and performa nce of the observations at 6 MST. The best growth and multiplication of PLBs orchid S. plicata was produced on MS medium modified with B5 vitamins and 30 g L-1 sucrose, followed by those and on MS medium enriched with 75 ml L-1 coconut milk and on MS medium supported by 20 μM BA.Key words : B5, benzyl adenine, coconut water, in vitro , orchid, sugar concentration, vitamin compositionABSTRAKPercobaan ini bertujuan untuk mendapatkan formulasi kom posisi vitamin dan konsentrasi gula medium,serta jenis dan konsentrasi sitokinin terbaik dalam menginduksi pertumbuhan dan multiplikasi lini klon PLBsanggrek Spathoglottis plicata dalam jumlah yang besar secara in vitro. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Eksplan yang digunakan adalah PLBs yang ditanam pada medium Murashige dan Skoog (MS) yang telah dilakukan modifikasi sesuai dengan perlakuan. Faktor pertama adalah formulasi komposisi vitamin dan konsentrasi gula yang terdiri dari empat macam formulasi yaitu J1 = vitamin MS + gula 30 g L-1, J2 = vitamin B5 + gula 30 g L-1, J3= vitamin MS + gula 40 g L-1, J4 = vitamin B5 + gula 40 g L-1. Faktor kedua adalah penambahan sitokinin (3 jenis dengan 2 taraf konsentrasi) yang terdiri dari 7 kombinasi perlakuan yaitu S0 = tanpa sitokinin (kontrol), S1 = BA 20 μM, S2 = BA 40 μM, S3 = kinetin 20 μM, S4 = kinetin 40 μM, S5 = air kelapa 75 ml L- 1, dan S6 = air kelapa 150 ml L-1. Pertumbuhan dan multiplikasi PLBs anggrek S. plicataterbaik dihasilkan pada medium MS dengan modifikasi vitamin B5 dan konsentrasi gula 30 g L-1dan pada medium MS dengan penambahan air kelapa 75 ml L- 1 serta pada medium MS dengan penambahan BA 20 μMdengan kriteria jumlah PLBs akhir dan jumlah planlet akhir tertinggi, jumlah akar dan tinggi tanaman serta penampilan visual hasil pengamatan pada 6 minggu setelah tanam.Kata kunci : air kelapa, anggrek, B5, benzyl adenin, in vitro, komposisi vitamin, konsentrasi gula

Pemberdayaan Masyarakat Pesantren Ar-Rahmah, Rejang Lebong dalam Memanfaatkan Lahan Pekarangan dengan Budi Daya Bawang Merah Marlin Marlin; Antoni Sitorus; Muhamad Solihin; Atra Romeida; Reny Herawati
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 6 No. 1 (2020): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.6.1.53-61

The community empowerment in the area of Ar-Rahmah pesantren, Air Meles Atas Village, Rejang Lebong Regency, Bengkulu was intended to increase the income of pesantren to finance poor students, and at the same time to enhance the ability of pesantren in carrying out educational and teaching activities. Community service activities carried out in the form of assistance activities in shallot cultivation in the yard of the Ar-Rahmah Pesantren. The assistance program is carried out for 4 months, with such stage of activities as: the preparation stage for managers, teachers, students and farmers around the pesantren, seed selection, land preparation, planting, maintenance, harvesting, and post-harvest. The evaluation result showed that the community participation and involvement in the shallot cultivation assisting program were considered high. The highest participation rates were during the selection of seed activity (73%) and planting activity (100%). Meanwhile, the lowest participation rate was during maintenance activities (27%). The evaluation results showed the level of participants’ satisfaction is high (90‒100%) on the implementation of assistance activities. The harvested shallots could increase the income of the boarding school which can be used to help the education cost and education progress of the boarding school. The participation of the entire community would be encouraged so that the success of empowerment program could be felt by the entire community. The community empowerment program need to be carried out continuously, so it would enhance the welfare of and self-sufficiency of the community.

Adopsi Petani Terhadap Teknologi Jajar Legowo Padi Sawah Di Kelurahan Rimbo Kedui Kecamatan Seluma Selatan Kabupaten Seluma Yeni Herlina; Mohammad Chozin; Atra Romeida
Naturalis: Jurnal Penelitian Pengelolaan Sumber Daya Alam dan Lingkungan Vol. 8 No. 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.8.2.9217

Program GP-PTT sebagai kelanjutan dari Program SL-PTT yang menerapkan teknologi jajar legowo merupakan suatu upaya yang telah dilakukan oleh pemerintah untuk meningkatkan produktifitas lahan persawahan yang ada melalui pemberdayaan petani. Untuk mengetahui tingkat keberhasilan teknologi jajar legowo dalam program tersebut maka perlu dievaluasi tingkat penerapan komponen-komponennya yang dipengaruhi oleh berbagai faktor. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan mengevaluasi faktor–faktor sosial ekonomi (umur, pendidikan, luas lahan, status kepemilikan lahan, jumlah anggota keluarga, pengalaman usahatani, pendapatan) dan persepsi petani terhadap teknologi jajar legowo padi sawah di Kelurahan Rimbo Kedui kecamatan Seluma Selatan Kabupaten Seluma. Penentuan daerah penelitian dilakukan secara sengaja yaitu petani padi sawah yang telah mengikuti kegiatan program SL-PTT dan GP-PTT yang menerapkan teknologi jajar legowo. Data yang digunakan adalah data primer dan data sekunder yang diperoleh dari hasil survei di lapangan. Metode penentuan sampel menggunakan teori yang dikemukakan oleh Arikunto Suharsimi untuk jumlah populasi diatas 100 maka diperoleh sampel sebanyak 40 orang petani. Penentuan faktor-faktor yang memetakan tingkat adopsi dilakukan dengan analisis regresi logistik dengan transformasi logit. Hasil analisis secara deskriptif menunjukkan bahwa sebanyak 98,82% petani mempunyai persepsi baik terhadap sifat inovasi teknologi jajar legowo dan 62,5% petani menerapkan sesuai anjuran sedangkan 37,5% petani menerapkan tidak sesuai anjuran. Hasil analisis logistik menunjukkan bahwa pendidikan dan pendapatan usahatani merupakan faktor penting dalam menentukan tingkat adopsi teknologi jajar legowo oleh petani.Kata Kunci : adopsi, petani, jajar legowo, padi sawah

PERTUMBUHAN DAN HASIL TANAMAN MELON (Cucumis melo L.) PADA KOMPOSISI MEDIA TANAM DAN FREKUENSI PEMUPUKAN YANG BERBEDA Muhammad Iqbal; Faiz Barchia; Atra Romeida
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 21, No 2 (2019)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.21.2.108-114

[GROWTH AND YIELD OF MELON PLANT (Cucumis melo L.) IN DIFFERENT COMPOSITION OF PLANT MEDIA AND DIFFERENT FREQUENCY OF NPK APPLICATION]. This study aims to determine the best combination of planting media composition and frequency of NPK fertilization on the growth and yield of melon plants. The research was conducted in October 2018 - January 2019 on the land located in Bentiring, Muara Bangkahulu District, Bengkulu City. The design used in this study was a Completely Randomized Design (CRD) consisting of two factors and three replications. The first factor was the composition of the media for plant growth, which consists of three different media composition, namely: sand: soil: manure (30%: 50%: 20%), sand: soil: manure (10%: 60%: 30%), and sand: soil: manure (20%: 70%: 10%). The second factor is the frequency of NPK fertilization consisting of four types of fertilization, namely: 1 time NPK fertilization during planting, 2 times NPK fertilization during planting and 10 days after planting (dap), 3 times NPK fertilization during planting, 10 dap and 20 dap, and 4 times NPK fertilizing during planting, 10 dap, 20 dap and 30 dap. The dose given is 15 g/plant (equivalent to 800 kg/ha) and NPK fertilizer given in the form of compound fertilizer N: P: K 16:16:16. The results showed that the best composition of the planting media was found in the treatment of sand media: soil: manure (10%: 60%: 30%). The best fertilizing frequency was found in the treatment of NPK fertilizing 4 times during planting, 10 dap, 10 dap, 30 dap, and the combination of both produces the most substantial plant length, weight, and fruit circumference

Soybean Performance on Types of Compose and Dosage of Urea Fertilizer in Coastal Lands of Bengkulu Hesti Pujiwati; Widodo Widodo; Atra Romeida; Dotti Suryati; Muhimmatul Husna; Wahyu Hidayat; Edi Susilo
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 19 No 1 (2021): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v19i1.1794

One of the efforts to increase soybean production in Bengkulu is through the use of coastal land. Coastal land improvement is carried out by adding compost fertilizers. In order to meet the nutrient needs for soybean plants in coastal lands, a combination of compost and urea is needed. This study aims to determine the performance of soybean in the application of various types of compost and doses of urea and determine the type of compost and the best dose of urea in coastal lands. The study was conducted in July-August 2020. The study used a 2-factor and 3-replications in Completely Randomized Block Design, the first factor was the type of compost: P1 = Widelia trilobata; P2 = Tithonia diversivolia; P3 = oil palm empty bunches, P4 = water hyacinth. The second factor is the dose of Urea: N0 = 0 kg.ha-1, N1 = 25 kg.ha-1, N2 = 50 kg.ha-1, N3 = 75 kg.ha-1. The soybean used was the variety of Direng 1. The results showed that the type of compost and the dose of urea were not significantly different in the soybean plant performance variables. The application of 4 types of compost as much as 10 tons ha-1 in coastal lands showed poor growth and yield a little. Thitonia compost gives the highest yields of 0.5 ton.ha-1 and 0.48 ton.ha-1 when giving urea 75 kg.ha-1. Soybean yields are still very low, so it is necessary to double the dose of urea in coastal lands. Keywords: Coastal Land, Direng 1, Compost and Urea.

EVALUASI DAN OPTIMALISASI TEKNIK OPERASIONAL SAMPAH KECAMATAN AMEN KABUPATEN LEBONG Purwo Setyadi; Satria Putra Utama; Damres Uker; Agus Martono; Atra Romeida
Naturalis: Jurnal Penelitian Pengelolaan Sumber Daya Alam dan Lingkungan Vol. 11 No. 1 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.11.1.21163

Penelitian ini bertujuan untuk (1) mengetahui kondisi eksisting teknik operasional pengelolaan sampah Kecamatan Amen, meliputi tingkat pelayanan, daerah pelayanan, teknik operasional dimulai dari pewadahan, pengumpulan, pemindahan, pengangkutan hingga Pemrosesan akhir, dan jumlah serta jenis armada yang digunakan, dan (2) mengevaluasi kondisi eksisting dan merencanakan teknik operasional pengelolaan sampah Kecamatan Amen pengembangan untuk 10 tahun ke depan. Penelitian dilakukan di wilayah Kecamatan Amen. Identifikasi dan evaluasi kondisi eksisting teknik operasional pengelolaan sampah dilakukan dengan menghitung data timbulan dan komposisi atau karakteristik sampah Kecamatan Amen. Sedangkan perencanaan teknik operasional pengelolaan sampah pengembangan untuk 10 tahun ke depan yang dilakukan meliputi perencanaan peningkatan tingkat pelayanan dan sistem teknik operasional pengelolaan sampah dimulai dari sumber/pewadahan, pengangkutan, tempat sementara, pemindahan ke tempat pemrosesan akhir. Hasil penelitian menunjukkan bahwa teknik sampah di Kecamatan Amen belum sepenuhnya sesuai dengan SNI 19-2454-2002, dengan tingkat pelayanan sebesar 20%, dan jumlah pewadahan 150 buah, alat pengangkutan berupa motor roda tiga sebanyak satu buah, dan hanya memiliki satu buah TPS di Desa Sungai Gerong, selain itu terdapat 1 buah alat pemindah berupa dump truck dengan kapasitas 6 m3 yang dibawa ke TPA Air Kopras Kecamatan Pinang Belapis Kabupaten Lebong. Timbulan sampah rata-rata yang dihasilkan masyarakat Kecamatan Amen adalah 2,89 liter/orang/hari dengan tooal sampah 24,38 m3/hari. Direncakanakan dalam pengembangan teknik operasional sampah di tahun 2020 sudah melayani 100 %, dan pada akhir tahun perencanaan (Tahun 2030) dibutuhkan jumlah pewadahan sebanyak 777 buah, jumlah motor roda tiga sebanyak 21 buah, sedangkan untuk jumlah TPS sebanyak 6 buah, dan untuk dump truck hanya menambahkan 2 buah dari kondisi eksisting. Kata kunci: Kata Kunci : Evaluasi, Identifikasi, Teknik Operasional Sampah

Co-Authors Afrisa, Hendrio Agus Martono Agus Martono Agus Purwito Agus Susanto AGUS SUSATYA Almunawar, Said Agil Alnopri Antoni Sitorus Anwar, Guswarni Arie Vatresia Bambang Gonggo Murcitro Bambang Purnomo Barchia, M. Faiz Damres Uker Defi Ermayendri Dewi Sukma Dotti Suryati Dotty Suryati Dwi W Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Edi Susilo Eko Suprijono Eko Suprijono Faiz Barchia Febriyani, Sustri Gunggung Senoaji Helfi Eka Saputra Herison, Catur HESTI PUJIwATI Hidayat Hidayat Indarwanto Indarwanto Indarwanto Marlin Marlin Marwanto Marwanto, Marwanto Meko Gustian Merakati Handajaningsih Mimi Sutrawati Mohammad Chozin Mohammad Chozin, Mohammad Muhamad Solihin Muhammad Iqbal Muhimmatul Husna Prasetyo Prasetyo Purwo Setyadi Raden Mohamad Herdian Bhakti Rahayu, Hatari Rahayu, Hatari Rani, Eka Reflis Reny Herawati Rustikawati Rustikawati Rustikawati Saiful Akhyar Lubis Saputra, Eko Adi Satria Putra Utama Seprina Dwi Cahyani Sukisno Supanjani, Supanjani Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono Hadi Sutjahjo Teguh Adiprasetyo Usman Siswanto Wahyu Hidayat Wahyu Hidayat Wati, Shinta Puspita Widodo Widodo Wuri Prameswari Yeni Herlina Yudhi Harini Bertham, Yudhi Harini Yulian, Yulian Yulian, Yulian Yuristia, Rahmi Yuwana Yuwana Yuwana

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