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Journal : specta journal of technology

Antioxidant Activity Test Of Red Spinach Leaf (Amaranthus Tricolor L.) Extract And Fraction With Methode (2,2-Diphenyl-1 Picrylhydrazyl) Noviyanti; Asterina, Yolanda; Khairunnisa, Anita
SPECTA Journal of Technology Vol. 9 No. 2 (2025): Specta Journal of Technology
Publisher : LPPM ITK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35718/specta.v9i2.8481340

Abstract

Abstract Red spinach (Amaranthus tricolor L.) is a leafy vegetable known for its high antioxidant potential due to its bioactive compounds. This study aimed to evaluate the antioxidant activity of extracts and fractions of red spinach leaves using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Extraction was conducted through cold maceration with ethanol 96%, followed by fractionation using solvents of varying polarity: n-hexane, ethyl acetate, and water. Phytochemical screening revealed the presence of alkaloids, flavonoids, quinones, saponins, steroids, and tannins, which contribute to the plant's antioxidant properties. The antioxidant activity of the ethanol extract and fractions was assessed based on their IC50 values. The ethanol extract demonstrated moderate antioxidant activity with an IC50 of 122.09 µg/mL, while the n-hexane and water fractions showed similar moderate activity with IC50 values of 117.33 µg/mL and 124.36 µg/mL, respectively. The ethyl acetate fraction exhibited strong antioxidant activity with an IC50 value of 64.77 µg/mL, attributed to the presence of semi-polar compounds such as flavonols. The results confirmed the significant free radical scavenging potential of red spinach, particularly in its ethyl acetate fraction. This study highlights the potential of red spinach leaves as a natural source of antioxidants with applications in the pharmaceutical, health, and food industries. Keywords: Red spinach, Amaranthus tricolor L., DPPH, antioxidant activity, IC50.
Co-Authors Agus Prasetyawan Ainy, Afra Nur Alfira S Amalia , Rosemila Amalia, Rosemila AMBAR WIDYA LESTARI Andriani Antonio Constantino Soares Ari Lutfia Sari Ari Widyaningsih Arianti, Gisela Arnando Asma, Nurul ASNI Asterina, Yolanda Aulia Rosida, Nabila Azizah, Maulia Salsanabila Azwar, Yessi Badrudin Kurniawan Basuki Batubara, Hamidah Sari Bella, Ivvon Septina Berliani, Dedek Bunga Tiara Carolin DIAN ARLUPI UTAMI Dirgayusa Febrianaga, Pipit Eliyah Emy Susanti EVA AULIA MUKTI Eva Tresanita Damanik Faisal R Dongoran, Faisal R Fitrianingrum, Aditya Pratiwi Florida Gading Gamaputra Handayani, Sri Hidayatullah, Agus Hikmah, S.E., M.Si. Hutasoit, Eva Santi Ice Irawati Imanudin Siregar Indera Hayati, Aulia Dian Isnaini Fitri Effendi Jayanti Juliani, Wahyuni Karimah, Arzetya Fitri Al Keristanti, Ruri Khaatamal Awlad Khairunnisa, Anita Khummad Robby, Tasbikhy Kusuma Dewi, Novian Leni Utami Lilis Suwandari Lina Haryani Lukmawati, Luthfi Rizky Mar'atus Sholikhah Matulessy, Stella Maria Mauizatul Hasanah Mega Dewi Lestari Melgita Sari Nurjayanti Saputri Melisa Misriningsih, Dwi Cahyani Mohammad Yogi Hamdi Mur'ah Murni Nanik Cahyati Natasha, Ayu Naufal Hanif Ramadhan Nur Ichani Nur Rachma, Dita Laela Nurkhairina Nurmayana Pardosi, Renhad Andreas Permana, Handy Januar Pinata, Putri Prasetyo Isbandono Punarbawa, Gede Putri Fatahillah, Ratu Egalitarian Putri Nabilah Ranti Widayanti Ratna Sari, Monika Retno Widuri, Aliffionia Sindy Hendrati Rice Haryati Rinanti, Maghalita Rinanti Rizka Hadya Robby Cokro Buwono Rosyidah, Neneng Aida Salinan Sarasati, Inda Siti Maesaroh Sophia Sri Mustika Sujarwo Putri, Tarisa Sukarsono, Faza Muhammad Syalmah Tahlia Wahyuning Tias Takharus Awal, Annisa Zahra Tanosida K Theresa Heidi A Kanadjara Triastianti, Rita Dewi Wahyuni, Rika Sri Warsiyah Warta, Waska WENI ROSDIANA Wibisono, Haryo Kunto Widyaningsih, Triatmi Sri Widyaningtyas, Dwi Wilujeng, Vianti Winata, Muhammad Danu Yekti Murbarani Yeni Rosyeni Yulistia Yuni Lestari Yuni Lestari Yusuf, Salsabila Zalikha Zulfahmi