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Journal : Specta Journal of Technology

 1 
Antioxidant Activity Test Of Red Spinach Leaf (Amaranthus Tricolor L.) Extract And Fraction With Methode (2,2-Diphenyl-1 Picrylhydrazyl) Noviyanti; Asterina, Yolanda; Khairunnisa, Anita
SPECTA Journal of Technology Vol. 9 No. 2 (2025): Specta Journal of Technology
Publisher : LPPM ITK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35718/specta.v9i2.8481340

Abstract

Abstract Red spinach (Amaranthus tricolor L.) is a leafy vegetable known for its high antioxidant potential due to its bioactive compounds. This study aimed to evaluate the antioxidant activity of extracts and fractions of red spinach leaves using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Extraction was conducted through cold maceration with ethanol 96%, followed by fractionation using solvents of varying polarity: n-hexane, ethyl acetate, and water. Phytochemical screening revealed the presence of alkaloids, flavonoids, quinones, saponins, steroids, and tannins, which contribute to the plant's antioxidant properties. The antioxidant activity of the ethanol extract and fractions was assessed based on their IC50 values. The ethanol extract demonstrated moderate antioxidant activity with an IC50 of 122.09 µg/mL, while the n-hexane and water fractions showed similar moderate activity with IC50 values of 117.33 µg/mL and 124.36 µg/mL, respectively. The ethyl acetate fraction exhibited strong antioxidant activity with an IC50 value of 64.77 µg/mL, attributed to the presence of semi-polar compounds such as flavonols. The results confirmed the significant free radical scavenging potential of red spinach, particularly in its ethyl acetate fraction. This study highlights the potential of red spinach leaves as a natural source of antioxidants with applications in the pharmaceutical, health, and food industries. Keywords: Red spinach, Amaranthus tricolor L., DPPH, antioxidant activity, IC50.
Co-Authors Agus Prasetyawan Ainy, Afra Nur Amalia , Rosemila Amalia, Rosemila AMBAR WIDYA LESTARI Andriani Angga Domeos Manggara Antonio Constantino Soares Ari Widyaningsih Arianti, Gisela Arnando Asma, Nurul ASNI Asterina, Yolanda Aulia Rosida, Nabila Azizah, Maulia Salsanabila Azwar, Yessi Badrudin Kurniawan Basuki Batubara, Hamidah Sari Bella, Ivvon Septina Berliani, Dedek Bunga Tiara Carolin DIAN ARLUPI UTAMI Dirgayusa Febrianaga, Pipit Eliyah Emy Susanti EVA AULIA MUKTI Eva Tresanita Damanik Faisal R Dongoran, Faisal R Fitrianingrum, Aditya Pratiwi Florida Gading Gamaputra Handayani, Sri Hidayatullah, Agus Hikmah, S.E., M.Si. Hutasoit, Eva Santi Ice Irawati Imanudin Siregar Indah Puspitasari Indera Hayati, Aulia Dian Isnaini Fitri Effendi Jayanti Juliani, Wahyuni Karimah, Arzetya Fitri Al Keristanti, Ruri Khairunnisa, Anita Khummad Robby, Tasbikhy Kusuma Dewi, Novian Leni Utami Lina Haryani Lukmawati, Luthfi Rizky Mar'atus Sholikhah Maratun Shoaliha Matulessy, Stella Maria Mauizatul Hasanah Maulida Mega Dewi Lestari Meissi Kusuma Wardhani Melgita Sari Nurjayanti Saputri Melisa Misriningsih, Dwi Cahyani Muhammad Zaini Mur'ah Murni Murniati Nanik Cahyati Natasha, Ayu Naufal Hanif Ramadhan Norwanda Nur Rachma, Dita Laela Nurkhairina Nurlina Nurmayana Nurwahidah, Andi Tenri Oskar Pardosi, Renhad Andreas Permana, Handy Januar Pinata, Putri Prasetyo Isbandono Punarbawa, Gede Purnamasari, Ade Irma Putri Fatahillah, Ratu Egalitarian Ranti Widayanti Ratna Sari, Monika Retno Widuri, Aliffionia Sindy Hendrati Rice Haryati Rinanti, Maghalita Rinanti Rita Dewi Triastianti Rizka Hadya Robby Cokro Buwono Rosyidah, Neneng Aida Salinan Sarasati, Inda Siti Maesaroh Sophia Sri Mustika Sujarwo Putri, Tarisa Sukarsono, Faza Muhammad Suwiase, Putu Syalmah Tahlia Wahyuning Tias Takharus Awal, Annisa Zahra Tanosida K Tohidi, Edi Triatmi Sri Widyaningsih Wahyuni, Rika Sri Warsiyah Warta, Waska Wati, Norlidia WENI ROSDIANA Wibisono, Haryo Kunto Widyaningtyas, Dwi Wilujeng, Vianti Winata, Muhammad Danu Yekti Murbarani Yeni Rosyeni Yulistia Yuni Lestari Yuni Lestari Yusuf, Salsabila Zalikha Zulfahmi