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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Biologi Undiksha Agrikultura BIOTROPIA - The Southeast Asian Journal of Tropical Biology Alchemy : Journal of Chemistry Jurnal Harpodon Borneo Biogenesis: Jurnal Ilmiah Biologi Biotropika Jurnal Pendidikan Biologi Indonesia Jurnal IPTEK QUANTUM: Jurnal Inovasi Pendidikan Sains Jurnal Pengabdian Pada Masyarakat Bioedukasi: Jurnal Pendidikan Biologi Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Jurnal Pengabdian Masyarakat Borneo Jurnal Borneo Saintek Jurnal Riset Biologi dan Aplikasinya Soeropati: Journal of Community Service Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati Borneo Journal of Biology Education (BJBE) Biopedagogia Al Kawnu : Science and Local Wisdom Journal Biosaintifika: Journal of Biology & Biology Education Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Endik Deni Nugroho
Jurusan Biologi, Universitas Borneo Tarakan
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The assistance of Pesanggem in the Prevention of Coffee Pests through Vegetable Pesticides Coffee from Amethyst Mountain (Brugmansia Suaveolens Bercht. & J. Presl) at Taman Kehati Sapen Nusantara Mount Arjuno Pasuruan: Pendampingan Pesanggem dalam Pencegahan Hama Kopi melalui Pestisida Nabati Kopi dari Kecubung Gunung (Brugmansia Suaveolens Bercht. & J. Presl) di Taman Kehati Sapen Nusantara Gunung Arjuno Pasuruan Wibisono, Mulyono; Fafit Rahmat Aji; Amang Fathurrohman; Endik Deni Nugroho
Jurnal Soeropati Vol 4 No 2: Mei 2022
Publisher : LPPM Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/js.v4i2.3422

Abstract

Pesanggem Taman Kehati Sapen Nusantara (TKSN) is a farming community in the Sapen Forest Area of ​​Mount Arjuno, Pasuruan. Coffee agroforestry is the main commodity for forest farmers in the TKSN area. For pest prevention, they have been using chemical pesticides to prevent the Coffee Fruit Borer. Through the Community Based Research approach, the companion team together with the community have succeeded in innovating vegetable pesticides coffee by utilizing Brugmansia Suaveolens Bercht. & J. Presl and Swietenia macrophylla King. as the main ingredients that are easily found in Taman Kehati Sapen Nusantara into environmentally friendly coffee pesticide products.
Diversity of bird agroforestry species in Sapen Nusantara Conservation Park of Mount Arjuno, Pasuruan Nugroho, Endik Deni; Rahayu, Dwi Anggorowati; Ainiyah, Roisatul; Fathurrohman, Amang; Ahwan, Zainul; Dayat, Muhammad; Wibisono, Mulyono; Aji, Fafit Rahmat; Kasiman, Kasiman
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 8 No. 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.1059

Abstract

The Sapen Nusantara Conservation Park is agroforestry that has certain vegetation. The vegetation in Sapen Nusantara Protected forest park seems to support the existence of birds as wildlife that need a living habitat. The presence of birds in the Sapen Nusantara Protected Forest Park is very important in supporting agroforestry vegetation. However, until now the diversity of bird species there has not been identified and recorded properly. The forest is one of the buffer forests of Mount Arjuno protection forest, the ecosystem must be protected. This study aims to analyze the diversity of bird species in the agroforest forest area in Sapen Nusantara Conservation forest park, Ledug Village, Pasuruan. The research was conducted in July 2021 using the direct observation method (point count) on ecotourism routes by calculating and recording the trails traversed using GPS, sampling by observing walking along the path/road accompanied by predetermined observation points, identification primarily based totally on MacKinnon et al., (2010) and information on the status of its protection. Data analysis used the Shannon-Wiener diversity index, evenness index, and species richness index. The results of the study identified 18 bird species from 16 families with a total of 169 individuals. Of all the birds found, 18 species are listed in IUCN status, and 4 are listed in the protected status PP No. P.106 of 2018 and 3 species are listed in the CITES category Appendix II. It is known that the Shannon diversity index (H'=2,547) is in the medium diversity category. species evenness index (E=0.9) with a high evenness category and stable community. Then the specific wealth index (R=3.31) with the category of species richness is classified as medium. The presence of birds in the Sapen Nusantara Conservation Park Forest indicates that the ecosystem in the forest is still maintained and this cannot be separated from the role of the community, especially the Ledug Lembaga Masyarakat Desa Hutan (LMDH), which manages and maintains the forest.
Identifikasi Kandungan Volatil Ekstrak Etanol Benalu Alpukat (Dendrophthoe pentandra) Sebagai Kandidat Antibiotik Alami Tuberkulosis Ainiyah, Roisatul; Nugroho, Endik Deni; Ardiansyah, Reza; Hamdani, Khoirul; Tarmudi, Imam; Kuswanto, Wawan; Hadi, Lukman
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13326

Abstract

Tuberculosis is an infectious disease that spreads through the air and is caused by the bacterium Mycobacterium tuberculosis. To combat it, synthetic drugs are commonly used, which can have adverse effects, making natural alternatives like avocado mistletoe (Dendrophthoe pentandra) necessary. This study aims to explore potential tuberculosis compounds from D. pentandra. The method used in this research is an exploratory descriptive method. The identification of compound content in the ethanol extract of D. pentandra leaves was performed using GC-MS, along with potential testing as an antituberculosis agent through in silico molecular docking. The research results showed that there were 13 volatile compounds contained in the ethanol extract of D. Pentandra leaves, predominantly consisting of propane, 1-methoxy, with a concentration of 9.39%. D. pentandra has the potential as a ClpP1P2 antituberculosis agent with four promising compounds:2-amino-1-(5-bromo-2-pyridinyl)-4-(2,4-dichlorophenyl)-7,7-dimethyl-5-oxo-1,4,5,6,7,8-hexahydro-3-quinolinecarbonitrile, stigmasterol acetate, β-sitosterol acetate, and γ-sitosterol. PASSOnline predictions support the potential of ethanol extract of D. Pentandra leaves as anti-tuberculosis and anti-mycobacteria agent.
Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia Panjaitan, Viona Angelina Erlan; Rangga, Adam Dwi; Sulung, Cahya Ajeng Valenta Tresna; Rahayu, Dwi Anggorowati; Khaleyla, Firas; Nugroho, Endik Deni; Rusdianto, Rusdianto; Mamat, Noorhidayah Binti
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.26663

Abstract

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.
FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA Rahayu, Dwi Anggorowati; Kuntjoro, Sunu; Budijastuti, Widowati; Winarsih, Winarsih; Ambarwati, Reni; Nugroho, Endik Deni; Basith, Abdul; Kurniawan, Nia; Haryono, Haryono
BIOTROPIA Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1942

Abstract

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.
Pendekatan Fenetik Taksonomi dalam Identifikasi Kekerabatan dan Pengelompokkan Ikan Genus Tor di Indonesia RAHAYU, DWI ANGGOROWATI; NUGROHO, ENDIK DENI
Bioedukasi: Jurnal Pendidikan Biologi Vol 7, No 1 (2014): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v7i1.2844

Abstract

Ikan Tor merupakan ikan air tawar lokal Indonesia dan termasuk ikan terancam punah. Upaya konservasi yang harus dilakukan salah satunya adalah penentuan kekerabatan berdasarkan pendekatan fenetik. Hal ini dikarenakan kedudukan taksonomi dan klasifikasi diantara genus Tor masih rancu. Sumber data yang digunakan adalah karakter meristrik, morfometrik, morfologi secara umum, dan karakter khusus penentu spesies. Karakter khusus penentu spesies diantara ikan genus Tor adalah keberadaan dan ukuran cuping pada bibir bawah. Sampel ikan Tor diambil dari Telaga Banyu Biru Pasuruan (nama lokal ikan Sengkaring dan Tambra), serta spesies acuan (Tor tambraides dari Kalimantan Barat, Tor duoronensis dari Padang, and Tor soro dari Sumatra Utara). Fenogram dibentuk berdasarkan metode UPGMA. Berdasarkan fenogram yang terbentuk diperoleh 3 kelompok kekerabatan dari yang terdekat hingga terjauh dan 2 kelompok apomorfi serta 4 kelompok automorfi. Percabangan pertama terdiri dari dua subklad yaitu Tor duoronensis dan Sengkaring memiliki kesamaan 100%, dan Tambra merupakan sister clad dari Tor duoronensis yang didukung dengan nilai similaritas 92,9%. Subklad kedua terdiri dari Tor Tambra dan Tor soro (Apomorfi B) dengan nilai similaritas 59%, sedangkan Tor tambraides memiliki kekerabatan terjauh dengan indeks kesamaan sebesar 45, 625%. Ikan Sengkaring dan Tambra menunjukkan pola pengelompokan yang dekat dengan Tor duoronensis berdasarkan tujuh karakter morfometrik pembeda utama. Ketujuh karakter pembeda utama tersebut adalah ukuran cuping, SL (panjang standart), MXBL (panjang sungut rahang atas), CPL (panjang batang ekor), PDL (panjang sebelum sirip dorsal), IW (jarak antar mata) dan SNL (panjang moncong). Hasil analisis morfometrik menunjukkan bahwa ikan Sengkaring dan Tambra mutlak bukan merupakan ikan Tor Tambraides maupun Tor soro dikarenakan ukuran tubuh yang berbeda secara signifikan.
Co-Authors - Ibrahim, - Abdul Basith Achmad Zultan M Adelyn Salurapa Adelyn Salurapa Ahwan, Zainul Aidil Adhani Ainiyah, Roisatul Aji, Fafit Rahmat Aji, Fatit Rahmar Alfrida Alfrida Amang Fathurrohman Andari, Kadek Dewi Wahyuni Andari, Kadek Dewi Wahyuni Azrianingzih, Rodiyati Azrianingzih, Rodliyati Binti Mamat, Noorhidayah Candra, Puspa Mega Dahlia Dahlia Damayanti, Ayu Elysia Dayat, Muhammad Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Listyorini Fadhlan Muchlas Abrori Fadilah Fafit Rahmat Aji Fafit Rahmat Aji Fafit Rahmat Aji Fafit Rahmat Aji Fitriani Said Hamdani, Khoirul Haryono Haryono Haryono Haryono Ibrahim Ibrahim Ibrahim Ibrahim Ixora Sartika Mercuriani Kasiman Kasiman Kasiman Kasiman Kasiman, Kasiman Khaleyla, Firas Khoirul Anam KHOIRUL ANAM Kuswanto, Wawan Laila Rasidah H. Lukman Hadi, Lukman M Dayat Mamat, Noorhidayah Binti Matrani Maylina Ilhami Khurniyati Moh. Amin Muhammad Dayat Muhammad Dayat Mulyono Wibisono Mulyono Wibisono Mulyono Wibisono Nawir, Daud Naya Rabbani, Calista Nia Kurniawan Nia Kurniawan Nia Kurniawan Novianti, Nilam Nur Anisa Nur Anisa NUR LINA SAFITRI Nur'aini, Afifah Diyah Nursia Nursia Nursiah Nursiah, Nursiah Panjaitan, Viona Angelina Erlan Parada Agus Pasaribu, Aswin Poetri, Dini Andini Puspa Mega Candra Rabbani, Calista Naya Rahayu, M.Si, Dwi Anggorowati Rahayu, Tanti Rangga, Adam Dwi Rasidah H., Laila Reni Ambarwati Reza Ardiansyah Ribka Ribka Rodiyati Azrianingsih Rodiyati Azrianingsih Rodliyati Azrianingzih Roisatul Ainiyah Rupa, Darius Rusdianto Rusdianto, Rusdianto Salurapa, Adelyn Silfia Ilma Sulung, Cahya Ajeng Valenta Tresna Sunu Kuntjoro Sururi, Ahmad Misbakhus Syarifa Ramadhani Nurbaya Tarmudi, Imam Tresna Sulung, Cahya Ajeng Valenta Trio Ageng Prayitno Umie Lestari Vlorensius Vlorensius Wanandi, Ichsan Bakhtiar Wibisono, Mulyono WIDOWATI BUDIJASTUTI Winarsih Winarsih Yuliawati, Witri Yunita Khilyatun Nisak Yusuf Tomy Harsoyo Zulfadli Zulfadli Zultan, Achmad