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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner ARSHI Veterinary Letters Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Medika Veterinaria

Agustin Indrawati

Bagian Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Fakultas Kedokteran Hewan Institut Pertanian Bogor

Author-ID : 3360648

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Bacterial Pneumonia sebagai Salah Satu Penyebab Kematian Lumba Lumba Hidung Botol Indo-Pasifik (Tursiop aduncus) Agustin Indrawati; Jeni Maharani; Nurul Fadillah; Diah Sekar Arum; Hamdika Yenri; Risqika Aqla Velayati; Ulfi Nurul Fadlilah; Jefri Naldi; Afifah Nurhasanah
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus) merupakan jenis lumba-lumba yang banyak ditemukan di perairan Indonesia. Kelestarian T. aduncus perlu mendapat perhatian karena populasinya yang semakin menurun. Upaya pelestarian yang telah dilakukan adalah melalui konservasi. Gangguan respirasi pada lumba-lumba sering ditemukan dan berakibat fatal, salah satu penyakit yang sering ditemukan adalah pneumonia. Pneumonia jenis bronchopneumonia merupakan salah satu gejala infeksi pada paru yang disebabkan oleh bakteri, virus ataupun jamur. Tujuan penelitian ini adalah melakukan isolasi dan identifikasi bakteri yang berperan dalam kejadian pneumonia. Sampel berasal dari seekor lumba lumba jantan yang ada di Pusat Konservasi Mamalia air. Tahapan penelitian ini antara lain isolasi dan identifikasi bakteri dari organ paru-paru yang menunjukkan gejala patologis. Hasil isolasi dan identifikasi ditemukan 2 spesies Gram positif yaitu Staphylococcus epidermidis dan Staphylococcus aureus serta 4 spesies Gram negatif, yaitu Pseudomonas alcaligenes, Actinobacillus delphinicola, Klebsiella sp. dan Serratia marcesens.

Preparasi Strip Imunokromatografi Koloid Emas untuk Deteksi Cepat Aeromonas hydrophila Suherman .; Retno Damayanti; Agustin Indrawati
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.31-39

Aeromonas hydrophila merupakan agen penyebab Motile Aeromonas Septicemia (MAS) yang menyebabkan kerugian ekonomi pada industri akuakultur air tawar. Saat ini, metode diagnostik yang tersedia untuk determinasi A. hydrophila membutuhkan waktu lama dan tidak sesuai diaplikasikan di lapangan. Oleh karena itu, dibutuhkan adanya metode lain yang dapat digunakan sebagai diagnostik yang cepat dan aplikatif di lapangan. Tujuan dari penelitian ini adalah membuat alat diagnostik yang mampu digunakan sebagai pendeteksi agen penyebab MAS. Secara singkat, partikel koloid emas berukuran 31,88 nm dibuat melalui reduksi kimia asam kloroaurat dengan natrium sitrat. Koloid emas sebagai detektor dikonjugasikan dengan antibodi poliklonal anti-A. hydrophila 50 μg/ml pada pH 7. Membran nitroselulosa sebagai membran reaksi, ditetesi dengan antibodi poliklonal anti-A. hydrophila 2 mg/ml di garis T dan antibodi goat anti-rabbit IgG 1 mg/ml di garis C. Hasil dari penelitian ini, strip imunokromatografi yang dikembangkan mampu mendeteksi A. hydrophila dengan deteksi minimum 1,2x105 CFU/ml. Strip bersifat spesifik terhadap A. hydrophila, tidak ada reaksi silang yang ditemukan ketika direaksikan dengan bakteri lain. Analisis sampel menggunakan strip hanya membutuhkan waktu 10 menit. Strip imunokromatografi yang dikembangkan dapat mendeteksi A. hydrophila dengan cepat dan memiliki potensi untuk digunakan di lapangan.

MP-7 Isolation of Antibiotic Resistance Gene from Staphylococcus aureus from Chicken Poultry Farm in Bogor West Java Indonesia Nabila Swarna Puspa Hermana; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.497 KB)

Staphylococcus aureus is an opportunistic pathogen in human and another animal species. It also associated with staphylococcosis, osteomyelitis, arthritis, and bumble foot disease in poultry. Antibiotics including penicillin, erythromycin and tetracycline are widely used for treating infection caused by S. aureus.Chicken poultry play important role in food supply as one of protein source in Indonesia. West Java province has largest population of chicken poultry, where Bogor has the largest population of chicken poultry with 19.75.850 chicken in 2018. In order to maintain the production, farmers used antibiotics for prophylaxis, growth promotor, and therapy. However, extensive use of antibiotic can be found in low income and developed country, caused the increasing number of antibiotic resistant bacteria.The aim of this study is to determine the antibiotic resistance pattern of S. aureus isolated from cloacal swab of chicken poultry in Bogor West Java Indonesia.

PF-27 Incidence of Feline Dermatophytosis at Dramaga, Bogor in 2013-2018 Agustin Indrawati; Titiek Sunartati; Handina Rakhmawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.126 KB)

Indonesia has a tropical climate and high humidity that give a high prevalence for fungal infections in cat skin such as Feline dermatophytosis (ringworm). Dermatophytosis is the most common fungal infection of cats and one of the most important infectious skin diseases in this species. It may be transmitted to other animal species and also contangious nature, zoonotic potential (Moriello, 2014). Feline dermatophytosis cases are caused by genera Microsporum, Trichophyton, and Epidermophyton. All of these agents produce proteolytic and keratolytic enzymes that enable them to utilize keratin as the sole source of nutrition after colonization of the dead, keratinized portion of epidermal tissue (mostly stratum corneum and hairs, sometimes nails) (Mattei et al., 2014). The aim of this study are to identified and evaluate the kind of fungal that can cause feline dermatofitosis in Dramaga in 2013 until 2018.

Brucella ceti : THE Threat of Emerging Zoonoses in Indo Pacific Bottle Nose (Tursiops aduncus) Agustin Indrawati; Usama Affif
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Incidence of Brucellosis in marine mammals in the world is found lately particularly in Penniped and Cetacea. Indonesia is an archipelago that is rich with its Wildlife and one of them is Indo Pacific bottle nose dolphins (Tursiops aduncus). In this research, Indo Pasific bottle nose dolphins (Tursiops aduncus) were used deriving from captive of marine mammal conservation in Indonesia PT Wersut Seguni. All of them were in good condition. A total of 23 blood samples collected from the caudal vein, located above the tail fin. Blood samples was taken as Aseptic as possible. Screening test against Brucella spp was done by Rose Bengal Test (RBT) and Rapid Test B. Brucella. One sample out of 23 samples had antibody positif against Brucella spp (4%). The results of this research concluded that Brucella spp found is likely to be Brucella Ceti . It is also found in Indonesia territorial and in the Indo Pacific bottle nose dolphins (Tursiops aduncus). We hope that this finding will do raise our awerness to emerging zoonoses.Keywords: brucellosis, Brucella ceti, RBT, Rapid test, Tursiops aduncus

Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis Apris Beniawan; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.25912

Campylobacter fetus subsp. Venerealis (Cfv) is bacteria causing contagious genital diseases in cows called Bovine Genital Campylobacteriosis (BGC) or vibriosis. Isolation of Cfv is difficult, because the bacteria are fragile and need specific nutrients and oxygen (5-10%). The transport media is very important to maintain Cfv survival before culturing in laboratory. The aim of this study was to modify a new transport media as an alternative media for Cfv. Developed media capability was compared to Weybridge media, and Phosphate Buffered Saline (PBS). All transport media was contaminated by Cfv with concentrations of 105,104,103,102,101 (CFU/ml), and was stored for <6, 24, 48, 72, and 96 hours in each transport medium before culturing on blood agar, all in triplicate. The quality of transport media was analyzed based on bacterial growth on blood agar. PCR test was used as a confirmatory test of growing bacteria cultured on blood agar. Based on culture results, Cfv stored in three transport mediums for <6 hours, Cfv grew on blood agar from all concentration levels provided. Cfv stored for 24, 48, 72 and 96 hours on PBS did not grow, whereas on developed media and Weybridge media, the bacteria could grow and enrichment occurs at all concentration levels given. This study indicated the developed media can be used as an alternative transport medium for Cfv bacteria.

Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae) Agustin Indrawati; Damiana Rita Ekastuti; Erdina Pangestika; Reinilda Alwina
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus flavus, Aspergillus fumigatus and Fusarium dimerum.

Faktor Risiko Potensial terhadap Canine Leptospirosis di Ragunan Animal Hospital Jakarta, Indonesia Ambar Retnowati; Agustin Indrawati; Upik Kesumawati Hadi; Safika .; Pratitis S Wibowo; Susan M Noor
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60354

Leptospirosis is a zoonotic disease caused by bacteria Leptospira sp. which causes infection in animals and humans. Dogs infected with leptospirosis showed symptoms such as anorexia, fever, vomiting, weakness, diarrhea and often experience yellowing of the eye area and mucosa around the mouth (icteric) with fatal systemic complications and multi-organ dysfunction, especially in the kidneys and liver. Leptospirosis is an endemic disease in Jakarta. This study aims to identify risk factors that can contribute to canine mortality based on early clinical symptoms that are found when the dog is in an animal health service facility such as a veterinary clinic, veterinary hospital or independent practice veterinarian. Method were used in this study is clinical manifestations and laboratory examinations and medical records of dogs with suspected leptospirosis. Criteria inclusion were based on aspects of the clinical symptoms of dogs in and around Jakarta. Analysis data used the chi-square with confidence of interval (CI) 95%. Dogs used during the study had ages for puppies (less than 1 year) totaling 13 or 32.50%, for adult dogs over 1 year amounted to 27 or 67.50%, 80% male dogs and 20% female. with 80% maintenance system not housed by the owner. Risk factors for clinical symptoms such as myalgia, symptomatic vomiting of the pulmonary area or shortness of breath and abdominal pain, conjunctival suffusion, anorexia and diarrhea contributed to the high mortality rate leptospirosis during study in dogs 2020.

Application of Multiplex PCR Assay for Campylobacter fetus Sub sp. Detection and Differentiation in Bovine Preputial Wash Seruni Agistiana; Surachmi Setiyaningsih; Agustin Indrawati
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i1.26114

The aim of this research was to evaluate the detection and differentiation of C. fetus subspecies through multiplex PCR (mPCR) method from the bovine preputial wash. The evaluation was done by comparing 2 extraction methods, which was by commercial kit extraction and boiling method, and also by measuring the effect of Transport Enrichment Media (TEM) addition as bacterial enrichment. Spiked bovine preputial washes with 2 extraction methods (commercial kit and boiling), with and without TEM addition were used as a sample. The results showed that the highest sensitivity/specificity was by boiling extraction without TEM addition, which reached 83.3%/100% for all or grouped sample. The results also supported by Poisson regression analysis which showed the comparison between sample with and without TEM addition which had 1.3 Incidence Rate Ratio (IRR). The obtained IRR score indicated that sample without TEM addition had 30% better result compared to the sample with TEM addition. Furthermore, a comparison between commercial kit and boiling extraction showed no significant difference (1.029 IRR). The research concluded that boiling extraction without TEM addition is the most accurate, fast and economical extraction method regarding its high sensitivity and specificity value, with less than 24 hours testing time and without any commercial kit requirements. However, a validation and verification with field sample were still needed before applied in the laboratory.

Penggunaan Teknik Molekuler untuk Mengenali Dermatofita yang Diisolasi dari Hewan Kesayangan di Jakarta dan Bogor Dwi Endrawati; Eko Sugeng Pribadi; Agustin Indrawati; Eni Kusumaningtyas
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Dermatophytosis is one of the superficial mycoses which causes skin health problems in pet animals. This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City. Fifty samples of skin scrapings from patients suspected of clinically dermatophytosis were collected and analyzed by conventional and molecular techniques. The Research aimed to identify dermatophyte that were isolated from pet animals using PCR-RFLP technique. The primers of ITS 1, ITS 4, Chytin Synthase, and cutting enzymes of Dde1 were used in this Research. Four specimens off 50 spesimens were tested positive using direct and culture PCR examination techniques. Based on the sequencing results, Microsporum canis was identified in four spesimens. Specimens that were positive for dermatophytes followed by RFLP using the Dde 1 enzyme. The results of the study showed that molecular techniques were a reliable way to determine the high-precision dermatophytes in diagnosing dermatophytosis. The Results also showed that molecular arrangement of B1 isolate was different from three other isolates.

Co-Authors . Darniati Aditya Primawidyawan Afifah Nurhasanah Afiff , Usamah agrita, tri wera Agus Sjahrurachman Ambar Retnowati Ambar Retnowati Andre, Daniel Latief ANGELA MARIANA LUSIASTUTI ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Apris Beniawan Ardiana Ardiana Arum, Diah Sekar Bayu Febram Prasetyo Betty Ernawati Damiana Rita Ekastuti Darniati D, Darniati Denny Widaya Lukman Destri Prameswari, Alvira Diah Nugrahani Pristihadi Diah Sekar Arum Dwi Endrawati Edi Purwanto Elmanaviean Eni Kusumaningtyas Erdina Pangestika Erin Kurnianingtyas Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Ulfi Nurul Fera Ibrabim Guntari Titik Mulyani Hadri Latif Hamdika Yenri Handayani Halik Handina Rakhmawati HELGA SEEGER Hendarko, Sriani Hendry, Altaff Hermana, Nabila Swarna Puspa Herwin Pisestiyani I Wayan T. Wibawan I wayan Teguh Wibawan Idwan Sudirman Ita Krissanti Jefri Naldi Jeni Maharani Julia Rosmaya Riasari Kuntum Khoirani Kurnia Tiara Aulia Lee Xia Meen Leila Nur Aziah Leila Nur Azizah Lila Gardenia Maharani, Jeni Mangaraja Pidoli Tampubolon Mardiastuti Mardiastuti MICHAEL ZSCHÖCK Mira Mawardi Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Muh Ramadhan Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nafiqoh, Nunak Naldi, Jefri Nauval Firdana, Chorrysa Navasuriya Radha Krisnan Nazmi Zahir B. Mohd Zaini Ni Luh Putu Ika Mayasari Ni Luh Putu Ika Mayasari Nining Arini Novita, Hessy Nurhasanah, Afifah Nurul Fadillah Nurul fadillah, Nurul Oktaviani, Dian Okti Nadia Poetri Penataseputro, Tanjung Permana, Nadine Hanifa Pratitis S Wibowo Pratitis S Wibowo Rahman, Muhammad Luthfi Rahmat Hidayat Rahmat Hidayat Ramadhaniah, Vetty Reinilda Alwina Retno D. Soejoedono Retno Damajanti Retno Damayanti Soejoedono Rifky Danial Rifky Rizkiantino Risqika Aqla Velayati Romsyah Maryam Rudramurti, Win Satya Rukmi, M.G. Isworo Ryan Septa Kurnia Safika S, Safika Sapto Andriyono Sartika Juwita Seruni Agistiana Setiadi Setiadi Siti Fatimah Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Sucitya Purnama Suhartila Suhartila Suherman . Surachmi Setiyaningsih Susan M Noor Susan M Noor Susanti, Wiwik Susanto Nugroho Syahidah, Dewi Teguh Budipitojo Titiek Sunartatie Titis Wulandari Tri Wiyoko Trioso Purnawarman Triwardhani Cahyaningsih Ulfi Nurul Fadlilah Uni Purwaningsih Uni Purwaningsih Upik Kesumawati Hadi Usama Affif Usamah Afif Utami, Dian Maulia Uus Saepuloh Velayati, Risqika Aqla Wattiheluw, Muhammad Subhan Yan Evan Yan, Teo Qin Yenri, Hamdika Yuda Heru Fibrianto

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