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Bacterial Identification of Ornamental Betta Fish (Betta splendens) Safika Safika; Nazmi Zahir B. Mohd Zaini; Usamah Afif; Agustin Indrawati; Rahmat Hidayat; Titiek Sunartatie
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.29323

Abstract

Betta fish (Betta splendens) are one of the most popular ornamental fish due to their gorgeous colors and various shapes. This study aimed to find pathogenic bacteria in betta fish (Betta splendens) in fish farm in Bogor. Body swabs were collected from four decorative betta fish, and from tank water. Two of the fish were dead and two of which were exhibiting symptoms of disease. Bacterial isolation was performed on multiple agar media, and bacteria were identified based on the growth characteristics of the colonies, Gram staining, and biochemical testing. The results of the analysis revealed the presence of 16 bacterial isolates, including Edwardsiella sp., Escherichia coli, and Vibrio sp. from dead fish, whereas Citrobacter sp, Providencia sp, and Vibrio sp were detected in diseased fish. In aquarium water, Escherichia coli, Citrobacter sp Proteus sp and Providencia sp. were detected. Betta fish are susceptible to suffer from diseases if the water quality is low since maintaining and enhancing the health of betta fish is highly dependent on water quality.
Study of Risk Level Death Based on Clinical Symptoms in Cases of Canine Leptosprirosis in Jakarta, Depok and South Tangerang: Data from January-August 2020 Ambar Retnowati; Agustin Indrawati; Upik Kesumawati; Safika Safika; Pratitis S Wibowo; Susan M Noor; Uus Saepuloh
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.79400

Abstract

Leptospirosis is a zoonotic disease caused by bacteria Leptospira sp. which causes infection in animals and humans. Dogs infected with leptospirosis showed symptoms such as anorexia, fever, vomiting, weakness, diarrhea and often experience yellowing of the eye area and mucosa around the mouth (icteric) with fatal systemic complications and multi-organ dysfunction, especially in the kidneys and liver. Leptospirosis is an endemic disease in Jakarta. This study aims to identify risk factors that can contribute to canine mortality based on early clinical symptoms that are found when the dog is in an animal health service facility such as a veterinary clinic, veterinary hospital, or independent practice veterinarian. Method was used in this study is clinical manifestations and laboratory examinations and medical records of dogs with suspected leptospirosis. Criteria inclusions were based on aspects of the clinical symptoms of dogs in and around Jakarta. Analysis data used the chi-square with confidence of interval (CI) 95%. Dogs used during the study had ages for puppies (less than 1 year) totaling 13 or 32.50%, for adult dogs over 1 year amounted to 27 or 67.50%, 80% male dogs and 20% female. with 80% maintenance system not housed by the owner. And the conclusion is risk factors for clinical symptoms such as myalgia, symptomatic vomiting of the pulmonary area or shortness of breath and abdominal pain, conjunctival suffusion, anorexia and diarrhea contributed to the high mortality rate leptospirosis during study in dogs 2020.
Deteksi Pola Resistansi dan Gen Penyandi Resistansi Antibiotik pada Escherichia coli Asal Peternakan Babi di Kabupaten Badung Provinsi Bali Ardiana Ardiana; Agustin Indrawati; Ni Luh Putu I Ika Mayasari
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.303

Abstract

Resistansi antimikrob merupakan masalah kesehatan global. Antimikrob termasuk antibiotik digunakan untuk mengobati infeksi pada manusia dan hewan. Penyalahgunaan antibiotik dapat menyebabkan resistansi dan pengobatan menjadi tidak efektif. Penelitian ini bertujuan untuk mengisolasi Escherichia coli (E. coli) dari hewan, manusia dan lingkungan di peternakan babi di Kabupaten Badung Provinsi Bali, serta menentukan pola resistansi dan gen resistansi antibiotik. Sampel diambil dari 12 peternakan babi yang berdekatan dengan total 24 sampel feses babi, 24 sampel swab tangan pekerja kandang, dan 12 sampel limbah air. Escherichia coli diisolasi menggunakan MacConkey Agar, pewarnaan Gram, IMViC (Indole, Methyl Red, Voges Proskauer, Citrate) dan dikonfirmasi dengan Polymerase Chain Reaction (PCR). Uji kerentanan terhadap 11 antibiotik menggunakan metode difusi cakram. Deteksi gen resistansi antibiotik dengan PCR untuk gen blaTEM, ampC, ermB, strA, tetA, dan sul1. Hasil menunjukkan 100% (24/24) E. coli diisolasi dari feses, 16,7% (4/24) dari swab, dan 100% (12/12) dari limbah air. Pola resistansi antibiotik menunjukkan bahwa resistansi tertinggi terhadap eritromisin, diikuti oleh amoksisilin dan ampisilin, tetrasiklin, streptomisin dan trimetropim-sulfmetokzasol, serta 14 isolat dikategorikan Multidrug Resistant (MDR). Gen resistansi yang dideteksi dari E. coli pada manusia, hewan dan lingkungan adalah blaTEM, ampC, strA, tetA dan sul1, sementara ermB tidak terdeteksi. Berdasarkan hasil penelitian, pemberian antibiotik eritromisin, amoksisilin, ampisilin, tetrasiklin, streptomisin dan trimetropim-sulfmetokzasol sebaiknya diganti dengan antibiotik jenis lain. Adanya gen resistansi yang berasal dari sampel feses babi, swab tangan dan limbah air mengindikasikan terjadinya penyebaran resistansi antibiotik pada manusia, hewan dan lingkungan.
Pendekatan One Health: Deteksi Gen blaZ dari Isolat Staphylococcus aureus Asal Peternakan Sapi Perah di Sulawesi Selatan Sartika Juwita; Agustin Indrawati; Retno Damajanti; Safika Safika; Ni Luh Putu Ika Mayasari; Edi Purwanto; Handayani Halik; Muh Ramadhan; Suhartila Suhartila
Jurnal Veteriner Vol 25 No 1 (2024)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2024.25.1.122

Abstract

Resistansi antibiotik merupakan masalah kesehatan penting di seluruh dunia yang berdampak pada kesehatan manusia dan hewan. Kehadiran gen resistan pada Isolat Staphylococcus aureus dapat berkontribusi pada pengembangan resistansi antibiotik. Penelitian ini bertujuan untuk mengidentifikasi keberadaan gen blaZ yang berasal dari isolat S. aureus yang resistan terhadap antibiotik penisilin G dari wilayah peternakan sapi perah di Provinsi Sulawesi Selatan. Sampel penelitian diperoleh dari 20 lokasi peternakan sapi perah yang tersebar di Kabupaten Enrekang. Identifikasi S. aureus dilakukan dengan metode fenotipik dan genotipik. Isolat S. aureus yang resistan terhadap antibiotik penisilin G berasal dari manusia (n=6), ternak sapi perah (n=10), dan produk dangke (n=2) diperoleh dari hasil uji sensitivitas terhadap antibiotik penisilin G (10 U) dengan metode difusi cakram Kirby-Bauer. Deteksi gen blaZ menggunakan metode polymerase chain reaction (PCR) konvensional. Hasil penelitian menunjukkan bahwa isolat yang berasal dari manusia, ternak sapi perah, dan produk dangke terdeteksi keberadaan gen blaZ masing-masing sebesar 100%. Gen blaZ yang terdapat pada isolat manusia, hewan, dan produk dangke berpotensi menyebar antar isolat dan berpotensi menimbulkan gangguan kesehatan manusia.
Lactococcus garvieae: CHARACTERIZATION AND ABILITY TO INHIBIT THE GROWTH OF AQUACULTURE PATHOGENIC BACTERIA Mawardi, Mira; Indrawati, Agustin; Lusiastuti, Angela Mariana; Wibawan, I Wayan Teguh
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.87-98

Abstract

Lactococcus garvieae is a gram-positive ovoid cocci bacterium formerly classified as a member of the Lactococcus genus. This study aims to isolate L. garvieae from catfish rearing pond and characterize it as a potential probiotic candidate. L. garvieae was identified and characterized through phenotypic and genotypic observation, genomic % G~C content analysis, cell surface hydrophobicity assays, acidification test, in vitro antagonism, and a profile of antimicrobial activities. The MT597595.1 accession number corresponds to L. garvieae, as determined by a molecular identification test. Biochemical characterization was performed using API 50 CH kit. The genomics %G~C content of L. garvieae was 51.8. Findings from acidification ability tests, in vitro antagonism tests, and the ability of bacteria to grow in broth medium at pH 4 reveal that L. garvieae can inhibit the growth of Aeromonas hydrophila, Streptococcus agalactiae, Streptococcus iniae, and Edwardsiella ictaluri. However, it does not suppress the growth of L. garvieae  Edwardsiella tarda. Remarkably, L. garvieae has the ability to reduce the pH of neutral broth medium turning it acidic. Furthermore, L. garvieae’s hydrophobic cell surface exhibited an adhesive, hydrophobic, and protein surface cell content with a compact growth pattern consistent with postive SAT and MATH assay. Antimicrobial activity tests, encompassing 11 antibiotics, disclosed resistance to  Nalidixic acid while displaying intermediate sensitivity to Streptomycin and Trimethoprim. In conclusion, L. garvieae demonstrates an inhibitory effect on the growth of pathogenic bacteria, underlining its potential as a probiotic candidate.
CHARACTERIZATION OF SPORE-FORMING BACTERIA ISOLATED FROM TILAPIA (OREOCHROMIS NILOTICUS) AND THEIR POTENTIAL FOR A PROBIOTIC CANDIDATE Mawardi, Mira; Indrawati, Agustin; Lusiastuti, Angela Mariana; Wibawan, I Wayan Teguh
Indonesian Aquaculture Journal Vol 18, No 2 (2023): (December, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.2.2023.105-114

Abstract

Gram-positive spore bacteria are widely used as probiotics in general sectors. However, there are still limited bacterial isolates as probiotic candidates available from indigenous isolates, especially in aquaculture. This study aimed to obtain potential spore-forming isolates as probiotic candidate for tilapia. Tilapia fish samples were collected from Sukabumi, Ciamis, Serang, and Papua. Bacterial isolates were isolated from the digestive tract of tilapia. Bacteria were identified based on their morphological, molecular characteristics, complete genome composition, and cell surface identification based on hydrophobic properties. In this study, six bacteria were isolated and identified by molecular characteristics using 16S rRNA sequences. Based on the phylogenetic analysis, the 9 PP isolate was Priestia megaterium basonym: Bacillus megaterium, CMS 16N isolate was Brevibacillus halotolerans, PPN 10 isolate was Bacillus sp., 3.1 SKBM isolate was Bacillus mycoides, CMS 22 N and SRG32 isolate were Bacillus subtilis. Six bacteria had different phenotypicals, ATGC sequence compositions, and a higher proportion of total G~C sequence composition above 50%. The coherent cell surface hydrophobicity test was positive on the SAT, SA, AA, and compact growth patterns in soft-agar media for 9 PP, CMS 22 N, and SRG32 isolates. From our study, the indigenous spore-forming bacteria isolated from tilapia stomachs are enzymatic bacteria, which have a strong attachment to host tissue and high potential as a probiotic candidate for fish. Various hydrophobicity test results from each isolate indicate that the protein composition in the cell surface is different.
Uji Resistansi Antibiotik Bakteri Vibrio Parahaemolyticus dari Udang Putih (Litopaneus Vannamei) Serta Identifikasi Gen Penyandi Resistan Ampisilin Ramadhaniah, Vetty; Agustin Indrawati; Bayu Febram Prasetyo
Acta VETERINARIA Indonesiana Vol. 12 No. 2 (2024): Juli 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.2.97-105

Abstract

Penyakit vibriosis pada udang putih (Litopaneus vannamei) sangat ditakuti oleh pembudidaya pada tahun 2009 karena diduga dapat menimbulkan early mortality syndrome (EMS). Penyakit ini menyebabkan kematian massal pada usia muda. Umumnya vibriosis pada udang dicegah dan diobati dengan menggunakan antibiotik. Penggunaan antibiotik yang tidak tepat dapat menyebabkan resistansi multi-antibiotik. Penelitian ini dilakukan untuk mengetahui tingkat resistansi antibiotik terhadap bakteri Vibrio parahaemolyticus di udang putih dan mengidentifikasi keberadaan gen resistan terhadap ampisilin. Isolat bakteri V. parahaemolyticus diuji resistansi terhadap antibiotik ampisilin, oksitetrasiklin, kloramfenikol, enrofloksasin, dan eritromisin. Penelitian ini dilakukan menggunakan metode difusi cakram Kirby-Bauer dengan tabel Clinical and Laboratory Standards Institute (CLSI). Hasil uji resistansi menunjukkan bahwa 30 isolat V. parahemolyticus sensitif terhadap enrofloxacin (94%), dan kloramfenikol (97%). Adapun hasil uji terhadap ampisilin menunjukkan adanaya resistan (77%). Bakteri yang resistan terhadap ampisilin diuji terhadap keberadaan gen yang mengkode protein BlaTEM yaitu dengan hasil persentase 100% (dari 6 sampel) pada amplikon 516 bp. Dapat disimpulkan bahwa isolat V. parahaemolyticus resistan terhadap ampisilin terhadap gen BlaTEM.
Identifikasi Bakteri Pencernaan dan Uji Resistansi pada Primata di Kebun Binatang Bukittinggi safika, safika; Indrawati, Agustin; Hidayat, Rahmat; Afiff, Usamah; Sunartatie, Titiek; Nauval Firdana, Chorrysa; Destri Prameswari, Alvira
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.196-203

Abstract

Bakteri merupakan satu di antara penyebab terjadinya beberapa penyakit infeksi. Jenis bakteri yang dapat menginfeksi tubuh berbeda-beda tergantung organ atau lokasi target. Organ yang sering diinfeksi oleh bakteri adalah saluran pencernaan. Penelitian ini bertujuan mengidentifikasi jenis bakteri saluran pencernaan dan pola resistansi bakteri terhadap beberapa jenis antibiotik. Feses primata diperoleh dari Kebun Binatang Bukittinggi yang diisolasi pada media Eosin Methylene Blue Agar (EMBA), MacConkey agar (MCA), dan agar darah. Isolat bakteri yang didapat kemudian diuji dengan pewarnaan Gram, dan uji biokimia untuk diidentifikasi. Bakteri Escherichia coli dan Staphylococcus aureus yang didapat dilakukan uji resistansi dengan metode disk Kirby Bauer. Jenis bakteri Gram negatif yang dapat diidentifikasi yaitu Shigella sp., Proteus sp., Escherichia coli, Klebsiella oxytoca, dan Yersinia sp., serta bakteri Gram positif yaitu Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus sp., Stomatococcus mucilaginosus, dan Bacillus sp.. Bakteri E. coli mengalami resistan terhadap antibiotik ampisilin, streptomisin, eritromisin (2 isolat dari 2 isolat), amoksisilin tetrasiklin, oksitetrasiklin, doksisiklin, gentamisin, kloramfenikol dan asam nalidiksat (1 isolat dari 2 isolat), sedangkan bakteri S. aureus hanya mengalami resistansi terhadap antibiotik ampisilin dan amoksisilin (1 isolat dari 1 isolat). Resistansi tersebut dapat terjadi karena penggunaan antibiotik yang tidak tepat, perpindahan gen antarmikroorganisme.
Isolasi dan Identifikasi Aeromonas hydrophila pada Ikan Lele (Clarias gariepinus) Pertambakan Muara Jambi, Provinsi Jambi Wulandari, Titis; Indrawati, Agustin; Pasaribu, Fachriyan Hasmi
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.612 KB) | DOI: 10.20473/jmv.vol2.iss2.2019.89-95

Abstract

Tujuan penelitian ini adalah untuk mengidentifikasi bakteri Aeromonas hydrophila pada ikan lele dengan gejala klinis pada kolam bioflok di Kabupaten Muara Jambi, Provinsi Jambi. Bakteri diisolasi dari 35 ekor ikan lele yang memiliki gejala infeksi A. hydrophila. Pengambilan sampel dilakukan Purposive random sampling. Identifikasi dilakukan uji biokimia berdasarkan Standar Nasional Indonesia SNI 7303.1-2015 dan dilakukan uji konfirmasi metode PCR taget gen aerA. Hasil penelitian menujukkan bahwa sebanyak 35 ekor ikan terisolasi 12 isolat bakteri A. hydrophila pada uji biokimia. Namun pada pengujian PCR pada agarose gel 0.8% menujukkan 8 Isolat posistif gen aerA pada 309 bp. Hasil yang berbeda dapat diakibatkan karena adanya positif palsu pada saat uji biokimia. Hasil identifikasi A. hydrophila dengan metode PCR dapat digunakan hasil yang lebih akurat dan waktu uji lebih cepat dibandingkan dengan uji biokimia. Identifikasi yang cepat dapat mempermudah dalam pencegahan infeksi lebih efektif dan cepat.
Isolation and Identification of Vibrio parahaemolyticus Bacteria in Bottlenose Dolphins (Tursiop truncates) in Kendal Conservation Pond, Central Java Nafiqoh, Nunak; Setiadi, Setiadi; Novita, Hessy; Lusiastuti, Angela Mariana; Indrawati, Agustin; Elmanaviean; Jannah, Siti Nur; Gardenia, Lila; Penataseputro, Tanjung; Andriyono, Sapto; Ningrum, Siti Gusti; Oktaviani, Dian; Syahidah, Dewi; Wattiheluw, Muhammad Subhan; Purwaningsih, Uni
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.811-819

Abstract

Aquatic mammals in Indonesia are officially protected. However, there is a lack of research on these mammals, particularly in relation to potential disease-causing bacteria. A study was conducted in a conservation pond to address this gap, where swab samples were taken from the blowholes of aquatic mammals. The focus was on identifying bacteria that could potentially cause infectious diseases in these animals. The results revealed V. parahaemolyticus bacterial isolates, which showed a 98% similarity to the registered V. parahaemolyticus in NCBI. These bacterial isolates exhibited hemolysin properties and demonstrated resistance to trimethoprim, streptomycin, cephalothin, and penicillin antibiotics.
Co-Authors . Darniati Aditya Primawidyawan Afifah Nurhasanah Afiff , Usamah Agus Sjahrurachman Ambar Retnowati Ambar Retnowati Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI ANGELA MARIANA LUSIASTUTI Apris Beniawan Ardiana Ardiana Arum, Diah Sekar Bayu Febram Prasetyo Betty Ernawati Damiana Rita Ekastuti Denny Widaya Lukman Destri Prameswari, Alvira Diah Sekar Arum Dwi Endrawati Edi Purwanto Elmanaviean Eni Kusumaningtyas Erdina Pangestika Erin Kurnianingtyas Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Ulfi Nurul Fera Ibrabim Guntari Titik Mulyani Hadri Latif Hamdika Yenri Handayani Halik Handina Rakhmawati HELGA SEEGER Hendarko, Sriani Herwin Pisestiyani I Wayan T. Wibawan I wayan Teguh Wibawan Idwan Sudirman Ita Krissanti Jefri Naldi Jeni Maharani Julia Rosmaya Riasari Kuntum Khoirani Kurnia Tiara Aulia Lee Xia Meen Leila Nur Aziah Leila Nur Azizah Lila Gardenia Maharani, Jeni Mangaraja Pidoli Tampubolon Mardiastuti Mardiastuti MICHAEL ZSCHÖCK Mira Mawardi Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Muh Ramadhan Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nabila Swarna Puspa Hermana Nafiqoh, Nunak Naldi, Jefri Nauval Firdana, Chorrysa Navasuriya Radha Krisnan Nazmi Zahir B. Mohd Zaini Ni Luh Putu Ika Mayasari Ni Luh Putu Ika Mayasari Nining Arini Novita, Hessy Nurhasanah, Afifah Nurul Fadillah Nurul fadillah, Nurul Oktaviani, Dian Okti Nadia Poetri Penataseputro, Tanjung Pratitis S Wibowo Pratitis S Wibowo Rahmat Hidayat Rahmat Hidayat Ramadhaniah, Vetty Reinilda Alwina Retno D. Soejoedono Retno Damajanti Retno Damayanti Soejoedono Rifky Danial Rifky Rizkiantino Risqika Aqla Velayati Romsyah Maryam Rukmi, M.G. Isworo Ryan Septa Kurnia Safika S, Safika Sapto Andriyono Sartika Juwita Seruni Agistiana Setiadi Setiadi Siti Fatimah Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Sucitya Purnama Suhartila Suhartila Suherman . Surachmi Setiyaningsih Susan M Noor Susan M Noor Susanti, Wiwik Susanto Nugroho Syahidah, Dewi Teguh Budipitojo Titiek Sunartatie Titis Wulandari Tri Wiyoko Trioso Purnawarman Triwardhani Cahyaningsih Ulfi Nurul Fadlilah Uni Purwaningsih Uni Purwaningsih Upik Kesumawati Hadi Usama Affif Usamah Afif Uus Saepuloh Velayati, Risqika Aqla Wattiheluw, Muhammad Subhan Yan Evan Yenri, Hamdika Yuda Heru Fibrianto