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Keragaman Fisikokimia Beberapa Populasi Tanaman Garut di Kabupaten Jember Kacung Hariyono; Vega Kartika Sari; Riza Yuli Rusdiana; Indri Fariroh; Widya Kristiyanti Putri; Didik Pudji Restanto
Vegetalika Vol 12, No 1 (2023): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.78023

Abstract

Garut (Maranta arundinacea L.) merupakan tanaman perenial yang umbinya dapat dimanfaatkan sebagai sumber pangan alternatif, pakan, dan bahan baku industri. Tanaman garut di Kabupaten Jember masih sebagai tanaman liar. Penelitian ini bertujuan untuk mengetahui keragaman fisikokimia garut dari beberapa kecamatan di Kabupaten Jember. Variabel fisikokimia yang diamati meliputi kadar air, sukrosa, protein terlarut, antioksidan, flavonoid, phenolic, gula reduksi, dan karbohidrat. Data yang diperoleh dianalisis korelasi dan biplot. Hasil korelasi menunjukkan semakin tinggi kadar air maka semakin rendah kandungan sukrosa pada umbi garut (r= -0.480), juga dengan kandungan protein terlarut (r=-0.515) dan gula reduksi (r=-0.698). Semakin tinggi kandungan sukrosa pada umbi garut maka semakin tinggi pula kandungan protein terlarut (r=0.515), phenolik (r=0.513) dan gula reduksi (r=0.657). Berdasarkan hasil analisis biplot, total keragaman fisikokimia garut di Kabupaten Jember sebesar 58.6%. Kandungan flavonoid memiliki nilai keragaman kecil dibanding variabel lainnya. Umbi garut yang diperoleh dari Kecamatan Wuluhan (Desa Tanjangrejo dan Glundengan) memiliki kandungan gula reduksi dan protein terlarut rendah, dan umbi garut dari Kecamatan Sumbersari (Desa Kranjingan) memiliki kandungan antioksidan dan karbohidrat paling tinggi.
Somatic Embryogenesis Direct And Indirect On Porang Plants (Amarpopallus oncophilus) Mohammad Nur Khozin; Didik Pudji Restanto; Dwi Erwin Kusbianto
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol. 7 No. 2 (2022): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v7i2.6053

Abstract

ABSTRACT Propagation of conjact using somatic embryogenesis techniques either directly or indirectly gives hope procuring large quantities of seeds. This study aims to evaluate the method of direct and indirect somatic induction of embryogenesis from conjact. In vitro porang leaves were used as explants. The media used is Murashige and Skoog (MS) media with a combination of NAA and BAP. The results showed that in vitro porang leaf explants cultured in media containing BA 2.22 M could induce embryosomatics directly, whereas media containing single 20 M NAA and a combination of NAA 20 M + BA 2.22 M induced direct and or indirect embryosomatics. . The best medium for induction was shown by 20 M NAA media which had 100% SE induction percentage and fast time.relatively. Keywords: Conjact, somatic embryogenesis, hormones, direct, indirect.
Induksi dan Kultur Suspensi Friable Embryogenic Callus Ubi Kayu Menggunakan Kultivar Lokal dan Jenis Auksin yang Berbeda Endah Cahyani Simamora; Slameto Slameto; Didik Pudji Restanto
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1535

Abstract

Tanaman ubi kayu (Manihot Esculenta) merupakan komoditas yang diprioritaskan serta mudah untuk dibudidayakan. Perakitan tanaman ubi kayu memiliki kesulitan dikembangkan secara generatif sehingga dibutuhkan pendekatan kultur jaringan melalui induksi Friable Embryogenic Callus. Beberapa kendala dalam menghasilkan kalus yang embriogenik yaitu genotip serta eksplorasi dari metode dan protokol induksi FEC dari beberapa varietas ubi kayu masih terbatas. Penelitian dilakukan dengan beberapa tahapan yaitu induksi FEC pada media GD dengan perlakuan kultivar yang berbeda yaitu Kaspro (V1), Ketan (V2), Kuning (V3) pada media GD, dilanjutkan dengan proliferasi pada kultur suspensi dengan perlakuan jenis auksin yaitu picloram 12 mg.L-1 (P1), 20 mg.L-1 (P1), dan 2,4-D 12 mg.L-1 (P3) dan perbedaan kultivar. Hasil penelitian yang diperoleh yaitu respon kalus dari kultivar lokal menunjukkan rerata frekuensi dan skor FEC tertinggi terdapat pada Kultivar Kaspro sebesar 51,26% dan skor 4 sebagai kategori kemampuan membentuk FEC tinggi. Pertambahan berat segar tertinggi terdapat pada perlakuan P1 yaitu 1,19 gram serta perlakuan V2P1 yaitu 0,64 gram. Volume suspensi tertinggi terdapat pada perlakuan V1P2 serta viabilitas sel tertinggi terdapat pada perlakuan V3P2.TRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian //  TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//
Pendampingan masyarakat untuk pembentukan TABOGA Farm guna peningkatan kesejahteraan petani di desa Klangon kabupaten Madiun Fariz Kustiawan Alfarisy; Didik Pudji Restanto; Laily Ilman Widuri; Yuli Witono; Fifteen Aprila Fajrin; Hadi Paramu
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 8, No 2 (2024): June
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v8i2.22740

Abstract

AbstrakDesa Klangon saat ini menjadi desa rujukan tanaman porang Indonesia. Tidak hanya itu Desa Klangon memiliki wisata klangon yang terletak di tengah hutan klangon. Tujuan dari pengabdian masyarakat adalah memberikan pemberdayaan dan pendampingan masyarakat mengenai implementasi Agro Smart Village sebagai solusi pengelolaan wisata dan TABOGA dalam upaya peningkatan kemandirian dan derajat kesehatan masyarakat. Tahapan pelaksanaan kegiatan meliputi koordinasi dengan perangkat desa,  FGD (Focus Group Discussion) dan praktek di beberapa kelompok masyarakat, pendampingan, pembentukan TABOGA farm, dan pendampingan, Pendampingan dalam perencanaan pengembangan usaha hilirisasi produk TABOGA. Kegiatan pengabdian desa Asal melibatkan beberapa perangkat desa, tokoh masyarakat, dan petani yang menjadi sasaran. Hasil yang didapatkan dari kegiatan pengabdian ini diantaranya adalah komoditas TABOGA di desa klangon memiliki potensi yang besar untuk dikembangkan sebagai komoditas unggulan kedua setelah porang. Setidaknya dari hasil FGD tercatat sebanyak 18 jenis tanaman obat tradisional yang banyak tumbuh berdampingan dengan tanaman porang di hutan Desa Klangon. Namun, potensi ini belum banyak dimanfaatkan secara maksimal. Permasalahan utama yang menjadi kendala adalah pemasaran produk TABOGA. Berdasarkan permasalahan tersebut, kontribusi yang diberikan oleh Tim Pengabdi pada kegiatan ini yakni  pendampingan implementasi Agro Smart Village melalui pemetaan komoditas TABOGA untuk meningkatkan minat masyarakat dalam mengembangkan komoditas TABOGA untuk kesejahteraan masyarakat Desa Klangon. Kata kunci: pemasaran; porang; tanaman obat tradisional; wisata. AbstractKlangon Village is currently a reference village for Indonesian porang plants. Klangon Village has a Klangon tourist area which is in the middle of the Klangon forest. The purpose of community service was to provide community empowerment and assistance regarding the implementation of Agro Smart Village as a tourism management solution and TABOGA to increase self-sufficiency and community health status. The method of implementing the assisted village service activities was carried out using two methods, namely FGD (Focus Group Discussion) and practiced in several community groups. The production technology practice method aimed to aid the community regarding the formation of TABOGA Farm. Origin village service activities involved several village officials, community leaders, and targeted farmers. Based on the results of the community service activities, the Klangon people required assistance from universities to make mapping of TABOGA commodities and their marketing. Farmers need institutional support such as BUMDES to become a business unit that accommodates community taboga plant products so that it is hoped that they can increase people's income.Keywords: tourism; traditional medicinal plant; marketing.
Organogenesis formation Porang plant (Amorphophallusmuelleri B.) At Several Concentrations of TDZ (Thidiazuron) Didik Pudji Restanto; Saputro Gumelar, Bachtiar; Handoyo, Tri; Ubaidillah, Mohammad; Candra Prayoga, Mohammad
Jurnal Penelitian Pertanian Terapan Vol 24 No 1 (2024)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v24i1.2882

Abstract

The porang plant (Amorphophallus muelleri B.) is a tuber plant. Porang plant propagation still uses conventional methods by using seeds or frogs that experience dormancy and take a long time to make seeds. The tissue culture technology approach through organogenesis is a solution for the supply of large-scale porang seed material. The research aims to determine the effect of TDZ (Thidiazuron) on the formation of organogenesis. This study used porang leaf explants grown on MS media. The research design used a completely randomized design with one factor, namely TDZ hormone at concentrations of 0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, and 4 mg/L. The results showed that leaf explants on the addition of TDZ hormone could form organogenesis indirectly, but gave a response to form shoots multiplication. TDZ concentration of 2 mg/L gave the highest proportion value in the parameter of callus formation power of 95%, regeneration power of 88%, and  number of shoots of 10.2 shoots.  
Study of nitrogen status in different planting systems and organic fertilizer doses in sandy soil Soeparjono, Sigit; Bintoro, Jatmiko Hardi; Khozin, Mohammad Nur; Putri, Widya Kristiyanti; Restanto, Didik Pudji
Journal of Degraded and Mining Lands Management Vol. 12 No. 1 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.121.6789

Abstract

This study was conducted to determine the effect of different jajar legowo planting systems in intercropping sweet corn with peanuts, as well as the addition of several doses of organic fertilizer on soil nitrogen status and plant leaf tissue on sandy soil. The research was conducted from January to May 2023, on the south coast of Jember Regency. The study used a split-plot design; the main plot consisted of the treatment of 2:2 (A1) and 4:2 (A2) jajar legowo intercropping systems. The subplots were different doses of cow dung organic fertilizer: 10 t ha-1 (B1), 20 t ha-1 (B2), and 30 t ha-1 (B3), each repeated three times. Observational data were analyzed by ANOVA and DMRT 5%. The results of the study showed that the combination of the planting system and organic fertilizer doses applied had not been able to provide optimal microclimate and soil properties for the growth and development of sweet corn plants. The A2 planting system provides a better microclimate and plant morphology for plant N availability and absorption. The addition of organic fertilizer up to the highest dose (B3) has not been able to maintain optimal soil water and N availability for sweet corn in sandy soil.
The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B) Restanto, Didik Pudji; Aji, Seto Purnomo; Handayani, Etty; Ratnasari, Tri; Jadmiko, Mochammad Wildan; Prayoga, Mohammad Candra; Khozin, Mohammad Nur; Kriswanto, Budi
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18063

Abstract

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).
Artificial Neural Network Performance on Pakcoy Leaf Fresh Weight Model Rusdiana, Riza Yuli; Widuri, Laily Ilman; Restanto, Didik Pudji
Jurnal ILMU DASAR Vol 23 No 2 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i2.27283

Abstract

The growth of leaf biomass can be predicted from an increase in the surface area and thickness of the leaves. Measurements of leaf biomass are approached with the fresh weight of the leaves. The relationship between biomass and leaf surface area commonly performed by regression analysis. The analysis requires assuming linear relationship between dependent variables and independent variables. Artificial Neural Network (ANN) is alternative that can be used to analyze the relationship of leaves and leaf biomass without requiring linear relationships. The research aimed to evaluate ANN performance in determining the fresh weight of pakcoy leaves based on leaf area parameters. Datasets in the study included leaf area datasets and length-width datasets. ANN architecture used Multi Layer Perceptron (MLP) with backpropagation. Ramsey’s test results showed that leaf area datasets is linier model and length-width datasets is nonlinier model. ANN performs well in predicting leaf fresh weight data on both nonlinear and linear models. The best ANN architecture for modeling the leaf fresh weight with leaf area is MLP (1-3-1) while the leaf fresh weight model with length and width is MLP (2-3-1).
Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang) Azizah, Kunti Anis; Restanto, Didik Pudji; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1379.154 KB) | DOI: 10.19184/jid.v18i2.2592

Abstract

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus
SOSIALISASI PENGEMBANGAN KAMPUNG HORTIKULTURA DI KABUPATEN PROBOLINGGO DAN LUMAJANG Soeparjono, Sigit; Usmadi, Usmadi; Harsanti, Restiani Sih; Dewanti, Parawita; Restanto, Didik Pudji; Putri, Widya Kristiyanti
PAPUMA: Journal of Community Services Vol. 1 No. 01 (2023): November 2023
Publisher : Program Studi Agronomi Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/papuma.v1i01.638

Abstract

Probolinggo and Lumajang districts are two regions in East Java that have horticultural villages with the potential to develop fruit and vegetable crops. Efforts to develop horticultural villages in the region need to be made to achieve the realization of horticultural villages that are independent and advanced, more modern and have high competitiveness. This socialization and development activity aims to increase understanding of horticultural farmer groups regarding efforts to develop horticultural villages in both regions in accordance with the standards of horticultural villages that are independent and advanced and have sales value, provide guidance and training to horticultural village farmers regarding the cultivation of horticultural plants and processing of products accordingly. commodities developed in each region, explore several factors that influence the level of success and sustainability of the horticultural village development program in the sampling area, obtain input regarding recommendations for improving programs or activities carried out in each sampling horticultural village using descriptive and quantitative analysis . Horticultural farmers' understanding regarding the development of horticultural villages in the Probolinggo and Lumajang districts is still low and does not meet the standards of horticultural villages that are independent and advanced and have sales value. Efforts to develop horticultural villages in the Probolinggo and Lumajang districts show positive developments in several commodities, while for annual fruit commodities, factors that influence the sustainability indicators of the horticultural village development program. Apart from that, there are factors such as the quality of the farmer group's human resources and the involvement of stakeholders which greatly influences the sustainability of the development of horticulture villages. Efforts to develop horticulture villages require the provision of facilities and assistance with production facilities, infrastructure and guidance on post-harvest handling, processing, market development and the development of farmers' economic institutions.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Endah Cahyani Simamora Farlisa, Veronenci Yuliarbi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mohammad Nur Khozin Mohammad Nur Khozin Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo TRI HANDOYO Tri Handoyo Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz