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IDENTIFIKASI SENYAWA ANTIOKSIDAN DALAM FRAKSI METANOL DAUN BIDARA (Ziziphus mauritiana) Sriwijayanti, Sriwijayanti; Nasiro, Siti; Humesi, Lusi; Situmeang, Boima; Puspitasari, Puspitasari
Arfak Chem: Chemistry Education Journal Vol. 6 No. 2 (2023): Arfak Chem
Publisher : Universitas Papua, Manokwari, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/accej.v6i2.525

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Tumbuhan Bidara (Ziziphus mauritiana) merupakan salah satu tumbuhan yang dimanfaatkan oleh masyarakat untuk mengobati berbagai penyakit. Bidara  tumbuh didaerah yang kering dengan curah hujan yang sangat rendah. Di Indonesia, tumbuhan bidara banyak ditemukan didaerah Banten, Jawa, Madura, Bali, Sawu, Rote, Timur leste, Alor, Makasar, Bima, dan Sumba. Pada penelitian sebelumnya, ekstrak daun bidara diketahui memiliki aktivitas sebagai antioksidan. Penelitian ini bertujuan untuk mengisolasi senyawa bioaktif dan menguji aktivitas antioksidan pada fraksi metanol daun bidara. Ekstraksi dilakukan menggunakan metode maserasi dengan pelarut metanol. Pemisahan dan pemurnian senyawa menggunakan metode kromatografi. Uji aktivitas antioksidan menggunakan metode DPPH (1,1-difenil-2-pikrihidrazin). Karakterisasi dan elusidasi struktur isolat yang mempunyai nilai % inhibisi tinggi dilakukan dengan menggunakan spektroskopi UV dan FTIR. Berdasarkan hasil uji aktivitas antioksidan fraksi 10 menghasilkan nilai % inhibisi yang tinggi yang dapat meredam radikal bebas. Hasil elusidasi struktur, senyawa yang diperoleh merupakan senyawa flavonoid.
Hubungan Profil Lipid Dengan Kadar Glukosa Darah Pada Pasien Diabetes Mellitus: Relationship between Lipid Profile and Blood Glucose Levels in Diabetes Mellitus Patients Puspitasari, Puspitasari; Aliviameita, Andika
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 1 No. 2 (2018): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i2.1831

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Diabetes mellitus is disease caused by a disorder of glucose metabolism. At present, diabetes mellitus is health problem in the world. Increased blood glucose levels can increase the risk of complications of various organs, one of which is cardiovascular disease. This study aims to determine the relationship between cholesterol total levels and triglycerides with blood glucose levels in patients with diabetes mellitus. This type of research is observational analytic with cross sectional design. A total of 45 samples were selected selectively at Aisyiyah Siti Fatimah Tulangan Hospital in January 2016-February 2018. The results of multiple linear regression analysis showed a statistically significant relationship between total cholesterol levels and blood glucose (b = 0,607; p = 0,046), and triglyceride levels with blood glucose levels (b = -0,276; p = 0,003). Based on the results, we found a stati
Pengaruh Infusa Rimpang Temu Putih (Curcuma zedoaria (Berg.) Roscoe) Terhadap Jumlah Leukosit Dan Differential Counting (Diffcount) Pada Kesembuhan Luka Laparatomi Pasca Bedah: Effect of Infusion of Temu Putih Rhizome (Curcuma zedoaria (Berg.) Roscoe) on Leukocyte Count and Differential Counting (Diffcount) on Postoperative Laparotomy Wound Healing Wulandari, Ratna; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1689

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Laparatomy is surgery performed to open the abdominal area which causes an increase in plasma adrenaline and inflammatory. Leukocytes will increase if the adrenal glands are stimulated as a physiological response such as stress and when active against microorganisms. Leukocytosis can trigger an increase in the number of neutrophil and lymphocyte cells in the blood circulation and cause an increase in leukocytes and diffcount. Curcuma zedoaria contain curcumin compounds as antioxidant effectiveness to prevent fisiologis stress, flavonoid and essential oils as an anti-inflammatory which can increase the total of leukocyte. The objective of this research is to determine the effect of several Curcuma zedoaria infusion on the total of leukocytes and diffcount on post operative laparatomy wound healing. A total of 24 Rattus norvegicus were used in this research. Group I (without infusion); group II (10% infusion); group III (15% infusion); group IV (20% infusion). Rattus norvegicus were sreened laparatomy and their blood was taken for leukocyte and differential counting. There is an effect of Curcuma zedoaria infusion on the number of leukocytes and diffcount. The best concentration 15% of leukocyte, and diffcount such as concentration 20% granulocyte, concentration 15% lymphocytes and concentration 10% monocyte in postoperative laparatomy wound healing.
Pengaruh Ekstrak Daun Kedondong (Spondias dulcis)Terhadap Pertumbuhan Trichophyton rubrum Secara In Vitro: Effect of Kedondong (Spondias dulcis) Leaf Extract on In Vitro Growth of Trichophyton rubrum Nirosa, Givari Dwi; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1691

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Dermatophytes are fungi that can cause dermatophytosis, this fungus attacks the skin of the stratum corneum. This study aims to determine the effectiveness Spondias dulcis leaf extract on the growth of Trichophyton rubrum in vitro. This research uses Trichophyton rubrum and Spondias dulcis leaf extract. The treatment in this study was divided into 6 treatment groups, namely negative control, concentration of 20%, 40%, 80%, 100% and positive control, as well as 4 repetitions. After incubation for 5-7 days then observations were made and the results showed that Spondias dulcis leaf extract with various concentrations did not have different results, there was no effect on the growth of Trichophyton rubrum in vitro. Negative control used 10% DMSO solution which showed no effect on the growth of Trichophyton rubrum. On positive control using terbinafine antifungal drugs showed results that were very influential on the growth of Trichophyton rubrum. So it can be concluded that Spondias dulcis leaf extract has no influence on the growth of Trichophyton rubrum.
Difference between Uric Acid and Albumin Levels in Chronic Kidney Failure Patients Before and After Hemodialysis : Perbedaan Kadar Asam Urat dan Albumin Pada Pasien Gagal Ginjal Kronik Sebelum dan Sesudah Hemodialisis Maha, Maha; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 1 (2022): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i1.1623

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Chronic kidney failure (CKF) is a decrease in kidney function has decreased. The impact that occurs is the accumulation of metabolic waste, including fluid overload, uremia, hyperuricemia and hyperkalemia. Hemodialysis is the process of cleansing the blood through a distillation process outside the body using a machine (dialyzer). The purpose of this study was to determine the differences in uric acid levels and albumin levels in patients with chronic renal failure before and after hemodialysis at Dr. Muhammad Zyn Sampang General Hospital. This type of research is experimental with 15 patients using purposive random sampling technique. The method of examining uric acid using Enzymatic Photometry, and the method of examining albumin using Bromocresol Green. The research was conducted in April-May 2021 at RSUD Dr. Muhammad Zyn Sampang. This study used the Wilcoxon test. It can be concluded that there is a significant difference (p <0.05) uric acid levels (p = 0.001) and albumin levels (p = 0.001) in patients with chronic renal failure before and after hemodialysis.
Relationship Between Blood Glucose Levels With Albumin and HDL (High-Density Lipoprotein) Levels In Diabetic Ulcers: Hubungan Kadar Glukosa Darah Terhadap Kadar Albumin dan Kadar Kolesterol HDL (High-Density Lipoprotein) Pada Pasien Ulkus Diabetikum Ilmi, Isyfa'ul; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i2.1658

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Diabetes mellitus is a disease caused by disorders of the body’s metabolism, because blood glucose levels have a increased or hyperglycemia. According to the IDF in 2021 DM sufferers have increased from 463 million people (9.3%) to 537 million people (10.5%) in 2021. The purpose of this study was to determine the relationship between blood glucose levels and blood albumin and HDL cholesterol in diabetic ulcer patients. The design of this study was a cross-sectional method, in 30 respondents with diabetes mellitus with diabetic ulcers who were undergoing treatment at the Sidoarjo Luka Home. Examination of glucose, albumin and HDL cholesterol was carried out using the enzymatic method using a photometer, on 28 May – 20 June 2022. The results of the study were analyzed using Pearson’s Parametric Correlation statistics. Blood glucose levels with blood albumin levels have a strong correlation with a negative correlation direction (r = -0.470 p= 0.009), while blood glucose levels with HDL (High-Density Lipoprotein) cholesterol levels have a weak correlation with a negative correlation direction (r=-0.375 p=0.041).
The Effect Of Delay Time And Storage Temperature On The Results Of Urine Leukocyte Examination In Patients Of Urinary Tract Infection (UTI): Pengaruh Lama Waktu Penundaan Dan Suhu Penyimpanan Terhadap Hasil Pemeriksaan Leukosit Urine Pada Pasien Infeksi Saluran Kemih (ISK) Krisdianti, Rika; Puspitasari, Puspitasari; Aliviameita, Andika
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1660

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Urinary Tract Infection (UTI) is a condition where there are microorganisms that multiply in the urinary tract causing inflammation and bacteriuria. The delay in the examination results in an error in diagnosis, the examination must be carried out >1 hour and a maximum of 2 hours after the voiding process at a temperature of 20-25℃ and the delay can be done a maximum of 4 hours at a temperature of 2-8℃. The study was conducted in June 2022. The study was conducted at SMK Muhammadiyah 1 Pandaan. UTI sampling at Sahabat Hospital in Suwayuwo Sukorejo. The purpose of this study was to determine the effect of delay time and storage temperature on the results of urine leukocyte examination in UTI patients. This study used an experimental method using 35 urine samples. The statistical test used the Two Way Anova test with a 95% confidence level and the inaccuracy limit of (α) = 0.05. Based on the results of the Two Way Anova test between storage temperature and delay in urine leukocytes, a significance value of 0.981 (p>0.05), it can be concluded that Ha is rejected and Ho is accepted, meaning that there is no interaction effect between the length of time delay and storage temperature on leukocyte results. urine in UTI patients.
The Effect Of Centrifugation Speed On The Results Of Urine Sediment Examination: Hubungan Pengaruh Kecepatan Sentrifugasi Terhadap Hasil Pemeriksaan Sedimen Urine Hasanah, Muslimah Uswatun; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 2 (2022): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i2.1668

Abstract

The process of centrifugation in urine examination is very important because it can be used in the manufacture of urine sediment. The purpose of this study was to determine the effect of centrifugation speed on the results of urine sediment examination. Centrifugation speeds used were 3000 rpm, 3500 rpm, 4000 rpm, and 4500 rpm using pathological urine samples. The samples used were 32. The research method used was an experimental laboratory at the Pusura Yos Sudarso clinical laboratory in Surabaya. The results of the study with a speed of 3000 rpm obtained an average of erythrocytes = 6.63/LPB, leukocytes = 13.63/LPB, and epithelial = 10.50/LPB. At a speed of 3500 rpm the average value of erythrocytes = 7.63/LPB, leukocytes = 15.13/LPB, and epithelium 11.63/PLB. At a speed of 4000 rpm the average value of erythrocytes = 8.63/LPB, leukocytes = 17.75/LPB and epithelial = 13.50/LPB. At a speed of 4500 rpm the average value of erythrocytes = 9.63/LPB, leukocytes = 20.50/LPB, and epithelial = 15.38/LPB. The results of the statistical test One Way Anova obtained p-value results of 0.001 < (0.05) in erythrocyte cells, p-value results for leukocytes 0.025 < (0.05), and p-value results for epithelial cells 0.002 < (0.05) means that there is an effect on the centrifugation speed on the results of urine sediment examination.
Perbedaan Hasil Pemeriksaan Hemoglobin Antara Metode Point of Care Testing Dengan Metode Sianmethemoglobin Pada Ibu Hamil Puspitasari, Puspitasari; Aliviameita, Andika; Rinata, Evi; Yasmin, Rizka Afifah Yonika; Saidah, Sukma Nur
Jurnal Analis Kesehatan Vol. 9 No. 1 (2020): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.73 KB) | DOI: 10.26630/jak.v9i1.2113

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Pemeriksaan kadar hemoglobin pada ibu hamil merupakan faktor yang penting karena sering ditemukan kondisi kurangnya zat besi selama masa kehamilan. Berbagai metode pemeriksaan hemoglobin digunakan pada fasilitas pelayanan kesehatan. Penelitian ini bertujuan untuk mengetahui perbedaan hasil pemeriksaan hemoglobin antara metode Point of Care Testing (POCT) menggunakan darah kapiler dan darah vena dengan metode sianmethemoglobin menggunakan darah vena pada ibu hamil. Penelitian ini merupakan penelitian eksperimental laboratorium dengan desain studi observasi analitik cross sectional. Penelitian dilakukan di Laboratorium Puskesmas Sukodono Kabupaten Sidoarjo pada Maret - April 2020. Sampel yang digunakan yaitu pasien ibu hamil di Puskesmas Sukodono berumur 15-34 tahun sebanyak 30. Hasil analisis menggunakan uji t tes sampel bebas didapatkan nilai t hitung sebesar 1,543 dengan p- value sebesar 0,128. Hasil analisis menggunakan uji t tes berpasangan didapatkan nilai t hitung sebesar 2,736 dengan p-value sebesar 0,011. Dapat disimpulkan bahwa tidak terdapat perbedaan yang signifikan secara statistik antara rata-rata hasil pemeriksaan hemoglobin metode POCT darah kapiler dengan metode sianmethemoglobin darah vena dan terdapat perbedaan yang signifikan secara statistik antara rata-rata hasil pemeriksaan hemoglobin metode POCT darah vena dengan metode sianmethemoglobin darah vena.
Manajemen Ekstrakurikuler Pramuka SD Negeri 02 Beluk Kecamatan Belik Kabupaten Pemalang Puspitasari, Puspitasari; Murniati, Ngurah Ayu Nyoman; Wuryandini, Endang
Journal Prakarsa Paedagogia Vol 5, No 2 (2022): Desember 2022
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24176/jpp.v5i2.8010

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Penelitian ini bertujuan untuk menganalisis dan mendeskripsikan manajemen ekstrakurikuler pramuka SD Negeri 02 Beluk Kecamatan Belik Kabupaten Pemalang. Jenis penelitian kualitatif fenomenologi.  Subyek penelitian adalah kepala Sekolah, guru, komite, wali murid, dan siswa.  Obyek penelitian adalah manajemen ekstrakurikuler pramuka meliputi perencanaan, pengorganisasian,  pelaksanaan, dan pengawasan. Teknik pengumpulan data menggunakan observasi, wawancara, dan dokumentasi. Analisis data menggunakan model Miles dan Huberman sedangkan untuk Uji Keabsahan menggunakan triangulasi. Hasil penelitian manajemen ekstrakurikuler mencakup: 1) Perencanaan  ektrakurikuler pramuka merupakan proses penyusunan dan pembentukan program kerja mulai dari uraian kegiatan, target, sasaran, waktu, penanggung jawab hingga sumber dana. 2) Pengorganisasian ekstrakurikuler pramuka merupakan proses pembagian kerja, sistem kerjasama antar personel, dan pembentukan struktur kelembagaan organisasi pramuka, jadwal dan materi pelatihan, pengelolaan sarana dan prasarana pendukung ekstrakurikuler pramuka. 3) Pelaksanaan ekstrakurikuler dilakukan baik secara administratif maupun fisik, dilaksanakan secara tatap muka sedangkan masa pandemik dilaksanakan secara virtual. Struktur materi pelatihan disusun dan disesuaikan tingkat syarat kecakapan umum pramuka siaga. 4) Mekanisme pengawasan dilaksanakan secara internal dan eksternal, bentuk pengawasan dilakukan melalui kunjungan secara langsung dan permintaan laporan kegiatan. Manajemen esktrakurikuler pramuka SD Negeri 02 Beluk berlangsung dengan baik dibuktikan dengan banyaknya prestasi yang diperoleh dan pelaksanaan fungsi manajemen yang efektif dan efisien
Co-Authors Abadi , Totok Wahyu Adhitya Marendra Kiloes, Adhitya Marendra Agustanto, Pulung Akbar, Aulia Rizky Fadhillah al-akbar, Wildan Amalia , Fara Putri Ambarani, Putri Yuli Ambarwati, Marfu'ah Amilya, Widya Intan Andika Aliviameita Anggi Anggraeni Kusumoningtyas Anita Anita Aprilianingsih, Sri Athi, Fina Umu Rif Bagus Kukuh Udiarto Baron, Pangeran Batis, Afifah Chylen Setiyo Rini Dewi Yanti Dindadianti , Afif Ida Endang Wuryandini, Endang Evi Rinata Haniah, Lutfi Atul Haryati Haryati Hasanah, Muslimah Uswatun Humesi, Lusi Ibnu Hamad Ilmi, Isyfa'ul Imamah, Siti Aliyatul Innabila, Syifa Intantri, Risma Iswari, Miranti Florencia Jamilatur Rohmah Karim, Erna Khomaroh, Annisatul Krisdianti, Rika Kuncoro, Sonni Kusumoningtyas, Anggi Anggraeni Maha, Maha Mardi Santoso Marwan Riki Ginanjar Masúd, Fajriyati Mas’ud B, Mas’ud B Maula, Nala Innayatul Miftahul Mushlih MUFIDAH, LAILATUL Muhammad Syafei, Muhammad Mukhamad Najib Muttaqijn, M. Imam Nasiro, Siti Ngurah Ayu Nyoman Murniati Nirosa, Givari Dwi Nuratiqah, Nuratiqah Nurfadhillah, Septy Primastuti, Yuridistya Putri Purwanti, Karunia Nurullita Putra, Ariza Bima Putri, Alifah Ananda R. Sapto Hendri Boedi Soesatyo Rahman, Rahmadani Ratna Wulandari Restiani, Restiani Rini, Chylen Setyo Riyandi, Ekwan Dwi Rofi’i, Aziz Rohmanudin, Rohmanudin Romdhoni , Arif Runturambi, Arthur Josias Saidah, Sukma Nur Saputra , Muhammad Rizky Sariyadi, Sariyadi Savitra, Igfina El Selviana Selviana Setiyo Rini, Chylen Silfia Silfia, Silfia Silitonga, Bentonius Situmeang, Boima Sriwijayanti, Sriwijayanti Stanislaus Riyanta Sunarmi Sunarmi Suzanna Ndraha Syah, Muhamad Jawal Anwarudin Syauqillah, Muhammad Syugiarto Syugiarto Tika, Setia Usman, Munjimayansari H. Verdiana, Verdiana Yasa, Ecep Suwardani Yasmin, Rizka Afifah Yonika Yeninastiti, Reny Zamzam Nurhuda