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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Penelitian Hutan Tanaman JURNAL PERBENIHAN TANAMAN HUTAN Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Perspektif : Review Penelitian Tanaman Industri E-Journal Widya Kesehatan dan Lingkungan JURNAL AGROTEKNOLOGI Jurnal Agro Journal of Tropical Biodiversity and Biotechnology Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Sains Natural: Journal of Biology and Chemistry Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jerami Jurnal Penelitian Tanaman Industri (Littri) Perspektif, Review Penelitian Tanaman Industri Jurnal Pusat Inovasi Masyarakat
Mohamad Rahmad Suhartanto
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jl. Meranti Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences

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Cryopreservation of Terap (Artocarpus elasticus Reinw. ex. Blume) Seeds: Viability and Structural Characterization Wardani, Fitri Fatma; Efendi, Darda; Suhartanto, Mohamad Rahmad; Purwoko, Bambang Sapta; Latifah, Dian
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.199-211

Abstract

Terap (Artocarpus elasticus Reinw. ex Blume) is an underutilized fruit tree native to Indonesia, valued both for its edible fruit and its diverse applications in traditional medicine. Terap seeds are recalcitrant, so cryopreservation is the most effective method for their long-term preservation. This study aimed to determine the optimal loading and vitrification times for cryopreservation to maintain seed viability. A factorial, completely randomized design was employed with two factors: loading time (0 and 20 min) and vitrification time (0, 30, and 60 min). Seed viability after loading and vitrification treatment with or without freezing in liquid nitrogen was evaluated using tetrazolium chloride (TTZ) and germination tests. Results indicated that the loading and vitrification treatments did not yet improve the viability of terap seeds after cryopreservation, as determined by the TTZ and germination tests. The TTZ test showed that the viability of all treated seeds decreased, regardless of whether they were frozen in liquid nitrogen. Germination tests revealed that all treated seeds without freezing had reduced germination, while none of the seeds treated with freezing germinated. Structural analysis indicated that freezing alters cell degradation, likely due to the formation of ice crystals and mechanical stress. To improve the success of terap seed cryopreservation, future efforts should focus on optimizing exposure times, reducing PVS2 toxicity, and enhancing cryoprotectant penetration by removing the seed coat or using the embryo axis as an explant.
Co-Authors ABDUL MUNIF Abdul Munif Abdul Qadir Agus Purwantara AGUS PURWANTARA Agus Purwantara Agus Ruhnayat AHMAD JUNAEDI Ahmad Zamzami Akhiruddin Maddu Alfarabi, Emir Aqsha Ani Kurniawati Ari Wahyuni Arodi Agustenta Sinulingga Astryani Rosyad Aulia Hasan Widjaya Baharudin Baharudin ; BAHARUDIN BAHARUDIN Bambang Sapta Purwoko Cahyani, Gesa Nur Cintaning, Anis Bias Daniel Happy Putra Dede J. Sudrajat Dede J. Sudrajat DEVI RUSMIN Diaguna, Ridwan Dian Latifah DIAN LATIFAH, DIAN Dirgahani Putri Dyah - Manohara Edi Santosa Efendi, Darda Eka Maulidiya, Sherly Endah R. Palupi Endah Retno Palupi Endang Gati Lestari ENDANG MURNIATI Eny Widajati Eprilian, Husna Fatima Evayusvita Rustam Evayusvita Rustam Fadillah, Arvin Muhammad Fatiani Manik Fatiani Manik Firdaus, Jonni Fitri Fatma Wardani Fitri Viva Yuningsih, Aida Fitriansyah, Muhammad Ramdhani Heri Ahmadi Heru Setiawan Heru Setiawan Ikasari, Yulfa Astuti Karmaita, Yummama Kartika Kartika Kartika Kartika Ketty Suketi Khairani Khairani Lainufar, Putri Aulia Lilih Naelun Najah Luluk Prihastuti Ekowahyuni Manohara, Dyah - Maryati Sari Mubarak, Farahdina Muhamad Noor Azizu, Muhamad Noor Muhamad Syukur Muhammad Abdul Rahman Hakim Muhammad Rofiq Nabila, Diah Ayu Nafisah Nafisah Neti, Natali NI KADEK EMA SUSTIA DEWI Nurfiana, Yuni NURUL HIDAYAH Nurul Hidayah Nurul Rostami Dewi Nurul Rostami Dewi Otih Rostiana Palupi, Endah R. Pipin Apriani Pradana, Farhan Novfourthino Purwono Purwono, Purwono Putri Mian Hairani Putri, Lystiana Dewi Qadir, Abdul Risa Wentasari Roedhy Poerwanto Rokhani Hasbullah Rukundo, Jean D’amour RUSMIN, DEVI Satriyas Ilyas SATRIYAS ILYAS Setyowati, Nur Farida Sinaga, Tamara Rudang Astari Sintho Wahyuning Ardie SRIANI SUJIPRIHATI Suci Rahayu Supramana Surjono Hadi Sutjahjo Suryo Wiyono Suwarto Suwarto Syarifa Mustika Syarifa Mustika Taisa, Rianida Tatiek K. Suharsi Tatiek K. Suharsi Tatiek Kartika Suharsi Usman Ahmad Widodo Widodo Zulfa Fauziyyah Taini