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The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures Diba, Lucky; Suzanita Utama; Tjuk Imam Restiadi; Suherni Susilowati; Nusdianto Triakoso; Dwi Wijayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.81-89

Abstract

The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was collected through massage in the morning. The pooled semen sample was divided into four treatment groups. In the T0 group the semen was diluted in 0.9% NaCl, while in the T1, T2 and T3 groups the semen was diluted in 0.9% NaCl added with 5, 10 and 15% egg yolk. The results showed that the motility and viability of spermatozoa decreased when stored at cool temperatures for ten hours (p <0.05). Semen of roosters stored at 5°C in saline solution without the addition of egg yolks showed the lowest motility and viability of spermatozoa (p <0.05). The addition of egg yolk into the saline extender increased the motility and viability of spermatozoa. Concentration of 15% egg yolk in saline solution resulted in the highest spermatozoa motility and viability when stored for up to 8 hours (p <0.05). However, motility and viability of spermatozoa at 10 hours of storage were not significantly different (p >0.05) with the addition of 10% and 15% egg yolks. Therefore, it could be concluded that the addition of 15% egg yolk into a saline solution as an extender could maintain the motility and viability of kampung rooster spermatozoa when stored at 5°C for 10 hours.
Reproduction record of captive Sumatera elephant (Elephas maximus sumatranus) at Way Kambas National Park, Indonesia Sofie Adi, Keren Jayanti; Budiarto Budiarto; Hermin Ratnani; Pudji Srianto; Budi Utomo; Suzanita Utama; Setiawan Koesdarto; Nurhusien Yimer
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.157-165

Abstract

This research aims to determine reproductive data on Sumatran elephants (Elephas maximus sumatranus) in the ETC and ERU of Way Kambas National Park, Indonesia during 1988-2021. Data recorded from the elephant population at both locations (ETC and ERU) includes the number, gender of elephants, elephant calves, ages and birth dates. Calving intervals and service periods were calculated from calving records of cows with a minimum parity of two. Data collection produced data on 47 elephant calves from 13 female elephants with at least twice the parity and 12 primiparous cows. Elephant reproductive records at ETC and ERU were: age (37.44 ± 9.03 vs. 29.75 ± 3.30 years), parity (2.78 ± 1.09 vs. 2.50 ± 0.58), and age at first birth (18, 11 ± 3.92 vs. 17.75 ± 0.50 years). While the calving interval was 1857.56 ± 870.81 vs. 1833.00 ± 305.18 days, and service period respectively 1229.44 ± 846.18 vs. 1210.50 ± 283.59 days, respectively. It can be concluded that the calving interval and service period for captive elephants at ETC and ERU were not much different and were within the normal range. Young cows showed better reproductive efficiency than older cows. Monitoring calves is very important. Further assistance is needed to improve elephant mobile veterinary services, increase diagnostic laboratory capacity, and educate camp managers, veterinary assistants, and mahouts about elephant diseases, their monitoring and treatment. In addition, the use of reproductive technology such as monitoring ovulation using ultrasound and carrying out artificial insemination was expected to increase reproductive efficiency.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis.
Efektivitas krim ekstrak daun melinjo (Gnetum gnemon L.) terhadap jumlah sel fibroblas pada luka bakar derajat II dalam tikus putih (Rattus norvegicus) Sulaiman, Azhari Athaillah; Maslachah, Lilik; Utama, Suzanita; Yuniarti, Wiwik Misaco; Sukmanadi, Moh.; Hidajati, Nove
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.693

Abstract

Burn wound is an injury that causes tissue damage, typically due to contact with a heat source. Fibroblasts have an important role in wound healing, synthesizing collagen as the main constituent of the extracellular matrix which is important in wound integrity. The purpose of this study was to determine the effectiveness of cream of melinjo (Gnetum gnemon L.)  leaf extract on the number of fibroblast cells in deep partial thickness burn in white rats (Rattus novegicus). Twenty male white rats (Rattus norvegicus) were divided into five treatment groups, with four replications in each group, and were induced with in deep partial thickness burn. The negative control group (K-) was treated with cream base, the positive control group (K+) was treated with Silver Sulfadiazine, while P1, P2, and P3 group was treated with respectively 2.5, 5 and 10% melinjo (Gnetum gnemon L.) leaf extract cream. Therapy was given twice a day for 14 days. Oneway ANOVA showed significant differences between each treatment group (p <0.05) and continued with the Duncan test. The average number of fibroblasts in each treatment group showed that the K- were significantly different from the K+, P2, P3 groups but not P1. The lowest mean number of fibroblast cells was found in the K- group. The conclusion from the research that has been carried out was that melinjo (Gnetum gnemon L) leaf extract cream decrease the number of fibroblast cells in second-degree burns wounds in white rats (Rattus norvegicus).
Peningkatan nilai produk olahan hasil laut dan gerakan gemar makan ikan bagi kelompok istri dan anak nelayan guna mencegah stunting [Increasing the value of processed sea products and the fish-eating movement for groups of fisherman wives and children to prevent stunting] Rachmawati, Kadek; Mulyati, Sri; Utama, Suzanita; Wurlina, W; Santoso, Kuncoro P.; Suwasanti, Ni Luh
Buletin Pengabdian Vol 4, No 1 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i1.35366

Abstract

This Community Service Activity is carried out to educate fishermen's wives on how to process marine products such as fish into processed foods that children like so that children will be more fond of eating fish, processed innovations using raw materials from marine products into dry products and wet products with high-quality nutritional value with high selling value, and increasing the love of eating fish among fishing families to increase intelligence and prevent stunting. The methods used are education, training, and mentoring for innovations in processed seafood which are popular with children and have high selling value, apart from that, education is also carried out about stunting and how to prevent stunting. The result of this PKM activity is that fishermen's wives can make various foods and snacks made from marine products that are popular with children and have value for sale so that they can provide income for fishermen's wives, as well as increase the understanding of fishermen's wives about stunting to prevent the incidence of stunting in fishermen's children and increase the movement for eating fish among fishermen's families.
Keberlanjutan UMKM olahan hasil laut di Pesisir Bulak Surabaya guna meningkatkan daya saing di era new normal (Sustainability of MSMEs processed marine products in Bulak Coastal Surabaya to increase competitiveness in the new normal era) Wurlina, W; Racmawati, Kadek; Utama, Suzanita; Mahasri, Gunanti; Mulyati, Sri; Suwasanti, Niluh
Buletin Pengabdian Vol 2, No 2 (2022): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v2i2.25217

Abstract

The Regional Leading Product Development Program (RLPDP) activities are carried out to sustain the existence of Micro, Small, and Medium Enterprises (MSMEs) processed marine products in increasing marketing in the new normal of the Covid-19 Pandemic period. This makes the implementation team assist the strategies used by MSMEs and the effectiveness of these strategies. In this activity, the factors influencing MSMEs sustainability were product innovation, packaging, and marketing. The method used was education, training, and mentoring for the innovation of processed seafood using packaging according to its function and marketing. The result of RLPDP was MSMEs have implemented innovations in processed marine products with packaging accompanied by the length of use, labeling, business permits, and halal accompanied by slogans. Marketing of seafood products processing was carried out through the Surabaya UKM center, namely the Siola MSMEs Center, the SIB (Bulak Fish Center) MSMEs, Merr MSMEs, hotels, and participation in exhibition activities organized by the Surabaya City Government and East Java Province. Furthermore, to expand marketing reach, socialization has been carried out through social media such as WhatsApp, Facebook, Instagram, and e-mail, as well as the marketplaces such as Tokopedia, Bukalapak, and Blibli.
Pemulihan dan pengembangan ekonomi kreatif olahan hasil laut di Pesisir Bulak Surabaya pada era new normal untuk menunjang pertumbuhan ekonomi (Recovery and development of creative economy processed sea products in coastal Bulak Surabaya in new normal era for supporting economic growth) Wurlina, W; Racmawati, Kadek; Utama, Suzanita; Mahasri, Gunanti; Suwasanti, Niluh; Putri, Desak Ketut Sekar Cempaka
Buletin Pengabdian Vol 1, No 1 (2021): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v1i1.20241

Abstract

The Regional Prominence Product Development Program (RPPDP) aims to determine the impact of thecoronavirus disease2019(COVID-19)pandemic on Micro, Small, and Medium Enterprises (MSMEs) based on the creative economy of seafood culinary in Bulak, Surabaya. This program was conducted to find out the solution that can be taken by government to recover and develop the creative economy of seafood culinary entrepreneur due to the COVID-19 pandemic impacts. Data were collected by interview/questionnaire, literature review, and implementation of recovery of the the creative economy of seafood culinary. The period of this study was from March (the beginning of the COVID-19 pandemic in Indonesia) to the middle of December 2020. The results of RPPDP showed that there were two elements influenced the seafood culinary entrepreneur due to the COVID-19 pandemic, namely Human Resources and innovation processing and marketing products. The government can take steps to overcome these problems, such as providing business capital assistance, giving discounts on electricity bills, deferring loan payments, assisting MSMEs who closed their businesses as result of the pandemic to transform or start new businesses. In conclusion, innovation of seafood culinary during the pandemic should be in accordance with the market demand, innovating traditional souvenirs to be work from home snacks, and online marketing using non-cash payment.
Co-Authors . Helen . Mulyani . Purwati Abdul Samik, Abdul Abdullah Hasib Adikara, Yuanara Augusta Rahmat Akbar Haryo Widhi Trismiharto Al arif, Mohammad Anam Alfanindya, Evita Aisyah Amrullah, Muhammad Fajar Belgania, Ristaqul Husna Berliana, Erprinanda Galuh BUDI UTOMO Budi Utomo Budiarto Budiarto Candra Bumi Chairul Anwar Chandra, Evania Haris Chandramohan, Koshini Desak Ketut Sekar Cempaka Putri Dhesy Kartikasari Diba, Lucky Djoko Legowo Djoko Poetranto DWI WIJAYANTI Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Erma Safitri ERMA SAFITRI Fedik Abdul Rantam Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Ida Yuniarti IMAM MUSTOFA Imam Mustofa Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Kadek Racmawati Kartikasari, Dhesy Kevin Mardotillah Khairullah, Aswin Rafif Koesnoto Supranianondo Kuncorojakti, Suryo Laba Mahaputra Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mas’ud Hariadi Mayola, Dilla Aranda Meles, Dewa Ketut Mochamad Lazuardi Muhammad Fahmi Abdillah Muhammad Faizal Adiutomo Ngakan Made Rai Widjaja Niluh Suwasanti Nunuk Dyah Retno Lastuti Nurhusien Yimer Nusdianto Triakoso Pratama, Hanif Sabekti Primarizky, Hardany Pudji Srianto Putri, Desak Ketut Sekar Cempaka R. Heru Prasetyo R.T.S Adikara, R.T.S Adikara Racmawati, Kadek Ratna Damayanti Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Santoso, Kuncoro P. Sarudji, Suryanie Setiawan Koesdarto Sharveihn Sathindren Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Wigati Mardi Suherni Susilowati Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Suwasanti, Ni Luh Suwasanti, Niluh Syaiful Rizal Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Widjiati w Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wurlina, W Xin, Irene Teh Kai Yunus, Muchammad