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All Journal Jurnal Entropi Sainstek Microbiology Indonesia JURNAL ILMU PERTANIAN Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL PENDIDIKAN GLASSER Jurnal Biologi Tropis Jambura Edu Biosfer Journal Jambura Journal of Chemistry Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Ilmiah Wahana Pendidikan BIOVALENTIA: Biological Research Journal Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Pendidikan, Sains dan Teknologi Prosiding Seminar Nasional Mini Riset Mahasiswa Jurnal Pengabdian Masyarakat Teknologi Pertanian Journal of Tropical Mycorrhiza Biodik: Jurnal Ilmiah Pendidikan Biologi HUMANIORASAINS Damhil: Jurnal Pengabdian kepada Masyarakat Inventa: Journal of Science, Technology, and Innovation Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Biodiversitas Journal of Biological Diversity Nusantara Bioscience
Yuliana Retnowati
Universitas Negeri Gorontalo
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Antagonisme Trichoderma sp. terhadap Colletotrichum capsici: Uji In Vitro sebagai Strategi Pengendalian Hayati Penyakit Antraknosa pada Cabai Rawit Triska Wahyuni Dawit; Ani M. Hasan; Jusna Ahmad; Yuliana Retnowati; Febriyanti Febriyanti
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/dvr6m616

Abstract

Anthracnose disease is a plant disease caused by the fungus Colletotrichum capsici, the disease is one of the problems in the cultivation of cayenne pepper (Capsicum frutescens). This study aims to test the antagonistic ability of Trichoderma sp. against the fungus Colletotrichum capsici and observe the morphological changes in Colletotrichum capsici colonies exposed to Trichoderma sp in vitro on cayenne pepper plants Capsicum frutescens. The test method was carried out using the dual culture method to determine the percentage of inhibition by studying the interaction between Trichoderma sp. and Colletotrichum capsici colonies on Potato Dextrose Agar culture media. The results showed that Trichoderma sp. fungi can compete for space and resources against Colletotrichum capsici fungi macroscopically by controlling space and resources, and microscopically by producing antibiotics (antimicrobials) that can inhibit the growth of Colletotrichum capsici. The urgency of this research is the need for safe and environmentally friendly disease control efforts. One method that has been widely developed is by utilizing biological agents, such as Trichoderma sp. which is able to inhibit the growth of the pathogen Colletotrichum capsici.
Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia ABUBAKAR SIDIK KATILI; YULIANA RETNOWATI
Biodiversitas Journal of Biological Diversity Vol. 18 No. 2 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180256

Abstract

Katili AS, Retnowati Y. 2017. Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 826-833. Actinomycetes is a group of positive gram microorganism known to produce secondary metabolic compounds that work as antibiotics, anti-fungus, anti-virus, anti-cancer, and other important enzymes for the industry. This research aimed at discovering the potentials of Actinomycetes of the mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. It focused on finding out the diversity of Actinomycetes at mangrove ecosystem in Torosiaje. Hence, various types of Actinomycetes that have potentials to create secondary metabolic compounds/bioactive compounds for industrial purposes can be found. This purpose can only be attained through isolation of actinomycetes. This isolation was conducted through characterization of morphological characteristics of the colony and the spore, molecular characterization through isolation of DNA genome of the actinomycetes, amplification of 16S rRNA through PCR, sequencing, and reconstruction of the phylogenetic tree. We were able to obtain the actinomycetes isolate from the sediment in rhizosphere area of Ceriops tagal, Bruguiera gymnorrhiza, Xylocarpus sp, Rhizophora apiculata, Avicennia sp, and Sonneratia alba species of mangrove. Each isolated actinomycetes has specific morphological characteristics. The density of isolated actinomycetes in Torosiaje mangrove area was very low with an average of 1 x 105 CFU gram-1 in each sediment.
Diversity of antibiotic-producing Actinomycetes in mangrove forest of Torosiaje, Gorontalo, Indonesia YULIANA RETNOWATI; LANGKAH SEMBIRING; SUKARTI MOELJOPAWIRO; TJUT SUGANDAWATY DJOHAN; ENDANG SUTARININGSIH SOETARTO
Biodiversitas Journal of Biological Diversity Vol. 18 No. 4 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180421

Abstract

Retnowati Y, Sembiring L, Moeljopawiro S, Djohan TS, Soetarto ES. 2017. Diversity of antibiotic-producing actinomycetes in mangrove forest of Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 1453-1461. Actinomycetes for antibiotic production have been studied at various extreme environments. Mangrove forest of Torosiaje in Gorontalo Province, Indonesia has unique geomorphological conditions where the forest is surrounded by karst ecosystem consisting of fringe and overwash types. Therefore, the objective of this study was to analyze the distribution and diversity of antibiotic-producing Actinomycetes in various rhizosphere including different locations and mangrove species. Samples of rhizosphere soil were collected in the depth of 0-10 cm, which was then subjected to detailed physicochemical analysis. Actinomycetes were collected through heating pre-treatment (60oC for 15 min) followed by culturing in the Starch Casein Agar medium supplemented with cycloheximide and nystatin. The screening process of antibiotic-producing Actinomycetes was based on Agar block method against pathogenic microorganisms. Grouping of Actinomycetes was determined by ARDRA fingerprinting analysis. The diversity of Actinomycetes was analyzed based on sequencing of 16S rDNA. The results showed that the distribution of Actinomycetes was found in overwash type, middle zone and upper zone of fringe type including rhizosphere of 7 species of mangrove. The highest population of Actinomycetes was found in rhizosphere of R. mucronata at the overwash type, and the lowest one found in rhizosphere of R. apiculata at the middle zone of fringe type. A total of 77 isolates amongst 167 isolate collection showed antibacterial activities. Forty seven representatives from 77 antibacterial-activities isolates were selected using ARDRA for partial characterization according to their phylogenetic diversity. Sequencing and analysis of 16S rDNA from selectedrepresentative isolates displayed the presence of members associated with Actinomycetes genera such as Streptomyces, Amycolatopsis, Saccharomonospora, and Nocardiopsis. The member of genus Streptomyces such as Streptomyces qinglanensis and Streptomyces champavatii were distributed across locations. Genus Saccharomonospora and Nocardiopsis were mostly found at the overwash type, while Amycolatopsis was found at the upper zone of fringe type.
Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia YULIANA RETNOWATI; SUKARTI MOELJOPAWIRO; TJUT SUGANDAWATY DJOHAN; ENDANG SUTARININGSIH SOETARTO
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190627

Abstract

Retnowati Y, Moeljopawiro S, Djohan TjS, Soetarto ES. 2018. Antimicrobial activities of actinomycetes isolates from rhizospheric soil on different mangrove forests of Torosiaje, Gorontalo, Indonesia. Biodiversitas 19: 2196-2203. Mangrove forests are very productive ecosystems that form unique saline environment very rich in organic matter, containing nitrogen and sulfur available for microorganisms. Mangrove forest as an extreme environment is promising to be sources of antibiotic-producing actinomycetes. The objectives of this study were to analyze the antimicrobial activities of metabolites produced by actinomycete isolates from rhizospheric soil of mangrove forest of Torosiaje, Gorontalo, Indonesia, and identify the active compound for novel antibiotics production. Six isolates from a coastal mangrove forest was selected to produce secondary metabolite. The crude extract of the six selected actinomycete isolates showed antimicrobial activities against pathogenic microbes; the highest antimicrobial activities was indicated by metabolite produced by FUAm2-h1 and FMBg2-x3 isolates. The metabolite crude extracts produced by two potential isolates inhibited growth of pathogenic microbe on MIC value of 0.0625 to 0.5mgmL-1. Bio-autography assay detected an active compound on Rf value of 0.94, especially on extracellular metabolite produced by strain FUAm2-h1. The bioactive compounds were identified by liquid chromatography joined with low-resolution mass spectroscopy (LC/MS) and analysed by MEDINA's database The active compounds composed of 17 substances, and only 3 substances showed a high quantity with molecular weight of 507.37, 344.32 and 563.66 mol G-1, respectively. FTIR analyses identified the functional groups in the active compounds consisted of amide, amine, alkuna, alkane, NO2 nitro compound, alcohol, ether, carboxylic acid, ester and C-H aromatic ring. The biosynthesis of antibiotic on FUAm2-h1 and FMBg2-x3 isolates was regulated by double genes, i.e., PKS-II and NRPS genes. The antimicrobial activities of two actinomycete isolates showed the performance of antibiotics suspected as aromatics polyketides group. The FUAm2-h1 and FMBg2-x3 isolates showed high similarity with Streptomyces qinglanensis strain 172205 and Streptomyces sanyensis strain 219820, respectively, in terms of 16S rRNA gene sequences. The potential of those selected actinomycetes from extreme environments of mangrove forest constitute a source of promising actinomycete strains producing biologically active secondary metabolites.
Identification of fermentative bacteria on local microorganisms of golden snail (Pomacea canaliculata Lamarck, 1822) YULIANA RETNOWATI; Abubakar Sidik Katili
Biodiversitas Journal of Biological Diversity Vol. 22 No. 2 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220231

Abstract

Abstract. Retnowati Y, Katili AS. 2021. Identification of fermentative bacteria on local microorganisms of golden snail (Pomacea canaliculate Lamarck, 1822). Biodiversitas 22: 778-784. Local Microorganisms (LMo) is a fermented liquid containing various microorganisms that potentially act as decomposers and bio-fertilizer. P. canaliculata is one of the rice pests that is a basic ingredient of LMo because of its high protein content. The objective of this study was to determine the types of fermentative bacteria on Local Microorganisms of P. canaliculata. The fermentation of LMo was conducted for 0, 7, 14, and 21 days. Microbial population was determined at 7-day intervals based on the TPC method. Characterization and identification based on polyphasic taxonomy including macroscopic and microscopic morphological characters., Molecular identification was based on 16S rRNA gene sequences. The results showed that LMo of P. canaliculata had a low degree of acidity and tended to decrease during the incubation period, from pH 5.3 to 4.0. Bacterial population tends to increase at 0-14 fermentation days and decreases after 21 days. The isolation results showed that the 3 bacterial isolates namely BFPc-01, BFPc-02, and BFPc-03 were isolated based on morphological differences. The morphological characters of BFPc-01 was milky white color colony, Gram-negative, coccus; BFPc-02 isolate was pink, colony color, Gram-negative, coccus; and BFPc-03 isolate was yellow color colony, bacillus, Gram-positive. The results of molecular characterization based on the 16s rRNA gene sequence showed that BFPc-01 isolate similar to Klebsiella pneumoniae MT604895.1 (99.04%), BFPc-02 isolate closely related to Serratia sp. (100%), and BFPc-03 isolate similar to Microbacterium  sp. (100%).
Antibacterial activity of sponge-associated bacteria from Torosiaje marine area, Gorontalo, Indonesia YULIANA RETNOWATI; ABUBAKAR SIDIK KATILI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240255

Abstract

Abstract. Retnowati Y, Katili AS. 2023. Antibacterial activity of sponge-associated bacteria from Torosiaje marine area, Gorontalo, Indonesia. Biodiversitas 24: 1151-1156. The marine sponge is a member of the Porifera class animal group that has the potential to produce secondary metabolites with various biological activities. These marine animals are often associated with various bacteria from the actinomycetes and non-actinomycetes groups. Currently, information about bacteria associated with sponges in Gorontalo coastal waters is still very limited. Bacteria associated with organisms from the marine environment have been explored as producers of new types of bioactive compounds. Exploring bacteria associated with sponges in the coastal waters of Gorontalo can potentially find new types of antibiotics. The aim of this study was to reveal the diversity of antibiotic-producing bacteria associated with sponges in the Torosiaje coastal area, Gorontalo. The research was carried out in the Torosiaje marine area as sponge sampling locations, including seawater physicochemical properties measurement. The sponges were identified based on morphological characteristics. The sponge-associated bacteria were isolated and screened for antibacterial potential against Escherichia coli and Staphylococcus aureus. The potential sponge-associated bacteria were identified based on a molecular approach. The result showed that a total of 10 sponge members of Demospongiae class. All ten sponge-associated bacteria were non-actinomycetes bacterial isolates that showed similar morphological characteristics. Only isolate ILM-1 associated with Coelocarteria singaporensis showed antibacterial potential in broad-spectrum mode against Escherichia coli and Staphylococcus aureus. The ILM-1 isolate was identified as Vibrio diabolicus closely related to Vibrio diabolicus strain CW-9-11-1 with a similarity index of 99.70%.
Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia YULIANA RETNOWATI; NOVRI YOULA KANDOWANGKO; ABUBAKAR SIDIK KATILI; WAWAN PEMBENGO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250301

Abstract

Abstract. Retnowati Y, Kandowangko NY, Katili AS, Pembengo W. 2024. Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo, Indonesia. Biodiversitas 25: 907-915. The objective of this study was to isolate and identify the actinomycetes associated with the rhizosphere of plants in the karst ecosystem. This study was descriptive-quantitative. Soil sampling was conducted at three locations of the karst ecosystem of Gorontalo, i.e., Bangga Hills at Bangga Bubaa Coastal area of Gorontalo District, Hills around Lake Limboto of Gorontalo District, and Oluhuta Beach Hills at Bone Bolango District. Rhizosphere-soil sample was collected from 15-30 cm of soil depth. Isolation of actinomycetes was performed using the plate method. The screening of phosphate-solubilizing bacteria was based on the ability to solubilize phosphates indicated by the clear zone on the Pikovskaya medium. Identification of actinomycetes bacteria based on morphology and molecular characters. The morphology character included the color of aerial and substrate mycelium. Molecular characteristics based on the sequence of 16S rRNA gene compared to sequence data on the gene bank of NCBI. The phylogenetic tree reconstruction was based on the Neighbor-joining algorithm. The results successfully found six isolates, including three actinomycetes isolates, two non-actinomycetes bacteria, and one yeast. They were isolated from the rhizosphere of Malastoma malabathrum, Chromolaena odorata, Cycas rumphii, Leucaena leucocephala, and Jatropha curcas. The actinomycetes isolate showed plant-growth-promoting potential indicated by solubilizing phosphate and Indole 3-Acetic Acid production activities. Based on the molecular analysis, actinomycetes were closely related to Streptomyces aegyptia (MT505707.1:42-1426), Streptomyces sp. GGCR-6 (MH718844.1:5-1391), and Streptomyces carpaticus strain PES-A23 (MH712039).
Molecular identification of rhizospheric Actinomycetes from karst ecosystems of Gorontalo, Indonesia, and its seed germination induction capability of Zea mays var. doti YULIANA RETNOWATI; ABUBAKAR SIDIK KATILI; NOVRI YOULA KANDOWANGKO; WAWAN PEMBENGO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 12 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251212

Abstract

Abstract. Retnowati Y, Katili AS, Kandowangko NY, Pembengo W. 2024. Molecular identification of rhizospheric Actinomycetes from karst ecosystems of Gorontalo, Indonesia, and its seed germination induction capability of Zea mays var. doti. Biodiversitas 25: 4763-4771. Karst, as an extreme ecosystem, was a source of diverse Actinomycetes with varied biological activities. This study explored the plant-growth-promoting potential of rhizospheric Actinomycetes from the karst ecosystem of Gorontalo, with a focus on inducing seed germination in Zea mays var. doti. Four locations in Gorontalo were selected to explore Actinomycetes, targeting approximately 20 different rhizospheric plant species to isolate these microorganisms. Among the 25 isolates obtained, representing diverse morphological types from 12 rhizospheric plants, eight actinomycete isolates exhibited phosphate-solubilizing activity and produced indole-3-acetic acid (IAA). The 16S rRNA gene sequence analysis showed that approximately 75% of the isolates belonged to the Streptomyces genus, including Streptomyces cavourensis strain KRZm-02, Streptomyces sp. strain KRZm-03, Streptomyces pratensis strain KRLl-01, Streptomyces carpaticus strain KRIt-01, Streptomyces sp. strain KRIt-02, and Streptomyces aquilus strain KRPa-01. Additionally, 12.5% of the isolates were identified as Nocardiopsis alba strain KRZm-01 and Micromonospora sp. strain KRPt-01, respectively. The two isolates with the highest plant-growth-promoting potential, Streptomyces pratensis strain KRLl-01 and Streptomyces carpaticus strain KRIt-01, were further tested for their ability to promote germination of Zea mays var. doti seeds over 7 days. Among the two, Streptomyces carpaticus strain KRIt-01 exhibited the highest germination-inducing potential. Overall, the karst ecosystem of Gorontalo offers a valuable reservoir of biological resources with the potential for Plant-Growth-Promoting Rhizobacteria (PGPR). Further studies on the application of these actinomycete isolates as biofertilizers in agricultural and plantation crops could significantly contribute to improving crop growth and productivity, thereby revolutionizing agricultural practices.
Bioprospecting and molecular identification of Streptomyces on karst ecosystems in the coastal area of Gorontalo, Indonesia, as plant growth-promoting rhizobacteria YULIANA RETNOWATI; ABUBAKAR SIDIK KATILI; NOVRI YOULA KANDOWANGKO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 7 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260721

Abstract

Abstract. Retnowati Y, Katili AS, Kandowangko NY. 2025. Bioprospecting and molecular identification of Streptomyces on karst ecosystems in the coastal area of Gorontalo, Indonesia, as plant growth-promoting rhizobacteria. Biodiversitas 26: 3294-3301. Agricultural systems that rely on synthetic pesticides, chemical fertilizers, herbicides, and monoculture farming systems contribute to declining soil fertility and affect the soil microbial community. In contrast, organic farming systems incorporate Plant Growth-Promoting Rhizobacteria (PGPR) as a potential solution. Therefore, this study aimed to explore the prospecting of Streptomyces from the rhizosphere of plants in the karst ecosystem PGPR on the coastal area of Gorontalo by using a molecular approach to identify promising strains. The success of this study in isolating and identifying three promising Streptomyces strains is a testament to the robustness of this study. Soil sampling was conducted at three locations at a 15-30 cm depth. The PGPR potential of Streptomyces was determined based on phosphate-solubilizing activity, Indole acetic acid production, antagonism to the root pathogenic fungus, and tolerance to fungicide capability. This study successfully isolated three Streptomyces from Jatropha curcas rhizosphere. In line with these findings, 16S rRNA sequencing showed the affinity of the strain to the genus Streptomyces on 98.39 to 99.65% of homology, including Streptomyces sp. strain KRJc-01, Streptomyces rochei strain KRJc-02, and Streptomyces pratensis strain KRJc-03. The initial in vitro assay showed the potential ability of Streptomyces sp. in phosphate-solubilizing activity, indole acetic acid production, antagonistic against root-pathogenic fungi, and fungicide tolerance capability. Antagonistic activity against the root-pathogenic fungus of Fusarium oxysporum is supported by PKSs and NRPS gene cluster. In general, these findings suggest that Streptomyces sp. from the rhizosphere of plants in the karst ecosystem within Gorontalo has significant potential as PGPR, contributing to sustainable crop improvement.
Detection of Vibrio sp. on vaname shrimp (Litopanaeus vannamei) cultivation pond in Gorontalo, Indonesia PENI ULFIANI; ANI M. HASAN; YULIANA RETNOWATI
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140216

Abstract

Abstract. Ulfiani P, Hasan AM, Retnowati Y. 2022. Detection of Vibrio sp. on vaname shrimp (Litopanaeus vannamei) cultivation pond in Gorontalo, Indonesia. Nusantara Bioscience 14: 340-346. Vibrio is a pathogenic bacteria that can attack cultured shrimp. Therefore, it is necessary to increase the intensity of water changes and efforts to control water quality to maintain dissolved oxygen levels and manage organic matter content in ponds to prevent the increase of pathogenic bacteria, especially Vibrio. In addition, water physicochemical factors can influence the presence and population of Vibrio bacteria in ponds. This study aims to determine the presence of Vibrio sp. in vaname shrimp culture ponds in Gorontalo, Indonesia. Descriptive quantitative analysis was used to analyze the data collected from this study. Shrimp pond water samples were obtained from shrimp ponds in Molosipat Village, West Popayato Sub-district, Pohuwato District, Gorontalo, Indonesia, using a purposive sampling technique with an interval of three days from the first water change to the last before the water change in one period (approximately 10 days). The water samples were subjected to physicochemical analysis, including temperature, pH, salinity, ammonia, nitrate, and dissolved oxygen. The results showed that there were Vibrio bacteria in the Thiosulfate Citrate BileSalt Sucrose Agar (TCBSA) medium with a population that tends to increase in the range of 1.0 × 103 - 2.7 × 104 CFU/mL. The isolation results showed that two isolates were named ISL1 and ISL2, with the colony morphology characters of ISL1 isolates being green and ISL2 isolates being yellow. The results of microscopic observations of the two isolates showed comma-shaped and gram-negative cells. Molecular identification using the 16S rRNA gene and reconstruction of the phylogenetic tree using the Neighbor-Joining algorithm showed that ISL1 isolate is very closely related to Vibrio parahaemolyticus strain 11-1 (relationship level 99%) and ISL2 is closely related to V. parahaemolyticus strain B2-1 (relationship level 86%).
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Ahmad, Aisyah Aisyah Ahmad Aisyah Ahmad Alfandi Kibu Angio, Melisnawati H. ANI M. HASAN Ani M. Hasan Ani M. Hasan Ani M. Hasan Ani M. Hasan Apriliyanti Mohulaingo Aryati Abdul Asep S. Abdurrahmat Asniawati Zainuddin Asniwati Zainuddin Budy Santosa Budy Santoso Chairunnisah J Lamangantjo Chairunnisah J Lamangantjo Citra Leonita Matalauni Cono, Titi Hawanda Metania Dama, Dewi Rahayu Danial Mohamad Devi Bunga Pagalla Dewi Farhamni Dewi Wahyuni K Baderan Dewi Wahyuni K. Baderan Djuna, Lamondo Eka Zahra Solikahan Eman Rahim ENDANG S. SOETARTO ENDANG SUTARININGSIH SOETARTO ENDANG SUTARININGSIH SOETARTO Fatma Novriyanti Abdul febriyanti febriyanti Febriyanti Febriyanti Frida Maryati Yusuf Harista Agustina HASAN, ANI M. Hayatiningsih Gubali Herinda Mardin Husain, Ilyas Ilyas Husain Ilyas Husain Ilyas Husain Indra Domili Irsan, Mohamad Ismail, Lilis U. Jusna Ahmad Jusran Jusran Kristina Dwi , Wulandari LANGKAH SEMBIRING LANGKAH SEMBIRING Latifa Tangkudung Ledy Mutmainah Y Syahril Lilan Dama Magfirahtul Jannah Mamu, Hartono Margaretha Solang Masra Latjompoh Moha, Tri Vinesa Mohamad Ikbal Riski Danial Muh Ikram Nur Muh. Nur Akbar Muhammad Isra Muhammad Isra Muhlis Muhlis Mustaqimah, Nur Nazifah R Adam Noer Fahnessa Dako Nona Wingti Posangi Novian Swasono Hadi NOVRI YOULA KANDOWANGKO NOVRI YOULA KANDOWANGKO Novri Youla Kandowangko Novri Youla Kandowangko Nur Afni Machmud Nur'ain Marton Angio Nurdin . Nurfadhlia Djafar Nurhayati Bialangi Nurinda Umadji Nurul Fajryani Usman Pangalo, Paulus PENI ULFIANI Pobela, Juliani Putri, Tarissa Eka Radina I. Timumun Rahmatiya, Rahmatiya Ramli Utina Riskanarti K. Lihaawa Risnayanti R Juramang Rulan U. Mustapa Salma Puasa Sela Restia Tangahu Siti Ainun Mabuia Siti Humairah Eka Putri SORAYA FITRIA NASIR Sri Delviyanti Lihawa Sri Yulianti Hadji Suaiba, Zachrianty SUKARTI MOELJOPAWIRO Sukarti Moeljopawiro SUKARTI MOELJOPAWIRO Sukirman Rahim Syam S. Kumaji Syapril, Syapril Tadidik, Ulfaida H. Tjut S. Djohan TJUT SUGANDAWATY DJOHAN TJUT SUGANDAWATY DJOHAN Torana, Melanie Triska Wahyuni Dawit Vivien Novariana Vrilianika Vrilianika Wawan Pembengo Widya Rahmawaty Saman Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsi Din Uno Wirnangsi Din, Uno Wirnangsih D. Uno Yulianti Humokor Yuniyarti Hulu Yusriyah Yusriyah