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All Journal International Journal of Public Health Science (IJPHS) The Indonesian Biomedical Journal Nusantara Medical Science Journal Alami Journal (Alauddin Islamic Medical) Journal JKM (Jurnal Kebidanan Malahayati) Jurnal Riset Kesehatan Poltekkes Depkes Bandung Oksitosin : Jurnal Ilmiah Kebidanan UMI Medical Journal Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Indian Journal of Forensic Medicine & Toxicology IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN) Narra J Journal of Aafiyah Health Research (JAHR) Quality : Jurnal Kesehatan Jurnal Yoga Dan Kesehatan Jurnal Info Kesehatan Preventif : Jurnal Kesehatan Masyarakat
Bukhari, Agussalim
Universitas Hasanuddin
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Evaluation of an E. coli-expressed spike protein-based in-house ELISA system for assessment of antibody responses after COVID-19 infection and vaccination Nurisyah, Sitti; Iyori, Mitsuhiro; Hasyim, Ammar A.; Amru, Khaeriah; Itani, Kei; Nakamura, Kurumi; Zainal, Kartika H.; Halik, Handayani; Djaharuddin, Irawaty; Bukhari, Agussalim; Asih, Puji BS.; Syafruddin, Din; Yoshida, Shigeto; Idris, Irfan; Yusuf, Yenni
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1250

Abstract

Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) for antibody detection and to evaluate its performance. The system was validated by comparing the in-house ELISA results with those obtained using a commercial ELISA with HEK293-expressed spike protein (H-S1). Recombinant SARS-CoV-2 spike protein was produced in E. coli, purified, and validated for antigenicity via ELISA. Indirect ELISAs with both E-S1 and H-S1 antigens were performed on 386 serum samples from COVID-19 survivors, vaccinated individuals, and pre-pandemic controls collected at different time points. The E-S1 ELISA showed a statistically significant but weak correlation with H-S1 ELISA across all samples (r=0.205; p=0.0001). Stronger correlations were observed among vaccinated individuals with prior infection on day 90 (r=0.6017; p<0.001) and in naïve vaccine recipients on day 30 (r=0.5361; p=0.0003). Pre-pandemic sera from a rural population in Sumba Island exhibited high background reactivity in E-S1 ELISA, likely due to anti-E. coli antibodies, while urban pre-pandemic sera from Jakarta showed a stronger correlation with H-S1 ELISA. This suggests potential regional or immune background differences influencing assay performance. Although E-S1 retained antigenic properties, its diagnostic utility is limited by non-specific reactivity and reduced sensitivity compared to H-S1. In conclusion, E. coli expression systems may not be ideal for producing spike protein-based ELISA antigens specific to SARS-CoV-2. Alternative expression systems, such as human or baculovirus, could enhance diagnostic accuracy and specificity for COVID-19 antibody detection.
Pemanfaatan Produk Lokal Olahan Rebon sebagai Makanan Tambahan untuk Meningkatkan Kadar IGF-1 Pada Anak Stunting Usia 24 - 60 bulan Anton, Sri Sulistyawati; Bukhari, Agussalim; Erika, Kadek Ayu; Baso, Aidah Juliaty A; Anton, Anton; Syarif, Isymiarni
Jurnal Yoga dan Kesehatan Vol 8 No 1 (2025)
Publisher : UHN IGB Sugriwa Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25078/jyk.v8i1.4065

Abstract

Berbagai gangguan pertumbuhan dan perkembangan sering dikaitkan dengan hormon IGF-1. Anak stunting cenderung memiliki kadar IGF-1 yang rendah. Kuantitas dan kualitas dari asupan protein memiliki efek kadar hormon IGF-1. Udang rebon adalah makanan lokal yang mengandung banyak protein dan mineral gizi. Studi quasy eksperimen ini melibatkan 88 anak stunting berusia 24 hingga 60 bulan. Sampel ini dibagi menjadi kelompok intervensi (n=44) dan kontrol (n=44). Selama sembilan puluh hari, anak-anak dalam kelompok intervensi menerima produk olahan rebon sebagai makanan tambahan. Pemeriksaan kadar serum IGF-1 dilakukan dengan metode ELISA pada hari ke 0 dan 90 pada kedua kelompok. Terdapat peningkatan rerata kadar hormon IGF-1 pada kelompok intervensi yang jauh lebih tinggi (49.25 ng/mL) dibandingkan dengan kelompok kontrol (14.82 ng/mL). Terdapat perbedaan kadar IGF-1 yang signifikan pada kelompok intervensi (p=0.0001). Rebon bermanfaat untuk meningkatkan kadar IGF-1 karena mengandung nilai gizi yang baik untuk anak. Rebon sebagai pangan lokal dapat menjadi pangan alternatif dan terjangkau yang bergizi dan bermanfaat bagi anak.
Nutritional Therapy in Adult Hirschsprung Disease Post-Total Colectomy and Ileostomy with Severe Protein-Energy Malnutrition: A Case Report Faradilah, Andi; Christine Rogahang; Nurbaya Syam; Agussalim Bukhari
Alami Journal (Alauddin Islamic Medical) Journal Vol 9 No 2 (2025): JULY
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/alami.v9i2.60167

Abstract

Adult Hirschsprung disease, also known as congenital aganglionic megacolon, is a rare congenital disorder characterized by the absence of ganglion cells in the distal segment of the large intestine, diagnosed in adulthood. Impaired nutrient absorption due to the dysfunctional intestinal segment can lead to malabsorption and malnutrition. A 23-year-old female presented with severe protein-energy malnutrition after total colectomy surgery for suspected adult Hirschsprung disease. She had a history of chronic constipation from childhood, abdominal pain, and abdominal distension. She was referred to a clinical nutritionist 2 days post-surgery. The nutrition therapy started with oral and parenteral nutrition, gradually increasing macronutrient requirements based on clinical condition and gastrointestinal tolerance.  On the 12th day post-total colectomy surgery, she developed complications of obstructive ileus, requiring ileostomy. Laboratory tests showed signs of inflammation. Nutritional support for adult Hirschsprung disease focuses on meeting energy requirements, enhancing nutrient absorption after abdominal surgery, and minimizing post-operative inflammation.
The Influence Of Edutainment-Based Education On Nutrition Lactation, Involution Uteri And Lochea Against Increased Knowledge, Attitudes And Behavior Of Puerperal Mothers Karnely, Karnely; Bukhari, Agussalim; Hidayanty, Healthy; Daud, Nurpudji Astuti; Alasiry, Ema
JKM (Jurnal Kebidanan Malahayati) Vol 10, No 8 (2024): Volume 10,No.8 Agustus 2024
Publisher : Program Studi Kebidanan Fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkm.v10i8.17170

Abstract

Kembalinya alat-alat reproduksi seperti sebelum hamil membutuhkan kandungan gizi yang cukup bagi ibu. Makanan yang dikonsumsi ibu nifas harus bermutu, bergizi, dan cukup kalori. Dimana makanan yang dikonsumsi berguna untuk melakukan aktifitas, metabolisme, proses memproduksi ASI, sebagai ASI sendiri yang akan dikonsumsi oleh bayi untuk pertumbuhan dan perkembangan bayi, serta mempertahankan tubuh terhadap infeksi. Tujuan penelitian yaitu untuk mencari pengaruh edukasi berbasis edutainment tentang gizi laktasi, involusio uteri dan lokhea terhadap peningkatan pengetahuan, sikap dan perilaku ibu nifas. Metode Penelitian menggunakan Quasi Eksperimen dengan rancangan Non Equivalent Control Group Design. Di dalam desain ini observasi dilakukan sebanyak 2 kali yaitu sebelum dilakukan perlakuan dan sesudah dilakukan perlakuan. Pada kelompok intervensi dan kelompok kontrol masing-masing dilakukan pretest dan post test. Populasi dalam penelitian ini adalah seluruh ibu Nifas yang berada di RSKDIA Pertiwi dan RSKDIA Siti Fatimah Makassar sebanyak 5843 ibu nifas. Berdasarkan dari hasil perhitungan diatas didapatkan jumlah sampel sebesar 47 orang kelompok intervensi di RSKDIA Pertiwi Makassar dan 47 orang kelompok kontrol di RSKDIA Siti Fatimah Makassar dengan menggunakan teknik Purposive Sampling. Hasil penelitian menunjukkan bahwa terdapat pengaruh signifikan antara pengetahuan dengan edukasi berbasis edutainment tentang gizi laktasi, involusi uteri, dan lochea. Hasil penelitian menunjukkan bahwa terdapat pengaruh signifikan antara sikap dengan edukasi berbasis edutainment tentang gizi laktasi, involusi uteri, dan lochea. Hasil penelitian menunjukkan bahwa terdapat pengaruh signifikan antara perilaku dengan edukasi berbasis edutainment tentang gizi laktasi, involusi uteri, dan lochea. Saran kepada bidan yang bertugas di daerah khususnya di RSKDIA Pertiwi Makassar dan RSKDIA Siti Fatimah Makassar agar lebih meningkatkan kinerja serta perhatiannya dalam memberikan informasi tentang gizi laktasi, involusi uteri, dan lochea Kata Kunci : Edukasi, Gizi Laktasi, Involusio Uteri, Lokhea, Pengetahuan, Sikap dan Perilaku. ABSTRACT The return of reproductive organs before pregnancy requires sufficient nutritional content for the mother. The food consumed by the puerperal mother must be of high quality, nutritious, and sufficiently caloric. Where the food consumed is useful for activities, metabolism, and the process of producing breast milk, as its own milk that will be consumed by the baby for the growth and development of the baby, as well as defending the body against infections. The purpose of this study is to find the influence of edutainment-based education on lactation nutrition, uterine and lochia involution on increasing the knowledge, attitudes and behaviors of puerperal mothers. This study used the Quasi-Experimental design method with a Non-Equivalent Control Group Design. In this design, observations are carried out as many as 2 times, namely before treatment and after treatment. In the intervention group and the control group, pre-test and post-test were carried out, respectively. The population in this study was all Puerperal mothers who were at RSKDIA Pertiwi and RSKDIA Siti Fatimah Makassar as many as 5843 puerperal mothers. Based on the results of the calculations above, the number of samples was obtained by 47 intervention groups at RSKDIA Pertiwi Makassar and 47 control groups at RSKDIA Siti Fatimah Makassar using the Purposive Sampling technique. The results showed that there was a significant influence between knowledge and edutainment-based education about lactation nutrition, uterine involution, and lochia. The results showed that there was a significant influence between attitudes and edutainment-based education about lactation nutrition, uterine involution, and lochia. The results showed that there was a significant influence between behavior and edutainment-based education about lactation nutrition, uterine involution, and lochia. It is recommended to midwives who serve in the regions, especially at RSKDIA Pertiwi Makassar and RSKDIA Siti Fatimah Makassar to further improve their performance and attention in providing information about lactation nutrition, uterine involution, and lochia Key Words: Education, Lactation Nutrition, Uterine Involution, Lochia, Knowledge, Attitudes and Behaviors. 
Hubungan Kadar Trigliserida, Massa Tubuh, Lingkar Pinggang Dengan Derajat Premenstrual Syndrome: Relationship between Triglyceride Levels, Body Mass, Waist Circumference and Degree of Premenstrual Syndrome Wahyu, Sri; Bukhari, Agussalim; Chalid, Maisuri T; Ilmianti, Ilmianti
Journal of Aafiyah Health Research (JAHR) Vol. 4 No. 2 (2023): JULY-DECEMBER
Publisher : Postgraduate Program in Public Health, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52103/jahr.v4i2.1551

Abstract

ABSTRAK Premenstrual syndrome (PMS) Sekumpulan gejala berupa gangguan fisik dan mental, dialami 7-10 hari menjelang menstruasi dan menghilang beberapa hari setelah menstruasi. Penelitian ini dilakukan untuk mengetahui hubungan kadar Trigliserida, Indeks Massa Tubuh (IMT) & Lingkar Pinggang dengan derajat premenstrual syndrome. Desain penelitian ini menggunakan pendekatan cross sectional study menggunakan teknik purposive sampling untuk mendapatkan sampel sebanyak 30 orang wanita usia subur. Data dianalisis dengan menggunakan uji pearson. Hasil penelitian menunjukan pada derajat premenstrual syndrome ringan kadar trigliserida mean±stdr deviasi (99.9±31.84),sedangkan premenstrual derajat sedang mean±stdr deviasi (78.8±24.61) dengan nilai korelasi 0.217(p>0.05).Pada IMT, pada derajat Premenstrual ringan IMT mean±stdr deviasi (22.17±4.72) derajat premenstrual Sedang IMT mean±stdr deviasi (25.08±3.02) dengan nilai korelasi 0.034 (p<0.05). Sedangkan berdasarkan lingkar pinggang pada derajat Premenstrual ringan LP mean±stdr deviasi (77.8±9.52), dan premenstrual sedang LP mean ±stndr deviasi (85.5±8.09) dengan nilai korelasi 0.118 (p>0.05). Dengan demikian dapat disimpulkan bahwa responden dengan Indeks massa tubuh obesitas beresiko mengalami premenstrual syndrome. ABSTRACT Premenstrual syndrome (PMS) A set of symptoms of physical and mental disorders, experienced 7-10 days before menstruation and disappears a few days after menstruation. This study examined the association levels of triglycerides, body mass index (BMI) and Waist Circumference with a degree of premenstrual syndrome. The study design was cross sectional study approach using purposive sampling techniques to obtain a sample of 30 women of childbearing age. Data were analyzed using Pearson test. The results showed the degree of mild premenstrual syndrome triglyceride levels stdr mean ± deviation (99.9 ± 31.84), whereas moderate premenstrual stdr mean ± deviation (78.8 ± 24.61) with the 0217 correlation values (p> 0.05). At IMT, the degree of mild premenstrual IMT stdr mean ± deviation (22:17 ± 4.72) degrees of premenstrual Medium stdr IMT mean ± deviation (8.25 ± 3.02) with the 0034 correlation values (p <0.05). While based on waist circumference in a mild degree of Premenstrual LP stdr mean ± deviation (77.8 ± 9:52), and premenstrual are mean ± stndr LP deviation (85.5 ± 8:09) with the 0118 correlation values (p> 0.05) Thus we can conclude that respondents with a mass index body obesity are at risk of premenstrual syndrome.
Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study Makmun, Armanto; Bukhari, Agussalim; Taslim, Nurpudji Astuti; Aminuddin, Aminuddin; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3250

Abstract

BACKGROUND: The prevalence of obesity, or an excessive fat accumulation, is keep increasing. In obesity, inflammation can be induced by leaky gut due to the intestinal tight junction barrier dysfunction. Zonula occludens-1 (ZO-1) plays a role in developing intestinal tight junction barrier dysfunction and gut microbiota imbalance, thus promote the translocation of bacterial endotoxin characterized by lipopolysaccharide (LPS) into circulation. Black rice extract (BRE) has been known to have anti-inflammatory property. This study was conducted to investigate the effect of BRE on body weight (BW), waist circumference (WC), body mass index (BMI), ZO-1 and LPS of obese patients.METHODS: Twenty-three male subjects were divided into non-obese group (NOG), obese group (COG) and BRE-obese group (BOG). Subjects in BOG received a daily dose of 5.6 g/day BRE for 4 weeks. BW, WC and BMI, serum ZO-1 and LPS were measured before and after treatment.RESULTS: BRE was prepared successfully and free from microbial contamination. Treatment of BRE for 4 weeks reduce BW (95.40±5.78 vs. 94.59±6.00 kg, p=0.043), WC (109.25±3.55 vs. 107.50±3.46 cm, p=0.000) BMI (32.65±1.86 vs. 32.18±1.80, p=0.000) and LPS (222.27±38.63 vs. 131.63±9.70 ng/mL, p=0.020) of obese subjects. The pre-post ZO-1 levels in all groups were not significantly different (p>0.05).CONCLUSION: Treatment of 5.6 gr BRE daily for four weeks can reduce BW, WC, BMI and serum LPS, but not serum ZO-1 in obese patients. Therefore, BRE may reduce inflammation in obesity.KEYWORDS: black rice, obesity, BW, WC, BMI, LPS, ZO-1
Application of Self-Reflection Using Reflective Journal on Oxytocin Massage Skills in Diploma Three Midwifery Students Arbie, Rina Sulisthia; Budu, Budu; Ahmad, Mardiana; Bukhari, Agussalim; Alasiry, Ema; Usman, Andi Nilawati
JURNAL INFO KESEHATAN Vol 21 No 2 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss2.1180

Abstract

Midwifery is a profession based on women-centered and evidence-based care through the provision of mutually respectful professional relationships and partnerships. To train students' skills, repeated learning is needed, learning can be sourced from experiences such as doing self-reflection by keeping a reflective journal. This research aims to analyze the effect of applying self-reflection using a reflective journal on oxytocin massage skills. This research is an experimental research using a one-group pretest-posttest group design approach, taking samples using a simple random sampling technique. The research population was all level III students in a bivariate analysis using the Friedman ANOVA test. The results showed that the pretest mean value was 59.28 increasing to 79.50 in the first posttest and 83.38 in the second posttest with a p-value <0.05, which means that there is an effect of applying reflective journals using reflective journals on oxytocin massage skills. Having students write reflective journals is a great way for them to learn new concepts. This approach is also useful for lecturers to get feedback on the concepts being studied by students. The use of reflective journals will guide students to analyze what they have and do not know about the material being studied and how to overcome the obstacles encountered so that learning goals are achieved and foster independent learning in students.
Kadar 8-Oxo-7, 8-Dihydro-2’-Deoxyguanosine (8-Oxo-Dgsn) Dalam Urine Sebagai Biomarker Penuaan Pada Pasien Tb Paru Wijaya, Cathrine Meryani; Santoso, Arif; Hamid, Firdaus; As’ad, Suryani; Bukhari, Agussalim; Zainuddin, Alfian
Preventif : Jurnal Kesehatan Masyarakat Vol. 12 No. 2 (2021): Volume 12 No.2 (2021)
Publisher : Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/preventif.v12i2.448

Abstract

Proses penuaan merupakan proses fisiologis yang tidak dapat dihindari. Pengukuran 8-oxo-dGsn dalam urin berpotensi sebagai cara baru untuk mengevaluasi proses penuaan dalam menentukan dan memperkirakan usia fisiologis. Penelitian ini betujuan untuk mengetahui kadar 8-Hydroxy-deoxyguanosine (8-OHdG) dalam urin sebagai biomarker penuaan pada pasien tuberculosis paru. Penelitian ini menggunakan pendekatan case control yakni penderita TB paru sebagai kelompok kasus dan kelompok orang sehat sebagai control dengan jumlah sampel sebanyak 50 orang (25 orang kasus dan 25 orang kontrol). Data dianalisis dengan menggunakan SPSS 21.0 (SPSS, Inc. Chicago, IL) dengan tingkat kemaknaan α=0.05.Hasil penelitian menunjukkan bahwa Kadar 8-Hydroxy-deoxyguanosine (8-OHdG) dalam urin sebagai biomarker penuaan pada kelompok kasus rata-rata 3.21±0.88. Kadar 8-Hydroxy-deoxyguanosine (8-OHdG) dalam urin sebagai biomarker penuaan pada kelompok kontrol rata-rata 2.38±0.71. Ada korelasi positif antara umur dengan kadar 8-Hydroxy-deoxyguanosine (8-OHdG) dalam urin sebagai biomarker penuaan (p < 0,001), dimana semakin bertambah umur maka kadar 8-Hydroxy-deoxyguanosine (8-OHdG) semakin tinggi. Terdapat perbedaan yang bermakna (p:0.001) secara statistik kadar 8-Hydroxy-deoxyguanosine (8-OHdG) sebagai biomarker penuaan antara kelompok kasus dengan kelompok kontrol. Kesimpulan penelitian ini adalah Tuberculosis paru mempercepat proses penuaan yang ditandai dengan peningkatan kadar 8-Hydroxy-deoxyguanosine (8-OHdG) dalam urine sebagai biomarker penuaan.
Co-Authors A. Yasmin Syauki A. Yasmin Syauki Ahmad, Mardiana Alfina Baharuddin Aminuddin Aminuddin Aminuddin Aminuddin Amru, Khaeriah Andi Alfian Zainuddin Andi Asadul Islam Andi Hariati Bachtiar Andi Nilawati Usman Andi Syurma Sari Ismail Anton Anton Anton, Sri Sulistyawati Arbie, Rina Sulisthia Ari Andini Junaedi Arif Santoso Aryanti R Bamahri Aryanti R. Bamahry Asih, Puji BS. Asrini Safitri Asrini Safitri As’ad, Suryani Bachtiar, Andi Hariati Baharuddin Baharuddin Baso, Aidah Juliaty A Budu - Burhanuddin Bahar Christine Rogahang Citra Kesumasari Daud, Nurpudji Astuti Din Syafruddin Djaharuddin, Irawaty Ema Alasiry, Ema Faradilah, Andi Fatmawati Nur Husain Ferry Sandra Firdaus Hamid Haerani Rasyid Haerani Rasyid Halik, Handayani Hamid, Firdaus Hasyim, Ammar A. Healthy Hidayanty Hidayanty, Healthy Ibrahim Labeda Ilhamjaya Patellongi Ilmianti, Ilmianti IRFAN IDRIS Itani, Kei Iyori, Mitsuhiro Josephine Thewakan Kadek Ayu Erika Karnely, Karnely Khairuddin Djawad Luthfi, Muhammad Maisuri T Chalid, Maisuri T Makmun, Armanto Marcellus Simadibrata Mardiana Madjid Maria Ingrid Budiman Meta Mahendradatta Mia Miranda Mochammad Hatta Mochammad Hatta Muh. Nasrum Massi Nakamura, Kurumi Nanang Wiyono Natalia Rorrong Pamilangan Ni Luh Puti Herli Mastuti Nur Ashari Nurbaya Syam Nurbaya Syam Nurhadi, Asty A. Nurisyah, Sitti Nurpudji A. Taslim Nurpudji Astuti Taslim Prihantono Prihantono R Satriono R Sutrisno Rabiah Rabiah Rahmani, Muhammad Z. Ratnaeni Rima January Rina Masadah Rinda Nariswati Rosdiana Natzir Rosdiana R Sri Ramadhany Sri Wahyu St.Pasriany Suryani As’ad Suryani As’ad Syarif, Isymiarni Taslim, Nurpudji Astuti Tawali, Suryani Tjahjadi Robert Tedjasaputra Umrayani Veni Hadju Veni Hadju Warmayana, I Gede Agus Krisna Wijaya, Cathrine Meryani Yoshida, Shigeto Yusuf Yenni Zainal, Kartika H. Zainuddin, Alfian