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All Journal International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Buletin Veteriner Udayana

Ni Luh Eka Setiasih

Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 376659

Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Hubungan Berat Badan dengan Berat Hati serta Gambaran Histologi Hati Broiler yang Diberikan Tepung Maggot Damara, Doni; Berata, I Ketut; Ardana, Ida Bagus Komang; Setiasih, Ni Luh Eka; Sulabda, I Nyoman
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.714

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung maggot Black Soldier Fly (Hermetia illucens) sebagai pakan tambahan pada broiler (Gallus domesticus) terhadap berat badan, berat dan struktur histologi hati. Broiler yang digunakan sebanyak 24 ekor dibagi ke dalam empat kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) masing-masing P0=0%, P1=1%, P2=2% dan P3=3% tepung maggot dalam pakan. Penelitian dilakukan selama 21 hari, broiler berumur 14- 35 hari. Parameter yang diteliti meliputi berat badan, berat dan struktur histologi hati yang diambil pada akhir penelitian. Analisis data untuk berat badan dan berat hati dilakukan dengan ANOVA dan dilanjutkan dengan uji Duncan. Untuk mengetahui hubungan antara keduanya dilakukan uii regresi. Analisis terhadap struktur histologi hati dilakukan dengan uji Kruskal Wallis. Hasil penelitian menunjukkan bahwa pemberian tepung maggot 1%, 2%, dan 3% menyebabkan berat badan dan berat hati broiler lebih rendah dibandingkan kontrol. Hasil uji regresi.menunjukkan terdapat hubungan yang kuat antara berat badan dengan berat hati. Pada pemeriksaan histologi hati menunjukkan terjadi degenerasi melemak pada hepatosit baik pada kelompok kontrol maupun perlakuan, serta tidak terjadi perbedaan nyata antara kelompok perlakuan. Dapat disimpulkan bahwa pemberian tepung maggot dalam pakan dapat menurunkan berat badan dan hati broiler. Ada hubungan kuat antara berat badan dengan berat hati. Tidak ada perbedaan gambaran histologi hati broiler antara perlakuan dengan kontrol.

Fluktuasi Profil Hematologi Tikus Putih Hewan Model Fibrosarkoma yang Diinduksi Benzo(a)piren Dewi, I Gusti Ayu Mirah Afsari; Adi, Anak Agung Ayu Mirah; Setiasih, Ni Luh Eka
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.267

Benzo(a)piren merupakan salah satu golongan bahan kimia yang memiliki sifat karsinogen sehingga mampu memicu pertumbuhan kanker dan seringkali digunakan untuk menginduksi tumor fibrosarkoma pada jaringan lunak hewan percobaan. Pemeriksaan hematologi merupakan indikator penting pada proses karsinogenesis atau tumorigenesis pada induksi senyawa benzo(a)piren. Penelitian ini bertujuan untuk mengetahui profil hematologi dari tikus putih sebagai hewan model fibrosarkoma yang diinduksi dengan senyawa karsinogen benzo(a)piren. Penelitian ini menggunakan 12 ekor tikus putih dengan dua perlakuan yaitu perlakuan 0 (P0) sebagai kontrol dan perlakuan 1 (P1) sebagai kontrol positif diinduksi dengan senyawa benzo(a)piren 0,3% yang dilarutkan dalam oleum olivarum 0,1 mL. Pengambilan sampel darah dilakukan tiga kali pada minggu ke-2, minggu ke-5 dan minggu ke-20 melalui sinus orbitalis dan diuji dengan pemeriksaan darah lengkap serta pembuatan preparat ulas darah. Hasil penelitian dari minggu ke-2, minggu ke-5 dan minggu ke-20 menunjukkan kisaran rerata pada total eritrosit (4,07-8,38)x106/µL, hemoglobin 8,03-14,8 g/dL, hematokrit 20,67-40%, total leukosit (3,67-12,4)x103/µL, neutrofil 16-48%, eosinofil 0-1,33%, basofil 0%, monosit 6-21,67%, dan limfosit 46-64%. Berdasarkan hasil penelitian dapat disimpulkan bahwa induksi senyawa karsinogen benzo(a)piren pada tikus putih penderita fibrosarkoma menunjukkan adanya fluktuasi pada profil hematologi.

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

VAKSINASI, EDUKASI DAN ELIMINASI ANJING LIAR SEBAGAI USAHA PERCEPATAN PENANGGULANGAN PENYAKIT RABIES DI BALI Kerta Besung. INK; NK. Suwiti; IK. Suatha; P. Suastika; IW. Piraksa; NL. Eka Setiasih
Buletin Udayana Mengabdi Vol 10 No 2 (2011): Volume 10 No.2 – September 2011
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (994.442 KB)

Rabies is an old zoonotic disease coused by lyssavirus of Rhabdovirus family, affecting warm-blooded animal including human. This community service is required to give information about the rabies diseases, vaccination and elimination of stray dogs. This activities was cooperated with Department of Animal Husbandry in Denpasar, Badung, Gianyar and Tabanan regency. The method used in this activities were to give information about the rabies diseases, to give vaccination and elimination of stray dogs. The number of dog vaccinated is: 172, eliminated : 81, and the activity was attended by 348 people, all of them were interested in this program. This activity should be continued, because when the rabies disease outbursts, it is very difficult to eradicate.

MENINGKATKAN PRODUKTIVITAS SAPI BALI MELALUI PELAYANAN KESEHATAN DI DESA BELOK/ SIDAN KABUPATEN BADUNG N.L.E. Setiasih; I.K. Suatha; P. Suastika; D.N.D.I. Laksmi
Buletin Udayana Mengabdi Vol 18 No 1 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Penyakit menjadi salah satu kendala bagi peternak sapi bali untuk meningkatkan produktivitas ternak. Pelayanan kesehatan menjadi salah satu usaha yang dapat dilakukan untuk mengurangi kerugian akibat penyakit. Tujuan dilakukan pengabdian ini adalah meningkatkan produktivitas sapi bali melalui pelayanan kesehatan di Desa Belok/Sidan Kabupaten Badung. Pelayanan kesehatan yang diberikan meliputi spraying, pemberian obat cacing, vitamin dan antibiotik di dusun Sekarmukti dan Belok, Desa Belok/Sidan. Jumlah peternak yang berhasil disasar sebanyak 64 orang dengan jumlah sapi sebanyak 190 ekor. Pelayanan kesehatan ini berhasil dilakukan dan mendapat respon sangat baik dari peternak di Desa Belok/Sidan. Masyarakat petani peternak menginginkan kegiatan tersebut dilakukan secara rutin setiap tahunnya. Kata kunci : Sapi bali; pelayanan kesehatan, spraying; obat cacing, vitamin

UPAYA PENINGKATAN POPULASI TERNAK SAPI DENGAN LAYANAN KESEHATAN DAN PENYULUHAN N.L.E. Setiasih; L.G.S.S. Heryani; P. Suastika; D.N.D.I. Laksmi; I.M. Dharmika
Buletin Udayana Mengabdi Vol 20 No 1 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Tujuan pengabdian ini adalah menunjang peningkatan populasi ternak sapi bali di Desa Belok Sidan Kabupaten Badung melalui pelayanan kesehatan. Daerah ini dipilih karena merupakan salah satu sentra pengembangan ternak sapi bali yang sangat potensial di Kabupaten Badung. Program pengabdian ini merupakan program lanjutan yang telah dilakukan sebelumnya, sehingga program pemerintah untuk merealisasikan swasembada daging sapi nasional segera dapat terealisasi. Pengabdian kepada masyarakat ini dilakukan dengan memberikan pelayanan kesehatan dan disertai dengan penyuluhan tata cara menjaga kesehatan ternak yang baik. Pelayanan kesehatan yang diberikan meliputi: spraying, pemberian obat cacing, vitamin, antibiotik, dan pemeriksaan kebuntingan di dusun Bon, Desa Belok/Sidan. Jumlah peternak yang berhasil disasar sebanyak 49 orang, masing-masing 24 ekor jantan dan 98 ekor betina Pelayanan kesehatan ini berhasil dilakukan dan mendapat respon sangat baik dari peternak di Desa Belok/Sidan. Masyarakat petani peternak menginginkan kegiatan tersebut dilakukan secara rutin setiap tahunnya.

Struktur Histologi dan Histomorfometri Kulit Anjing Ras Kintamani Asal Bali Ni Made Ayu Kurniawati; Ni Luh Eka Setiasih; Putu Suastika
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Kintamani dog is the only Balinese breed has been recognized by Federarion of International Cynology (Fédération Cynologique Internationale). Kintamani dogs live around Sukawana Village, KintamaniDistrict, Bangli Regency, of Bali. The purpose of this study was to determine the hystologycal structure and histomorphometry of kintamani dog skin. The kintamani dog used in this study were 1-2 years old.Histological structure observed by carlzeiss teaching microscope with objective lens magnification 5, 10, 20, 40, and 100x. In this study, the hystologycal structure of kintamani dog consists of the epidermis, dermis, and hypodermis, except on planum nasale. Planum nasale of kintamani dogs do not havehypodermis. The other components found in the hystologycal structure is sebaseous gland, sweat gland, hair follicles and blood vessels. While the histomorphometry of kintamani dog skin have a different thickness depending on location of the body and gender. In this study, Kintamani dog skin is the thickest in female planum nasale with thickness from epidermis to dermis at 6437.040 ?m and the thinnest inmale stomach at 2047.378 ?m.

Urinalisis Menggunakan Dua Jenis Dipstick (Batang Celup) pada Sapi Bali Iwan Harjono Utama; Evi Marieti Hutagalung; I Wayan Puspa Ari Laxmi; I Gusti Made Krisna Erawan2,; Sri Kayati Widyastuti; Luh Eka Setiasih; Ketut Berata
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.756 KB)

This study aimed to compare two kind of urinalysis dipsticks normaly used for human, for used in balicattle. A total of 100 urine samples were collected from 78 bulls and 22 cows ages between 4-7 years old.Each sample was kept in a plastic bottle and labelled until urinalysis was performed. Both dipstickshowed different urin chemical analysis results, except for nitrite. However, in general both dipsticksshowed consistency urinalysis results. Therefore, the two dipsticks can be used for urinalysis in balicattle.

Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih Hanifah Alshofa Nurul Aini; Desak Nyoman Dewi Indira Laksmi; Ni Luh Eka Setiasih; Steven Dwi Purbantoro
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicleswere measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, andred oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number ofantral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.

Keterkaitan antara Turbiditas Serum dan Laju Endap Darah dengan Kerusakan Hati pada Sapi Bali Iwan Harjono Utama; Yanne Yanse Rumlaklak; Dewa Ayu Dwita Karmi; Anak Agung Sagung Kendran; Sri Kayati Widyastuti; I Ketut Berata; Luh Eka Setiasih
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.559 KB)

This research was aimed to observe serum turbidity and blood sedimentation rate (ESR) as a predictorof hepatic damage in bali cattle. Two hundred whole blood and sera from 80 male and 120 female bullswere sampled from Mambal abbatoir, Badung, Bali. All blood were examined for their ESR and sera werefor their turbidity using ZnSO4 solution, besides we observed some hepatic damages pathology anatomicallywithout incision such as : internal bleeding, formation of connective tissue, swelling, and bile-ductenlargement. all of those damages were scaled graded from 0 (without abnormality) to 4 (more than 75%liver surface has abnormalities). Results showed all 86% bulls (male 32% and female 54%) have their seraturbidity ranged from 1,01-2,00, besides, 89% bulls (45% male and 44% female) have their ESR rangedfrom 3-8 mm in 24 hours. Most of liver abnormalities were : swelling (58%), bile-duct enlargement (50,5%),connective tissue formation (73%), and bleeding (59%) most of them were falling in scale 1 (less than 25%of liver surface area). Also, ESR has positive correlation (P<0,05) with connective tissue formation andserum turbidity value could be used to predict internal bleeding and connective tissue formation. It couldbe concluded ESR value could be used as a predictor of connective tissue formation and serum turbidityvalue could be used as a predictor for internal bleeding and connective tissue formation in bali cattle.

Co-Authors Anak Agung Gde Arjana Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anastasia Bhala Andika Diko Septiyatma Apriani, Ni Made Nina Astini, Ni Putu Sri Ayu Baiq Renny Kamaliani Brahma Tusta Bhirawa Budiartawan, I Komang Alit Cahyani, Luh Made Maha Citra Widiawati D.N.D.I. Laksmi Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Defi Lega Nurwidana Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Dewa Ayu Dwita Karmi Dewi, I Gusti Ayu Mirah Afsari DWI SURYANTO Elisabeth Karina Eristiawan, I Gede Erick Ersawati, Neti Evi Marieti Hutagalung Ginting, Regina Bonifasia Br Gunawati, Luh Sri Gusti Ayu Yuniati Kencana Hanifah Alshofa Nurul Aini Heriyani, Luh Gede Sri Surya I Gde Andhika Putra Pratama I Gede Erik Juliarta I Gede Soma I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Kadek Ari Satria Prayoga I Kadek Pradhana Putra I Ketut Berata I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Sumadi I Made Bayu Pandia Yudha Bauer I Made Kardena I Made Merdana I Made Sukada I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sampurna I Putu Sandika Arta Guna I Putu Suastika I Wayan Piraksa I Wayan Puspa Ari Laxmi I Wayan Sudira I Wayan Wirata I.K. Suatha I.M. Dharmika Ida Ayu Adhistania Pidada Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Indrawati Sendow Inna Narayani IW. Piraksa Iwan Harjono Utama Kamaliya Alawiyah Yahya Ketut Berata Kevin Dominika Luh Gde Sri Surya Heryani Luh Made Sudimartini Luh Made Sudimartini Maratun Janah Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Muhsi, Ach Moh Abd Ngurah Arbi Kencana Ngurah Intan Wiratmini Ni Kadek Eka Widiadnyani Ni Ketut Suwiti Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Putu Agustini Ni Made Ayu Kurniawati Ni Made Ayu Suastami Ni Made Riska Adnyani Ni Made Santi Rahayu Adiari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Nurul Amira Oktavyan Loys Mami P. Suastika Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Putra, I Komang Susila Semadi Putri Utami Putri Yuliana Mangindaan Putu Ayu Santika Putu Henrywaesa Sudipa Putu Suastika Raodatul Jannah Robertino Ikalinus, Robertino Samsuri Samsuri Samsuri Samsuri Setiyowati, Asri Sisyawati Putriningsih Sri Kayati Widyastuti Sri Kayati Widyastuti Stanislaus Valens Miten Larantukan Steven Dwi Purbantoro Teja, Putu Tessa Hariys Septianda Tjokorda Sari Nindhia Tri Ulfah Arema Yanti Umi Reston Vaswani Samaria Napitupulu Waskitha, Melati Pusparini Winda Ara Yulisa Wuriandari, Paula Bintang Yanne Yanse Rumlaklak Yogiana, Wayan Yoviniani Narti Dosom Yulia Khalifatun Nissa Zumara Mufida Hidayati

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