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All Journal Jurnal Sain Veteriner International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Indonesia Medicus Veterinus Buletin Veteriner Udayana

Ni Luh Eka Setiasih

Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 376659

Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Hubungan Berat Badan dengan Berat Hati serta Gambaran Histologi Hati Broiler yang Diberikan Tepung Maggot Damara, Doni; Berata, I Ketut; Ardana, Ida Bagus Komang; Setiasih, Ni Luh Eka; Sulabda, I Nyoman
Indonesia Medicus Veterinus Vol 10 (5) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.5.714

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung maggot Black Soldier Fly (Hermetia illucens) sebagai pakan tambahan pada broiler (Gallus domesticus) terhadap berat badan, berat dan struktur histologi hati. Broiler yang digunakan sebanyak 24 ekor dibagi ke dalam empat kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) masing-masing P0=0%, P1=1%, P2=2% dan P3=3% tepung maggot dalam pakan. Penelitian dilakukan selama 21 hari, broiler berumur 14- 35 hari. Parameter yang diteliti meliputi berat badan, berat dan struktur histologi hati yang diambil pada akhir penelitian. Analisis data untuk berat badan dan berat hati dilakukan dengan ANOVA dan dilanjutkan dengan uji Duncan. Untuk mengetahui hubungan antara keduanya dilakukan uii regresi. Analisis terhadap struktur histologi hati dilakukan dengan uji Kruskal Wallis. Hasil penelitian menunjukkan bahwa pemberian tepung maggot 1%, 2%, dan 3% menyebabkan berat badan dan berat hati broiler lebih rendah dibandingkan kontrol. Hasil uji regresi.menunjukkan terdapat hubungan yang kuat antara berat badan dengan berat hati. Pada pemeriksaan histologi hati menunjukkan terjadi degenerasi melemak pada hepatosit baik pada kelompok kontrol maupun perlakuan, serta tidak terjadi perbedaan nyata antara kelompok perlakuan. Dapat disimpulkan bahwa pemberian tepung maggot dalam pakan dapat menurunkan berat badan dan hati broiler. Ada hubungan kuat antara berat badan dengan berat hati. Tidak ada perbedaan gambaran histologi hati broiler antara perlakuan dengan kontrol.

Fluktuasi Profil Hematologi Tikus Putih Hewan Model Fibrosarkoma yang Diinduksi Benzo(a)piren Dewi, I Gusti Ayu Mirah Afsari; Adi, Anak Agung Ayu Mirah; Setiasih, Ni Luh Eka
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.267

Benzo(a)piren merupakan salah satu golongan bahan kimia yang memiliki sifat karsinogen sehingga mampu memicu pertumbuhan kanker dan seringkali digunakan untuk menginduksi tumor fibrosarkoma pada jaringan lunak hewan percobaan. Pemeriksaan hematologi merupakan indikator penting pada proses karsinogenesis atau tumorigenesis pada induksi senyawa benzo(a)piren. Penelitian ini bertujuan untuk mengetahui profil hematologi dari tikus putih sebagai hewan model fibrosarkoma yang diinduksi dengan senyawa karsinogen benzo(a)piren. Penelitian ini menggunakan 12 ekor tikus putih dengan dua perlakuan yaitu perlakuan 0 (P0) sebagai kontrol dan perlakuan 1 (P1) sebagai kontrol positif diinduksi dengan senyawa benzo(a)piren 0,3% yang dilarutkan dalam oleum olivarum 0,1 mL. Pengambilan sampel darah dilakukan tiga kali pada minggu ke-2, minggu ke-5 dan minggu ke-20 melalui sinus orbitalis dan diuji dengan pemeriksaan darah lengkap serta pembuatan preparat ulas darah. Hasil penelitian dari minggu ke-2, minggu ke-5 dan minggu ke-20 menunjukkan kisaran rerata pada total eritrosit (4,07-8,38)x106/µL, hemoglobin 8,03-14,8 g/dL, hematokrit 20,67-40%, total leukosit (3,67-12,4)x103/µL, neutrofil 16-48%, eosinofil 0-1,33%, basofil 0%, monosit 6-21,67%, dan limfosit 46-64%. Berdasarkan hasil penelitian dapat disimpulkan bahwa induksi senyawa karsinogen benzo(a)piren pada tikus putih penderita fibrosarkoma menunjukkan adanya fluktuasi pada profil hematologi.

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

VAKSINASI, EDUKASI DAN ELIMINASI ANJING LIAR SEBAGAI USAHA PERCEPATAN PENANGGULANGAN PENYAKIT RABIES DI BALI Kerta Besung. INK; NK. Suwiti; IK. Suatha; P. Suastika; IW. Piraksa; NL. Eka Setiasih
Buletin Udayana Mengabdi Vol 10 No 2 (2011): Volume 10 No.2 – September 2011
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (994.442 KB)

Rabies is an old zoonotic disease coused by lyssavirus of Rhabdovirus family, affecting warm-blooded animal including human. This community service is required to give information about the rabies diseases, vaccination and elimination of stray dogs. This activities was cooperated with Department of Animal Husbandry in Denpasar, Badung, Gianyar and Tabanan regency. The method used in this activities were to give information about the rabies diseases, to give vaccination and elimination of stray dogs. The number of dog vaccinated is: 172, eliminated : 81, and the activity was attended by 348 people, all of them were interested in this program. This activity should be continued, because when the rabies disease outbursts, it is very difficult to eradicate.

MENINGKATKAN PRODUKTIVITAS SAPI BALI MELALUI PELAYANAN KESEHATAN DI DESA BELOK/ SIDAN KABUPATEN BADUNG N.L.E. Setiasih; I.K. Suatha; P. Suastika; D.N.D.I. Laksmi
Buletin Udayana Mengabdi Vol 18 No 1 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Penyakit menjadi salah satu kendala bagi peternak sapi bali untuk meningkatkan produktivitas ternak. Pelayanan kesehatan menjadi salah satu usaha yang dapat dilakukan untuk mengurangi kerugian akibat penyakit. Tujuan dilakukan pengabdian ini adalah meningkatkan produktivitas sapi bali melalui pelayanan kesehatan di Desa Belok/Sidan Kabupaten Badung. Pelayanan kesehatan yang diberikan meliputi spraying, pemberian obat cacing, vitamin dan antibiotik di dusun Sekarmukti dan Belok, Desa Belok/Sidan. Jumlah peternak yang berhasil disasar sebanyak 64 orang dengan jumlah sapi sebanyak 190 ekor. Pelayanan kesehatan ini berhasil dilakukan dan mendapat respon sangat baik dari peternak di Desa Belok/Sidan. Masyarakat petani peternak menginginkan kegiatan tersebut dilakukan secara rutin setiap tahunnya. Kata kunci : Sapi bali; pelayanan kesehatan, spraying; obat cacing, vitamin

UPAYA PENINGKATAN POPULASI TERNAK SAPI DENGAN LAYANAN KESEHATAN DAN PENYULUHAN N.L.E. Setiasih; L.G.S.S. Heryani; P. Suastika; D.N.D.I. Laksmi; I.M. Dharmika
Buletin Udayana Mengabdi Vol 20 No 1 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Tujuan pengabdian ini adalah menunjang peningkatan populasi ternak sapi bali di Desa Belok Sidan Kabupaten Badung melalui pelayanan kesehatan. Daerah ini dipilih karena merupakan salah satu sentra pengembangan ternak sapi bali yang sangat potensial di Kabupaten Badung. Program pengabdian ini merupakan program lanjutan yang telah dilakukan sebelumnya, sehingga program pemerintah untuk merealisasikan swasembada daging sapi nasional segera dapat terealisasi. Pengabdian kepada masyarakat ini dilakukan dengan memberikan pelayanan kesehatan dan disertai dengan penyuluhan tata cara menjaga kesehatan ternak yang baik. Pelayanan kesehatan yang diberikan meliputi: spraying, pemberian obat cacing, vitamin, antibiotik, dan pemeriksaan kebuntingan di dusun Bon, Desa Belok/Sidan. Jumlah peternak yang berhasil disasar sebanyak 49 orang, masing-masing 24 ekor jantan dan 98 ekor betina Pelayanan kesehatan ini berhasil dilakukan dan mendapat respon sangat baik dari peternak di Desa Belok/Sidan. Masyarakat petani peternak menginginkan kegiatan tersebut dilakukan secara rutin setiap tahunnya.

HISTOPATHOLOGICAL PICTURE OF WHITE RATS HEART POST APPLICATION OF MIMOSIN FROM SIMPLISIA LAMTORO LEAF Ni Made Ayu Suastami; I Ketut Berata; Ni Luh Eka Setiasih; Samsuri; Luh Made Sudimartini; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p03

Mimosine is a toxic compound that can be found in the leaves of the lamtoro plant (Leucaena leucocephala). Toxicity that occurs in livestock depends on the concentration of mimosine in the feed and the length of time it is administered. This study aims to determine the histopathological picture of the heart of white rats (Rattus norvegicus) given mimosine from lamtoro leaf simplicia at different doses. The research used 2 months old Wistar male white rats weighing 300-350 grams. The 20 experimental animals used were divided into 4 treatment groups, namely P0 (negative control) without mimosine, P1 (positive control) given standard mimosine 5 mg/head/day, P2 given lamtoro leaf simplicia at a dose of 50 mg/head/day, and P3 simplicia lamtoro leaves at a dose of 150 mg/head/day. Mimosine was given orally for 14 days using a probe. On the 15th day, a necropsy was performed, the heart organ was removed and placed in 10% NBF. Next, it is processed to make histopathological preparations with HE staining. Histopathological examination was carried out including three variables: congestion, bleeding and necrosis. The severity of the lesion was scored, namely 0, 1, 2, and 3 respectively for normal, mild, moderate, and severe lesions. The data was then analyzed using the non-parametric Kruskal-Wallis and Mann-Whitney tests. The results of the study showed that administration of mimosine from lamtoro leaf simplicia could cause histopathology in the form of congestion, bleeding and necrosis lesions compared to controls and there was no effect on the difference in the dose of mimosine from lamtoro leaf simplicia between doses of 50 mg/head/day and 150 mg/head/day.

HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE I Kadek Ari Satria Prayoga; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p11

Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.

STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE I Gde Andhika Putra Pratama; Ni Luh Eka Setiasih; Sri Kayati Widyastuti; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p12

The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry

POTENTIAL OF MENIRAN LEAF EXTRACT ON NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS Citra Widiawati; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana; Ni Luh Eka Setiasih; Tjokorda Sari Nindhia; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p15

Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.

Co-Authors Anak Agung Gde Arjana Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anastasia Bhala Andika Diko Septiyatma Apriani, Ni Made Nina Astini, Ni Putu Sri Ayu Baiq Renny Kamaliani Brahma Tusta Bhirawa Budiartawan, I Komang Alit Cahyani, Luh Made Maha Citra Widiawati D.N.D.I. Laksmi Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Defi Lega Nurwidana Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Dewa Ayu Dwita Karmi Dewi, I Gusti Ayu Mirah Afsari Diza Pakom, Muhammad Sofi DWI SURYANTO Elisabeth Karina Eristiawan, I Gede Erick Ersawati, Neti Evi Marieti Hutagalung Ginting, Regina Bonifasia Br Gunawati, Luh Sri Gusti Ayu Yuniati Kencana Hanifah Alshofa Nurul Aini Heriyani, Luh Gede Sri Surya I Gde Andhika Putra Pratama I Gede Erik Juliarta I Gede Soma I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Kadek Ari Satria Prayoga I Kadek Pradhana Putra I Ketut Berata I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Sumadi I Made Bayu Pandia Yudha Bauer I Made Kardena I Made Merdana I Made Sukada i Nengah Wandia I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sampurna I Putu Sandika Arta Guna I Putu Suastika I Wayan Piraksa I Wayan Puspa Ari Laxmi I Wayan Sudira I Wayan Wirata I.K. Suatha I.M. Dharmika Ida Ayu Adhistania Pidada Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Indrawati Sendow Inna Narayani IW. Piraksa Iwan Harjono Utama Kamaliya Alawiyah Yahya Ketut Berata Kevin Dominika Luh Gde Sri Surya Heryani Luh Made Sudimartini Luh Made Sudimartini Maratun Janah Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Muhsi, Ach Moh Abd Ngurah Arbi Kencana Ngurah Intan Wiratmini Ni Kadek Eka Widiadnyani Ni Ketut Suwiti Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Putu Agustini Ni Made Ayu Kurniawati Ni Made Ayu Suastami Ni Made Riska Adnyani Ni Made Santi Rahayu Adiari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Nurul Amira Oktavyan Loys Mami P. Suastika Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Putra, I Komang Susila Semadi Putri Utami Putri Yuliana Mangindaan Putu Ayu Santika Putu Henrywaesa Sudipa Putu Suastika Raodatul Jannah Robertino Ikalinus, Robertino Samsuri Samsuri Samsuri Samsuri Setiyowati, Asri Sisyawati Putriningsih Sri Kayati Widyastuti Sri Kayati Widyastuti Stanislaus Valens Miten Larantukan Steven Dwi Purbantoro Teja, Putu Tessa Hariys Septianda Tjokorda Sari Nindhia TRI KOMALA SARI Tri Ulfah Arema Yanti Umi Reston Vaswani Samaria Napitupulu Waskitha, Melati Pusparini Winda Ara Yulisa Wuriandari, Paula Bintang Yanne Yanse Rumlaklak Yogiana, Wayan Yoviniani Narti Dosom Yulia Khalifatun Nissa Zumara Mufida Hidayati

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