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All Journal International Journal of Biosciences and Biotechnology Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Advances in Tropical Biodiversity and Environmental Sciences

Ni Luh Eka Setiasih

Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 376659

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Studi Morfologi dan Morfometri Duodenum Anjing Kintamani Andika Diko Septiyatma; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

The kintamani dog is a native dog from Bali that has an attractive and a gorgeous appearance. The aim of this research was to know the morphology and morphometry of kintamani dog's duodenum. This study used five female kintamani dogs. The observation of histologic morphology used a binocular light microscope with 5x, 10x, and 20x magnification. The results showed that length of duodenum is 16.2 ± 1.3 cm, and the width of duodenum are 3.1 ± 0.1 cm. The duodenal histology structure is composed of four layers: tunica mucosa, submucosa, muscularis, and serosa. Tunica mucosa thickness: 1,364.584 ± 255.504 mm, tunica submucosa: 360.136 ± 188.283 mm, tunica muscularis: 689.178 ± 267.228 mm, tunica serosa: 25.888 ± 11.93 mm.

Morfologi Ginjal Anjing Kintamani Betina I Kadek Pradhana Putra; Luh Gde Sri Surya Heryani; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Kintamani dog is a local mountain type dog that lives in Sukawana Village, Kintamani District, Bangli Regency, Bali. Research on the urinary system in Kintamani dog has never been done. This study aims to determine the morphology of anatomy and morphometrics of the kidney (ren) in Kintamani dog macroscopically and microscopically. This study used the kidney of 5 Kintamani dogs with an average age of 1-2 years old originating from the village of Sukawana. Histological observation based on Kiernan method and Hematoxylin-eosin (HE) staining. Weight measurement data, length, width, and renal thickness data were analyzed using Independent Sample T-test to compare the left and right kidney. Meanwhile, the anatomical and histological structure of the kidneys were analyzed descriptively qualitatively. The results showed that the left and right kidney weight was significantly different (P <0.05), while the length, width, and thickness were not significantly different (P> 0.05). Measurements of right renal morphometry, weight: 19.39 ± 4.24 g; length: 50.19 ± 2,43 mm; width: 29.57 ± 1.94 mm; cortex thickness: 6.17 ± 0.45 mm; medulla thickness: 8.48 ± 0.56 mm and pelvis thickness: 8.11 ± 1.29 mm. While the left kidney, weight: 20.51 ± 4.20 g; length: 50.88 ± 2,38 mm; width: 29.40 ± 1.65 mm; cortex thickness: 6.17 ± 0.31 mm; medulla thickness: 8.50 ± 0.49 mm and pelvis thickness: 8.95 ± 2.08 mm. Results of renal histomorphometry measurements, capsula thickness: 37.18 ± 5,67 mm; glomerulus area: 8.598,34 ± 1.277,06 mm; cortex thickness: 3.798,34 ± 603,54 mm and medulla thickness: 1.485,09 ± 286,92 mm.

STUDI HISTOLOGI USUS BESAR SAPI BALI Ni Ketut Suwiti; Ni Luh Eka Setiasih; I Putu Suastika; I Wayan Piraksa; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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A Study to detect the microscopic structure of large intestine (caecum, colon, rectum) ofbali cattle has been carried out. The samples were collected from 20 of bali cattle in Pesanggaran abattoir, Denpasar. The tissue samples were fixed, dehydrated and embeddedin paraffin and 4-5 mm sections. Harris-Haematoxilin-Eosin staining method, using toidentified of histological structure. Microscopic analysis was performed using binocularlight microscope (100 x, 450x). The study showed that the wall of the caecum, colon, andrectum are made up of four layers : mucosae, submucosae, muscularis externa, and serosa.The lined by mucosae ephitelium of simple columnar cells. The thick of mucosaare 29,9 ± 5,0 mm (caecum) 37,5 ± 8,4 mm (colon) and 58,9 ± 9,5 mm (rectum)respectively. The submucosae contain connective tissue and thick of these layerare 54,8 ± 8,8 mm; 21,2 ± 7,1 mm; and 16,5 ± 4,5 mm respectively. The muscularis externaconsists of two layers of smooth muscle inner circular and outer longitudinal. The thick ofthe mucularis externa are 98,5 ± 15,4 mm; 166,9 ± 44,4 mm; and 479 ± 28,6 mmrespectively. The serosa forms the outermost layer with thick 29,5 ± 8,5 mm;20,9 ± 7,6mm; and 12,1 ± 3,6 mm respectively. We observed for the presence richof Goblet cells and few limphatic nodules (Peyer’s patches) but no showed villi.

HISTOLOGICAL STRUCTURE OF THE KINTAMANI DOG ON THE SEXUAL MATURITY PERIOD Ni Luh Eka Setiasih; Putu Suastika; Luh Gede Sri Surya Heryani; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The objective of this study was to determine the histological structure of uterus of the kintamani dogs on the sexual maturity period. In this study using five samples of uterine organs of the kintamani dog. The tissue were taken from corpus of the uterine. The histological structure was observed using hematoxylin-eosin (HE). Identification of the histological structure of uterus observed with a microscope at 100x and 400x magnification. The results showed the endometrial lining consisted a single layer of the columnar epithelium and lamina propria with tubular glands, myometrium consisted of smooth muscle and perimetrium with connective tissue, nerves and blood vessels

Histomorfometri Granulosit Bibit Sapi Bali di Nusa Penida Oktavyan Loys Mami; Ni Ketut Suwiti; Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

The aim of this research were to find out the granulocyte histomorphometry of bali cattle in Nusa Penida. The sample are taken from 50 female blood bali cattle. Blood collection is carried out through the jugular vein using venoject. Making blood smears using the slide method was done at the sampling site, followed by fixation and staining of Giemsa. The measurement of granulocyte diameter was carried out using the Axio Imager Zeiss 2 microscope and ZEN 2012. software The data obtained were analyzed by descriptive statistics. The mean of granulocyte histomorphometry in Nusa Penida was 9.1±1,4 ?m neutrophils, 8.9±1,6 ?m eosinophils, and 8.9 ±1,7?m basophils with a range of 6-11 ?m in neutrophils, 5-13 ?m in eosinophils, and 5-11 ?m in basophils.

Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu Ni Ketut Suwiti; Putu Henrywaesa Sudipa; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to study the molecular weight and distribution of protein growth hormone receptors (GHR). The sample used was the Balinese beef eye ribs and Wagyu beef muscle. Molecular weight examination using the SDS-PAGE receptor method, Western Blotting, and immunohistochemistry staining. The results showed that the weight of the growth hormone receptor protein was 76 Kdalton in both types of cattle and the average score of IHK for Wagyu beef muscle was 234.5 and in Bali cattle was 199.6. It can be concluded, namely the weight molecule of the growth hormone receptor muscle ribs in both Wagyu and bali cattle with equal distribution.

Histological Structure of The Skin in The Thoracic and Abdominal Region of The Etawah Goats Cross Breed Ni Ketut Suwiti; Mergayanti Yudanta Eka Putri; Putu Suastika; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

There have been studies of the histological structure of male and female goat skins. The histological structure and histomorphometry were taken from two region of skin, which is the thorax and abdomen. The Samples were collected from 2-3 years old Etawah goat, and than was stained using a HarrisHematoxilin Eosin method. The data were analysis based on the region and sex. The results showed that the histological structure of Etawah cross breed consists of three layers were epidermis, dermis, and hipodermis respectively. The epidermis composed by stratum corneum, granulosum, spinosum, and basal. We were found keratin layer on the surface of epidermis. The dermis consists of papillare and reticulare stratum. That was found many sebaceous glands, sudorifera gland, pili muscle, hair follicles, blood vessels, and collagen fibers. The hypodermis layers of Etawah cross breed females skin mostly consists of the fatty tissue, whereas in males we have found thick and horizontally arranged connective tissue. The skin layer on male Etawah cross breed, thorax region thicker than the abdomen. The region and sex may have an effect on the histology structure and histomorphometry of Etawah cross breed skin.

Persentase Basofil, Eosinofil dan Neutrofil Sapi Bali yang Dipelihara dengan Pakan Berbasis Organik Putu Ayu Santika; Ni Ketut Suwiti; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

One of the Bali cattle breeding maintenance is organic-based feeding. Organic-based feed is likely to not be as optimal as the composition of feed like feed in general, such as minerals and other organic substances. The condition and composition of the feed can affect the number of blood leukocytes and cause changes in the percentage of leukocyte cells such as basophils, eosinophils and neutrophils. This study aims to determine the percentage of basophils, eosinophils, neutrophils in bali cattle blood based on organic feed. This study was carried out on a farm owned by residents of the Village of Bulian, Kubutambah District, Buleleng Regency. Samples in the form of blood taken from 10 female bali cattle by vein using venoject. Next, blood smear preparations were made and then fixed and stained by Harris Hematoxillin-Eosin (HE). Percentage of basophils, eosinophils, neutrophils calculated by cross-sectional method. Data obtained and analyzed with descriptive quantitative. The results showed that cattle raised with an organic based maintenance system experienced basophilia, eosinophilia, and neutropenia.

Penambahan Tepung Daun Kelor dalam Pakan Meningkatkan Berat Hati Tikus Putih Maria Natalia Dhiu Botha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to determine the addition of moringa flour (Moringa oleifera) in the feed to the weight of the liver of white rats (Rattus norvegicus). Moringa leaf flour was administered orally in 20 rats divided into 5 treatment groups with 0%, 2.5%, 5.0%, 10% and 20% concentration respectively with five replicates for one month. The white mouse was dissected and weighed heavily. This study used a Completely Randomized Design (RAL). The data obtained were analyzed using ANOVA (analysis of variance), and continued by Duncan Multiple Range Test. The results obtained were the addition of 20 ml of Moringa oleifera flour in the diet to increase the liver weight of white rat (Rattus norvegicus), while the addition of maize flour 10%, 5% and 2.5% did not affect to the white rat’s (Rattus norvegicus) liver weight.

The Gambaran Histologi dan Histomorfometri Limpa Kambing Peranakan Etawah Tri Ulfah Arema Yanti; Ni Ketut Suwiti; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The objective of this study to find out the histological and histomorphometry description of etawah crossbreed (PE) spleen. The spleens were collected from 10 male and female PE goats in Kampung Jawa slaughterhouse, Denpasar, Bali, then fixed in a solution of 10% Neutral Buffer Formalin processed for making preparation histology based on the method of Kiernan with hematoxylin-Eosin (HE) staining techniques. The goat's spleen consists of capsule, white and red pulp. Spleen histomorphometry of male and female PE goats obtained results, each having a capsule thickness of 128.58 ± 0.37 µm and 163.40 ± 0.35 ?m, trabecula thickness of 125.68 ± 0.13 µm and 131.45 ± 0, 28 µm, and white pulp diameter 466.63 ± 0.15 µm and 392.40 ± 0.30 µm respectively. It was concluded that gender affected the histology structure and histomorphometry of etawah crossbreed spleen.

Co-Authors Anak Agung Gde Arjana Anak Agung Sagung Kendran Anastasia Bhala Andika Diko Septiyatma Apriani, Ni Made Nina Astini, Ni Putu Sri Ayu Baiq Renny Kamaliani Brahma Tusta Bhirawa Budiartawan, I Komang Alit Cahyani, Luh Made Maha D.N.D.I. Laksmi Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Defi Lega Nurwidana Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Dewa Ayu Dwita Karmi Dewi, I Gusti Ayu Mirah Afsari DWI SURYANTO Elisabeth Karina Eristiawan, I Gede Erick Ersawati, Neti Evi Marieti Hutagalung Ginting, Regina Bonifasia Br Gunawati, Luh Sri Hanifah Alshofa Nurul Aini Heriyani, Luh Gede Sri Surya I Gede Erik Juliarta I Gede Soma I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Kadek Pradhana Putra I Ketut Berata I Ketut Suatha I Ketut Sumadi I Made Bayu Pandia Yudha Bauer I Made Kardena I Made Merdana I Made Sukada I Nyoman Suarsana I Nyoman Sulabda I Putu Sandika Arta Guna I Putu Suastika I Wayan Piraksa I Wayan Puspa Ari Laxmi I Wayan Sudira I Wayan Wirata I.K. Suatha I.M. Dharmika Ida Ayu Adhistania Pidada Ida Bagus Komang Ardana Ida Bagus Oka Winaya Indrawati Sendow Inna Narayani IW. Piraksa Iwan Harjono Utama Kamaliya Alawiyah Yahya Ketut Berata Kevin Dominika Luh Gde Sri Surya Heryani Luh Made Sudimartini Maratun Janah Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Muhsi, Ach Moh Abd Ngurah Arbi Kencana Ngurah Intan Wiratmini Ni Kadek Eka Widiadnyani Ni Ketut Suwiti Ni Luh Putu Agustini Ni Made Ayu Kurniawati Ni Made Riska Adnyani Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Nurul Amira Oktavyan Loys Mami P. Suastika Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Putra, I Komang Susila Semadi Putri Yuliana Mangindaan Putu Ayu Santika Putu Henrywaesa Sudipa Putu Suastika Raodatul Jannah Robertino Ikalinus, Robertino Samsuri Samsuri Setiyowati, Asri Sisyawati Putriningsih Sri Kayati Widyastuti Stanislaus Valens Miten Larantukan Steven Dwi Purbantoro Teja, Putu Tessa Hariys Septianda Tri Ulfah Arema Yanti Umi Reston Vaswani Samaria Napitupulu Waskitha, Melati Pusparini Winda Ara Yulisa Yanne Yanse Rumlaklak Yogiana, Wayan Yoviniani Narti Dosom Yulia Khalifatun Nissa Zumara Mufida Hidayati

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