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A STRUCTURE AND HISTOMORPHOMETRY OF BALI DUCK TESTES IN THE GROWER PHASE Ni Made Santi Rahayu Adiari; Ni Luh Eka Setiasih; I Putu Sampurna; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p17

Abstract

Testes are male reproductive organs which are responsible for producing spermatozoa and steroid hormones. This study aims to determine the histology and histomorphometry structure of bali duck testes in the grower phase at the age of 12 weeks in one of the farms in Mengwi District, Badung Regency. The number of samples used in this research was 10 male bali ducks that were in good health. There are two examination methods carried out, namely the qualitative descriptive anatomical structure and histology examination method and the quantitative histomorphometry examination method of bali duck testicles which are analyzed using the One-Sample T-test with a confidence level of 95%. The results of this study show the anatomical structure of the bali duck testicles which are located in the abdominal cavity, between the kidneys and the lungs. The pair of testicles have the shape of a bean and are cream colored. The results of research on the histological structure of bali duck testes consist of seminiferous tubules, interstitial tissue, leydig cells, and tunica albuginea. The results of histomorphometry measurements of bali duck testes showed that the wall thickness of the seminiferous tubules was 23.6760 ± 1.4425 (µm), the number of seminiferous tubules was 421.1000 ± 56.1396 and the thickness of the tunica albuginea was 29.5000 ± 5.048 (µm). Considering the importance of knowledge about the structure of bali duck testicles, it is recommended for future researchers to examine the structure and histomorphometry of bali duck testicles at different ages in the growing phase.
HISTOLOGY AND PROFILE OF AGRANULOCYTE OF BALI CATTLE WITH CONVENTIONAL REARED SYSTEM Putri Utami; Ni Ketut Suwiti; I Nyoman Suartha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p04

Abstract

Bali cattle are one of the livestock that are currently widely bred by farmers in Bali. Generally, Bali cattle are raised conventionally or traditionally and pay less attention to the health of the cattle. This situation can affect the immune system of Bali cattle, especially white blood cells that play a role in the immune system such as lymphocytes and monocytes. This study aims to determine the histomorphometry of white blood cells agranulocytes lymphocytes and monocytes in Bali cattle with conventional reared system. Blood test preparations were taken from 30 cows in Bulian Village, Kubutambahan Subdistrict, Buleleng Regency, Bali. Fixation and staining using MDT IndoReagen®. Measurements were made using the EP View application connected to a microscope with 1000x magnification. Calculation of the number of lymphocytes and monocytes by counting up to 100 white blood cells. The results showed that the histomorphology of lymphocytes has an irregular shape, a thin blue-purplish cytoplasm, with a nucleus that almost fills the cytoplasm. Lymphocytes have an area of 17.56 ± 2.37 µm2, perimeter of 14.7 ± 1.14 µm, and diameter of 4.71 ± 0.33 µm. The histomorphology of monocytes has an irregular shape, the cytoplasm is not granular and the nucleus is found hollow. Monocytes have an area of 22.59±5.09 µm2, perimeter of 16.69±2.37 µm, and diameter of 5.19±0.66 µm. Further research is needed on the histomorphometry of granulocytes: neutrophils, basophils, and eosinophils of Bali cattle.
HEPATIC HISTOPATHOLOGY OF WHITE RATS EXPOSED TO LEAD AND TREATED WITH MORINGA OLEIFERA LEAF SIMPLICIA Wuriandari, Paula Bintang; Berata, I Ketut; Winaya, Ida Bagus Oka; Kardena, I Made; Setiasih, Ni Luh Eka; Samsuri; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p18

Abstract

Lead (Pb) is a heavy metal that induces the formation of free radicals and causes hepatocellular damage, making the liver one of the primary target organs of lead toxicity. Moringa oleifera leaves contain natural antioxidants with the potential to counteract oxidative stress induced by lead exposure. This study aimed to evaluate the effect of Moringa oleifera leaf simplicia on liver histopathology in white rats (Rattus norvegicus) exposed to lead acetate. A total of 20 rats were randomly divided into four groups: P0 (negative control; standard feed and water), P1 (positive control; 0.5 mg/head/day of lead acetate for 3 days), P2 (0.5 mg/head/day of lead acetate plus 0.1 g/mL/day of Moringa oleifera leaf simplicia), and P3 (0.5 mg/head/day of lead acetate plus 0.6 g/mL/day of Moringa oleifera leaf simplicia). Treatments were administered for 14 days via intragastric gavage. On day 15, the rats were euthanized, and liver tissues were collected, processed histologically, and stained with hematoxylin-eosin. Histopathological lesions, including congestion, fatty degeneration, and necrosis, were scored on a scale from 0 to 3 based on severity. The Kruskal-Wallis test showed significant differences among groups for all lesions (P < 0.05). The Mann-Whitney test revealed significant differences in hepatic congestion between P0 and P1, P0 and P2, as well as between P3 and P1-P2, while no significant difference was observed between P0 and P3. For fatty degeneration and necrosis, P0 differed significantly from P1, P2, and P3. In conclusion, Moringa oleifera leaf simplicia at a dose of 0.6 g/mL/day reduced the severity of hepatic congestion caused by lead exposure; however, its effects on fatty degeneration and necrosis were not statistically significant.
Co-Authors Anak Agung Gde Arjana Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anastasia Bhala Andika Diko Septiyatma Apriani, Ni Made Nina Astini, Ni Putu Sri Ayu Baiq Renny Kamaliani Brahma Tusta Bhirawa Budiartawan, I Komang Alit Cahyani, Luh Made Maha Citra Widiawati D.N.D.I. Laksmi Damara, Doni Darmawan, I Gusti Ayu Chintya Darmayanti, Mahda Dwi Defi Lega Nurwidana Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Dewa Ayu Dwita Karmi Dewi, I Gusti Ayu Mirah Afsari DWI SURYANTO Elisabeth Karina Eristiawan, I Gede Erick Ersawati, Neti Evi Marieti Hutagalung Ginting, Regina Bonifasia Br Gunawati, Luh Sri Gusti Ayu Yuniati Kencana Hanifah Alshofa Nurul Aini Heriyani, Luh Gede Sri Surya I Gde Andhika Putra Pratama I Gede Erik Juliarta I Gede Soma I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Kadek Ari Satria Prayoga I Kadek Pradhana Putra I Ketut Berata I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Suatha I Ketut Sumadi I Made Bayu Pandia Yudha Bauer I Made Kardena I Made Merdana I Made Sukada I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Sampurna I Putu Sandika Arta Guna I Putu Suastika I Wayan Piraksa I Wayan Puspa Ari Laxmi I Wayan Sudira I Wayan Wirata I.K. Suatha I.M. Dharmika Ida Ayu Adhistania Pidada Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Indrawati Sendow Inna Narayani IW. Piraksa Iwan Harjono Utama Kamaliya Alawiyah Yahya Ketut Berata Kevin Dominika Luh Gde Sri Surya Heryani Luh Made Sudimartini Luh Made Sudimartini Maratun Janah Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Muhsi, Ach Moh Abd Ngurah Arbi Kencana Ngurah Intan Wiratmini Ni Kadek Eka Widiadnyani Ni Ketut Suwiti Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Putu Agustini Ni Made Ayu Kurniawati Ni Made Ayu Suastami Ni Made Riska Adnyani Ni Made Santi Rahayu Adiari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Nurul Amira Oktavyan Loys Mami P. Suastika Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Putra, I Komang Susila Semadi Putri Utami Putri Yuliana Mangindaan Putu Ayu Santika Putu Henrywaesa Sudipa Putu Suastika Raodatul Jannah Robertino Ikalinus, Robertino Samsuri Samsuri Samsuri Samsuri Setiyowati, Asri Sisyawati Putriningsih Sri Kayati Widyastuti Sri Kayati Widyastuti Stanislaus Valens Miten Larantukan Steven Dwi Purbantoro Teja, Putu Tessa Hariys Septianda Tjokorda Sari Nindhia Tri Ulfah Arema Yanti Umi Reston Vaswani Samaria Napitupulu Waskitha, Melati Pusparini Winda Ara Yulisa Wuriandari, Paula Bintang Yanne Yanse Rumlaklak Yogiana, Wayan Yoviniani Narti Dosom Yulia Khalifatun Nissa Zumara Mufida Hidayati