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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.) TERHADAP Propionibacterium acnes Syalsabila Putri; Ridwanto; Haris Munandar Nasution; Anny Sartika Daulay
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 2 No. 2 (2023): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.003 KB) | DOI: 10.32696/fjfsk.v2i2.1891

Abstract

Daun Mahkota Dewa merupakan tanaman asli indonesia yang berasal dari daerah Papua. Daun mahkota dewa di masyarakat sibolga biasa digunakam untuk mengobati berbagai penyakit secara alamiah. Penelitian ini bertujuan untuk mengetahui golongan senyawa kimia yang terkandung dalam daun mahkota dewa dan untuk mengetahui aktivitas antibakteri ekstrak etanol daun mahkota dewa terhadap bakteri Propionibacterium acnes. Ekstrak simplisia daun mahkota dewa dibuat dengan pelarut etanol 96% dengan metode maserasi. Ekstrak etanol daun mahkota dewa dibuat didalam berbagai konsentrasi yaitu 30%, 40%, 50%. Kontrol positif yang digunakan yaitu Tetraksiklin HCl dan kontrol negatif yang digunakan ialah DMSO. Ada beberapa pengujian yang dilakukan pada daun mahkota dewa yaitu skrining fitokimia, pemeriksaan makroskopik dan mikroskopik, pemeriksaan kadar air, kadar sari larut dalam air, kadar sari larut dalam etanol, kadar abu total, dan kadar abu tidak larut asam. Hasil penelitian yang dilakukan menunjukan bahwa ekstrak etanol daun mahkota dewa mengandung senyawa metabolit sekunder golongan alkaloid, flavonoid, saponin, tanin, steroid/triterpenoid yang memiliki aktivitas antibakteri. Berdasarkan hasil penelitian terhadap antibakteri menunjukkan bahwa ekstrak etanol daun mahkota dewa dapat digunakan untuk menghambat bakteri Propionibacterium acnes.Adapun hasil pada konsentrasi 30% berdiameter 8,03 mm dikategorikan sedang, pada konsentrasi 40% berdiameter 9,26 mm dikategorikan sedang, dan pada konsentrasi 50% berdiameter 9,73 mm dikategorikan sedang.
UJI STABILITAS WARNA BERDASARKAN INTENSITAS DAN KADAR KURKUMIN EKSTRAK KUNYIT DAN TEMULAWAK Nurul Shaleha; Anny Sartika Daulay
Cross-border Vol. 6 No. 2 (2023): JULI-DESEMBER
Publisher : Lembaga Penelitian Dan Pengabdian Masyarakat Institut Agama Islam Sultan Muhammad Syafiuddin Sambas Kalimantan Barat

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Abstract

Turmeric is one of the potential medicinal plants, apart from being a raw material for medicine, it is also used as a natural dye. Yellow dye (curcumin) is used as a food coloring for humans. Temulawak (Curcuma xanthorriza Robx) can be used as the main medicinal ingredient in supporting the provision of color. The purpose of this study was to determine the best storage time for turmeric and temulawak extracts that could be used as natural dyes based on color intensity and curcumin content. In this study, the manufacture of turmeric and temulawak extracts was carried out using the decoction method. Determination of storage variations for 7 days was carried out on the influence of storage temperature, namely room temperature (30 ), refrigerator temperature (7 ), drying cabinet temperature (32 ). The color absorbance measurement of curcumin content was carried out by visible spectrophotometric method. The results of the absorbance measurement were used to determine the color stability. The color stability of curcumin in the sample at variations in storage time and temperature was determined based on the absorbance represented in the color intensity of the sample. Furthermore, the color stability can be determined based on the curcumin content. The results of this study were obtained, the absorbance intensity test in turmeric was carried out at a wavelength of 425.14 nm with the results of 0.582 (day 1), temulawak had an absorbance of 0.463 (day 1). The color stability test changed on the 4th day at the temperature of the drying cabinet and room temperature, at the temperature of the refrigerator the color remained stable. Temulawak and turmeric extracts as natural dyes can be stored for a long time if stored at refrigerator temperature the color will remain stable.
PERBANDINGAN KADAR KURKUMIN DARI EKSTRAK KUNYIT DAN TEMULAWAK YANG DITENTUKAN DENGAN METODE SPEKTROFOTOMETRI VISIBLE Safrida Safrida; Anny Sartika Daulay
Cross-border Vol. 6 No. 2 (2023): JULI-DESEMBER
Publisher : Lembaga Penelitian Dan Pengabdian Masyarakat Institut Agama Islam Sultan Muhammad Syafiuddin Sambas Kalimantan Barat

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Abstract

Turmeric (Curcuma longa L.) is one of the plants known by the public as a spice that has many health benefits. Temulawak (Curcuma zanthorrhiza Roxb.) is one of Indonesia's rich spices that has been known for its benefits and properties since time immemorial. Curcumin is a natural chemical compound found in turmeric and ginger. The purpose of this study was to determine the levels of curcumin by using visible spectrophotometric methods from extracts of turmeric and temulawak using water as a solvent with the decoctation method. The extraction method chosen in this study is the decoctation method. Turmeric and temulawak were extracted by weight ratio of turmeric and solvent 1:3 at a temperature of 90ºC for 30 minutes. The results of this decoctation are separated by decantation. In the results of this liquid extract of turmeric and temulawak, a qualitative test of curcumin was determined using the thin layer chromatography (TLC) method with silica gel 60 F254 as stationary phase and chloroform: ethanol: glacial acetic acid (94: 5: 1) as mobile phase. Then the determination of curcumin levels in turmeric and temulawak extracts was carried out quantitatively using the visible spectrophotometric method. The results of the qualitative test using thin layer chromatography (TLC) obtained the value of curcumin Rf 0.54, turmeric extract Rf 0.54, temulawak extract Rf 0.54. The maximum wavelength obtained is 425.14 nm with an absorbance of 0.428. In determining the calibration curve, the regression equation y = 0.14114x-0.00452 is obtained with r = 0.99963. The levels of curcumin obtained using visible spectrophotometric methods are turmeric extract 80,91088± 1,83864 mg/gand temulawak extract 48,81070± 0,75803 mg/g.
OPTIMASI PEMBUATAN TEPUNG TALAS TERMODIFIKASI (Modified Taro Flour) DENGAN FERMENTASI Lactobacillus casei BERDASARKAN KADAR PROTEIN Imelda Natasia Br S Meliala; Anny Sartika Daulay; Ridwanto Ridwanto; Haris Munandar Nasution
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains (INPRESS)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1110

Abstract

Ketergantungan penggunaan tepung terigu dapat dikurangi dengan menggunakan sumber karbohidrat lainnya seperti talas yang merupakan pangan lokal. Lactobacillus casei adalah salah satu spesies Bakteri Asam Laktat yang dapat menggunakan selobiosa sebagai sumber nutrisinya. Diperkirakan Lactobacillus casei dapat mendegresi sel umbi talas agar meningkatkan kadar protein talas. Diantara faktor yang dapat mempengaruhi kondisi fermentasi untuk menghasilkan tepung termodifikasi yang optimal adalah waktu fermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh optimasi fermentasi umbi talas terhadap organoeleptis dan kadar protein pada tepung talas termodifikasi. Penelitian ini menggunakan metode eksperimental dengan dua optimasi waktu fermentasi yaitu fermentasi 48 jam dan fermentasi 72 jam dan uji karakterisasi yaitu uji organoleptis, uji kadar air, uji kadar abu dan uji kadar protein. Hasil penelitian ini menunjukkan organoleptis tepung mocaf, tepung talas, tepung talas termodifikasi berwarna coklat dan putih kecoklatan, bau sedikit asam dan tekstur yang halus. Kadar protein mocaf yaitu 2,17, tepung talas 2,33%, tepung talas termodifikasi 48 jam 3,16%, tepung talas termodifikasi 72 jam 3,53%. Kadar air tepung mocaf 11,31%, tepung talas 8,67%, tepung talas termodifikasi sebesar 10,81%. Kadar abu tepung mocaf 1,13%, tepung talas 2,73%, tepung talas termodifikasi 1,82%. Hasil yang diperoleh menunjukkan bahwa  fermentasi 72 jam merupakan hasil yang optimal.
Uji aktivitas antioksidan ekstrak etanol daun bunga melati (Jasminum sambac (L.) Sol. ex Aiton) dengan metode DPPH Selfiani Selfiani; M. Pandapotan Nasution; Anny Sartika. D; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.200

Abstract

Antioxidant compounds are substances that can absorb or neutralize free molecules so they can prevent degenerative diseases such as heart disease and cancer. Antioxidants are able to donate electrons to stop free radical chain reactions that can damage the body. Jasmine flower leaves contain bioactive substances such as flavonoids, alkaloids, and tannins which are potential ingredients as natural antioxidants. One method to measure or determine free radical scavenging antioxidants is the DPPH (1,1-Diphenyl-2-Picrylhidrazyl) method. This study aims to determine the antioxidant activity of the ethanol extract of jasmine leaves based on the IC value50. The methods used in this study included examination of simplicia characteristics, phytochemical screening and antioxidant activity tests using the DPPH method (1,1-Diphenyl-2-picrylhidrazyl) using a UV-Vis spectrophotometer. The results of this study revealed that the ethanol extract of jasmine flower leaves (Jasminum sambac (L.) Sol. ex Aiton) showed that the extract contained chemical compounds of alkaloids, flavonoids, saponins, tannins, and steroids. In addition, this study also revealed that the ethanol extract of jasmine leaves has antioxidant activity with an IC value50 namely 56.05 µg/mL and for vitamin C the IC value was obtained50 3.70 µg/mL. This shows that the ethanol extract of jasmine leaves positively contains secondary metabolites and has the potential as an antioxidant with a strong classification compared to vitamin C, which is an antioxidant with a very strong classification.
Penetapan kadar flavonoid total ekstrak etanol daun bunga melati (Jasminum sambac (L.) Sol. ex Aiton) dengan spektrofotometri Uv-Vis Hastri Kholifah; M. Pandapotan Nasution; Anny Sartika Daulay; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.201

Abstract

Traditional medicine uses plants with a natural ingredient content as its raw material. The bioactive compounds found in plants are secondary metabolites such as alkaloids, flavonoids, tannins, glycosides, steroids, and saponins. One of the plants containing secondary metabolite compounds is jasmine. This study aims to determine the chemical compounds contained in the ethanol extract and the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. The stages of this research included processing plant simplicia, making ethanol extract, examining characteristics, screening phytochemicals, and determining the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. Extract jasmine flower leaves by maceration method using 96% ethanol solvent; the extract obtained was concentrated using a rotary evaporator; then the total flavonoid content was determined using the UV-Vis spectrophotometry method. The research shows that the ethanol extract of jasmine flower leaves (Jasminum sambac (L.) Sol. ex Aiton) contains several secondary metabolite compounds, such as flavonoids, glycosides, alkaloids, saponins, steroids, and tannins. A number of steps were taken to find out how many flavonoids were in the ethanol extract as a whole. These included finding the maximum wavelength of quercetin, the operational time, making a quercetin calibration curve, and using UV-Vis spectrophotometry to determine how many flavonoids were in the extract. The analysis results reveal that the total flavonoid content in the ethanol extract of jasmine flower leaves is approximately 40.10911 ± 0.5878 mg QE/g.
Analisis kadar natrium benzoat pada kecap pedas yang beredar di pasaran dengan metode kromatografi lapis tipis (KLT) dan spektrofotometri Uv-Vis Tamara Army; Ainil Fithri Pulungan; Ridwanto Ridwanto; Anny Sartika Daulay
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.266

Abstract

Sodium benzoate is one of the preservatives allowed in food and beverages. The objective of this research was to determine the level of sodium benzoate found in spicy soy sauce. Qualitative tests are first carried out on samples with the Thin Layer Chromatography (KLT) method, and quantitative tests are carried out using the spectrophotometry Uv-Vis method to determine the preservative level of sodium benzoate. Qualitative tests on KLT plates were carried out by optimising eluents (N-Butanol: Ethyl Acetate: Ammonia, Methanol: Ethyl Acetate: Ammonia, and Isopropanol: Ammonia), where the results of the three eluents showed that the Isopropanol: Ammonia eluents had Rf values from three samples that were close to each other with the comparison Rf values. The results of quantitative analysis on spectrophotometry Uv-Vis showed the total levels of Spicy Soy Sauce A 264.16±12.3206 mcg/g, Spicy Soy Sauce B 317.8±21.8643 mcg/g and Spicy Soy Sauce C 110.2±23.5356 mcg/g. Based on these results, it can be concluded that sodium benzoate levels in samples A, B, and C meet the requirements of the maximum usage limit standard, which is not more than 600 mg/kg.  
Penetapan kadar fenolik total ekstrak etanol, fraksi etil asetat dan n-heksan daun kopi robusta (Coffea canephora Pierre ex A. Froehner) dengan metode spektrofotometri Uv-Vis Yulia Nanda Putri; Muhammad Amin Nasution; Ridwanto Ridwanto; Anny Sartika Daulay
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.301

Abstract

Phenolic compounds have various biological effects, such as antioxidant activity, can reduce the risk of cancer, coronary heart disease, stroke and other neurodegenerative diseases. Robusta coffee leaves (Coffea canephora Pierre ex A. Froehner) have activity as antioxidants because they contain abundant phenolic compounds. This study aims to determine the ratio of total phenolic levels between ethanol extract, ethyl acetate fraction and n-hexane fraction from robusta coffee leaves. In this study, the first step taken was a characterization test on simplicial powder and robusta coffee leaves macerated with 70% ethanol solvent. The maserat obtained is further fractionated with n-hexane and ethyl acetate. Followed by phytochemical screening on coffee leaf samples. Furthermore, the extract, n-hexane fraction and ethyl acetate determined total phenolic levels using visible spectrophotometry at a wavelength of 749 nm. Determination of total phenolic levels using the Folin-Ciocalteu method with gallic acid standards. Total phenolic levels are expressed in mg gallic acid equivalent (GAE) per gram of simplicial. The results showed that 70% ethanol extract of robusta coffee leaves had a total phenolic content of 25.9438± 0.0889 mg GAE/g. From the fractionation results show that the ethyl acetate fraction of robusta coffee leaves has a greater total phenolic content compared to the n-hexan fraction of 28.048 ± 0.3692 mg GAE / g and followed by the n-hexane fraction of 15.5231 ± 0.7213mg GAE / g. This is because the fractionation method can increase the desired compound content by removing or separating unwanted compounds, thus making the compound results in the use of fractions purer.
Penetapan kadar fenolik total ekstrak kayu bajakah (Spatholobus littolaris Hassk.) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri Uv-Vis Indah Triutami Harahap; Anny Sartika Daulay; Fathur Rahman; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.302

Abstract

The root of the Bajakah tampala (Spatholobus littolaris Hassk.) is one of the plants that is empirically used by the people of the interior of Central Kalimantan as a traditional medicine. According to the preliminary tests conducted, Bajakah Tampala contains phenolics. Phenolic compounds are compounds that have antioxidant activity. The objective of this research was to determine the class of compounds contained in the macerated extract and the total phenolic content and yield of the macerated extract in Bajakah roots. The stages of this research included the processing of plant materials, preparation of macerated and pirated root extracts, characterization examination, phytochemical screening, and the effect on the yield of total phenolic content of Bajakah roots using the UV-Vis spectrophotometry method. The results showed that the simplicia powder of the root of the Bajakah wood contained alkaloids, glycosides, steroids and triterpenoids, flavonoids, phenolics, saponins, and tannins, while the ethanol extract of the roots of the Bajakah wood showed the presence of alkaloids, steroids and triterpenoids, tannins, saponins, flavonoids, and phenolic compounds. The determination of total phenolic content was carried out by determining the maximum wavelength of gallic acid and calculating the total phenolic content based on the difference in ethanol concentration using the UV-Vis spectrophotometry method. The results for determining total phenolic content in 96% ethanol extract were 33.872 ± 0.0420 mg GAE/g, 70% ethanol extract was 29.345 ± 0.2149 mg GAE/g, and 50% ethanol extract was 18.512 ± 0.1355 mg GAE/g. So it can be concluded that the total phenolic content of 96% ethanol extract is higher than that of 70% ethanol extract and 50% ethanol extract.
Skrining fitokimia dan isolasi senyawa flavonoid dari ekstrak etanol biji buah menteng (Baccaurea racemosa (Reinw.) Müll.Arg). Sarmadansyah Sarmadansyah; Haris Munandar Nasution; Anny Sartika Daulay; Daeng Elysa Putri Mambang
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.304

Abstract

Menteng or kepundung is a fruit-producing tree. At first glance, Menteng or kepundung fruit resembles duku fruit with a variety of tastes (sour) and sweet. Menteng originates from Malaysia, Sumatra, Java and Bali. The purpose of this study was to determine the class of chemical compounds found in Menteng fruit seeds and to determine the characteristics of the fruit seed extract isolates by UV spectrophotometry and IR spectrophotometry. This research includes several processes, namely manufacturing simplicia from menteng fruit seeds, phytochemical screening, and simplicia characterisation. The process of making extracts from menteng fruit seed Simplicia, after that extract analysis using paper chromatography (KKt) method, then separation (isolation) of the extract with preparative paper chromatography (KKt) method, tested the purity of isolates by two-way paper chromatography (KKt), and characterisation of isolate crystals by UV spectrophotometry and IR spectrophotometry. The results of the simplicial characterisation of Menteng fruit seeds (Baccaurea racemose (Reinw.) Müll. Arg) are 4% water content, 36.81% seawater content, 8.79% ethanol soluble extract, 1.26% ash content, 1.26% ash content. Insoluble 0.5% acid. Phytochemical screening showed positive alkaloids, flavonoids, steroids/triterpenoids and glycosides, while tannins and saponins were negative. From 400 grams of simplicia, 93.3 grams of condensed extract was produced. Characterisation of isolate crystals UV spectrophotometry showed a wavelength of 280 nm, which is suspected to be a flavonoid. The results of the IR spectrophotometry showed aliphatic O-H, C-H, C=O, C-H, C-O and C=C groups.
Co-Authors Afrizani, Afrizani Akbar, Windi Hari Ali Djamhuri Alviana, Liya Anugrah, Bayu Asep Trizaldi Asparyzha, Rhyzha Azri, Atika Cici Andriani D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daeng Elysa Putri Mambang Dahnia Ayu Fitria Dalimunnthe, Gabena Indrayani Dalimunthe, Gabena Indriyani Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Eli Handayani Elma Natasya Fadillah, Nike Falah Rambe, Raudhotul Fathur Rahman Fathur Rahman Fatur Rahman fatur Rahman, fatur Fithri Pulungan, Ainil Fitri Mulyani, Fitri FRANSISKA, EVA Gabena Indrayani Dalimunthe H., Fathur Rahman Habibie, Dian Hafizha, Putri Harahap, Siti Salimah Haris Munandar Nasution Haris Munandar Nst Hasanah, Qori Hasrul Abdi Hasibuan Hastri Kholifah Helni Maulida Hindri Syahputri Ihsan Fadhilah Imelda Natasia Br S Meliala Indah Triutami Harahap Irfan Ahmad Dasuki Nasution Lastri Afni Leni Handayani Leni Handayani Lubis, Minda Sari Lulu Ilma Khoirun Nissa M Rayhan Ulmi M. Naufal Rifqi M. Pandapotan Nasution M. Pandapotan Nasution Makhfirah Manik, Umi Chairani Mardhatillah, Wulan Maya Syafira Mayang Sari Ritonga Melati Yulia Kusumastuti Merani Phaustina Lumban Gaol Minda Sari Lubis Miranza, Nona Muhammad Amin Nasution Muhammad Hizbullah Muhammad Wahyudi Munthe, Ariska Nabilla, Alfira nasution, Alfina Tri Utami Nasution, Kartika Zsaskia Nasution, Nur Sahadah Nia Novranda Pertiwi Nomi Noviani Nomi Noviani Nur Ainun Nur Hanifah Nur'ain Harahap Nurul Salsa Abya Ritonga Nurul Shaleha Pasaribu, Mesi Wilia Afrima Pasaribu, Mesi Willia Afrima Pulungan, Ainil Fitri Putri Intan Sari Putri Theresia Harianja Rahayu Rahayu Rahmadani Rahmadani Rahmah, Ruhiya Rahmi Ayusasmita Gultom Retno Sekarini Rezky , Deswita Ina Ridwanto Ridwanto Ridwanto Rika Yuliana, Rika Risma Fauziah Pasaribu Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa Safrida Safrida Sagita Marina Simatupang Sandika, M Teguh Sarmadansyah Sarmadansyah Sasnita, Merida Sekarini, Retno Selfiani Selfiani Shakila Salwa Shilvia, Fatin Siagian , Anggi Yani Sinaga, Novita Yulianti Siti Rahmasari Sri Murni Sri Wahyuni Supiyani Supiyani Supiyani, Supiyani Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah Nadia Tamara Army Tuzzahra, Safira Ulmi, M Rayhan Winda Kesuma Yayuk Putri Rahayu Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani